EY659431

Overview
NameEY659431
Unique NameEY659431
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length808
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13130 contig Csv1_Contig13130:769..1578. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATF_HUMAN (Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 6.169e-25
Identity = 48/122 (39.34%), Postives = 71/122 (58.20%), Query Frame = 3 
Query:  105 CKFSSENVGVQVLDSVNITLGAEDELQHAVGLVR--PVSVAFEVVDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGKNMCGIATCASYPVV 464
            C FS+E   V + DSV ++   ++E + A  L +  P+SVA     G +FY+ G+    +   +P  ++HAV+ VGYG    VP+W IKNSWG +WG+ GY+ +  G   CG+ T AS  VV
Sbjct:  366 CNFSAEKAKVYINDSVELS---QNEQKLAAWLAKRGPISVAINAF-GMQFYRHGISRPLRPLCSPWLIDHAVLLVGYGNRSDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASSAVV 483

HSP 2 Score: 43.8986 bits (102), Expect = 6.169e-25
Identity = 20/37 (54.05%), Postives = 24/37 (64.86%), Query Frame = 1 
Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPYTGKMVSAN 111
            + C GGLPS A+  IK  GGL+TE+ Y Y G M S N
Sbjct:  331 KACMGGLPSNAYSAIKNLGGLETEDDYSYQGHMQSCN 367
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATL2_BOVIN (Cathepsin L2 OS=Bos taurus GN=CTSL2 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.084e-25
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:  171 EDELQHAVGLVRPVSVAFEVVD-GFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVE----DGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYPVV 464
            E  L  AV  V P+SVA +     F+FYKSG+Y    C +   D++H V+ VGYG E    +   +W++KNSWG  WG +GY KM   + N CGIAT ASYP V
Sbjct:  233 EKALMKAVATVGPISVAIDAGHTSFQFYKSGIYYDPDCSSK--DLDHGVLVVGYGFEGTDSNNNKFWIVKNSWGPEWGWNGYVKMAKDQNNHCGIATAASYPTV 334

HSP 2 Score: 46.2098 bits (108), Expect = 8.084e-25
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1 
Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPY 87
            Q CNGGL   AF+YIK NG LD+EE+YPY
Sbjct:  176 QGCNGGLMDNAFQYIKDNGCLDSEESYPY 204
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATS_BOVIN (Cathepsin S OS=Bos taurus GN=CTSS PE=1 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 1.782e-24
Identity = 50/125 (40.00%), Postives = 68/125 (54.40%), Query Frame = 3 
Query:   96 NGVCKFSSENVGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVV-DGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYPVV 464
            +G C++  +N        + +  G+E+ L+ AV    PVSV  +     F  YK+GVY    C     +VNH V+ VGYG  DG  YWL+KNSWG ++GD GY +M     N CGIA   SYP +
Sbjct:  210 DGKCQYDVKNRAATCSRYIELPFGSEEALKEAVANKGPVSVGIDASHSSFFLYKTGVYYDPSC---TQNVNHGVLVVGYGNLDGKDYWLVKNSWGLHFGDQGYIRMARNSGNHCGIANYPSYPEI 331

HSP 2 Score: 40.0466 bits (92), Expect = 1.782e-24
Identity = 15/27 (55.56%), Postives = 21/27 (77.78%), Query Frame = 1 
Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPY 87
            CNGG  ++AF+YI  N G+D+E +YPY
Sbjct:  180 CNGGFMTEAFQYIIDNNGIDSEASYPY 206
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 3.921e-24
Identity = 51/130 (39.23%), Postives = 71/130 (54.62%), Query Frame = 3 
Query:   93 KNGVCKFSSEN-VGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVVDG-FRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVE-DGVPYWLIKNSWGENWGDHGYFKMEMG----KNMCGIATCASYPV 461
            + G C  S  N + V +    N+    ED L  AV   +PVSVA +     F+FY  GV++    G+   D+NH V  VGYG   DG  YW+++NSWG  WG+HGY +M+      + +CGIA   SYP+
Sbjct:  220 QEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVAN-QPVSVAIDAGGSDFQFYSEGVFT----GDCSTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPI 344

HSP 2 Score: 42.743 bits (99), Expect = 3.921e-24
Identity = 18/29 (62.07%), Postives = 20/29 (68.97%), Query Frame = 1 
Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPY 87
            Q CNGGL   AFE+IK  GG+ TE  YPY
Sbjct:  189 QGCNGGLMESAFEFIKQKGGITTESNYPY 217
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CYSP2_HORVU (Cysteine proteinase EP-B 2 OS=Hordeum vulgare GN=EPB2 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.636e-24
Identity = 50/116 (43.10%), Postives = 69/116 (59.48%), Query Frame = 3 
Query:  153 NITLGAEDELQHAVGLVRPVSVAFEVVD-GFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGV-EDGVPYWLIKNSWGENWGDHGYFKMEMGK----NMCGIATCASYPVVA*SAPE 482
            ++   +E++L  AV   +PVSVA E     F FY  GV++  +CG    +++H V  VGYGV EDG  YW +KNSWG +WG+ GY ++E        +CGIA  ASYPV   S P+
Sbjct:  250 DVPANSEEDLARAVAN-QPVSVAVEASGKAFMFYSEGVFTG-ECGT---ELDHGVAVVGYGVAEDGKAYWTVKNSWGPSWGEQGYIRVEKDSGASGGLCGIAMEASYPVKTYSKPK 360

HSP 2 Score: 45.4394 bits (106), Expect = 6.636e-24
Identity = 21/35 (60.00%), Postives = 22/35 (62.86%), Query Frame = 1 
Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGKMVSAN 111
            C GGL   AFEYIK NGGL TE AYPY     + N
Sbjct:  197 CQGGLMDNAFEYIKNNGGLITEAAYPYRAARGTCN 231
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.640e-24
Identity = 50/130 (38.46%), Postives = 72/130 (55.38%), Query Frame = 3 
Query:   93 KNGVCKFSSEN-VGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVVDG-FRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVE-DGVPYWLIKNSWGENWGDHGYFKMEMG----KNMCGIATCASYPV 461
            + G C  S  N + V +    N+ +  E+ L  AV   +PVSVA +     F+FY  GV++    G+   D+NH V  VGYG   DG  YW+++NSWG  WG+ GY +M+      + +CGIA  ASYP+
Sbjct:  220 QEGTCDESKVNDLAVSIDGHENVPVNDENALLKAVAN-QPVSVAIDAGGSDFQFYSEGVFT----GDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPI 344

HSP 2 Score: 45.0542 bits (105), Expect = 6.640e-24
Identity = 19/32 (59.38%), Postives = 22/32 (68.75%), Query Frame = 1 
Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            Q CNGGL   AFE+IK  GG+ TE  YPYT +
Sbjct:  189 QGCNGGLMESAFEFIKQKGGITTESNYPYTAQ 220
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATR_MOUSE (Cathepsin R OS=Mus musculus GN=Ctsr PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 6.651e-24
Identity = 49/130 (37.69%), Postives = 73/130 (56.15%), Query Frame = 3 
Query:   93 KNGVCKFSSENVGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVV-DGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVE----DGVPYWLIKNSWGENWGDHGYFKMEMGK-NMCGIATCASYPVV 464
            K+G C+++ +N   ++   V++   +ED L  AV  + P++   +   + F+ YK G+Y    C +    V H V+ VGYG +    DG  YWLIKNSWG+ WG  GY K+   K N CGIA+ A YP +
Sbjct:  208 KDGPCRYNPKNSKAEITGFVSLPQ-SEDILMAAVATIGPITAGIDASHESFKNYKGGIYHEPNCSSDT--VTHGVLVVGYGFKGIETDGNHYWLIKNSWGKRWGIRGYMKLAKDKNNHCGIASYAHYPTI 334

HSP 2 Score: 40.4318 bits (93), Expect = 6.651e-24
Identity = 16/30 (53.33%), Postives = 21/30 (70.00%), Query Frame = 1 
Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTGK 96
            C GG    AF+Y+ +NGGL++E  YPY GK
Sbjct:  179 CLGGDTYNAFQYVLHNGGLESEATYPYEGK 208
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 8.600e-24
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 3 
Query:  105 CKFSSENVGVQVLDSVNITLGAEDELQHAVGLVRPVSVAFEVVDGFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKMEMGKNMCGIATCASYPVV 464
            C FS++   V + DSV ++   E+++   +    P+SVA     G +FY+ G+    +   +P  ++HAV+ VGYG    +PYW IKNSWG +WG+ GY+ +  G   CG+ T AS  VV
Sbjct:  344 CNFSAQMAKVYINDSVELSRN-ENKIAAWLAQKGPISVAINAF-GMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNRSNIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASSAVV 461

HSP 2 Score: 40.817 bits (94), Expect = 8.600e-24
Identity = 19/42 (45.24%), Postives = 26/42 (61.90%), Query Frame = 1 
Query:    1 QVCNGGLPSQAFEYIKYNGGLDTEEAYPYTGKMVSANSHLKM 126
            + C GGLPS A+  IK  GGL+TE+ Y Y G + + N   +M
Sbjct:  309 KACLGGLPSNAYAAIKNLGGLETEDDYGYQGHVQTCNFSAQM 350
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: RD21A_ARATH (Cysteine proteinase RD21a OS=Arabidopsis thaliana GN=RD21A PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.119e-23
Identity = 48/128 (37.50%), Postives = 74/128 (57.81%), Query Frame = 3 
Query:   96 NGVCKFSSENVGVQVLDSV-NITLGAEDELQHAVGLVRPVSVAFEVVD-GFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKM----EMGKNMCGIATCASYPV 461
            +G C    +N  V  +DS  ++   +E+ L+ AV   +P+S+A E     F+ Y SG++  + CG     ++H VVAVGYG E+G  YW+++NSWG++WG+ GY +M          CGIA   SYP+
Sbjct:  230 DGTCDQIRKNAKVVTIDSYEDVPTYSEESLKKAVAH-QPISIAIEAGGRAFQLYDSGIFDGS-CGT---QLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPI 352

HSP 2 Score: 44.2838 bits (103), Expect = 1.119e-23
Identity = 18/29 (62.07%), Postives = 22/29 (75.86%), Query Frame = 1 
Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPYTG 93
            CNGGL   AFE+I  NGG+DT++ YPY G
Sbjct:  200 CNGGLMDYAFEFIIKNGGIDTDKDYPYKG 228
[Close]
BLAST of EY659431 vs. ExPASy Swiss-Prot
Match: CYSPL_SOLLC (Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.465e-23
Identity = 51/129 (39.53%), Postives = 76/129 (58.91%), Query Frame = 3 
Query:   93 KNGVCKFSSENVGVQVLDSV-NITLGAEDELQHAVGLVRPVSVAFEVVD-GFRFYKSGVYSSTKCGNTPMDVNHAVVAVGYGVEDGVPYWLIKNSWGENWGDHGYFKME----MGKNMCGIATCASYPV 461
            +NGVC    +N  V  +DS  ++ +  E  LQ AV   +PVS+A E     F+ YKSG+++  KCG     V+H VV  GYG E+G+ YW+++NSWG N  ++GY +++        +CG+A   SYPV
Sbjct:  110 RNGVCDQYRKNAKVVKIDSYEDVPVNNEKALQKAVAH-QPVSIALEAGGRDFQHYKSGIFTG-KCGTA---VDHGVVIAGYGTENGMDYWIVRNSWGANCRENGYLRVQRNVSSSSGLCGLAIEPSYPV 233

HSP 2 Score: 42.743 bits (99), Expect = 1.465e-23
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 1 
Query:    7 CNGGLPSQAFEYIKYNGGLDTEEAYPY 87
            C+GGL   AFE++  NGG+DTEE YPY
Sbjct:   81 CDGGLMDYAFEFVIKNGGIDTEEDYPY 107
The following BLAST results are available for this feature:
BLAST of EY659431 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 174
Match NameE-valueIdentityDescription
CATF_HUMAN6.169e-2539.34Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1[more]
CATL2_BOVIN8.084e-2546.15Cathepsin L2 OS=Bos taurus GN=CTSL2 PE=2 SV=1[more]
CATS_BOVIN1.782e-2440.00Cathepsin S OS=Bos taurus GN=CTSS PE=1 SV=2[more]
CYSEP_PHAVU3.921e-2439.23Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CYSP2_HORVU6.636e-2443.10Cysteine proteinase EP-B 2 OS=Hordeum vulgare GN=E... [more]
CYSEP_VIGMU6.640e-2438.46Vignain OS=Vigna mungo PE=1 SV=1[more]
CATR_MOUSE6.651e-2437.69Cathepsin R OS=Mus musculus GN=Ctsr PE=2 SV=1[more]
CATF_MOUSE8.600e-2437.50Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1[more]
RD21A_ARATH1.119e-2337.50Cysteine proteinase RD21a OS=Arabidopsis thaliana ... [more]
CYSPL_SOLLC1.465e-2339.53Low-temperature-induced cysteine proteinase (Fragm... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-009-A10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659431 ID=EY659431; Name=EY659431; organism=Citrus sinensis; type=EST; length=808bp
CAGGTATGCAATGGTGGGTTGCCATCCCAAGCCTTTGAATACATCAAGTA
CAATGGTGGCCTTGATACTGAGGAAGCATATCCCTACACCGGAAAAATGG
TGTCTGCAAATTCTCATCTGAAAATGTTGGCGTCCAAGTCCTGGACTCAG
TCAACATTACCTTGGGTGCTGAAGATGAATTACAGCATGCAGTTGGTCTT
GTTCGACCTGTGAGTGTGGCATTTGAAGTAGTAGATGGATTCCGATTTTA
CAAGAGTGGAGTTTACAGCAGCACTAAATGTGGAAATACTCCCATGGATG
TGAACCATGCCGTCGTTGCTGTTGGGTACGGAGTTGAAGATGGTGTTCCA
TATTGGCTGATTAAGAACTCTTGGGGAGAGAACTGGGGCGATCATGGCTA
CTTTAAGATGGAGATGGGGAAGAACATGTGTGGTATTGCAACTTGTGCAT
CATACCCAGTTGTGGCTTAATCTGCTCCTGAAGAAATAGTTGGATCTGGC
TATCAGCAAGTCATTTGCTCATAAAACTTATATTATTTCACTCAAGAATG
ATAGCAGAATGGTTGGCTTTATGTACGAAATAAATTCCGAGATTAATGTC
CATATAATCTACAATAGCAATGCATGGCTGCTTGATGTTCAAAATAAATT
CTGAGATCTATGTCCATGTAAACAGTCATTGTGACTAGGACACCAACGAT
GTTATATATATTTTGTCAATGCAAGGTANTTGTTATATGGAAGCTTTANG
CAAATTCAATGCATTGCTTAAAAATTTGGTTGTCTTCTGCCCTAAAAAAA
AAAAAAAA
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