EY744421

Overview
NameEY744421
Unique NameEY744421
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length996
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: RRP3_DEBHA (ATP-dependent rRNA helicase RRP3 OS=Debaryomyces hansenii GN=RRP3 PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 6.457e-13
Identity = 40/101 (39.60%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  208 AEQPVAEEENTGINFDAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQ 510
            A++  A+EEN   NFD   D   E           +F E+ L   L   I++ K+ KPTP+Q  AIP ++ G+D++  AQTGSGKTAAF  PI+  +   Q
Sbjct:   38 AQEEPAKEENVAENFDTVVDPTAELK-------FKSFNELKLIPELLEAIQQMKFTKPTPIQSEAIPHALEGKDIIGLAQTGSGKTAAFAIPILQALWEAQ 131

HSP 2 Score: 23.483 bits (49), Expect = 6.457e-13
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  558 LILAPTRELSSQI 596
            L+LAPTREL+ QI
Sbjct:  137 LVLAPTRELAYQI 149
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX42_PONAB (ATP-dependent RNA helicase DDX42 OS=Pongo abelii GN=DDX42 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.238e-13
Identity = 32/89 (35.96%), Postives = 54/89 (60.67%), Query Frame = 1 
Query:  253 DAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQ 519
            D    + +  SG   P   ++FA     E L   IR+ +Y +PTP+Q   +P+++ GRD++  A+TGSGKTAAF +P++  IM ++ ++
Sbjct:  234 DLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELE 322

HSP 2 Score: 26.1794 bits (56), Expect = 8.238e-13
Identity = 20/72 (27.78%), Postives = 32/72 (44.44%), Query Frame = 3 
Query:  552 LALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLIRVEKT 767
            +A+I+ PTREL  QIH    + F     +R    +     S     K L++G E     P R +  ++ + T
Sbjct:  328 IAVIVCPTRELCQQIHA-EGKRFGKAYNLRSVAVY--GGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKAT 396
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX23_DICDI (ATP-dependent RNA helicase ddx23 OS=Dictyostelium discoideum GN=helB2 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.263e-13
Identity = 33/82 (40.24%), Postives = 50/82 (60.98%), Query Frame = 1 
Query:  262 EDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMRE 507
            ED  + T G   P  + T+ E +L   +   IR+  Y KP+P+Q  +IPIS+ GRD++  A+TGSGKT AF  P++  I ++
Sbjct:  397 EDFNISTKGGIAPNPIRTWQESNLPREILEAIRQLGYEKPSPIQMQSIPISLTGRDILGIAETGSGKTCAFVIPMLIYISKQ 478

HSP 2 Score: 26.5646 bits (57), Expect = 8.263e-13
Identity = 15/29 (51.72%), Postives = 17/29 (58.62%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIHV*SPRNFPNPTGVR 641
            AL++APTREL  QI     RNF    G R
Sbjct:  492 ALVMAPTRELVQQIEK-ETRNFAQHFGFR 519
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX42_HUMAN (ATP-dependent RNA helicase DDX42 OS=Homo sapiens GN=DDX42 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.070e-12
Identity = 32/89 (35.96%), Postives = 54/89 (60.67%), Query Frame = 1 
Query:  253 DAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQ 519
            D    + +  SG   P   ++FA     E L   IR+ +Y +PTP+Q   +P+++ GRD++  A+TGSGKTAAF +P++  IM ++ ++
Sbjct:  234 DLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELE 322

HSP 2 Score: 25.7942 bits (55), Expect = 1.070e-12
Identity = 20/72 (27.78%), Postives = 32/72 (44.44%), Query Frame = 3 
Query:  552 LALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLIRVEKT 767
            +A+I+ PTREL  QIH    + F     +R    +     S     K L++G E     P R +  ++ + T
Sbjct:  328 IAVIVCPTRELCQQIHA-ECKRFGKAYNLRSVAVY--GGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKAT 396
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX42_MOUSE (ATP-dependent RNA helicase DDX42 OS=Mus musculus GN=Ddx42 PE=1 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 1.070e-12
Identity = 32/89 (35.96%), Postives = 54/89 (60.67%), Query Frame = 1 
Query:  253 DAYEDIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQ 519
            D    + +  SG   P   ++FA     E L   IR+ +Y +PTP+Q   +P+++ GRD++  A+TGSGKTAAF +P++  IM ++ ++
Sbjct:  234 DLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELE 322

HSP 2 Score: 25.7942 bits (55), Expect = 1.070e-12
Identity = 20/72 (27.78%), Postives = 32/72 (44.44%), Query Frame = 3 
Query:  552 LALILAPTRELSSQIHV*SPRNFPNPTGVRGGVAFRRNHHSTHHLRKLLKKGVEYSFGQPPRKMGLIRVEKT 767
            +A+I+ PTREL  QIH    + F     +R    +     S     K L++G E     P R +  ++ + T
Sbjct:  328 IAVIVCPTRELCQQIHA-ECKRFGKAYNLRSVAVY--GGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKAT 396
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: RRP3_PICGU (ATP-dependent rRNA helicase RRP3 OS=Pichia guilliermondii GN=RRP3 PE=3 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 1.414e-12
Identity = 37/84 (44.05%), Postives = 51/84 (60.71%), Query Frame = 1 
Query:  274 VETSGE-----NVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQ 510
            VET+ E     N     +TF+E++L   L   I + KY KPTP+Q  AIP ++ G+D++  AQTGSGKTAAF  PI+  +   Q
Sbjct:   47 VETTSEASQDVNSEQQFHTFSELNLVPELMEAIEKLKYTKPTPIQSGAIPHALEGKDIIGLAQTGSGKTAAFAIPILQSLWEAQ 130

HSP 2 Score: 25.0238 bits (53), Expect = 1.414e-12
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  555 ALILAPTRELSSQI 596
            AL+LAPTREL+ QI
Sbjct:  135 ALVLAPTRELAYQI 148
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DDX42_CHICK (ATP-dependent RNA helicase DDX42 OS=Gallus gallus GN=DDX42 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.040e-12
Identity = 31/82 (37.80%), Postives = 52/82 (63.41%), Query Frame = 1 
Query:  274 VETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQ 519
            +  SG   P   ++FA     E L   IR+ +Y +PTP+Q   +P+++ GRD++  A+TGSGKTAAF +P++  IM ++ ++
Sbjct:  241 LRVSGAAPPRPGSSFARFGFDEQLMHQIRKSEYTQPTPIQCQGVPVAMSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELE 322

HSP 2 Score: 25.409 bits (54), Expect = 3.040e-12
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 3 
Query:  552 LALILAPTRELSSQIH 599
            +A+I+ PTREL  QIH
Sbjct:  328 IAVIVCPTRELCQQIH 343
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP9_NEUCR (ATP-dependent RNA helicase dbp-9 OS=Neurospora crassa GN=dbp-9 PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.066e-12
Identity = 34/89 (38.20%), Postives = 56/89 (62.92%), Query Frame = 1 
Query:  313 TFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGSRTVYPSWLLFLPPQE 579
            TF+++ L   L   + +  + KPT VQR AIP+++ G+D++  A+TGSGKTAA+  P++SGI++     R     T + S L+ +P +E
Sbjct:   96 TFSDLGLDPRLVQAVAKQSFEKPTLVQRKAIPLALAGQDVLCKAKTGSGKTAAYVLPVLSGILK-----RKATDPTPFTSALILVPTRE 179

HSP 2 Score: 27.335 bits (59), Expect = 3.066e-12
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIH 599
            ALIL PTREL+ Q+H
Sbjct:  171 ALILVPTRELADQVH 185
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP9_AJECN (ATP-dependent RNA helicase DBP9 OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=DBP9 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.073e-12
Identity = 28/80 (35.00%), Postives = 55/80 (68.75%), Query Frame = 1 
Query:  271 PVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQ 510
            P +++G+++    ++F  + L   L   + + K+ KPTP+Q  AIP+++ G+D++A A+TGSGKTAA+  P++  I++++
Sbjct:   13 PEDSAGKSI--TAHSFETLKLDPRLLQALTQQKFTKPTPIQAEAIPLALDGKDVLARAKTGSGKTAAYLLPVLQSILQQK 90

HSP 2 Score: 28.8758 bits (63), Expect = 3.073e-12
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  546 SLLALILAPTRELSSQIH 599
            S+ ALIL PTREL+ Q+H
Sbjct:   98 SISALILVPTRELAEQVH 115
[Close]
BLAST of EY744421 vs. ExPASy Swiss-Prot
Match: DBP9_BOTFB (ATP-dependent RNA helicase dbp9 OS=Botryotinia fuckeliana (strain B05.10) GN=dbp9 PE=3 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 3.075e-12
Identity = 40/105 (38.10%), Postives = 59/105 (56.19%), Query Frame = 1 
Query:  265 DIPVETSGENVPPAVNTFAEIDLGEALNLNIRRCKYVKPTPVQRHAIPISIGGRDLMACAQTGSGKTAAFCFPIISGIMREQYVQRPRGSRTVYPSWLLFLPPQE 579
            D+PV T G +      TFA + L   L   I +  +  PT VQ  AIP+++ GRD++A A+TGSGKTAA+  PI+  I++     R   S T   + L+ +P +E
Sbjct:    9 DVPVPTEGSDAIKENVTFASLGLDARLLQGIAKQNFQSPTLVQSKAIPLTLEGRDILARAKTGSGKTAAYLLPILHSILK-----RKELSSTQCTTALILVPTRE 108

HSP 2 Score: 25.0238 bits (53), Expect = 3.075e-12
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  555 ALILAPTRELSSQIH 599
            ALIL PTREL+ Q++
Sbjct:  100 ALILVPTRELADQVY 114
The following BLAST results are available for this feature:
BLAST of EY744421 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133
Match NameE-valueIdentityDescription
RRP3_DEBHA6.457e-1339.60ATP-dependent rRNA helicase RRP3 OS=Debaryomyces h... [more]
DDX42_PONAB8.238e-1335.96ATP-dependent RNA helicase DDX42 OS=Pongo abelii G... [more]
DDX23_DICDI8.263e-1340.24ATP-dependent RNA helicase ddx23 OS=Dictyostelium ... [more]
DDX42_HUMAN1.070e-1235.96ATP-dependent RNA helicase DDX42 OS=Homo sapiens G... [more]
DDX42_MOUSE1.070e-1235.96ATP-dependent RNA helicase DDX42 OS=Mus musculus G... [more]
RRP3_PICGU1.414e-1244.05ATP-dependent rRNA helicase RRP3 OS=Pichia guillie... [more]
DDX42_CHICK3.040e-1237.80ATP-dependent RNA helicase DDX42 OS=Gallus gallus ... [more]
DBP9_NEUCR3.066e-1238.20ATP-dependent RNA helicase dbp-9 OS=Neurospora cra... [more]
DBP9_AJECN3.073e-1235.00ATP-dependent RNA helicase DBP9 OS=Ajellomyces cap... [more]
DBP9_BOTFB3.075e-1238.10ATP-dependent RNA helicase dbp9 OS=Botryotinia fuc... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744421 ID=EY744421; Name=EY744421; organism=Citrus sinensis; type=EST; length=996bp
CCACCAACCTCTTCTGAACCTCCTGCTTCGTCACGAGAATCTACTGAACC
TGCCAGTGGCCCCGGCTGGGGCAGTGGTTCGAGGCCCGATTTTGGGCGTG
GTCAGGGTTACGGCTCTGGTGGTAGAAGTGGCGGTGGTTGGAATAATAGA
AGTGGGGGTTGGGACCGAAGGGAACGTGAAGTGAACCCGTTTGGTGATGA
TGTTGGTGCAGAGCAGCCGGTTGCTGAAGAAGAAAACACGGGTATTAATT
TTGATGCTTATGAGGATATTCCAGTGGAGACAAGTGGAGAAAATGTGCCA
CCGGCAGTTAATACATTTGCAGAAATTGATTTGGGGGAAGCTTTGAATCT
TAATATTAGGAGGTGCAAGTATGTCAAACCGACGCCTGTTCAGCGTCATG
CGATTCCTATATCGATTGGTGGGCGAGATTTAATGGCCTGTGCTCAGACA
GGGTCGGGGAAGACAGCTGCCTTTTGTTTTCCAATTATTAGTGGGATTAT
GCGTGAGCAGTACGTACAGAGACCACGTGGATCGCGGACTGTTTATCCCT
CCTGGCTCTTATTCTTGCCCCCACAAGAGAACTCTCGTCTCAGATCCATG
TTTGAAGCCCCAGAAATTTTCCTAATCCAACTGGGGTCAGGGGGGGGGTT
GCTTTCCGAAGGAACCACCATTCAACCCACCATTTGCGAAAACTTTTAAA
AAAAGGGGTTGAATATTCCTTTGGCCAACCCCCGCGAAAAATGGGTTTGA
TTCGCGTTGAAAAAACCTAAAATTTCTCTTTGCAAAAAGATTAGAATCCT
TTACCGCTTCCAATGAAGGGCAGACGCGGAGGCTGGATATTGGGGGTTTT
TGGCCCCCCGCAACGCCAAACAATATTTGCTGCCCCACCAACGGGTCTTA
TGCCCTCCCCCCCACCTGTGGGTTACAAACAAAATAACAACGGGCTCTTT
TCTTCCGGGCTGCCCCACCTTCCTCCCTCACAAAAAAACAATACTA
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