EY744418 | Citrus Genome Database

Overview


Name	EY744418
Unique Name	EY744418
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1040

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.852e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 82.4185 bits (202), Expect = 4.720e-15
Identity = 43/77 (55.84%), Postives = 54/77 (70.13%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWG 566
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T AD       S LHLVL L  G
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLG-GSVLHLVLALRGG 76

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 2.418e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 3

Query:  114 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 338
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 82.0333 bits (201), Expect = 6.164e-15
Identity = 45/78 (57.69%), Postives = 56/78 (71.79%), Query Frame = 3

Query:  342 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGGLL 575
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  D       S LHLVL L  GGLL
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYA-IEGGSVLHLVLALR-GGLL 78

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.674e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 1.794e-14
Identity = 40/78 (51.28%), Postives = 55/78 (70.51%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGG 569
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T AD       S LHLVL L  GG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILG-GSVLHLVLALRGGG 77

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.674e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 1.794e-14
Identity = 40/78 (51.28%), Postives = 55/78 (70.51%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGG 569
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T AD       S LHLVL L  GG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILG-GSVLHLVLALRGGG 77

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.674e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 1.794e-14
Identity = 40/78 (51.28%), Postives = 55/78 (70.51%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGG 569
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T AD       S LHLVL L  GG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILG-GSVLHLVLALRGGG 77

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.674e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 1.794e-14
Identity = 40/78 (51.28%), Postives = 55/78 (70.51%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGG 569
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T AD       S LHLVL L  GG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILG-GSVLHLVLALRGGG 77

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.674e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 1.794e-14
Identity = 40/78 (51.28%), Postives = 55/78 (70.51%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWGG 569
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T AD       S LHLVL L  GG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILG-GSVLHLVLALRGGG 77

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 3.492e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 76.6406 bits (187), Expect = 2.590e-13
Identity = 37/77 (48.05%), Postives = 53/77 (68.83%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWG 566
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  D       S LHLVL L  G
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDY-KIEAGSVLHLVLALRGG 76

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.264e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 335
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 73.559 bits (179), Expect = 2.192e-12
Identity = 39/77 (50.65%), Postives = 53/77 (68.83%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWG 566
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T A   +  + S LHLVL L  G
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLT-AKHYNLERGSVLHLVLALRGG 76

BLAST of EY744418 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.956e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 3

Query:  108 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 338
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 72.4034 bits (176), Expect = 4.884e-12
Identity = 37/77 (48.05%), Postives = 51/77 (66.23%), Query Frame = 3

Query:  336 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADLPHFSKESTLHLVLRLPWG 566
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++       S LHLVL L  G
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEY-SIEGGSVLHLVLALRGG 76

The following BLAST results are available for this feature:

BLAST of EY744418 vs. ExPASy Swiss-Prot

BLAST of EY744418 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 95


Match Name	E-value	Identity	Description
NEDD8_CAEEL	1.852e-19	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	2.418e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	2.674e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	2.674e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	2.674e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	2.674e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	2.674e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	3.492e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	2.264e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.956e-17	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-008-D07-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744418 ID=EY744418; Name=EY744418; organism=Citrus sinensis; type=EST; length=1040bp
CCCGCGTCCGCTTCACCGGAAAGCAGCTCGAGGACGGGCGCACCTTGGCG
GATTACAACATCCAGAAGGAGTCGACTCTCCACCTCGTCCTCCGTCTCCG
CGGCGGCATGCAGATCTTCGTCAAGACTCTGACCGGGAAGACGATCACCC
TGGAGGTGGAGAGCTCCGACACGATCGACAACGTGAAGGCGAAGATCCAG
GACAAGGAAGGGATCCCCCCGGACCAGCAGAGGCTCATCTTCGCCGGCAA
GCAGCTGGAGGACGGCCGCACCCTGGCCGATTATAACATCCAGAAGGAAT
CCACGCTCCACCTTGTGTTGCGCCTGAGGGGAGGAATGCAGATCTTCGTC
AAGACGTTGACCGGGAAGACGATCACCCTGGAGGTGGAGAGCTCCGACAC
GATCGACAACGTGAAGGCCAAGATCCAGGACAAAGAGGGGATTCCTCCGG
ACCAGCAGAGGCTCATCTTCGCCGGCAAGCAGCTCGAGGATGGCCGGACT
CTCGCCGATTTACCACATTTCTCAAAGGAGTCCACTCTCCACCTTGTCCT
CCGTCTCCCGTGGGGGGGACTTCTAATAATTTAAATGGTGGATGGGTCCG
CTTTTGATTGGATGGCGCCTATAATACTTAATTAAGTTTGGATCGGGGGG
CTTTGCTTTTAATTTTTTTTGGTAATTTCAAATGGGGTAGGGGGCCCACC
CGCTTGGGGGCCTTAATTTCCTCCTTTCTATGGGGAAATAAACAAATATA
AGCCACTAGTTTTTTGAACCAACCACCAAACGAAAGCATGAGCACACAGA
AAAACAACCAATGCATAATATTCTTCAGCGGCCAACCCCGCGCTCCACCC
GCCGGCACTCCCCCACCCCTCCTCCCCTACTTACCCCCCCGGGGGGGCCG
CCGCCCTCTCTACCACCCCCCTGTCTCTTTACATATTCTCCCGGGACGGT
CGGGACAAGTATTAACATTTCTCCTTTCTTTAATTAGGTTACCACCACCT
CAGATCAGCATTTTTTTTCCTTTCCCCCGGGCGCCCGCCG