EY659290 | Citrus Genome Database

Overview


Name	EY659290
Unique Name	EY659290
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	906

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH1_PSEPU (Dihydrolipoyl dehydrogenase OS=Pseudomonas putida GN=lpdV PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.820e-19
Identity = 47/124 (37.90%), Postives = 72/124 (58.06%), Query Frame = 2

Query:  113 LLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVAEIWK 481
            LL   GVK+  G  K++   +VEV   DG ++    +H+L+ATGS +   P +P     I+S EAL+ + LP+  VV+GGGYI +E    +R +G+ V ++  +E  L  +D E+ A VAE  K
Sbjct:  106 LLKKHGVKVVHGWAKVLDGKQVEV---DGQRIQ--CEHLLLATGSSSVELPMLPLGGPVISSTEALAPKALPQHLVVVGGGYIGLELGIAYRKLGAQVSVVEARERILPTYDSELTAPVAESLK 224

HSP 2 Score: 42.743 bits (99), Expect = 4.820e-19
Identity = 26/90 (28.89%), Postives = 52/90 (57.78%), Query Frame = 1

Query:  472 NLEGRGINLHPRTTIKELIKSEESIKVITDHGE----EIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            +L+  GI LH    +   ++  E+  ++ + G+     + AD VL A GR P TK  NL+ + ++++   A+ +D+  +T++ ++WA+GD
Sbjct:  222 SLKKLGIALH----LGHSVEGYENGCLLANDGKGGQLRLEADRVLVAVGRRPRTKGFNLECLDLKMNGA-AIAIDERCQTSMHNVWAIGD 306

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH_SCHPO (Dihydrolipoyl dehydrogenase, mitochondrial OS=Schizosaccharomyces pombe GN=dld1 PE=2 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 8.132e-19
Identity = 43/142 (30.28%), Postives = 75/142 (52.82%), Query Frame = 2

Query:    2 DARSYGWEVHEINDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTK-LSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLL 412
            D +  G +V  ++  +  ++++ K D +  L    + L     V+  +G G  + P  + V  +DG    +  AK+ +IATGS  +  P PG    ++  ++S  ALSL E+PK+  VLGGG I +E  S+W  +G+ V ++
Sbjct:  108 DTKRRGIDVSGVS-VNLSQMMKAKDDSVKSLTSGIEYLFKKNKVEYAKGTGSFIDPQTLSVKGIDGAADQTIKAKNFIIATGSEVK--PFPGVTIDEKKIVSSTGALSLSEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVV 246

HSP 2 Score: 45.8246 bits (107), Expect = 8.132e-19
Identity = 28/94 (29.79%), Postives = 47/94 (50.00%), Query Frame = 1

Query:  469 RNLEGRGINLHPRTTIKELIKSEESIKVITDH-----GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGDGT 735
            R +  +GI     T +     + +S++V  ++      E    DV+L A GR P T+ L L  + + +D++  V +D   RTN+P I  +GD T
Sbjct:  266 RIISKQGIKFKTSTKLLSAKVNGDSVEVEIENMKNNKRETYQTDVLLVAIGRVPYTEGLGLDKLGISMDKSNRVIMDSEYRTNIPHIRVIGDAT 359

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_PSEPW (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain W619) GN=sthA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 8.148e-19
Identity = 46/126 (36.51%), Postives = 63/126 (50.00%), Query Frame = 2

Query:  101 IYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            I K++ S  G      V L+ G G       VEV   +G     +AKHI+IATGSR  R A I      +  SD  LSL   P++ +V G G I  E+ASI+ G+G  V+L+  +   L   D E+
Sbjct:   93 ISKQVASRTGYYARNRVDLFFGTGSFADEQTVEVVCPNGVVEKLNAKHIIIATGSRPYRPADIDFHHPRVYDSDTILSLSHTPRKLIVYGAGVIGCEYASIFSGLGVLVELVDNRGQLLSFLDSEI 218

HSP 2 Score: 52.373 bits (124), Expect = 8.148e-19
Identity = 23/55 (41.82%), Postives = 38/55 (69.09%), Query Frame = 1

Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I AD +L+  GR  NT +L L+ + ++V+  G +EVD+  RT+VP+I+  GD
Sbjct:  256 GKKIKADALLWCNGRTGNTDKLGLENIGIKVNSRGQIEVDEAYRTSVPNIYGAGD 310

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH_RHOCA (Dihydrolipoyl dehydrogenase OS=Rhodobacter capsulatus GN=lpd PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 8.152e-19
Identity = 54/170 (31.76%), Postives = 90/170 (52.94%), Query Frame = 2

Query:   23 EVHEIND-FDWKKLLQKKTD-EILRLNGIYKR---LLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQEL----AITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVAEIWKGGELICTR 505
            E+HE+++ F+   L+  K   +  +++G  K    L     V    G G I  P +V+V    G ++ ++AK+I+IATGS +  + +PG E+     +TS  ALSL ++PK  VV+G G I +E  S++  +G+ V ++   +    G D       AE+ KG + I TR
Sbjct:   59 ELHEVHENFEKMGLMGAKVKVDWAKIDGNTKGIEFLFKKNKVTYLRGWGSIPAPGQVKV----GDEV-HTAKNIVIATGSES--SGLPGIEIDEQTVVTSTGALSLAKVPKSMVVIGAGVIGLELGSVYARLGAEVTVVEYLDAITPGMD-------AEVAKGLQRILTR 214

HSP 2 Score: 55.8398 bits (133), Expect = 8.152e-19
Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 1

Query:  505 RTTIKELIKSEESIKVITDHGEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGDGTNRMNPTPAA*WREHALQKLFLGDTLQTNYGMDPGLDLAT 852
            + T++  ++ +ES   I        A+VVL ATGR P TK L L+A+ VE+   G V+ D +  TNVP ++A+GD          A     A+ ++  G     NY + PG+   T
Sbjct:  234 KNTVRYTLRKDESAHAIE-------AEVVLVATGRKPFTKGLGLEALGVEMLPRGQVKADSHWATNVPGLYAIGDAIVGPMLAHKAEDEGMAVAEVIAGKHGHVNYDVIPGVIYTT 342

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_PSEF5 (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sthA PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.378e-18
Identity = 46/126 (36.51%), Postives = 62/126 (49.21%), Query Frame = 2

Query:  101 IYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            I K++ S  G      V ++ G G       VEV   +G      AKHI+IATGSR  R A I      I  SD  LSL   P++ +V G G I  E+ASI+ G+G  V+L+  +   L   D E+
Sbjct:   93 ISKQVASRTGYYARNRVDVFFGTGSFADEQTVEVVCANGVVEKLVAKHIIIATGSRPYRPADIDFSHPRIYDSDTILSLGHTPRKLIVYGAGVIGCEYASIFSGLGVLVELVDNRGQLLSFLDSEI 218

HSP 2 Score: 53.5286 bits (127), Expect = 1.378e-18
Identity = 24/55 (43.64%), Postives = 38/55 (69.09%), Query Frame = 1

Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I AD +L+  GR  NT +L ++ + V+V+  G +EVD+N RT VP+I+  GD
Sbjct:  256 GKKIKADALLWCNGRTGNTDKLGMENIGVKVNSRGQIEVDENYRTCVPNIYGAGD 310

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_PSEPK (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3)

HSP 1 Score: 62.003 bits (149), Expect = 1.791e-18
Identity = 45/126 (35.71%), Postives = 63/126 (50.00%), Query Frame = 2

Query:  101 IYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            I K++ S  G      V ++ G G       VEV   +G     +AKHI+IATGSR  R A I      +  SD  LSL   P++ +V G G I  E+ASI+ G+G  V+L+  +   L   D E+
Sbjct:   93 IAKQVASRTGYYARNRVDVFVGTGSFADEQTVEVVCPNGVVEKLNAKHIIIATGSRPYRPADIDFHHPRVYDSDTILSLSHTPRKLIVYGAGVIGCEYASIFSGLGVLVELVDNRGQLLSFLDSEI 218

HSP 2 Score: 51.9878 bits (123), Expect = 1.791e-18
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = 1

Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I AD +L+  GR  NT +L L+ + ++V+  G +EVD+  RT VP+I+  GD
Sbjct:  256 GKKIKADALLWCNGRTGNTDKLGLENIGIKVNSRGQIEVDEAYRTTVPNIYGAGD 310

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_PSEP1 (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sthA PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.791e-18
Identity = 45/126 (35.71%), Postives = 63/126 (50.00%), Query Frame = 2

Query:  101 IYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            I K++ S  G      V ++ G G       VEV   +G     +AKHI+IATGSR  R A I      +  SD  LSL   P++ +V G G I  E+ASI+ G+G  V+L+  +   L   D E+
Sbjct:   93 IAKQVASRTGYYARNRVDVFVGTGSFADEQTVEVVCPNGVVEKLNAKHIIIATGSRPYRPADIDFHHPRVYDSDTILSLSHTPRKLIVYGAGVIGCEYASIFSGLGVLVELVDNRGQLLSFLDSEI 218

HSP 2 Score: 51.9878 bits (123), Expect = 1.791e-18
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = 1

Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I AD +L+  GR  NT +L L+ + ++V+  G +EVD+  RT VP+I+  GD
Sbjct:  256 GKKIKADALLWCNGRTGNTDKLGLENIGIKVNSRGQIEVDEAYRTTVPNIYGAGD 310

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: MERA_STAES (Mercuric reductase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=merA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.321e-18
Identity = 40/125 (32.00%), Postives = 61/125 (48.80%), Query Frame = 2

Query:  104 YKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA--ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVAE 472
            Y  L+   G  L  GE   +    ++V   + T  S+     LIATG+      IPG      +TS  AL L+E+P+R  V+G GYIA E   ++  +G+ V L+ R E   + +D E+   + E
Sbjct:  180 YIDLIEEYGFDLIRGEASFIDDKTIQVNGQNITSKSF-----LIATGASPAVPEIPGMNEVDYLTSTSALELKEVPQRLAVIGSGYIAAELGQMFHNLGTEVTLMQRSERLFKTYDPEISEAIDE 299

HSP 2 Score: 49.2914 bits (116), Expect = 2.321e-18
Identity = 32/91 (35.16%), Postives = 55/91 (60.44%), Query Frame = 1

Query:  472 NLEGRGINLHPRTTIKELIKSEESIKVITD-HGEE--IVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGDGT 735
            +L  +G+NL    T +++ ++ +S  +  + +G+E  I AD VL ATGR PNT+ LNL++  V+  + G V  ++  +T+   I+A GD T
Sbjct:  300 SLTEQGLNLITGVTYQKVEQNGKSTSIYIEVNGQEQVIEADQVLVATGRKPNTETLNLESAGVKTGKKGEVLTNEYLQTSNNRIYAAGDVT 390

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: MERA_STAAU (Mercuric reductase OS=Staphylococcus aureus GN=merA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.321e-18
Identity = 40/125 (32.00%), Postives = 61/125 (48.80%), Query Frame = 2

Query:  104 YKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA--ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVAE 472
            Y  L+   G  L  GE   +    ++V   + T  S+     LIATG+      IPG      +TS  AL L+E+P+R  V+G GYIA E   ++  +G+ V L+ R E   + +D E+   + E
Sbjct:  180 YIDLIEEYGFDLIRGEASFIDDKTIQVNGQNITSKSF-----LIATGASPAVPEIPGMNEVDYLTSTSALELKEVPQRLAVIGSGYIAAELGQMFHNLGTEVTLMQRSERLFKTYDPEISEAIDE 299

HSP 2 Score: 49.2914 bits (116), Expect = 2.321e-18
Identity = 32/91 (35.16%), Postives = 55/91 (60.44%), Query Frame = 1

Query:  472 NLEGRGINLHPRTTIKELIKSEESIKVITD-HGEE--IVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGDGT 735
            +L  +G+NL    T +++ ++ +S  +  + +G+E  I AD VL ATGR PNT+ LNL++  V+  + G V  ++  +T+   I+A GD T
Sbjct:  300 SLTEQGLNLITGVTYQKVEQNGKSTSIYIEVNGQEQVIEADQVLVATGRKPNTETLNLESAGVKTGKKGEVLTNEYLQTSNNRIYAAGDVT 390

[Close]

BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_PSEFS (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain SBW25) GN=sthA PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.329e-18
Identity = 46/126 (36.51%), Postives = 63/126 (50.00%), Query Frame = 2

Query:  101 IYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            I K++ S  G      V L+ G G       VEV   +G      AKHI+IATGSR  R A I      I  SD  LSL   P++ ++ G G I  E+ASI+ G+G  V+L+  ++  L   D E+
Sbjct:   93 ISKQVASRTGYYARNRVDLFFGTGSFADEQTVEVVCANGVVEKLVAKHIIIATGSRPYRPADIDFHHPRIYDSDTILSLGHTPRKLIIYGAGVIGCEYASIFSGLGVLVELVDNRDQLLSFLDSEI 218

HSP 2 Score: 50.447 bits (119), Expect = 2.329e-18
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = 1

Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I AD +L+  GR  NT +L ++ + V+V+  G +EVD+N RT V +I+  GD
Sbjct:  256 GKKIKADALLWCNGRTGNTDKLGMENIGVKVNSRGQIEVDENYRTCVTNIYGAGD 310

The following BLAST results are available for this feature:

BLAST of EY659290 vs. ExPASy Swiss-Prot

BLAST of EY659290 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 178


Match Name	E-value	Identity	Description
DLDH1_PSEPU	4.820e-19	37.90	Dihydrolipoyl dehydrogenase OS=Pseudomonas putida ...	[more]
DLDH_SCHPO	8.132e-19	30.28	Dihydrolipoyl dehydrogenase, mitochondrial OS=Schi...	[more]
STHA_PSEPW	8.148e-19	36.51	Soluble pyridine nucleotide transhydrogenase OS=Ps...	[more]
DLDH_RHOCA	8.152e-19	31.76	Dihydrolipoyl dehydrogenase OS=Rhodobacter capsula...	[more]
STHA_PSEF5	1.378e-18	36.51	Soluble pyridine nucleotide transhydrogenase OS=Ps...	[more]
STHA_PSEPK	1.791e-18	35.71	Soluble pyridine nucleotide transhydrogenase OS=Ps...	[more]
STHA_PSEP1	1.791e-18	35.71	Soluble pyridine nucleotide transhydrogenase OS=Ps...	[more]
MERA_STAES	2.321e-18	32.00	Mercuric reductase OS=Staphylococcus epidermidis (...	[more]
MERA_STAAU	2.321e-18	32.00	Mercuric reductase OS=Staphylococcus aureus GN=mer...	[more]
STHA_PSEFS	2.329e-18	36.51	Soluble pyridine nucleotide transhydrogenase OS=Ps...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-008-E04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659290 ID=EY659290; Name=EY659290; organism=Citrus sinensis; type=EST; length=906bp
AGATGCAAGGAGTTATGGATGGGAGGTACATGAGATAAATGACTTCGACT
GGAAAAAACTTCTGCAAAAGAAGACAGATGAAATACTCAGACTAAATGGA
ATTTACAAGCGATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGA
GGGAAAGATAGTTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCA
CTAAGTTAAGTTACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGG
GCTCAACGTGCTCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGA
GGCATTGAGCTTGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAG
GGTACATTGCTGTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACT
GTGGATCTATTATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGA
AATGCGGGCAGTAGTTGCCGAAATCTGGAAGGGAGGGGAATTAATTTGCA
CCCGAGGACTACCATAAAGGAGTTGATTAAGTCAGAGGAGAGTATAAAAG
TTATTACAGATCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCC
ACTGGCAGGGCACCTAATACAAAAAGGTTGAATTTGAAAGCTGTTGTGGT
GGAAGTTGATCAAACAGGAGCTGTGGAGGTGGACGATAACTCACGCACCA
ACGTACCTAGTATATGGGCTGTGGGTGATGGAACTAATCGAATGAATCCT
ACCCCTGCGGCTTGATGGAGGGAACATGCTTTGCAAAAACTGTTTTTGGG
GGACACCCTGCAAACCAACTACGGGATGGACCCTGGGCTGGATTTGGCTA
CCTCCTTCTTTGGGAGGGGCCCCATGGAGGGACAGGCCTTAACCAGGAAA
TGGTGT