EY744421 | Citrus Genome Database

Overview


Name	EY744421
Unique Name	EY744421
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	996

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_PICST (ATP-dependent RNA helicase DED1 OS=Pichia stipitis GN=DED1 PE=3 SV=3)

HSP 1 Score: 113.62 bits (283), Expect = 1.079e-25
Identity = 58/125 (46.40%), Postives = 78/125 (62.40%), Query Frame = 1

Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPR---------GSRTVYPSWLLFLPPQE 579
            +++GINFD Y+DIPVE SG+ VP  + +F    L E L  NI   ++ KPTPVQ++++PI  GGRDLMACAQTGSGKT  F FP++S    E Y+  P           S  V+P+ L+  P +E
Sbjct:  142 QSSGINFDNYDDIPVEASGDGVPDPITSFTAPPLDELLVENITMSRFTKPTPVQKYSVPIVAGGRDLMACAQTGSGKTGGFLFPVLS----ESYINGPAPIAESTGAFSSHKVHPTILVMAPTRE 262

HSP 2 Score: 25.0238 bits (53), Expect = 1.079e-25
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  558 LILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            L++APTREL SQI+  +     R++  P  V GG
Sbjct:  255 LVMAPTRELVSQIYDEAKKFAYRSWVKPAVVYGG 288

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_CANAL (ATP-dependent RNA helicase DED1 OS=Candida albicans GN=DED1 PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.403e-25
Identity = 58/125 (46.40%), Postives = 79/125 (63.20%), Query Frame = 1

Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPR---------GSRTVYPSWLLFLPPQE 579
            +++GINFD Y+DIPVE SG+ VP  + +F    L E L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S    E Y++ P           S  VYP+ L+  P +E
Sbjct:  160 QSSGINFDNYDDIPVEASGDKVPEPITSFTAPPLDELLVENIQLSRFTKPTPVQKYSVPIVAAGRDLMACAQTGSGKTGGFLFPVLS----ESYMKGPAPVPESNGAFSSHKVYPTILVMAPTRE 280

HSP 2 Score: 25.0238 bits (53), Expect = 1.403e-25
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3

Query:  558 LILAPTRELSSQIHV*SPRNFPNPTGVRGGVAF 656
            L++APTREL SQI+  S + F   + VR  V +
Sbjct:  273 LVMAPTRELVSQIYEES-KKFSYRSWVRACVVY 304

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASPOR (ATP-dependent RNA helicase ded1 OS=Aspergillus oryzae GN=ded1 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.826e-25
Identity = 60/126 (47.62%), Postives = 75/126 (59.52%), Query Frame = 1

Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            ++NTGINF  Y+DIPVE SG+NVP  VN F    L + L  NI    Y  PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   +      P  +         R  YP+ L+  P +E
Sbjct:  159 KQNTGINFANYDDIPVEASGQNVPEPVNAFTNPPLDDHLIANITLAHYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAFQNGPSPTPAPASGQFSYGRQRKAYPTSLILAPTRE 284

HSP 2 Score: 25.409 bits (54), Expect = 1.826e-25
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  276 SLILAPTRELVSQI 289

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_DEBHA (ATP-dependent RNA helicase DED1 OS=Debaryomyces hansenii GN=DED1 PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.379e-25
Identity = 59/132 (44.70%), Postives = 79/132 (59.85%), Query Frame = 1

Query:  211 EQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPR---------GSRTVYPSWLLFLPPQE 579
            E+P  +  ++GINFD Y+DIPVE SGE VP  +  F    L   L  N++  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S    E Y+  P           S  VYP+ L+  P +E
Sbjct:  125 EEPTTQ--SSGINFDNYDDIPVEASGEGVPEPITAFTAPPLDPLLVENVKLSRFTKPTPVQKYSVPIVAAGRDLMACAQTGSGKTGGFLFPVLS----ESYMNGPAEVPETAGAFSSHKVYPTALVMAPTRE 250

HSP 2 Score: 25.7942 bits (55), Expect = 2.379e-25
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            AL++APTREL SQI   +     R++  P+ V GG
Sbjct:  242 ALVMAPTRELVSQIFDEAKKFAYRSWVRPSVVYGG 276

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_BOTFB (ATP-dependent RNA helicase ded1 OS=Botryotinia fuckeliana (strain B05.10) GN=ded1 PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.092e-25
Identity = 64/143 (44.76%), Postives = 81/143 (56.64%), Query Frame = 1

Query:  172 EREVNPFGDDVGAEQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-------SRTVYPSWLLFLPPQE 579
            ERE+    DD   +Q       TGINF+ Y+DIPVE SG++VP  V  F    L + L  NI    Y  PTPVQ+++IPI +GGRDLMACAQTGSGKT  F FPI+S   +      P         +R  YP+ L+  P +E
Sbjct:  147 ERELFGIADDPTKQQ-------TGINFEKYDDIPVEASGQDVPEPVLKFTNPPLDDHLIKNIELAHYKVPTPVQKYSIPIVMGGRDLMACAQTGSGKTGGFLFPILSQAFQTGPSPVPANAAGSFGRTRKAYPTSLILAPTRE 282

HSP 2 Score: 26.9498 bits (58), Expect = 3.092e-25
Identity = 18/35 (51.43%), Postives = 22/35 (62.86%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            +LILAPTREL SQI+  S     R++  P  V GG
Sbjct:  274 SLILAPTRELVSQIYDESRKFAYRSWVRPCVVYGG 308

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_GIBZE (ATP-dependent RNA helicase DED1 OS=Gibberella zeae GN=DED1 PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.093e-25
Identity = 68/147 (46.26%), Postives = 86/147 (58.50%), Query Frame = 1

Query:  172 EREVNPFGDDVGAEQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-----------SRTVYPSWLLFLPPQE 579
            ERE+  FG  V AE P   +++TGINF+ Y+DIPVE SG +VP  V+ F    L E L  NI    Y  PTPVQ+++IPI  GGRDLMACAQTGSGKT  F FPI+S    + ++  P              R  YP+ L+  P +E
Sbjct:  146 EREL--FG--VTAEDP--SKQHTGINFEKYDDIPVEASGTDVPEPVHQFTTPPLDEHLCRNIELAHYKVPTPVQKYSIPIVSGGRDLMACAQTGSGKTGGFLFPILS----QAFINGPSAVPANAAGQFGRQRKAYPTSLILAPTRE 282

HSP 2 Score: 25.7942 bits (55), Expect = 3.093e-25
Identity = 18/35 (51.43%), Postives = 21/35 (60.00%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            +LILAPTREL SQI   S     R++  P  V GG
Sbjct:  274 SLILAPTRELVSQIFDESRKFAYRSWVRPCVVYGG 308

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_YARLI (ATP-dependent RNA helicase DED1 OS=Yarrowia lipolytica GN=DED1 PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.097e-25
Identity = 57/122 (46.72%), Postives = 76/122 (62.30%), Query Frame = 1

Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISG--IMREQYVQRPRGSR----TVYPSWLLFLPPQE 579
            ++TGINFD Y++IPVE +G +VP  +N F    L E L  NI+  +Y KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S           +P G R      YP+ L+  P +E
Sbjct:  134 QSTGINFDNYDEIPVEATGNDVPEPINAFTSPPLEEHLLTNIKLARYNKPTPVQKYSVPIVAAGRDLMACAQTGSGKTGGFLFPVLSQSFFHGPSPTPQPTGPRHMHKKAYPTALVLAPTRE 255

HSP 2 Score: 26.9498 bits (58), Expect = 3.097e-25
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  555 ALILAPTRELSSQIH 599
            AL+LAPTREL SQI+
Sbjct:  247 ALVLAPTRELVSQIY 261

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_COCIM (ATP-dependent RNA helicase DED1 OS=Coccidioides immitis GN=DED1 PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 5.240e-25
Identity = 59/126 (46.83%), Postives = 76/126 (60.32%), Query Frame = 1

Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            +++TGINF  Y+DIPVE SG +VP  V TF    L + L  NI+   Y  PTPVQ+++IPI +GGRDLMACAQTGSGKT  F FPI+S   +      P  +         R  YP+ L+  P +E
Sbjct:  148 KQHTGINFANYDDIPVEASGHDVPEPVTTFTNPPLDDHLISNIKLATYKTPTPVQKYSIPIVMGGRDLMACAQTGSGKTGGFLFPILSQAFKNGPSAVPTQNANQFSYGRQRKAYPTSLILAPTRE 273

HSP 2 Score: 26.1794 bits (56), Expect = 5.240e-25
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  555 ALILAPTRELSSQIH 599
            +LILAPTREL SQI+
Sbjct:  265 SLILAPTRELVSQIY 279

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_SCLS1 (ATP-dependent RNA helicase ded1 OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) GN=ded1 PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 6.817e-25
Identity = 64/143 (44.76%), Postives = 81/143 (56.64%), Query Frame = 1

Query:  172 EREVNPFGDDVGAEQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-------SRTVYPSWLLFLPPQE 579
            ERE+    DD   +Q       TGINF+ Y+DIPVE SG++VP  V  F    L + L  NI    Y  PTPVQ+++IPI +GGRDLMACAQTGSGKT  F FPI+S   +      P         +R  YP+ L+  P +E
Sbjct:  147 ERELFGIADDPTKQQ-------TGINFEKYDDIPVEASGQDVPEPVLKFTNPPLDDHLIKNIELAHYKVPTPVQKYSIPIVMGGRDLMACAQTGSGKTGGFLFPILSQAFQTGPSPIPANAAGSFGRTRKAYPTSLILAPTRE 282

HSP 2 Score: 25.7942 bits (55), Expect = 6.817e-25
Identity = 18/35 (51.43%), Postives = 21/35 (60.00%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            +LILAPTREL SQI   S     R++  P  V GG
Sbjct:  274 SLILAPTRELVSQIFDESRKFAYRSWVRPCVVYGG 308

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASPTN (ATP-dependent RNA helicase ded1 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=ded1 PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.817e-25
Identity = 59/126 (46.83%), Postives = 77/126 (61.11%), Query Frame = 1

Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMRE--QYVQRPRG-------SRTVYPSWLLFLPPQE 579
            +++TGINF  Y+DIPVE SG++VP  VN F    L + L  NI   +Y+ PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   +        P G        R  YP+ L+  P +E
Sbjct:  159 KQSTGINFANYDDIPVEASGQDVPEPVNAFTNPPLDDHLISNIALARYLTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAFQNGPSPTPAPAGGQLGYGRQRKAYPTSLILAPTRE 284

HSP 2 Score: 25.409 bits (54), Expect = 6.817e-25
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  276 SLILAPTRELVSQI 289

The following BLAST results are available for this feature:

BLAST of EY744421 vs. ExPASy Swiss-Prot

BLAST of EY744421 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
DED1_PICST	1.079e-25	46.40	ATP-dependent RNA helicase DED1 OS=Pichia stipitis...	[more]
DED1_CANAL	1.403e-25	46.40	ATP-dependent RNA helicase DED1 OS=Candida albican...	[more]
DED1_ASPOR	1.826e-25	47.62	ATP-dependent RNA helicase ded1 OS=Aspergillus ory...	[more]
DED1_DEBHA	2.379e-25	44.70	ATP-dependent RNA helicase DED1 OS=Debaryomyces ha...	[more]
DED1_BOTFB	3.092e-25	44.76	ATP-dependent RNA helicase ded1 OS=Botryotinia fuc...	[more]
DED1_GIBZE	3.093e-25	46.26	ATP-dependent RNA helicase DED1 OS=Gibberella zeae...	[more]
DED1_YARLI	3.097e-25	46.72	ATP-dependent RNA helicase DED1 OS=Yarrowia lipoly...	[more]
DED1_COCIM	5.240e-25	46.83	ATP-dependent RNA helicase DED1 OS=Coccidioides im...	[more]
DED1_SCLS1	6.817e-25	44.76	ATP-dependent RNA helicase ded1 OS=Sclerotinia scl...	[more]
DED1_ASPTN	6.817e-25	46.83	ATP-dependent RNA helicase ded1 OS=Aspergillus ter...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-008-D10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744421 ID=EY744421; Name=EY744421; organism=Citrus sinensis; type=EST; length=996bp
CCACCAACCTCTTCTGAACCTCCTGCTTCGTCACGAGAATCTACTGAACC
TGCCAGTGGCCCCGGCTGGGGCAGTGGTTCGAGGCCCGATTTTGGGCGTG
GTCAGGGTTACGGCTCTGGTGGTAGAAGTGGCGGTGGTTGGAATAATAGA
AGTGGGGGTTGGGACCGAAGGGAACGTGAAGTGAACCCGTTTGGTGATGA
TGTTGGTGCAGAGCAGCCGGTTGCTGAAGAAGAAAACACGGGTATTAATT
TTGATGCTTATGAGGATATTCCAGTGGAGACAAGTGGAGAAAATGTGCCA
CCGGCAGTTAATACATTTGCAGAAATTGATTTGGGGGAAGCTTTGAATCT
TAATATTAGGAGGTGCAAGTATGTCAAACCGACGCCTGTTCAGCGTCATG
CGATTCCTATATCGATTGGTGGGCGAGATTTAATGGCCTGTGCTCAGACA
GGGTCGGGGAAGACAGCTGCCTTTTGTTTTCCAATTATTAGTGGGATTAT
GCGTGAGCAGTACGTACAGAGACCACGTGGATCGCGGACTGTTTATCCCT
CCTGGCTCTTATTCTTGCCCCCACAAGAGAACTCTCGTCTCAGATCCATG
TTTGAAGCCCCAGAAATTTTCCTAATCCAACTGGGGTCAGGGGGGGGGTT
GCTTTCCGAAGGAACCACCATTCAACCCACCATTTGCGAAAACTTTTAAA
AAAAGGGGTTGAATATTCCTTTGGCCAACCCCCGCGAAAAATGGGTTTGA
TTCGCGTTGAAAAAACCTAAAATTTCTCTTTGCAAAAAGATTAGAATCCT
TTACCGCTTCCAATGAAGGGCAGACGCGGAGGCTGGATATTGGGGGTTTT
TGGCCCCCCGCAACGCCAAACAATATTTGCTGCCCCACCAACGGGTCTTA
TGCCCTCCCCCCCACCTGTGGGTTACAAACAAAATAACAACGGGCTCTTT
TCTTCCGGGCTGCCCCACCTTCCTCCCTCACAAAAAAACAATACTA