EY659597

Overview
NameEY659597
Unique NameEY659597
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length888
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: AQPZ_ECOL6 (Aquaporin Z OS=Escherichia coli O6 GN=aqpZ PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.517e-16
Identity = 56/171 (32.75%), Postives = 85/171 (49.71%), Query Frame = 2
Query:  248 NISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTNGQT-----TSAFALS-------SGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALV--SWSWDNHWVYWVGPLIGGGLAGIVY 718
            +ISGGH NPAVT G + GG       + Y IAQ++G  VA  LL  + +G+T      S FA +        G    +A+V E+V++ G +  ++       K +    APIAIG  +    L        S+NPA S   A+    W+ +  W +WV P++GG + G++Y
Sbjct:   56 HISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGAT---DKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIY 223          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: AQPZ_ECO57 (Aquaporin Z OS=Escherichia coli O157:H7 GN=aqpZ PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.517e-16
Identity = 56/171 (32.75%), Postives = 85/171 (49.71%), Query Frame = 2
Query:  248 NISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTNGQT-----TSAFALS-------SGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALV--SWSWDNHWVYWVGPLIGGGLAGIVY 718
            +ISGGH NPAVT G + GG       + Y IAQ++G  VA  LL  + +G+T      S FA +        G    +A+V E+V++ G +  ++       K +    APIAIG  +    L        S+NPA S   A+    W+ +  W +WV P++GG + G++Y
Sbjct:   56 HISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGAT---DKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIY 223          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: AQPZ_SHEON (Aquaporin Z OS=Shewanella oneidensis GN=aqpZ PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.982e-16
Identity = 60/176 (34.09%), Postives = 88/176 (50.00%), Query Frame = 2
Query:  248 NISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTNGQTTSAFALSSGVGA-------------WNAVVFEIVMT-FGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALV--SWSWDNHWVYWVGPLIGGGLAGIVYEFF 727
            +ISG H+NPAV+FG + GG       + Y IAQ+ G      +L  + +GQ    F+L++G  +              + ++ EIVMT F L+  + +T     KG     APIAIG  +    L        S+NPA S GPAL    W+    W++W  P+IG  LAG++Y +F
Sbjct:   56 HISGCHLNPAVSFGLWAGGRFPTSELLPYIIAQVAGGIAGAGVLYLIASGQ--EGFSLAAGFASNGFGEHSPGGYSMISVMICEIVMTLFFLLVILGSTDERAPKG----FAPIAIGLCLTLIHLISIPISNTSVNPARSTGPALFVGDWAVSQLWLFWAAPIIGAILAGVIYRYF 225          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: NIP12_ARATH (Aquaporin NIP1-2 OS=Arabidopsis thaliana GN=NIP1-2 PE=1 SV=2)

HSP 1 Score: 85.5001 bits (210), Expect = 4.415e-16
Identity = 63/222 (28.38%), Postives = 97/222 (43.69%), Query Frame = 2
Query:   74 LRAALAEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXXNISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKF-------VTNGQTTSAFALSSGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWSWDNHWVYWVGPLIGGGLAGIVY 718
            L+  +AE + T   +FAG  + +A N         P                      +ISG H NPAVT      G   L +   Y I+Q++GST+A   L+        V +G+               + V E ++TF L++ +   A D +  ++G +A +A+G  V  N++  G   GASMNP  S GPA+V   +   W+Y V P++G      VY
Sbjct:   51 LQKLMAEVLGTYFLIFAG-CAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLG----HISGAHFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTLPSGSNLQSFVIEFIITFYLMFVISGVATDNR--AIGELAGLAVGSTVLLNVIIAGPVSGASMNPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVY 265          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: AQPM_METTH (Aquaporin aqpM OS=Methanobacterium thermoautotrophicum GN=aqpM PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.766e-16
Identity = 75/243 (30.86%), Postives = 106/243 (43.62%), Query Frame = 2
Query:   77 RAALAEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXX-------------NISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVA------CLLLKFVTNGQTTSAFALSSGVGAWNAVVFEIVMTFGLVYTVYATALDPK--KGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAL--VSWSWDNHW----VYWVGPLIGGGLAGIVYEF 724
            +  +AEFI T   VF G G+  A   +  +G   P+                                  NISG H+NPAVT G +          + Y IAQLLG+  A      C  +   T G    A A   G+G W A++ E V TF L+ T+   A+D +  KG  G I    IG  V   I   G   G+S+NPA +FGP L  + ++  N W    +Y +GP++G  LA + Y++
Sbjct:    6 KRCIAEFIGTFFLVFFGAGAA-AITLMIASGGTAPNPFNIGIGLLGGLGDWVAIGLAFGFAIAASIYALGNISGCHINPAVTIGLWSVKKFPGRDVVPYIIAQLLGAAFASFIFLQCAGITAATIGGL-GATAPFPGIGYWQAMLAETVGTFLLMITIMGIAVDERAPKGFAGII----IGLTVAGIITTIGNITGSSLNPARTFGPYLNDMVFAGTNLWNYFPIYVIGPVVGAVLAALTYQY 242          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: NIP31_MAIZE (Aquaporin NIP3-1 OS=Zea mays GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.530e-16
Identity = 63/231 (27.27%), Postives = 102/231 (44.16%), Query Frame = 2
Query:   89 AEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXXNISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLK-----FVTNGQTTSAFALSSGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWSWDNHWVYWVGPLIGGGLAGIVYEFFFINQSHEQLPNTE 766
            AEF+ T I +F    + +   K  + GA +P                      +ISG H+NP++T       +   L+   Y   Q L S  A   LK     F++ G T     +S+      A   E +++F L++ V A A D +  ++G +A IA+G  V  NIL  G   G SMNP  + GPA+ + ++   W+Y + P +G      VY+   +   + + P T+
Sbjct:   78 AEFVGTFILIFFATAAPIVNQK--YGGAISP---FGNAACAGLAVATVILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQALASVCAAFALKGVFHPFLSGGVTVPDATVSTA----QAFFTEFIISFNLLFVVTAVATDTR--AVGELAGIAVGAAVTLNILVAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLLAPTLGALAGASVYKAVKLRDENGETPRTQ 297          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: NIP31_ORYSJ (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 84.3445 bits (207), Expect = 9.835e-16
Identity = 64/231 (27.71%), Postives = 102/231 (44.16%), Query Frame = 2
Query:   89 AEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXXNISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLK-----FVTNGQTTSAFALSSGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWSWDNHWVYWVGPLIGGGLAGIVYEFFFINQSHEQLPNTE 766
            AEF+ T I +F    + +   K  + GA +P                      +ISG H+NP++T       +   L+   Y   Q+LGS  A   LK     F++ G T     +S+      A   E ++TF L++ V A A D +  ++G +A IA+G  V  NIL  G   G SMNP  + GPA+ + ++   W+Y + P +G      VY    +   + + P  +
Sbjct:   87 AEFVGTFILIFFATAAPIVNQK--YGGAISP---FGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFALKGVFHPFLSGGVTVPDPTISTA----QAFFTEFIITFNLLFVVTAVATDTR--AVGELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGETPRPQ 306          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: NIP51_ARATH (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.678e-15
Identity = 65/218 (29.82%), Postives = 95/218 (43.58%), Query Frame = 2
Query:   89 AEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXXNISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTNGQTTSAFALSSGVGAWNAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWSWDNHWVYWVGPLIGGGLAGIVYEFFFINQS 742
            AEF+ T I +F      +   K  ++GA T                       +ISG H+NP++T       +        Y  AQ+  S  A   LK V +   +    + S V    A   E ++TF L++ V A A D +  ++G +A IA+G  V  NIL  G   G SMNP  + GPA+ S ++ + WVY V P +G      VY    +N S
Sbjct:   82 AEFVGTFILIFTATAGPIVNQK--YDGAET---LIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPS-VSLGQAFALEFIITFILLFVVTAVATDTR--AVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDS 291          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: NIP31_ARATH (Aquaporin NIP3-1 OS=Arabidopsis thaliana GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 1.678e-15
Identity = 65/210 (30.95%), Postives = 94/210 (44.76%), Query Frame = 2
Query:   86 LAEFISTLIFVFAGEGSGMAFNKLTHNGANTPSXXXXXXXXXXXXXXXXXXXXXNISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTN-GQTTSAFALSSGVGAW------NAVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWSWDNHWVYWVGPLIG 694
            + EF+ T   +FAG  S +  N+        P                      ++SG H NPAV+            +   Y  AQLLGST+A  +L+ V +      +      VG +       + V E + TF L++ + A A D  K + G+ A IAIG  +  +IL  G   GASMNPA S GPAL+   + + W+Y V P+IG
Sbjct:   46 IGEFVGTFTMIFAG-CSAIVVNETYGKPVTLPGIALVWGLVVTVMIYSIG----HVSGAHFNPAVSIAFASSKKFPFNQVPGYIAAQLLGSTLAAAVLRLVFHLDDDVCSLKGDVYVGTYPSNSNTTSFVMEFIATFNLMFVISAVATD--KRATGSFAGIAIGATIVLDILFSGPISGASMNPARSLGPALIWGCYKDLWLYIVSPVIG 248          
BLAST of EY659597 vs. ExPASy Swiss-Prot
Match: AQPZ_BRAJA (Aquaporin Z OS=Bradyrhizobium japonicum GN=aqpZ PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.678e-15
Identity = 59/173 (34.10%), Postives = 83/173 (47.98%), Query Frame = 2
Query:  248 NISGGHVNPAVTFGAFVGGNISLLRGILYWIAQLLGSTVACLLLKFVTNG----QTTSAFALSSGVGAWN--------AVVFEIVMTFGLVYTVYATALDPKKGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALV--SWSWDNHWVYWVGPLIGGGLAGIVYEF 724
            +ISG H+NPAVT G   GG     + + Y IAQ+ G+ VA  LL  + +G      T  FA S+G  A +          + E+VMT   ++ +               AP+AIG  +    L        S+NPA S GPAL    W+    W++WV PLIGG L G++Y +
Sbjct:   57 HISGCHLNPAVTVGLAAGGRFPAGQILPYVIAQVCGAIVAAELLYIIASGAPGFDVTKGFA-SNGYDAHSPGQYSMMACFLTEVVMTMMFLFIIMGATHGRAPAG---FAPLAIGLALVMIHLVSIPVTNTSVNPARSTGPALFVGGWAMAQLWLFWVAPLIGGALGGVIYRW 225          
The following BLAST results are available for this feature:
BLAST of EY659597 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
AQPZ_ECOL61.517e-1632.75Aquaporin Z OS=Escherichia coli O6 GN=aqpZ PE=3 SV... [more]
AQPZ_ECO571.517e-1632.75Aquaporin Z OS=Escherichia coli O157:H7 GN=aqpZ PE... [more]
AQPZ_SHEON1.982e-1634.09Aquaporin Z OS=Shewanella oneidensis GN=aqpZ PE=3 ... [more]
NIP12_ARATH4.415e-1628.38Aquaporin NIP1-2 OS=Arabidopsis thaliana GN=NIP1-2... [more]
AQPM_METTH5.766e-1630.86Aquaporin aqpM OS=Methanobacterium thermoautotroph... [more]
NIP31_MAIZE7.530e-1627.27Aquaporin NIP3-1 OS=Zea mays GN=NIP3-1 PE=2 SV=1[more]
NIP31_ORYSJ9.835e-1627.71Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica G... [more]
NIP51_ARATH1.678e-1529.82Probable aquaporin NIP5-1 OS=Arabidopsis thaliana ... [more]
NIP31_ARATH1.678e-1530.95Aquaporin NIP3-1 OS=Arabidopsis thaliana GN=NIP3-1... [more]
AQPZ_BRAJA1.678e-1534.10Aquaporin Z OS=Bradyrhizobium japonicum GN=aqpZ PE... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-011-H11-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659597 ID=EY659597; Name=EY659597; organism=Citrus sinensis; type=EST; length=888bp
ATCGTTTGATCTCCATCATGCCGATCAGAACATTGCCGTTGGCCATCCCC
GTGAGGCGACTCATCCAGATGCCCTAAGGGCAGCCCTAGCAGAGTTCATC
TCAACTCTAATATTTGTCTTTGCCGGTGAAGGCTCGGGCATGGCCTTCAA
CAAGCTCACTCACAACGGCGCCAACACCCCCTCCGGCCTCGTCGCCGCCT
CTGTTGCTCACGCTTTCGCCCTTTTCGTTGCTGTCGCCGTTGGCGCCAAC
ATTTCCGGTGGCCACGTTAATCCTGCTGTCACCTTTGGCGCCTTCGTTGG
AGGTAACATCTCCCTTCTACGAGGCATCCTCTACTGGATCGCCCAGCTTC
TTGGATCCACCGTCGCATGCTTGCTTCTTAAGTTCGTTACCAACGGCCAG
ACCACATCTGCTTTTGCTCTCTCATCTGGAGTTGGTGCATGGAACGCAGT
CGTTTTCGAGATCGTGATGACATTCGGACTAGTGTACACAGTGTATGCCA
CAGCCCTGGACCCAAAGAAGGGAAGCTTGGGGACAATTGCACCAATTGCA
ATTGGTTTCATTGTAGGAGCCAACATCTTGGCGGGCGGGGCGTTTGATGG
AGCTTCAATGAACCCAGCAGTGTCATTCGGGCCAGCTTTGGTAAGCTGGA
GCTGGGACAACCACTGGGTCTACTGGGTCGGGCCACTCATCGGTGGTGGG
CTAGCTGGCATCGTCTACGAGTTCTTCTTCATTAACCAGTCTCACGAGCA
GCTCCCAAACACCGAGTACTAAGCTAAAAGCTTGCCATCTATGTGAGTTT
GCACGGATGTTGTATTTATTTTGCTCTTACCGAGGTTCTTTCTTTTTATC
TTTATTTCGCGTTAATTTAAAAAAACAAAAAAAAAAAA
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