EY659431 | Citrus Genome Database

Overview


Name	EY659431
Unique Name	EY659431
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	808

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13130	contig	Csv1_Contig13130:769..1578.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CFAD_DICDI (Counting factor associated protein D OS=Dictyostelium discoideum GN=cfaD PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.101e-27
Identity = 53/126 (42.06%), Postives = 80/126 (63.49%), Query Frame = 3

Query:   93 KNGVCKFSSENV-GVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMG-KNMCGIATCASYPV 461
            +NG+C+  +    GV +   VN+T G+E  LQ+A+    PV++A +  VD FR+Y SGVY++  C N   D++H V+A+GYG   G  Y+L+KNSW  NWG  GY  M     N+CG+++ A+YP+
Sbjct:  402 QNGLCRDRTVTPSGVSITGYVNVTSGSESALQNAIATTGPVAIAIDASVDDFRYYMSGVYNNPACKNGLDDLDHEVLAIGYGTYQGQDYFLVKNSWSTNWGMDGYVYMARNDNNLCGVSSQATYPI 527

HSP 2 Score: 35.039 bits (79), Expect = 1.101e-27
Identity = 15/29 (51.72%), Postives = 17/29 (58.62%), Query Frame = 1

Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPY 87
            Q C GG  S AF+Y+   G L TE  YPY
Sbjct:  371 QGCGGGFASSAFQYVMEIGSLATESNYPY 399

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATJ_MOUSE (Cathepsin J OS=Mus musculus GN=Ctsj PE=2 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 1.881e-27
Identity = 60/128 (46.88%), Postives = 75/128 (58.59%), Query Frame = 3

Query:   93 KNGVCKFSSENVGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVV-DGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYG----VEDGVPYWLIKNSWGENWGDHGYFKMEMG-KNMCGIATCASYP 458
            K+G C++ SEN    + D VN+    E  L  AV  + PVS A +   D FRFY  G+Y    C +    VNHAV+ VGYG    V+DG  YWLIKNSWGE WG +GY ++     N CGIA+ ASYP
Sbjct:  207 KDGPCRYRSENASANITDYVNLPPN-ELYLWVAVASIGPVSAAIDASHDSFRFYNGGIYYEPNCSS--YFVNHAVLVVGYGSEGDVKDGNNYWLIKNSWGEEWGMNGYMQIAKDHNNHCGIASLASYP 331

HSP 2 Score: 38.891 bits (89), Expect = 1.881e-27
Identity = 16/30 (53.33%), Postives = 18/30 (60.00%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C  G   QAFEY+  N GL+ E  YPY GK
Sbjct:  178 CQSGTAHQAFEYVLKNKGLEAEATYPYEGK 207

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_PIG (Cathepsin K OS=Sus scrofa GN=CTSK PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.882e-27
Identity = 51/100 (51.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V PVSVA +  +  F+FY  GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  231 GNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDENCNSD--NLNHAVLAVGYGIQKGKKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 328

HSP 2 Score: 42.3578 bits (98), Expect = 1.882e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  178 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 207

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_BOVIN (Cathepsin K OS=Bos taurus GN=CTSK PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 1.882e-27
Identity = 50/100 (50.00%), Postives = 66/100 (66.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V P+SVA +  +  F+FY+ GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  230 GNEKALKRAVARVGPISVAIDASLTSFQFYRKGVYYDENCNSD--NLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 327

HSP 2 Score: 42.3578 bits (98), Expect = 1.882e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  177 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 206

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CYSP3_DICDI (Cysteine proteinase 3 OS=Dictyostelium discoideum GN=cprC PE=3 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 2.449e-27
Identity = 52/124 (41.94%), Postives = 77/124 (62.10%), Query Frame = 3

Query:   96 NGVCKFSSENVGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVV-DGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYPV 461
            N  CKF   +V  ++     I  G E++LQ+A+ L+ PVSVA +   + F+ Y +GVY    C +   D++H V+AVG G ++G  Y+++KNSWG +WG +GY  M   K N CGI+T ASYP+
Sbjct:  216 NDECKFQEGSVAAKITSYKEIEAGDENDLQNAL-LLNPVSVAIDASHNSFQLYTAGVYYEPACSSE--DLDHGVLAVGMGTDNGEDYYIVKNSWGPSWGLNGYIHMARNKDNNCGISTMASYPI 336

HSP 2 Score: 43.5134 bits (101), Expect = 2.449e-27
Identity = 19/31 (61.29%), Postives = 23/31 (74.19%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGKM 99
            CNGGL + AFEYI  N GL++EE YPY  K+
Sbjct:  185 CNGGLMTNAFEYIIKNNGLNSEEQYPYEMKV 215

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_MACMU (Cathepsin K OS=Macaca mulatta GN=CTSK PE=1 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.450e-27
Identity = 51/100 (51.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V PVSVA +  +  F+FY  GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  230 GNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSD--NLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 327

HSP 2 Score: 42.3578 bits (98), Expect = 2.450e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  177 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 206

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_MACFA (Cathepsin K OS=Macaca fascicularis GN=CTSK PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.450e-27
Identity = 51/100 (51.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V PVSVA +  +  F+FY  GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  230 GNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSD--NLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 327

HSP 2 Score: 42.3578 bits (98), Expect = 2.450e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  177 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 206

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_HUMAN (Cathepsin K OS=Homo sapiens GN=CTSK PE=1 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.450e-27
Identity = 51/100 (51.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V PVSVA +  +  F+FY  GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  230 GNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSD--NLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 327

HSP 2 Score: 42.3578 bits (98), Expect = 2.450e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  177 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 206

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATL1_SHEEP (Cathepsin L1 OS=Ovis aries GN=CTSL PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.472e-27
Identity = 48/101 (47.52%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  171 EDELQHAVGLVRPVSVAFEVV-DGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVED-GVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYPVV 464
            E  L  AV  V P+SVA +     F+FYKSG+Y    C +   D++H V+ VGYG E     +W++KNSWG  WG+ GY KM   + N CGIAT ASYP V
Sbjct:  119 EKALMKAVATVGPISVAIDAGHSSFQFYKSGIYYDPDCSSK--DLDHGVLVVGYGFEGTNNKFWIVKNSWGPEWGNKGYVKMAKDQNNHCGIATAASYPTV 217

HSP 2 Score: 52.7582 bits (125), Expect = 2.472e-27
Identity = 25/46 (54.35%), Postives = 30/46 (65.22%), Query Frame = 1

Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPYTGKMVSANSHLKMLASK 138
            Q CNGGL   AF+YIK NGGLD+EE+YPY     S N   +  A+K
Sbjct:   63 QGCNGGLMDNAFQYIKENGGLDSEESYPYEATDTSCNYKPEYSAAK 108

[Close]

BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATK_CANFA (Cathepsin K OS=Canis familiaris GN=CTSK PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.189e-27
Identity = 50/100 (50.00%), Postives = 65/100 (65.00%), Query Frame = 3

Query:  165 GAEDELQHAVGLVRPVSVAFEV-VDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYP 458
            G E  L+ AV  V P+SVA +  +  F+FY  GVY    C +   ++NHAV+AVGYG++ G  +W+IKNSWGENWG+ GY  M   K N CGIA  AS+P
Sbjct:  231 GNEKALKRAVARVGPISVAIDASLTSFQFYSKGVYYDENCNSD--NLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFP 328

HSP 2 Score: 42.3578 bits (98), Expect = 3.189e-27
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1

Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG  + AF+Y++ N G+D+E+AYPY G+
Sbjct:  178 CGGGYMTNAFQYVQKNRGIDSEDAYPYVGQ 207

The following BLAST results are available for this feature:

BLAST of EY659431 vs. ExPASy Swiss-Prot

BLAST of EY659431 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 174


Match Name	E-value	Identity	Description
CFAD_DICDI	1.101e-27	42.06	Counting factor associated protein D OS=Dictyostel...	[more]
CATJ_MOUSE	1.881e-27	46.88	Cathepsin J OS=Mus musculus GN=Ctsj PE=2 SV=2	[more]
CATK_PIG	1.882e-27	51.00	Cathepsin K OS=Sus scrofa GN=CTSK PE=2 SV=1	[more]
CATK_BOVIN	1.882e-27	50.00	Cathepsin K OS=Bos taurus GN=CTSK PE=2 SV=2	[more]
CYSP3_DICDI	2.449e-27	41.94	Cysteine proteinase 3 OS=Dictyostelium discoideum ...	[more]
CATK_MACMU	2.450e-27	51.00	Cathepsin K OS=Macaca mulatta GN=CTSK PE=1 SV=1	[more]
CATK_MACFA	2.450e-27	51.00	Cathepsin K OS=Macaca fascicularis GN=CTSK PE=2 SV...	[more]
CATK_HUMAN	2.450e-27	51.00	Cathepsin K OS=Homo sapiens GN=CTSK PE=1 SV=1	[more]
CATL1_SHEEP	2.472e-27	47.52	Cathepsin L1 OS=Ovis aries GN=CTSL PE=1 SV=1	[more]
CATK_CANFA	3.189e-27	50.00	Cathepsin K OS=Canis familiaris GN=CTSK PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-009-A10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659431 ID=EY659431; Name=EY659431; organism=Citrus sinensis; type=EST; length=808bp
CAGGTATGCAATGGTGGGTTGCCATCCCAAGCCTTTGAATACATCAAGTA
CAATGGTGGCCTTGATACTGAGGAAGCATATCCCTACACCGGAAAAATGG
TGTCTGCAAATTCTCATCTGAAAATGTTGGCGTCCAAGTCCTGGACTCAG
TCAACATTACCTTGGGTGCTGAAGATGAATTACAGCATGCAGTTGGTCTT
GTTCGACCTGTGAGTGTGGCATTTGAAGTAGTAGATGGATTCCGATTTTA
CAAGAGTGGAGTTTACAGCAGCACTAAATGTGGAAATACTCCCATGGATG
TGAACCATGCCGTCGTTGCTGTTGGGTACGGAGTTGAAGATGGTGTTCCA
TATTGGCTGATTAAGAACTCTTGGGGAGAGAACTGGGGCGATCATGGCTA
CTTTAAGATGGAGATGGGGAAGAACATGTGTGGTATTGCAACTTGTGCAT
CATACCCAGTTGTGGCTTAATCTGCTCCTGAAGAAATAGTTGGATCTGGC
TATCAGCAAGTCATTTGCTCATAAAACTTATATTATTTCACTCAAGAATG
ATAGCAGAATGGTTGGCTTTATGTACGAAATAAATTCCGAGATTAATGTC
CATATAATCTACAATAGCAATGCATGGCTGCTTGATGTTCAAAATAAATT
CTGAGATCTATGTCCATGTAAACAGTCATTGTGACTAGGACACCAACGAT
GTTATATATATTTTGTCAATGCAAGGTANTTGTTATATGGAAGCTTTANG
CAAATTCAATGCATTGCTTAAAAATTTGGTTGTCTTCTGCCCTAAAAAAA
AAAAAAAA