EY744421

Overview
NameEY744421
Unique NameEY744421
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length996
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASPCL (ATP-dependent RNA helicase ded1 OS=Aspergillus clavatus GN=ded1 PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 8.870e-25
Identity = 58/126 (46.03%), Postives = 78/126 (61.90%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            +++TGINF  Y+DIPVE SG++VP  VN F    L + L  NI+  +Y  PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   ++     P  +         R  YP+ L+  P +E
Sbjct:  162 KQSTGINFANYDDIPVEASGQDVPEPVNAFTNPPLDDHLISNIKLARYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAFQKGPSAVPAQASGQMSYGRQRKAYPTSLILAPTRE 287

HSP 2 Score: 25.409 bits (54), Expect = 8.870e-25
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  279 SLILAPTRELVSQI 292
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_VANPO (ATP-dependent RNA helicase DED1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) GN=DED1 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 8.877e-25
Identity = 54/121 (44.63%), Postives = 74/121 (61.16%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGSR-----TVYPSWLLFLPPQE 579
            +++GINFD Y+DIPV+ SG +VP A+  F    L   L  NI   ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S   +      P  +R       YP+ L+  P +E
Sbjct:  134 QSSGINFDNYDDIPVDASGTDVPEAITEFTSPPLDALLLENIILARFTKPTPVQKYSVPIVSRGRDLMACAQTGSGKTGGFLFPVLSESFKNGPSPMPESARKSFVKKAYPTALVLAPTRE 254

HSP 2 Score: 29.261 bits (64), Expect = 8.877e-25
Identity = 17/35 (48.57%), Postives = 24/35 (68.57%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGG 647
            AL+LAPTREL++QI+  +     R++  PT V GG
Sbjct:  246 ALVLAPTRELATQIYDEAKKFTYRSWVRPTVVYGG 280
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_AJECN (ATP-dependent RNA helicase DED1 OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=DED1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.154e-24
Identity = 65/141 (46.10%), Postives = 87/141 (61.70%), Query Frame = 1 
Query:  172 EREVNPFGDDVGAEQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIM--REQYVQRPRG---SRTVYPSWLLFLPPQE 579
            ERE+  FG+   A+ P   +++TGINF  Y+ IPVE SG +VP ++  F    L E L  NI   +Y  PTPVQ+++IPI +GGRDLMACAQTGSGKT  F FPI+S  +  R    +  RG    +  YP+ L+  P +E
Sbjct:  164 EREL--FGE---ADDPT--KQHTGINFSNYDSIPVEASGHDVPESITAFTNPPLHEHLLSNIVLARYTVPTPVQKYSIPIVMGGRDLMACAQTGSGKTGGFLFPILSQSLHTRGPEAEAARGLGRQQKAYPTALILGPTRE 297

HSP 2 Score: 25.7942 bits (55), Expect = 1.154e-24
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIH 599
            ALIL PTREL SQI+
Sbjct:  289 ALILGPTRELVSQIY 303
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASPNC (ATP-dependent RNA helicase ded1 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN=ded1 PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.154e-24
Identity = 59/126 (46.83%), Postives = 75/126 (59.52%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            ++NTGINF  Y+DIPVE SG +VP  VN F    L + L  NI+   Y  PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   +      P  +         R  YP+ L+  P +E
Sbjct:  160 KQNTGINFANYDDIPVEASGHDVPEPVNAFTNPPLDDHLIENIKLAHYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAYQNGPSAAPAQAGGQFGYGRQRKAYPTSLILAPTRE 285

HSP 2 Score: 25.409 bits (54), Expect = 1.154e-24
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  277 SLILAPTRELVSQI 290
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_CHAGB (ATP-dependent RNA helicase DED1 OS=Chaetomium globosum GN=DED1 PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.502e-24
Identity = 59/127 (46.46%), Postives = 75/127 (59.06%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPR----------GSRTVYPSWLLFLPPQE 579
            +++TGINF+ Y+DIPV  SG +VP  V TF+   L   L  NI   +Y  PTPVQ+++IPI I GRDLMACAQTGSGKT  F FP    IM + + Q P             R  YP+ L+  P +E
Sbjct:  171 KQHTGINFEKYDDIPVNPSGRDVPEPVLTFSNPPLDAHLLSNIELARYQIPTPVQKYSIPIVINGRDLMACAQTGSGKTGGFLFP----IMHQSFTQGPSPIPAQSGGGYRQRKAYPTALILAPTRE 293

HSP 2 Score: 27.7202 bits (60), Expect = 1.502e-24
Identity = 18/34 (52.94%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAF 656
            ALILAPTREL SQI+    R F   + VR  V +
Sbjct:  285 ALILAPTRELVSQIYE-EARKFAYRSWVRPCVVY 317
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_KLULA (ATP-dependent RNA helicase DED1 OS=Kluyveromyces lactis GN=DED1 PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.504e-24
Identity = 56/128 (43.75%), Postives = 79/128 (61.72%), Query Frame = 1 
Query:  220 VAEE---ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS-----RTVYPSWLLFLPPQE 579
            VAE+   +++GINFD Y+DIPVE SG +VP  ++ F    L   L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S          P  +     + VYP+ ++  P +E
Sbjct:  126 VAEDKSFQSSGINFDNYDDIPVEASGNDVPEPISEFHSPPLDPLLLDNIKLARFTKPTPVQKYSVPIVAAGRDLMACAQTGSGKTGGFLFPVLSESFSSGPASTPEAAGNSYIKKVYPTAVILAPTRE 253

HSP 2 Score: 26.5646 bits (57), Expect = 1.504e-24
Identity = 16/37 (43.24%), Postives = 24/37 (64.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIHV*SP----RNFPNPTGVRGGVA 653
            A+ILAPTREL++QI+  +     R++  P  V GG +
Sbjct:  245 AVILAPTRELATQIYDEAKKFTYRSWVKPMVVYGGAS 281
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP1_CANGA (ATP-dependent RNA helicase DBP1 OS=Candida glabrata GN=DBP1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.959e-24
Identity = 57/119 (47.90%), Postives = 72/119 (60.50%), Query Frame = 1 
Query:  238 TGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS-----RTVYPSWLLFLPPQE 579
            +GINFD Y+DIPVE SG+NVP A+  F    L E L  N+    + KPTPVQ+++IPI    RDLMACAQTGSGKT  F FP++S +        P  +     R  YPS L+  P +E
Sbjct:  114 SGINFDNYDDIPVEASGDNVPEAITEFKSPPLDELLLENVELANFSKPTPVQKYSIPIVTKNRDLMACAQTGSGKTGGFLFPVLSELFLNGPAPLPEHTRHSYMRKCYPSALVLAPTRE 232

HSP 2 Score: 25.0238 bits (53), Expect = 1.959e-24
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            AL+LAPTREL+ QI
Sbjct:  224 ALVLAPTRELAIQI 237
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_NEOFI (ATP-dependent RNA helicase ded1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=ded1 PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.544e-24
Identity = 58/126 (46.03%), Postives = 76/126 (60.32%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            +++TGINF  Y+DIPVE SG +VP  VN F    L + L  NI+  +Y  PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   +      P  +         R  YP+ L+  P +E
Sbjct:  163 KQSTGINFANYDDIPVEASGHDVPEPVNAFTNPPLDDHLISNIKLARYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAFQTGPSAVPAQASGQFGYGRQRKAYPTSLILAPTRE 288

HSP 2 Score: 25.409 bits (54), Expect = 2.544e-24
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  280 SLILAPTRELVSQI 293
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASPFU (ATP-dependent RNA helicase ded1 OS=Aspergillus fumigatus GN=ded1 PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.544e-24
Identity = 58/126 (46.03%), Postives = 76/126 (60.32%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS---------RTVYPSWLLFLPPQE 579
            +++TGINF  Y+DIPVE SG +VP  VN F    L + L  NI+  +Y  PTPVQ+++IPI + GRDLMACAQTGSGKT  F FPI+S   +      P  +         R  YP+ L+  P +E
Sbjct:  161 KQSTGINFANYDDIPVEASGHDVPEPVNAFTNPPLDDHLISNIKLARYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGFLFPILSQAFQTGPSPVPAQASGQFGYGRQRKAYPTSLILAPTRE 286

HSP 2 Score: 25.409 bits (54), Expect = 2.544e-24
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  278 SLILAPTRELVSQI 291
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_USTMA (ATP-dependent RNA helicase DED1 OS=Ustilago maydis GN=DED1 PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.545e-24
Identity = 56/124 (45.16%), Postives = 71/124 (57.26%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMRE--------QYVQRPRGSRTVYPSWLLFLPPQE 579
            ++ GINFD Y DIPVE +G +VP  V TF    +   L  NI+  +Y  PTPVQ+++IPI   GRDLM CAQTGSGKT  F FPI+S +           +  Q     R  YPS L+  P +E
Sbjct:  163 QSMGINFDKYGDIPVEATGRDVPEPVTTFTSPPIDAHLLENIKLARYTNPTPVQKYSIPIIKLGRDLMGCAQTGSGKTGGFLFPILSALFTHGPPPPSAAEMAQGGYNRRKAYPSTLILAPTRE 286

HSP 2 Score: 28.1054 bits (61), Expect = 2.545e-24
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  558 LILAPTRELSSQIH 599
            LILAPTREL SQIH
Sbjct:  279 LILAPTRELVSQIH 292
The following BLAST results are available for this feature:
BLAST of EY744421 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133
Match NameE-valueIdentityDescription
DED1_ASPCL8.870e-2546.03ATP-dependent RNA helicase ded1 OS=Aspergillus cla... [more]
DED1_VANPO8.877e-2544.63ATP-dependent RNA helicase DED1 OS=Vanderwaltozyma... [more]
DED1_AJECN1.154e-2446.10ATP-dependent RNA helicase DED1 OS=Ajellomyces cap... [more]
DED1_ASPNC1.154e-2446.83ATP-dependent RNA helicase ded1 OS=Aspergillus nig... [more]
DED1_CHAGB1.502e-2446.46ATP-dependent RNA helicase DED1 OS=Chaetomium glob... [more]
DED1_KLULA1.504e-2443.75ATP-dependent RNA helicase DED1 OS=Kluyveromyces l... [more]
DBP1_CANGA1.959e-2447.90ATP-dependent RNA helicase DBP1 OS=Candida glabrat... [more]
DED1_NEOFI2.544e-2446.03ATP-dependent RNA helicase ded1 OS=Neosartorya fis... [more]
DED1_ASPFU2.544e-2446.03ATP-dependent RNA helicase ded1 OS=Aspergillus fum... [more]
DED1_USTMA2.545e-2445.16ATP-dependent RNA helicase DED1 OS=Ustilago maydis... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744421 ID=EY744421; Name=EY744421; organism=Citrus sinensis; type=EST; length=996bp
CCACCAACCTCTTCTGAACCTCCTGCTTCGTCACGAGAATCTACTGAACC
TGCCAGTGGCCCCGGCTGGGGCAGTGGTTCGAGGCCCGATTTTGGGCGTG
GTCAGGGTTACGGCTCTGGTGGTAGAAGTGGCGGTGGTTGGAATAATAGA
AGTGGGGGTTGGGACCGAAGGGAACGTGAAGTGAACCCGTTTGGTGATGA
TGTTGGTGCAGAGCAGCCGGTTGCTGAAGAAGAAAACACGGGTATTAATT
TTGATGCTTATGAGGATATTCCAGTGGAGACAAGTGGAGAAAATGTGCCA
CCGGCAGTTAATACATTTGCAGAAATTGATTTGGGGGAAGCTTTGAATCT
TAATATTAGGAGGTGCAAGTATGTCAAACCGACGCCTGTTCAGCGTCATG
CGATTCCTATATCGATTGGTGGGCGAGATTTAATGGCCTGTGCTCAGACA
GGGTCGGGGAAGACAGCTGCCTTTTGTTTTCCAATTATTAGTGGGATTAT
GCGTGAGCAGTACGTACAGAGACCACGTGGATCGCGGACTGTTTATCCCT
CCTGGCTCTTATTCTTGCCCCCACAAGAGAACTCTCGTCTCAGATCCATG
TTTGAAGCCCCAGAAATTTTCCTAATCCAACTGGGGTCAGGGGGGGGGTT
GCTTTCCGAAGGAACCACCATTCAACCCACCATTTGCGAAAACTTTTAAA
AAAAGGGGTTGAATATTCCTTTGGCCAACCCCCGCGAAAAATGGGTTTGA
TTCGCGTTGAAAAAACCTAAAATTTCTCTTTGCAAAAAGATTAGAATCCT
TTACCGCTTCCAATGAAGGGCAGACGCGGAGGCTGGATATTGGGGGTTTT
TGGCCCCCCGCAACGCCAAACAATATTTGCTGCCCCACCAACGGGTCTTA
TGCCCTCCCCCCCACCTGTGGGTTACAAACAAAATAACAACGGGCTCTTT
TCTTCCGGGCTGCCCCACCTTCCTCCCTCACAAAAAAACAATACTA
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