EY744421

Overview
NameEY744421
Unique NameEY744421
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length996
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_SCHPO (ATP-dependent RNA helicase ded1 OS=Schizosaccharomyces pombe GN=ded1 PE=1 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.547e-24
Identity = 58/123 (47.15%), Postives = 74/123 (60.16%), Query Frame = 1 
Query:  235 NTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIIS--------GIMREQYVQRPRGSRTVYPSWLLFLPPQE 579
            +TGINF+ Y+DIPVE SG ++ P VN F    L   L  NI+   Y +PTPVQ+++IPI   GRDLMACAQTGSGKTA F FPI+S         +  +Q        R  YP+ L+  P +E
Sbjct:  145 STGINFEKYDDIPVEVSGGDIEP-VNEFTSPPLNSHLLQNIKLSGYTQPTPVQKNSIPIVTSGRDLMACAQTGSGKTAGFLFPILSLAFDKGPAAVPVDQDAGMGYRPRKAYPTTLILAPTRE 266

HSP 2 Score: 29.6462 bits (65), Expect = 2.547e-24
Identity = 19/38 (50.00%), Postives = 21/38 (55.26%), Query Frame = 3 
Query:  558 LILAPTRELSSQIHV*SP----RNFPNPTGVRGGVAFR 659
            LILAPTREL  QIH  S     R++  P  V GG   R
Sbjct:  259 LILAPTRELVCQIHEESRKFCYRSWVRPCAVYGGADIR 296
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_PHANO (ATP-dependent RNA helicase DED1 OS=Phaeosphaeria nodorum GN=DED1 PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.306e-24
Identity = 57/124 (45.97%), Postives = 73/124 (58.87%), Query Frame = 1 
Query:  229 EENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-------SRTVYPSWLLFLPPQE 579
            ++ TGINF+ Y+DIPVE SG+ VP  V  F    L + L  NI    Y  PTPVQ+++IPI +GGRDLMACAQTGSGKT  F FPI++   +      P          R  YP+ L+  P +E
Sbjct:  174 KQQTGINFEKYDDIPVEASGQGVPEPVTRFTNPPLDDHLLSNIELSGYKVPTPVQKYSIPIVMGGRDLMACAQTGSGKTGGFLFPILAQAFQNGPSPPPTAQAGGYGRQRKAYPTSLILAPTRE 297

HSP 2 Score: 25.409 bits (54), Expect = 4.306e-24
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            +LILAPTREL SQI
Sbjct:  289 SLILAPTRELVSQI 302
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_PICGU (ATP-dependent RNA helicase DED1 OS=Pichia guilliermondii GN=DED1 PE=3 SV=3)

HSP 1 Score: 107.071 bits (266), Expect = 5.612e-24
Identity = 57/132 (43.18%), Postives = 81/132 (61.36%), Query Frame = 1 
Query:  220 VAEE---ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPR---------GSRTVYPSWLLFLPPQE 579
            VAE+   +++GINFD Y+DIPVE +G+ VP  ++ F    L   +  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S    E Y+  P           S  V+P+ L+  P +E
Sbjct:  136 VAEDPSVQSSGINFDNYDDIPVEATGDGVPEPISAFTAPPLDPLIVENIKLSRFTKPTPVQKYSVPIVASGRDLMACAQTGSGKTGGFLFPVLS----ESYMNGPDAVPESQGAFSSHKVHPTALVMAPTRE 263

HSP 2 Score: 25.7942 bits (55), Expect = 5.612e-24
Identity = 17/35 (48.57%), Postives = 21/35 (60.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI----HV*SPRNFPNPTGVRGG 647
            AL++APTREL SQI       S R++  P  V GG
Sbjct:  255 ALVMAPTRELVSQIFEEAKKFSYRSWVRPCVVYGG 289
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_ASHGO (ATP-dependent RNA helicase DED1 OS=Ashbya gossypii GN=DED1 PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 6.880e-24
Identity = 69/188 (36.70%), Postives = 103/188 (54.79%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGS----RTVYPSWLLFLPPQENSRLRSMFEAPEIFLIQXXXXXXXXXXXTTIQPTICENF*KKGLNIPLANPREKWV*FALKKPKISLC 783
            +++GINFD Y+DIPV+ SGE+VP  +  F    L E L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S          P  S    R  YP+ ++  P +E +    +F+  + F  +             I+  I E   ++G ++ +A P    +   L++ KISLC
Sbjct:  120 QSSGINFDNYDDIPVDASGEDVPDPITEFTSPPLDELLLENIKLARFTKPTPVQKYSVPIVAKGRDLMACAQTGSGKTGGFLFPVLSQSFSNGPASTPDESGYYMRKAYPTAVVLAPTRELA--TQIFDEAKKFTYRSWVKPCVVYGGADIRQQIREL--ERGCDLIVATPGR--LNDLLERGKISLC 301
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP1_YEAS7 (ATP-dependent RNA helicase DBP1 OS=Saccharomyces cerevisiae (strain YJM789) GN=DBP1 PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 9.508e-24
Identity = 56/120 (46.67%), Postives = 74/120 (61.67%), Query Frame = 1 
Query:  235 NTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-----SRTVYPSWLLFLPPQE 579
            ++GI FD Y+DIPV+ SG++VP  +  F+   L E L  NI+   + KPTPVQ+++IPI   GRDLMACAQTGSGKT  F FP+ + + R      P       SR  YPS L+  P +E
Sbjct:  129 SSGIKFDNYDDIPVDASGKDVPEPILDFSSPPLDELLMENIKLASFTKPTPVQKYSIPIVTKGRDLMACAQTGSGKTGGFLFPLFTELFRSGPSPVPEKAQSFYSRKGYPSALVLAPTRE 248

HSP 2 Score: 26.1794 bits (56), Expect = 9.508e-24
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            AL+LAPTREL++QI
Sbjct:  240 ALVLAPTRELATQI 253
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_YEAST (ATP-dependent RNA helicase DED1 OS=Saccharomyces cerevisiae GN=DED1 PE=1 SV=2)

HSP 1 Score: 107.071 bits (266), Expect = 1.238e-23
Identity = 52/121 (42.98%), Postives = 75/121 (61.98%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-----SRTVYPSWLLFLPPQE 579
            +++GINFD Y+DIPV+ SG++VP  +  F    L   L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S   +     +P        R  YP+ ++  P +E
Sbjct:  116 QSSGINFDNYDDIPVDASGKDVPEPITEFTSPPLDGLLLENIKLARFTKPTPVQKYSVPIVANGRDLMACAQTGSGKTGGFLFPVLSESFKTGPSPQPESQGSFYQRKAYPTAVIMAPTRE 236

HSP 2 Score: 24.6386 bits (52), Expect = 1.238e-23
Identity = 10/14 (71.43%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            A+I+APTREL++QI
Sbjct:  228 AVIMAPTRELATQI 241
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_YEAS7 (ATP-dependent RNA helicase DED1 OS=Saccharomyces cerevisiae (strain YJM789) GN=DED1 PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.238e-23
Identity = 52/121 (42.98%), Postives = 75/121 (61.98%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-----SRTVYPSWLLFLPPQE 579
            +++GINFD Y+DIPV+ SG++VP  +  F    L   L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S   +     +P        R  YP+ ++  P +E
Sbjct:  116 QSSGINFDNYDDIPVDASGKDVPEPITEFTSPPLDGLLLENIKLARFTKPTPVQKYSVPIVANGRDLMACAQTGSGKTGGFLFPVLSESFKTGPSPQPESQGSFYQRKAYPTAVIMAPTRE 236

HSP 2 Score: 24.6386 bits (52), Expect = 1.238e-23
Identity = 10/14 (71.43%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            A+I+APTREL++QI
Sbjct:  228 AVIMAPTRELATQI 241
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DED1_CANGA (ATP-dependent RNA helicase DED1 OS=Candida glabrata GN=DED1 PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.610e-23
Identity = 49/87 (56.32%), Postives = 64/87 (73.56%), Query Frame = 1 
Query:  232 ENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIIS 492
            +++GINFD Y+DIPVE SGE+VP A+  F    L   L  NI+  ++ KPTPVQ++++PI   GRDLMACAQTGSGKT  F FP++S
Sbjct:  119 QSSGINFDNYDDIPVEASGEDVPEAITEFTSPPLDSLLLENIKLARFTKPTPVQKYSVPIVSKGRDLMACAQTGSGKTGGFLFPVLS 205

HSP 2 Score: 24.2534 bits (51), Expect = 1.610e-23
Identity = 9/14 (64.29%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            A+++APTREL++QI
Sbjct:  231 AVVMAPTRELATQI 244
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP1_YEAST (ATP-dependent RNA helicase DBP1 OS=Saccharomyces cerevisiae GN=DBP1 PE=1 SV=2)

HSP 1 Score: 103.99 bits (258), Expect = 3.546e-23
Identity = 55/120 (45.83%), Postives = 74/120 (61.67%), Query Frame = 1 
Query:  235 NTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRG-----SRTVYPSWLLFLPPQE 579
            ++GI FD Y++IPV+ SG++VP  +  F+   L E L  NI+   + KPTPVQ+++IPI   GRDLMACAQTGSGKT  F FP+ + + R      P       SR  YPS L+  P +E
Sbjct:  129 SSGIKFDNYDNIPVDASGKDVPEPILDFSSPPLDELLMENIKLASFTKPTPVQKYSIPIVTKGRDLMACAQTGSGKTGGFLFPLFTELFRSGPSPVPEKAQSFYSRKGYPSALVLAPTRE 248

HSP 2 Score: 26.1794 bits (56), Expect = 3.546e-23
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            AL+LAPTREL++QI
Sbjct:  240 ALVLAPTRELATQI 253
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: VASA_DROME (ATP-dependent RNA helicase vasa OS=Drosophila melanogaster GN=vas PE=1 SV=3)

HSP 1 Score: 92.8189 bits (229), Expect = 1.006e-18
Identity = 42/92 (45.65%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:  238 TGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQY 513
            +GI+F  Y +IPV+ +G +VP  +  F   DL + +  N+ +  Y  PTP+Q+ +IP+   GRDLMACAQTGSGKTAAF  PI+S ++ + +
Sbjct:  221 SGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPH 312

HSP 2 Score: 22.3274 bits (46), Expect = 1.006e-18
Identity = 16/65 (24.62%), Postives = 30/65 (46.15%), Query Frame = 3 
Query:  558 LILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLI 752
            +I++PTREL+ QI     R F   + ++ G+ +     S  H  + + +G       P R +  +
Sbjct:  322 VIVSPTRELAIQIFN-EARKFAFESYLKIGIVY--GGTSFRHQNECITRGCHVVIATPGRLLDFV 383
The following BLAST results are available for this feature:
BLAST of EY744421 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133
Match NameE-valueIdentityDescription
DED1_SCHPO2.547e-2447.15ATP-dependent RNA helicase ded1 OS=Schizosaccharom... [more]
DED1_PHANO4.306e-2445.97ATP-dependent RNA helicase DED1 OS=Phaeosphaeria n... [more]
DED1_PICGU5.612e-2443.18ATP-dependent RNA helicase DED1 OS=Pichia guillier... [more]
DED1_ASHGO6.880e-2436.70ATP-dependent RNA helicase DED1 OS=Ashbya gossypii... [more]
DBP1_YEAS79.508e-2446.67ATP-dependent RNA helicase DBP1 OS=Saccharomyces c... [more]
DED1_YEAST1.238e-2342.98ATP-dependent RNA helicase DED1 OS=Saccharomyces c... [more]
DED1_YEAS71.238e-2342.98ATP-dependent RNA helicase DED1 OS=Saccharomyces c... [more]
DED1_CANGA1.610e-2356.32ATP-dependent RNA helicase DED1 OS=Candida glabrat... [more]
DBP1_YEAST3.546e-2345.83ATP-dependent RNA helicase DBP1 OS=Saccharomyces c... [more]
VASA_DROME1.006e-1845.65ATP-dependent RNA helicase vasa OS=Drosophila mela... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744421 ID=EY744421; Name=EY744421; organism=Citrus sinensis; type=EST; length=996bp
CCACCAACCTCTTCTGAACCTCCTGCTTCGTCACGAGAATCTACTGAACC
TGCCAGTGGCCCCGGCTGGGGCAGTGGTTCGAGGCCCGATTTTGGGCGTG
GTCAGGGTTACGGCTCTGGTGGTAGAAGTGGCGGTGGTTGGAATAATAGA
AGTGGGGGTTGGGACCGAAGGGAACGTGAAGTGAACCCGTTTGGTGATGA
TGTTGGTGCAGAGCAGCCGGTTGCTGAAGAAGAAAACACGGGTATTAATT
TTGATGCTTATGAGGATATTCCAGTGGAGACAAGTGGAGAAAATGTGCCA
CCGGCAGTTAATACATTTGCAGAAATTGATTTGGGGGAAGCTTTGAATCT
TAATATTAGGAGGTGCAAGTATGTCAAACCGACGCCTGTTCAGCGTCATG
CGATTCCTATATCGATTGGTGGGCGAGATTTAATGGCCTGTGCTCAGACA
GGGTCGGGGAAGACAGCTGCCTTTTGTTTTCCAATTATTAGTGGGATTAT
GCGTGAGCAGTACGTACAGAGACCACGTGGATCGCGGACTGTTTATCCCT
CCTGGCTCTTATTCTTGCCCCCACAAGAGAACTCTCGTCTCAGATCCATG
TTTGAAGCCCCAGAAATTTTCCTAATCCAACTGGGGTCAGGGGGGGGGTT
GCTTTCCGAAGGAACCACCATTCAACCCACCATTTGCGAAAACTTTTAAA
AAAAGGGGTTGAATATTCCTTTGGCCAACCCCCGCGAAAAATGGGTTTGA
TTCGCGTTGAAAAAACCTAAAATTTCTCTTTGCAAAAAGATTAGAATCCT
TTACCGCTTCCAATGAAGGGCAGACGCGGAGGCTGGATATTGGGGGTTTT
TGGCCCCCCGCAACGCCAAACAATATTTGCTGCCCCACCAACGGGTCTTA
TGCCCTCCCCCCCACCTGTGGGTTACAAACAAAATAACAACGGGCTCTTT
TCTTCCGGGCTGCCCCACCTTCCTCCCTCACAAAAAAACAATACTA
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