EY659533

Overview
NameEY659533
Unique NameEY659533
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length895
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK1_MOUSE (Rho-associated protein kinase 1 OS=Mus musculus GN=Rock1 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.469e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + +    +++  +M A+D+E + +IG+GAFGEV++ R K+T  VYAMK L K EM++R
Sbjct:   48 KNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKR 115

HSP 2 Score: 31.9574 bits (71), Expect = 2.469e-11
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD YLY++MEY+PGG
Sbjct:  145 DDRYLYMVMEYMPGG 159
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK1_HUMAN (Rho-associated protein kinase 1 OS=Homo sapiens GN=ROCK1 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.469e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + +    +++  +M A+D+E + +IG+GAFGEV++ R K+T  VYAMK L K EM++R
Sbjct:   48 KNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKR 115

HSP 2 Score: 31.9574 bits (71), Expect = 2.469e-11
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD YLY++MEY+PGG
Sbjct:  145 DDRYLYMVMEYMPGG 159
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: CBK1_EMENI (Serine/threonine-protein kinase cbk1 OS=Emericella nidulans GN=cbk1 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.496e-11
Identity = 48/169 (28.40%), Postives = 78/169 (46.15%), Query Frame = 3 
Query:  330 PSNVTKQKVAAAKQYIENHYKKQMKSLEERKERRNILEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQR-HKMGADD------------FEPLTMIGKGAFGEVRVCREKATGH-------------------------VYAMKKLKKSEMLRRGQVEHVKAEXIYLL 722
            P+  T +  A  K ++E H+   +  L+ R +RR  L++ +    +S EE   + K+   +E +Y+R  R  K    D            FEPL ++G+G+FG VR+ REK T                           V+AMK ++KS M+R  Q  H++AE  +L+
Sbjct:  270 PTIQTTEATAIVKVFLETHFHTLLSGLDARTQRRLELDQYIETFPLSPEEVVRVRKHWVTQERDYLRQYRVLKSRPQDKTSRAGTASLAGFEPLKILGRGSFGVVRLVREKRTDEQTQSGRVPLAPKTNHRQAMTGVKKDVFAMKVIRKSVMIRNCQEAHLRAERDFLV 438

HSP 2 Score: 24.6386 bits (52), Expect = 2.496e-11
Identity = 9/20 (45.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  751 ILXLPDDEYLYLMMEYLPGG 810
            I    D ++LYL+M+Y+ GG
Sbjct:  450 IASFQDQKHLYLVMDYMVGG 469
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK2_BOVIN (Rho-associated protein kinase 2 OS=Bos taurus GN=ROCK2 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.202e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + E    +++  +M A+D++ + +IG+GAFGEV++ R KA+  VYAMK L K EM++R
Sbjct:   64 KNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKR 131

HSP 2 Score: 32.3426 bits (72), Expect = 3.202e-11
Identity = 11/15 (73.33%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD+YLY++MEY+PGG
Sbjct:  161 DDKYLYMVMEYMPGG 175
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: DMPK_MOUSE (Myotonin-protein kinase OS=Mus musculus GN=Dmpk PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.271e-11
Identity = 34/76 (44.74%), Postives = 48/76 (63.16%), Query Frame = 3 
Query:  498 YLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRRGQVEHVKAEXIYLLK 725
            +L+  E    RL+  ++  DDFE L +IG+GAF EV V + K TG VYAMK + K +ML+RG+V   + E   L+K
Sbjct:   50 FLQWVEPIAARLKEVRLQRDDFEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDMLKRGEVSCFREERDVLVK 125

HSP 2 Score: 26.5646 bits (57), Expect = 3.271e-11
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            D+ YLYL+MEY  GG
Sbjct:  140 DENYLYLVMEYYVGG 154
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: MRCKG_MOUSE (Serine/threonine-protein kinase MRCK gamma OS=Mus musculus GN=Cdc42bpg PE=2 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 4.143e-11
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 3 
Query:  438 LEKKLADAEVSEEEQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRRGQVEHVKAEXIYLLK 725
            + ++L+ A +  E   N+ ++L        +++  ++  DDFE L +IG+GAFGEV V R++ +G ++AMK L K EML+R +    + E   L+K
Sbjct:   32 VHQELSSAPLRRER--NVAQFLSWASPFVTKVKELRLQRDDFEILKVIGRGAFGEVAVVRQRGSGQIFAMKMLHKWEMLKRAETACFREERDVLVK 125

HSP 2 Score: 27.7202 bits (60), Expect = 4.143e-11
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            D+EYLYL+M+Y  GG
Sbjct:  140 DEEYLYLVMDYYAGG 154
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK2_MOUSE (Rho-associated protein kinase 2 OS=Mus musculus GN=Rock2 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.155e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + E    +++  +M A+D++ + +IG+GAFGEV++ R KA+  VYAMK L K EM++R
Sbjct:   64 KNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKR 131

HSP 2 Score: 31.9574 bits (71), Expect = 4.155e-11
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD YLY++MEY+PGG
Sbjct:  161 DDRYLYMVMEYMPGG 175
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK2_HUMAN (Rho-associated protein kinase 2 OS=Homo sapiens GN=ROCK2 PE=1 SV=4)

HSP 1 Score: 56.9954 bits (136), Expect = 4.155e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + E    +++  +M A+D++ + +IG+GAFGEV++ R KA+  VYAMK L K EM++R
Sbjct:   64 KNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKR 131

HSP 2 Score: 31.9574 bits (71), Expect = 4.155e-11
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD YLY++MEY+PGG
Sbjct:  161 DDRYLYMVMEYMPGG 175
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: ROCK2_RAT (Rho-associated protein kinase 2 OS=Rattus norvegicus GN=Rock2 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.156e-11
Identity = 27/68 (39.71%), Postives = 46/68 (67.65%), Query Frame = 3 
Query:  477 EQNNLLKYLEKKETEYMRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRR 680
            +  N+  +L + E    +++  +M A+D++ + +IG+GAFGEV++ R KA+  VYAMK L K EM++R
Sbjct:   55 KNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKR 122

HSP 2 Score: 31.9574 bits (71), Expect = 4.156e-11
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            DD YLY++MEY+PGG
Sbjct:  152 DDRYLYMVMEYMPGG 166
[Close]
BLAST of EY659533 vs. ExPASy Swiss-Prot
Match: MRCKG_HUMAN (Serine/threonine-protein kinase MRCK gamma OS=Homo sapiens GN=CDC42BPG PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.977e-11
Identity = 33/67 (49.25%), Postives = 44/67 (65.67%), Query Frame = 3 
Query:  525 MRLQRHKMGADDFEPLTMIGKGAFGEVRVCREKATGHVYAMKKLKKSEMLRRGQVEHVKAEXIYLLK 725
            +RLQR     DDFE L +IG+GAFGEV V R++ TG ++AMK L K EML+R +    + E   L+K
Sbjct:   64 LRLQR-----DDFEILKVIGRGAFGEVTVVRQRDTGQIFAMKMLHKWEMLKRAETACFREERDVLVK 125

HSP 2 Score: 27.7202 bits (60), Expect = 6.977e-11
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 1 
Query:  766 DDEYLYLMMEYLPGG 810
            D+EYLYL+M+Y  GG
Sbjct:  140 DEEYLYLVMDYYAGG 154
The following BLAST results are available for this feature:
BLAST of EY659533 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
ROCK1_MOUSE2.469e-1139.71Rho-associated protein kinase 1 OS=Mus musculus GN... [more]
ROCK1_HUMAN2.469e-1139.71Rho-associated protein kinase 1 OS=Homo sapiens GN... [more]
CBK1_EMENI2.496e-1128.40Serine/threonine-protein kinase cbk1 OS=Emericella... [more]
ROCK2_BOVIN3.202e-1139.71Rho-associated protein kinase 2 OS=Bos taurus GN=R... [more]
DMPK_MOUSE3.271e-1144.74Myotonin-protein kinase OS=Mus musculus GN=Dmpk PE... [more]
MRCKG_MOUSE4.143e-1134.38Serine/threonine-protein kinase MRCK gamma OS=Mus ... [more]
ROCK2_MOUSE4.155e-1139.71Rho-associated protein kinase 2 OS=Mus musculus GN... [more]
ROCK2_HUMAN4.155e-1139.71Rho-associated protein kinase 2 OS=Homo sapiens GN... [more]
ROCK2_RAT4.156e-1139.71Rho-associated protein kinase 2 OS=Rattus norvegic... [more]
MRCKG_HUMAN6.977e-1149.25Serine/threonine-protein kinase MRCK gamma OS=Homo... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-011-B11-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659533 ID=EY659533; Name=EY659533; organism=Citrus sinensis; type=EST; length=895bp
CGCTCATTGAGGATTTTAATTTTGGTTATTTTTCGGATAGCAGATCTTTG
ATACTCTGGGAATCCTTGATTCGAATGAACCTAAATGTTTCCGTTTGTGA
AATGAGATTCTTGATTGATGAGAGTATATTTTTCAGTAATTTCTCAAATA
ATTTGTACATTTATGAGTGTGACCGTCGGGGAAAGTCATATATAGATTTG
AAAAAAATGGACTCTGCAAGATGTTGGTTTAACAAGCTGAGGTCAAAAGA
CAAGGTGAAGCCTTCCAAGAAAAAGGAAACTGCAGGCCATGGAAAGGAGG
TGTCAAAAGCTCAAACAAGCGAAGAAGTTCCATCAAATGTTACCAAGCAG
AAGGTTGCAGCTGCAAAACAGTATATTGAAAACCATTACAAGAAGCAGAT
GAAAAGCCTCGAGGAGAGGAAGGAGCGACGCAATATTTTGGAAAAGAAGT
TAGCTGATGCAGAGGTTTCTGAGGAAGAGCAAAATAATTTACTAAAGTAT
TTAGAGAAGAAGGAAACGGAATACATGCGCCTTCAGAGGCATAAAATGGG
TGCTGATGATTTTGAGCCATTGACCATGATAGGGAAGGGTGCATTTGGAG
AGGTTAGAGTATGTAGGGAGAAGGCAACCGGCCATGTATATGCTATGAAA
AAGCTTAAGAAATCAGAGATGCTTCGTAGAGGACAGGTTGAACATGTGAA
AGCTGAAAGNATCTACTTGCTGAAGTTGANTAGCATTGTNATTGTAAGCT
ATATTGTNCCTTCCAGATGACGAGTATCTATATCTGATGATGGAATATCT
ACCTGGTGGAGATATGATGACTTTACTGATGCGTTAGGACACATTGACTN
GAGATGAAGCAAGTTTTATGTTGGGGGAACTGTCCTANCTATTGA
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