EY744421 | Citrus Genome Database

Overview


Name	EY744421
Unique Name	EY744421
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	996

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: PRP28_ASPOR (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Aspergillus oryzae GN=prp28 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.724e-13
Identity = 32/76 (42.11%), Postives = 48/76 (63.16%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G +VP  + ++ E  L + L   + +  Y +PTP+QR AIPI++  RDL+  A TGSGKTA+F  P++
Sbjct:  351 EDFNISTKGGSVPNPMRSWDESGLPKRLMELVNKVGYKEPTPIQRAAIPIAMQSRDLIGVAVTGSGKTASFLLPLL 426

HSP 2 Score: 32.3426 bits (72), Expect = 1.724e-13
Identity = 23/71 (32.39%), Postives = 33/71 (46.48%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTG-----VRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLI 752
            A++LAPTREL+ QI +   + F  P G     + GG +F    +S       L+ G E     P R +  I
Sbjct:  448 AIVLAPTRELAQQIEI-EAKKFTEPLGFNVVSIVGGHSFEEQAYS-------LRNGAEIIIATPGRLVDCI 510

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: PRP28_ASPCL (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Aspergillus clavatus GN=prp28 PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.724e-13
Identity = 33/76 (43.42%), Postives = 48/76 (63.16%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G +VP  + ++ E  L + L   + R  Y +PTP+QR AIPI++  RDL+  A TGSGKTA+F  P++
Sbjct:  350 EDFNISTKGGSVPNPMRSWEESGLPKRLLELVDRVGYKEPTPIQRAAIPIALQSRDLIGVAVTGSGKTASFLLPLL 425

HSP 2 Score: 31.5722 bits (70), Expect = 1.724e-13
Identity = 22/66 (33.33%), Postives = 29/66 (43.94%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLI 752
            A++LAPTREL+ QI +   + F  P G    V      HS       L+ G E     P R +  I
Sbjct:  447 AIVLAPTRELAQQIEI-EAKKFTQPLGF--NVVSIVGGHSLEEQAYSLRNGAEIIIATPGRLVDCI 509

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: PRP28_ASPTN (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=prp28 PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.725e-13
Identity = 35/76 (46.05%), Postives = 47/76 (61.84%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G +VP  + ++AE  L   L   + R  Y  PTP+QR AIPI++  RDL+  A TGSGKTAAF  P++
Sbjct:  327 EDFNIATKGGSVPNPMRSWAESGLPSRLLDLVHRVGYKDPTPIQRAAIPIAMQSRDLIGVAVTGSGKTAAFLLPLL 402

HSP 2 Score: 30.0314 bits (66), Expect = 1.725e-13
Identity = 22/66 (33.33%), Postives = 29/66 (43.94%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLI 752
            A++LAPTREL+ QI +   + F  P G    V      HS       L+ G E     P R +  I
Sbjct:  424 AIVLAPTRELAQQIEI-EAKKFTIPLGFT--VVSIVGGHSLEEQAYSLRNGAEIIIATPGRLVDCI 486

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: RRP3_LODEL (ATP-dependent rRNA helicase RRP3 OS=Lodderomyces elongisporus GN=RRP3 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.744e-13
Identity = 34/70 (48.57%), Postives = 48/70 (68.57%), Query Frame = 1

Query:  280 TSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            TS  + PP+V +F E DL   L  +I+  KY +PTP+Q  AIP ++ G+D++  A+TGSGKTAAF  PI+
Sbjct:   88 TSSSSSPPSVQSFTEFDLVPELLESIQSLKYTQPTPIQAAAIPHALQGKDIVGIAETGSGKTAAFAIPIL 157

HSP 2 Score: 25.0238 bits (53), Expect = 1.744e-13
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  555 ALILAPTRELSSQI 596
            AL+LAPTREL+ QI
Sbjct:  169 ALVLAPTRELAFQI 182

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: PRP28_MAGGR (Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Magnaporthe grisea GN=PRP28 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.920e-13
Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 1

Query:  265 DIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGSRTVYPSWLLFLPPQE 579
            ++ + T G N+P  +  + E +L   L   I++  Y +PTPVQR AIPI++  RDL+  ++TGSGKTAAF  P++S I  E        ++T  P  L+  P +E
Sbjct:  242 NLEIVTKGNNIPNPMRFWEESNLPHVLKDTIKQVGYTEPTPVQRAAIPIALQCRDLIGISKTGSGKTAAFVLPMLSYI--EPLPPLNEVTKTEGPYALILAPTRE 344

HSP 2 Score: 26.5646 bits (57), Expect = 2.920e-13
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 3

Query:  555 ALILAPTRELSSQI 596
            ALILAPTREL++QI
Sbjct:  336 ALILAPTRELATQI 349

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: RH41_ARATH (DEAD-box ATP-dependent RNA helicase 41 OS=Arabidopsis thaliana GN=RH41 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.942e-13
Identity = 38/76 (50.00%), Postives = 46/76 (60.53%), Query Frame = 1

Query:  265 DIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIIS 492
            DI V+  G  VPP V TF    L   L LN+    Y  PTP+Q  AIP ++ G+ L+A A TGSGKTA+F  PIIS
Sbjct:   95 DIHVQGQGSAVPPPVLTFTSCGLPPKLLLNLETAGYDFPTPIQMQAIPAALTGKSLLASADTGSGKTASFLVPIIS 170

HSP 2 Score: 24.6386 bits (52), Expect = 2.942e-13
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 3

Query:  552 LALILAPTRELSSQI 596
            LA++LAPTREL  Q+
Sbjct:  188 LAMVLAPTRELCVQV 202

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX46_DICDI (ATP-dependent RNA helicase ddx46 OS=Dictyostelium discoideum GN=helB1 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 4.861e-13
Identity = 33/99 (33.33%), Postives = 56/99 (56.57%), Query Frame = 1

Query:  211 EQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMRE 507
            E PV          D   ++ V+ +G++ P  + ++A+  L E ++L +++ +Y KPT +Q   IP  + GRDL+  A+TGSGKT AF  P+   I+ +
Sbjct:  476 EVPVLANMTETEVLDFRSELGVKITGKDCPKPIQSWAQAGLTEKVHLLLKKFQYEKPTSIQAQTIPAIMNGRDLIGIARTGSGKTLAFLLPMFRHILAQ 574

HSP 2 Score: 30.0314 bits (66), Expect = 4.861e-13
Identity = 15/31 (48.39%), Postives = 22/31 (70.97%), Query Frame = 3

Query:  549 LLALILAPTRELSSQIHV*SPRNFPNPTGVR 641
            ++ALI++PTREL+ QIHV   + F    G+R
Sbjct:  583 MIALIMSPTRELALQIHV-ECKKFSKVLGLR 612

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX23_PONAB (Probable ATP-dependent RNA helicase DDX23 OS=Pongo abelii GN=DDX23 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.901e-13
Identity = 31/76 (40.79%), Postives = 47/76 (61.84%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G  +P  + ++ +  L   +   I +C Y +PTP+QR AIPI +  RD++  A+TGSGKTAAF  P++
Sbjct:  375 EDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL 450

HSP 2 Score: 27.7202 bits (60), Expect = 4.901e-13
Identity = 15/29 (51.72%), Postives = 18/29 (62.07%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVR 641
            A+ILAPTREL+ QI       F  P G+R
Sbjct:  471 AIILAPTRELAQQIEE-ETIKFGKPLGIR 498

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX23_HUMAN (Probable ATP-dependent RNA helicase DDX23 OS=Homo sapiens GN=DDX23 PE=1 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 4.901e-13
Identity = 31/76 (40.79%), Postives = 47/76 (61.84%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G  +P  + ++ +  L   +   I +C Y +PTP+QR AIPI +  RD++  A+TGSGKTAAF  P++
Sbjct:  375 EDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL 450

HSP 2 Score: 27.7202 bits (60), Expect = 4.901e-13
Identity = 15/29 (51.72%), Postives = 18/29 (62.07%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVR 641
            A+ILAPTREL+ QI       F  P G+R
Sbjct:  471 AIILAPTRELAQQIEE-ETIKFGKPLGIR 498

[Close]

BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: PRP28_COCIM (Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Coccidioides immitis GN=PRP28 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.902e-13
Identity = 32/76 (42.11%), Postives = 47/76 (61.84%), Query Frame = 1

Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPII 489
            ED  + T G ++P  + ++ E  L + L   I +  Y  P+P+QR AIPI++  RDL+  A TGSGKTAAF  P++
Sbjct:  361 EDFNISTKGGSIPNPMRSWGESGLPKRLLEIIDKVGYKDPSPIQRAAIPIALQNRDLIGVAVTGSGKTAAFLLPLL 436

HSP 2 Score: 31.9574 bits (71), Expect = 4.902e-13
Identity = 25/66 (37.88%), Postives = 29/66 (43.94%), Query Frame = 3

Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLI 752
            A+ILAPTREL+ QI     R F NP G    V      HS       L+ G E     P R +  I
Sbjct:  458 AIILAPTRELAQQIEN-EARKFCNPLGF--NVVSIVGGHSLEEQSFSLRNGAEIIIATPGRLVDCI 520

The following BLAST results are available for this feature:

BLAST of EY744421 vs. ExPASy Swiss-Prot

BLAST of EY744421 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
PRP28_ASPOR	1.724e-13	42.11	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]
PRP28_ASPCL	1.724e-13	43.42	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]
PRP28_ASPTN	1.725e-13	46.05	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]
RRP3_LODEL	1.744e-13	48.57	ATP-dependent rRNA helicase RRP3 OS=Lodderomyces e...	[more]
PRP28_MAGGR	2.920e-13	38.10	Pre-mRNA-splicing ATP-dependent RNA helicase PRP28...	[more]
RH41_ARATH	2.942e-13	50.00	DEAD-box ATP-dependent RNA helicase 41 OS=Arabidop...	[more]
DDX46_DICDI	4.861e-13	33.33	ATP-dependent RNA helicase ddx46 OS=Dictyostelium ...	[more]
DDX23_PONAB	4.901e-13	40.79	Probable ATP-dependent RNA helicase DDX23 OS=Pongo...	[more]
DDX23_HUMAN	4.901e-13	40.79	Probable ATP-dependent RNA helicase DDX23 OS=Homo ...	[more]
PRP28_COCIM	4.902e-13	42.11	Pre-mRNA-splicing ATP-dependent RNA helicase PRP28...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C5-003-008-D10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744421 ID=EY744421; Name=EY744421; organism=Citrus sinensis; type=EST; length=996bp
CCACCAACCTCTTCTGAACCTCCTGCTTCGTCACGAGAATCTACTGAACC
TGCCAGTGGCCCCGGCTGGGGCAGTGGTTCGAGGCCCGATTTTGGGCGTG
GTCAGGGTTACGGCTCTGGTGGTAGAAGTGGCGGTGGTTGGAATAATAGA
AGTGGGGGTTGGGACCGAAGGGAACGTGAAGTGAACCCGTTTGGTGATGA
TGTTGGTGCAGAGCAGCCGGTTGCTGAAGAAGAAAACACGGGTATTAATT
TTGATGCTTATGAGGATATTCCAGTGGAGACAAGTGGAGAAAATGTGCCA
CCGGCAGTTAATACATTTGCAGAAATTGATTTGGGGGAAGCTTTGAATCT
TAATATTAGGAGGTGCAAGTATGTCAAACCGACGCCTGTTCAGCGTCATG
CGATTCCTATATCGATTGGTGGGCGAGATTTAATGGCCTGTGCTCAGACA
GGGTCGGGGAAGACAGCTGCCTTTTGTTTTCCAATTATTAGTGGGATTAT
GCGTGAGCAGTACGTACAGAGACCACGTGGATCGCGGACTGTTTATCCCT
CCTGGCTCTTATTCTTGCCCCCACAAGAGAACTCTCGTCTCAGATCCATG
TTTGAAGCCCCAGAAATTTTCCTAATCCAACTGGGGTCAGGGGGGGGGTT
GCTTTCCGAAGGAACCACCATTCAACCCACCATTTGCGAAAACTTTTAAA
AAAAGGGGTTGAATATTCCTTTGGCCAACCCCCGCGAAAAATGGGTTTGA
TTCGCGTTGAAAAAACCTAAAATTTCTCTTTGCAAAAAGATTAGAATCCT
TTACCGCTTCCAATGAAGGGCAGACGCGGAGGCTGGATATTGGGGGTTTT
TGGCCCCCCGCAACGCCAAACAATATTTGCTGCCCCACCAACGGGTCTTA
TGCCCTCCCCCCCACCTGTGGGTTACAAACAAAATAACAACGGGCTCTTT
TCTTCCGGGCTGCCCCACCTTCCTCCCTCACAAAAAAACAATACTA