EY659590 | Citrus Genome Database

Overview


Name	EY659590
Unique Name	EY659590
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	925

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5270	contig	Csv1_Contig5270:177..1102.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: STK38_HUMAN (Serine/threonine-protein kinase 38 OS=Homo sapiens GN=STK38 PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.159e-29
Identity = 56/125 (44.80%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            SN TK++V   K  +EN Y   +   EER+ R+  LEK + +  + +EE+        +KETE++RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV H++AE
Sbjct:   13 SNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAE 137

HSP 2 Score: 38.891 bits (89), Expect = 1.159e-29
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  167 EFLPGGDMMTLLMKKDTLTEEE 188

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: ORB6_SCHPO (Serine/threonine-protein kinase orb6 OS=Schizosaccharomyces pombe GN=orb6 PE=1 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.323e-29
Identity = 55/122 (45.08%), Postives = 82/122 (67.21%), Query Frame = 2

Query:  356 TKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            T  KV   K+YIE++YK  +    ER +RR  LE++LA    SEE +N  L+   +KE++++R +R ++  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L K+EM +R Q+ HVKAE
Sbjct:   20 TLDKVQKTKKYIEHYYKVAVDHAVERNQRRINLEQRLATERGSEERKNRQLRASGEKESQFLRFRRTRLSLEDFSTIKVIGKGAFGEVRLVQKLDTGKIYAMKSLLKTEMFKRDQLAHVKAE 141

HSP 2 Score: 31.187 bits (69), Expect = 1.323e-29
Identity = 12/30 (40.00%), Postives = 18/30 (60.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+  DT +E      +  C
Sbjct:  171 EFLPGGDLMTMLINYDTFSEDVTRFYMAEC 200

HSP 3 Score: 30.8018 bits (68), Expect = 1.323e-29
Identity = 15/29 (51.72%), Postives = 18/29 (62.07%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R+LL E D   +  LY +F D  YLYLIM
Sbjct:  142 RDLLVESDSPWVVSLYYAFQDSLYLYLIM 170

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_PNECA (Serine/threonine-protein kinase CBK1 OS=Pneumocystis carinii GN=CBK1 PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.714e-29
Identity = 54/125 (43.20%), Postives = 81/125 (64.80%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            +  T ++ A+ K  +E+ YK  ++   ER +RR   E KLA    SEE +   L  L +KE++++RL+R K+  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM ++ Q+ HVKAE
Sbjct:   50 TKTTHERAASVKLKLEHLYKVTVEQTVERNQRRMDFEAKLAQDRGSEERKKRQLNSLGQKESQFLRLRRTKLSLNDFHTVKVIGKGAFGEVRLVQKIDTGKIYAMKTLLKSEMFKKDQLAHVKAE 174

HSP 2 Score: 34.2686 bits (77), Expect = 1.714e-29
Identity = 16/29 (55.17%), Postives = 20/29 (68.97%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LAE D   +  LY SF D +YLYLIM
Sbjct:  175 RDVLAESDSPWVVSLYYSFQDSQYLYLIM 203

HSP 3 Score: 31.9574 bits (71), Expect = 1.714e-29
Identity = 12/30 (40.00%), Postives = 19/30 (63.33%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L++ DT +E      +  C
Sbjct:  204 EFLPGGDLMTMLIKYDTFSEDVTRFYIAEC 233

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: ST38L_MOUSE (Serine/threonine-protein kinase 38-like OS=Mus musculus GN=Stk38l PE=1 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.330e-29
Identity = 54/125 (43.20%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            SN T+++V  AK  +EN Y   +   EER+ R+  LE  + +  +++EE+        +KETE++RL+R ++G DDFE L +I +GAFGEVR+ ++  TGH+YAMK L+K++ML + QV H++AE
Sbjct:   14 SNHTRERVTVAKLTLENFYSNLILQHEERETRQKKLEVAMEEEGLADEEKKLRRSQHARKETEFLRLKRTRLGLDDFESLKVIARGAFGEVRLVQKKDTGHIYAMKILRKADMLEKEQVAHIRAE 138

HSP 2 Score: 38.891 bits (89), Expect = 3.330e-29
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  168 EFLPGGDMMTLLMKKDTLTEEE 189

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: TRC_DROME (Serine/threonine-protein kinase tricorner OS=Drosophila melanogaster GN=trc PE=1 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.330e-29
Identity = 58/125 (46.40%), Postives = 83/125 (66.40%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S+ T  K   AK  +EN+Y   +    ERK+R   LE +L D  +SE ++        +KETEY+RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV HV+AE
Sbjct:   17 SDHTLDKATKAKVTLENYYSNLVTQYGERKQRLAKLEAQLKDESLSEAQRQEKRLQHAQKETEYLRLKRLRLGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLRKADMLEKEQVAHVRAE 141

HSP 2 Score: 36.965 bits (84), Expect = 3.330e-29
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTE 866
            E+LPGG MMTLLM++DTL+E
Sbjct:  171 EFLPGGDMMTLLMKKDTLSE 190

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: STK38_BOVIN (Serine/threonine-protein kinase 38 OS=Bos taurus GN=STK38 PE=1 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 4.336e-29
Identity = 55/125 (44.00%), Postives = 85/125 (68.00%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S+ TK++V   K  +EN Y   +   EER+ R+  LEK + +  + +EE+        +KETE++RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV H++AE
Sbjct:   13 SSHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAE 137

HSP 2 Score: 38.891 bits (89), Expect = 4.336e-29
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERK 872
            E+LPGG MMTLLM++DTLTE +
Sbjct:  167 EFLPGGDMMTLLMKKDTLTEEE 188

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: TRC_DROPS (Serine/threonine-protein kinase tricorner OS=Drosophila pseudoobscura pseudoobscura GN=trc PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 5.646e-29
Identity = 57/125 (45.60%), Postives = 83/125 (66.40%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S+ T  K   AK  +EN+Y   +    ERK+R   LE +L D  ++E ++        +KETEY+RL+R ++G +DFE L +IG+GAFGEVR+ ++  TGHVYAMK L+K++ML + QV HV+AE
Sbjct:   16 SDHTLDKATKAKVTLENYYSNLVTQYGERKQRLAKLEAQLKDESLTESQRQEKRLQHAQKETEYLRLKRLRLGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLRKADMLEKEQVAHVRAE 140

HSP 2 Score: 36.965 bits (84), Expect = 5.646e-29
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTE 866
            E+LPGG MMTLLM++DTL+E
Sbjct:  170 EFLPGGDMMTLLMKKDTLSE 189

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_DEBHA (Serine/threonine-protein kinase CBK1 OS=Debaryomyces hansenii GN=CBK1 PE=3 SV=2)

HSP 1 Score: 108.227 bits (269), Expect = 1.370e-28
Identity = 56/127 (44.09%), Postives = 85/127 (66.93%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEV--SEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            + + + K ++ K  +EN+Y+  +    ER +RR  LE KL + E   SEE +N  L+ L KKE++++RL+R K+  +DF  + +IGKGAFGEVR+ ++  TG +YAMK L KSEM ++ Q+ HVKAE
Sbjct:  237 NKIAQDKASSIKLKLENYYQMSVAHAIERNQRRLDLEHKLLNEESGSSEERKNRQLQNLGKKESQFLRLRRTKLSLEDFNTVKVIGKGAFGEVRLVQKRDTGKIYAMKTLLKSEMYKKDQLAHVKAE 363

HSP 2 Score: 30.8018 bits (68), Expect = 1.370e-28
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA  D   +  LY SF D +YLYLIM
Sbjct:  364 RDVLAGSDSPWVVSLYYSFQDAQYLYLIM 392

HSP 3 Score: 29.261 bits (64), Expect = 1.370e-28
Identity = 12/30 (40.00%), Postives = 17/30 (56.67%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L+R    TE      +  C
Sbjct:  393 EFLPGGDLMTMLIRWQIFTEDITRFYMAEC 422

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: NDRB_DICDI (Probable serine/threonine-protein kinase ndrB OS=Dictyostelium discoideum GN=ndrB PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.653e-25
Identity = 54/132 (40.91%), Postives = 84/132 (63.64%), Query Frame = 2

Query:  335 EEVPSNVTKQKVAAA---KQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            EE   N +K+ + AA   K  IE +Y    KSL+ER +RR  LEKK+ +  + EE+++   + L+KKETEY++ +R ++    FE + +IG+GAFGEVR+ +       +AMKKL KS+M+ + Q  HV++E
Sbjct:   47 EEEDINFSKETLEAAMATKVSIEQYYTSLFKSLKERDDRRCFLEKKMEELNLREEQKSVKRRELDKKETEYIKSRRIRLTGHSFESIRIIGRGAFGEVRLVKMKKNNKFFAMKKLDKSKMIEKHQTIHVRSE 178

HSP 2 Score: 31.9574 bits (71), Expect = 2.653e-25
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTE 866
            EY+PGG MMT L++ DT TE
Sbjct:  213 EYVPGGDMMTQLIKYDTFTE 232

HSP 3 Score: 23.8682 bits (50), Expect = 2.653e-25
Identity = 11/18 (61.11%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  754 IGKLYCSFPDDEYLYLIM 807
            I  LY SF D  +LYLIM
Sbjct:  195 IVSLYYSFQDANFLYLIM 212

[Close]

BLAST of EY659590 vs. ExPASy Swiss-Prot
Match: CBK1_YARLI (Serine/threonine-protein kinase CBK1 OS=Yarrowia lipolytica GN=CBK1 PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 9.730e-25
Identity = 50/125 (40.00%), Postives = 73/125 (58.40%), Query Frame = 2

Query:  347 SNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCRENATGHVYAMKKLKKSEMLRRGQVEHVKAE 721
            S   + +     Q I  +YK  ++   ER +RR+  E  +  A  SEE +N +L    KKET Y+R++R +M  +DF  + +IGKGAFGEVR+ ++   G +YAMK + K EM  + Q  HVKAE
Sbjct:  132 SKTARDRAHNTTQVISTYYKGIVQHAIERHQRRSAAEASVEQA-TSEERRNRVLTNYGKKETAYLRMRRTRMALEDFVTVKVIGKGAFGEVRLVQKRDNGKIYAMKTMLKKEMDMKEQWAHVKAE 255

HSP 2 Score: 33.8834 bits (76), Expect = 9.730e-25
Identity = 17/29 (58.62%), Postives = 20/29 (68.97%), Query Frame = 1

Query:  721 RNLLAEXDXHCIGKLYCSFPDDEYLYLIM 807
            R++LA+ D   I  LY SF DD YLYLIM
Sbjct:  256 RDVLADSDSPWIVSLYFSFQDDLYLYLIM 284

HSP 3 Score: 29.6462 bits (65), Expect = 9.730e-25
Identity = 11/30 (36.67%), Postives = 18/30 (60.00%), Query Frame = 3

Query:  807 EYLPGGSMMTLLMRRDTLTERKPXLLLGNC 896
            E+LPGG +MT+L++ D  +E      +  C
Sbjct:  285 EFLPGGDLMTMLIKYDVFSEDITRFYIAEC 314

The following BLAST results are available for this feature:

BLAST of EY659590 vs. ExPASy Swiss-Prot

BLAST of EY659590 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34


Match Name	E-value	Identity	Description
STK38_HUMAN	1.159e-29	44.80	Serine/threonine-protein kinase 38 OS=Homo sapiens...	[more]
ORB6_SCHPO	1.323e-29	45.08	Serine/threonine-protein kinase orb6 OS=Schizosacc...	[more]
CBK1_PNECA	1.714e-29	43.20	Serine/threonine-protein kinase CBK1 OS=Pneumocyst...	[more]
ST38L_MOUSE	3.330e-29	43.20	Serine/threonine-protein kinase 38-like OS=Mus mus...	[more]
TRC_DROME	3.330e-29	46.40	Serine/threonine-protein kinase tricorner OS=Droso...	[more]
STK38_BOVIN	4.336e-29	44.00	Serine/threonine-protein kinase 38 OS=Bos taurus G...	[more]
TRC_DROPS	5.646e-29	45.60	Serine/threonine-protein kinase tricorner OS=Droso...	[more]
CBK1_DEBHA	1.370e-28	44.09	Serine/threonine-protein kinase CBK1 OS=Debaryomyc...	[more]
NDRB_DICDI	2.653e-25	40.91	Probable serine/threonine-protein kinase ndrB OS=D...	[more]
CBK1_YARLI	9.730e-25	40.00	Serine/threonine-protein kinase CBK1 OS=Yarrowia l...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-011-H04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659590 ID=EY659590; Name=EY659590; organism=Citrus sinensis; type=EST; length=925bp
CTTTCATTTGTTGTGACCGAAAATCGCGGGTTTCTTTAATTTGTCTCAGG
ATAGCAGATCTTTGATACTCTGGGAATCCTTGATTCGAATGAACCTAAAT
GTTTCCGTTTGTGAAATGAGATTCGTGATTGATGAGAGTATATTTTTCAG
TAATTTCTCAAATAATTTGTACATTTAGGAGTGTGACCGTCGGGGAAAGT
CATATATAGATTTGAAAAAAATGGACTCTGCAAGATGTTGGTTTAACAAG
CTGAGGTCAAAAGACAAGGTAAAGCCTTCCAAGAAAAAGGAAACTGCAGG
CCATGGAAAGGAGGTGTCAAAAGCTCAAACAAGTGAAGAAGTTCCATCAA
ATGTTACCAAGCAGAAGGTTGCAGCTGCAAAACAGTATATTGAAAACCAT
TACAAGAAGCAGATGAAAAGCCTCGAGGAGAGGAAGGAGCGACGCAATAT
TTTGGAAAAGAAGTTAGCTGATGCAGAGGTTTCTGAGGAAGAGCAAAATA
ATTTACTAAAGTATTTAGAGAAGAAGGAAACGGAATACATGCGCCTTCAG
AGGCATAAAATGGGTGCTGATGATTTTGAGCCATTGACCATGATAGGGAA
GGGTGCATTTGGAGAGGTTAGAGTATGTAGGGAGAATGCAACCGGCCATG
TATATGCTATGAAAAAGCTTAAGAAATCAGAGATGCTTCGTAGAGGACAG
GTTGAACATGTGAAAGCTGAAGAAATCTACTTGCTGAAGNTGATAGNCAT
TGTATTGGTAAGCTATATTGTTCCTTTCCAGATGACGAGTATCTATATCT
GATTATGAATATCTACCTGGTGGNAGTATGATGACTTTACTGATGCGTAG
GGACACATTGACTGAAAGGAAGCCAGNTTTATTGTTGGGAAACTGTNCTA
GCTATTGATCTTNCCTAAACATAAT