EY744212 | Citrus Genome Database

Overview


Name	EY744212
Unique Name	EY744212
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	889

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 3.060e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 2

Query:   56 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 142.51 bits (358), Expect = 3.060e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 93.2041 bits (230), Expect = 2.129e-18
Identity = 51/76 (67.11%), Postives = 55/76 (72.37%), Query Frame = 2

Query:  512 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKH*KMEPHLQNTTSKKNQPFLWAPRPRGG 739
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGK  +    L +   +K        R RGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 9.844e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 2

Query:   56 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 137.502 bits (345), Expect = 9.844e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 88.1965 bits (217), Expect = 6.849e-17
Identity = 49/76 (64.47%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  512 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKH*KMEPHLQNTTSKKNQPFLWAPRPRGG 739
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA K  +    L +   +K        R RGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.056e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 2

Query:   56 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 125.176 bits (313), Expect = 5.056e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 79.7221 bits (195), Expect = 2.436e-14
Identity = 44/83 (53.01%), Postives = 51/83 (61.45%), Query Frame = 2

Query:  512 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKH*KMEPHLQNTTSKKNQPFLWAPRPRGGKAKFLGK 760
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGK  +    L +   +K        R RGG     GK
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGGNGLRKGK 83

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 3.277e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 2

Query:   56 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 122.479 bits (306), Expect = 3.277e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 75.485 bits (184), Expect = 4.594e-13
Identity = 39/76 (51.32%), Postives = 50/76 (65.79%), Query Frame = 2

Query:  512 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKH*KMEPHLQNTTSKKNQPFLWAPRPRGG 739
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGK  +    + +   +K        R RGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 1.245e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 2

Query:   89 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 277
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 120.553 bits (301), Expect = 1.245e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 2

Query:  317 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 505
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 72.0182 bits (175), Expect = 8.578e-15
Identity = 35/37 (94.59%), Postives = 36/37 (97.30%), Query Frame = 2

Query:  545 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGK 655
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGK
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGK 37

HSP 4 Score: 29.6462 bits (65), Expect = 8.578e-15
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = 1

Query:  658 LKDGTTLAEHNIQKEPTLPL 717
            L+DG TL+++NIQKE TL L
Sbjct:   39 LEDGRTLSDYNIQKESTLHL 58

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.971e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 2

Query:   62 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 99.3673 bits (246), Expect = 2.971e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:   62 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:   62 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:   62 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 511
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY744212 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 2

Query:   56 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 292
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 98.9821 bits (245), Expect = 3.880e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 520
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

The following BLAST results are available for this feature:

BLAST of EY744212 vs. ExPASy Swiss-Prot

BLAST of EY744212 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
UBIQ_LEIMA	3.060e-33	93.42	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	9.844e-32	90.79	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	5.056e-28	80.26	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIL_NPVAC	3.277e-27	76.32	Ubiquitin-like protein OS=Autographa californica n...	[more]
UBIQ_LUMTE	1.245e-26	95.24	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
RUB1_DESAN	2.971e-20	64.86	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	3.880e-20	63.51	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]
RUB1_ORYSJ	3.880e-20	63.51	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB1_ARATH	3.880e-20	63.51	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DROME	3.880e-20	58.23	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-001-B05-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744212 ID=EY744212; Name=EY744212; organism=Citrus sinensis; type=EST; length=889bp
CCACGCGTCCGACCTTTAATTCGATAAAAGGCCTCCTTCTGTTCTCTCTG
TCAAGATGCAAATCTTTGTGAAAACTCTCACCGGCAAGACAATAACCCTT
GAGGTTGAAAGCTCCGACACCATTGATAATGTAAAAGCCAAGATCCAGGA
CAAGGAAGGGATCCCACCAGATCAGCAGAGGCTTATCTTTGCTGGTAAAC
AGCTCGAGGATGGTCGCACACTTGCTGACTATAATATCCAGAAGGAATCA
ACCCTCCACTTGGTCCTTCGACTTAGGGGTGGTATGCAGATCTTTGTAAA
GACCCTCACTGGCAAGACGATTACTCTTGAGGTGGAAAGCTCCGACACCA
TTGACAATGTGAAAGCCAAGATTCAAGACAAGGAAGGGATCCCACCAGAC
CAACAGAGACTTATCTTTGCCGGTAAACAGCTCGAGGATGGTCGCACACT
TGCTGATTATAACATCCAGAAGGAGTCAACCCTCCACTTGGTCCTCCGTC
TCCGTGGTGGTATGCAGATCTTTGTAAAGACCCTCACTGGAAAGACTATC
ACCCTTGAGGTGGAAAGCTCCGACACCATTGACAATGTCAAAGCCAAAAT
TCAGGATAAGGAAGGGATCCCACCAGACCAGCAAAGGCTCATCTTTGCTG
GGAAACACTAAAAGATGGAACCACACTTGCAGAACACAACATCCAAAAAG
AACCAACCCTTCCTTTGGGCCCCCCGTCCCAGGGGGGGGAAGGCAAAATT
TTTGGGGAAAACCCCGACCCGGGAAAAAAAAAAACTTTTGGAAGTTGGAA
AGCTCCGGAAACCAATTCAAAAAGGGTCAAGGGGCCAAAAATTCCCGGAA
AAGGGGGGGGGCTTCCCCCCCCTGGTTCAAAAAAAAGGG