EY675147

Overview
NameEY675147
Unique NameEY675147
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1033
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 1.551e-52
Identity = 96/140 (68.57%), Postives = 113/140 (80.71%), Query Frame = 3 
Query:   90 NSSLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKIS 509
            N+ LE VDPEI D+IE EK RQ +G+ELI SENFTS +V++A+GS +TNKYSEG PG RYYGGNE+ID  E LC+ RAL+ F LDP +WGVNVQP SGSPANF  YTA+L+PH+RIM LDLP GGHL+HGY T   KKIS
Sbjct:   52 NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTD-TKKIS 190

HSP 2 Score: 25.7942 bits (55), Expect = 1.551e-52
Identity = 15/58 (25.86%), Postives = 31/58 (53.45%), Query Frame = 2 
Query:  500 KNIGRTSIYFESLPYKVEFQILGILITISWEEKRRWKFLGQKLIILRGGGGFPRDGEY 673
            K I   SI+FE++PY+++ +  G    I +++  +   L +  +I+ G   + R  +Y
Sbjct:  187 KKISAVSIFFETMPYRLD-ESTGY---IDYDQMEKSATLFRPKLIVAGASAYARLYDY 240
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_RABIT (Serine hydroxymethyltransferase, cytosolic OS=Oryctolagus cuniculus GN=SHMT1 PE=1 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 2.023e-52
Identity = 98/158 (62.03%), Postives = 116/158 (73.42%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRFTSRACRTRWNSKYWVY 572
            L+  D E++D+I+KE  RQ  G+ELIASENF S AV+EALGS L NKYSEG PG RYYGG E IDE+E LC+ RALQ + LDP  WGVNVQPYSGSPANFA YTA++EPH RIMGLDLP GGHLTHG+ T   K  +  + F S A +   ++ Y  Y
Sbjct:   26 LKDSDAEVYDIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEHIDELETLCQKRALQAYGLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMAYKVNPDTGYIDY 183

HSP 2 Score: 26.5646 bits (57), Expect = 2.023e-52
Identity = 11/24 (45.83%), Postives = 18/24 (75.00%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPKV 627
            P+TGYIDY++L  ++   +F PK+
Sbjct:  176 PDTGYIDYDRL--EENARLFHPKL 197
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_CAEEL (Serine hydroxymethyltransferase OS=Caenorhabditis elegans GN=mel-32 PE=1 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 5.838e-52
Identity = 94/142 (66.20%), Postives = 116/142 (81.69%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRF 524
            +E VDPE+ D+++ EK+RQ RG+ELIASENFTS AV++ALGSA+ NKYSEG PG RYYGGNEFID++E LC+ RAL+ F LDP +WGVNVQP SGSPANFA YTA++  + RIMGLDLP GGHLTHG++T   +K+S    F
Sbjct:   52 VEKVDPEVFDIMKNEKKRQRRGLELIASENFTSKAVMDALGSAMCNKYSEGYPGARYYGGNEFIDQMELLCQKRALEVFGLDPAKWGVNVQPLSGSPANFAVYTAIVGSNGRIMGLDLPDGGHLTHGFFTP-ARKVSATSEF 192

HSP 2 Score: 25.0238 bits (53), Expect = 5.838e-52
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPK 624
            P TG IDY+KL  ++   +FRPK
Sbjct:  202 PTTGLIDYDKL--EQNAMLFRPK 222
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 2.196e-51
Identity = 101/165 (61.21%), Postives = 118/165 (71.52%), Query Frame = 3 
Query:   24 FSFSVNPENGKKEKMDPVNEWG---NSSLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKIS 509
            +S S  P     EK  P   W    N+ LE  DPEI D+IE EK RQ +G+ELI SENFTS +V++A+GS +TNKYSEG PG RYYGGNE+ID  E LC+ RAL+ F LD  +WGVNVQP SGSPANF  YTA+L+ HDRIM LDLP GGHL+HGY T   KKIS
Sbjct:   28 YSMSSLPSEAVYEKERPGVTWPKQLNAPLEVGDPEIADIIELEKARQWKGLELILSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDAAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLSHGYQTD-TKKIS 191

HSP 2 Score: 24.6386 bits (52), Expect = 2.196e-51
Identity = 15/58 (25.86%), Postives = 30/58 (51.72%), Query Frame = 2 
Query:  500 KNIGRTSIYFESLPYKVEFQILGILITISWEEKRRWKFLGQKLIILRGGGGFPRDGEY 673
            K I   SI+FE++PY++  +  G    I +++  +   L +  +I+ G   + R  +Y
Sbjct:  188 KKISAVSIFFETMPYRLN-ESTGY---IDYDQLEKSATLFRPKLIVAGASAYARLYDY 241
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_HUMAN (Serine hydroxymethyltransferase, cytosolic OS=Homo sapiens GN=SHMT1 PE=1 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 2.198e-51
Identity = 97/158 (61.39%), Postives = 116/158 (73.42%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRFTSRACRTRWNSKYWVY 572
            L+  D E++++I+KE  RQ  G+ELIASENF S AV+EALGS L NKYSEG PG RYYGG EFIDE+E LC+ RALQ + LDP  WGVNVQPYSGSPANFA YTA++EPH RIMGLDLP GGHLTHG+ T   K  +  + F S   +   ++ Y  Y
Sbjct:   26 LKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINY 183

HSP 2 Score: 24.2534 bits (51), Expect = 2.198e-51
Identity = 10/24 (41.67%), Postives = 18/24 (75.00%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPKV 627
            P+TGYI+Y++L  ++   +F PK+
Sbjct:  176 PDTGYINYDQL--EENARLFHPKL 197
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_SHEEP (Serine hydroxymethyltransferase, cytosolic OS=Ovis aries GN=SHMT1 PE=1 SV=3)

HSP 1 Score: 196.052 bits (497), Expect = 1.831e-50
Identity = 97/158 (61.39%), Postives = 116/158 (73.42%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRFTSRACRTRWNSKYWVY 572
            L+  D E++++I+KE  RQ  G+ELIASENF S AV+EALGS L NKYSEG PG RYYGG EFIDE+E LC+ RALQ + LDP  WGVNVQPYSGSPANFA YTA++EPH RIMGLDLP GGHLTHG+ T   K  +  + F S   +   ++ Y  Y
Sbjct:   26 LKDNDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEVLCQKRALQVYGLDPECWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINY 183

HSP 2 Score: 23.0978 bits (48), Expect = 1.831e-50
Identity = 9/24 (37.50%), Postives = 18/24 (75.00%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPKV 627
            P+TGYI+Y++L  ++   +F P++
Sbjct:  176 PDTGYINYDQL--EENARLFHPRL 197
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_BOVIN (Serine hydroxymethyltransferase, cytosolic OS=Bos taurus GN=SHMT1 PE=2 SV=3)

HSP 1 Score: 192.971 bits (489), Expect = 1.526e-49
Identity = 96/158 (60.76%), Postives = 115/158 (72.78%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRFTSRACRTRWNSKYWVY 572
            L+  D E++++I+KE  RQ  G+ELIASENF S AV+EALGS L NKYSEG PG RYYGG EFIDE+E LC+ RALQ + LD   WGVNVQPYSGSPANFA YTA++EPH RIMGLDLP GGHLTHG+ T   K  +  + F S   +   ++ Y  Y
Sbjct:   26 LKDNDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEVLCQKRALQVYGLDSQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINY 183

HSP 2 Score: 23.0978 bits (48), Expect = 1.526e-49
Identity = 9/24 (37.50%), Postives = 18/24 (75.00%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPKV 627
            P+TGYI+Y++L  ++   +F P++
Sbjct:  176 PDTGYINYDQL--EENARLFHPRL 197
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_CAEBR (Serine hydroxymethyltransferase OS=Caenorhabditis briggsae GN=mel-32 PE=3 SV=3)

HSP 1 Score: 191.815 bits (486), Expect = 5.451e-48
Identity = 91/142 (64.08%), Postives = 114/142 (80.28%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKISGELRF 524
            +E +DPE+ ++++ EK RQ RG+ELIASENFTS AV++ALGSA+ NKYSEG PG RYYGGNEFID++E LC+ RAL+ F LDP +WGVNVQ  SGSPANFA YTA++  + RIMGLDLP GGHLTHG++T   +K+S    F
Sbjct:   56 VEKIDPEVFNIMKNEKSRQRRGLELIASENFTSKAVMDALGSAMCNKYSEGYPGARYYGGNEFIDQMEILCQKRALEVFGLDPAKWGVNVQSLSGSPANFAVYTALVGANGRIMGLDLPDGGHLTHGFFTP-ARKVSATSEF 196
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYD_SCHPO (Probable serine hydroxymethyltransferase, cytosolic OS=Schizosaccharomyces pombe GN=SPAC24C9.12c PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 2.252e-47
Identity = 85/137 (62.04%), Postives = 104/137 (75.91%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYTSGGKKIS 509
            L+  DP + +++  E  RQ   + LIASENFTS AV++ALGS ++NKYSEG PG RYYGGN+FID+IE LC+ RAL  F+LDP +WGVNVQ  SGSPAN   Y A++ PH R+MGLDLPSGGHL+HGY T   KKIS
Sbjct:   12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGGNKFIDQIETLCQERALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPPHGRLMGLDLPSGGHLSHGYQTD-TKKIS 147

HSP 2 Score: 26.9498 bits (58), Expect = 2.252e-47
Identity = 12/24 (50.00%), Postives = 17/24 (70.83%), Query Frame = 1 
Query:  556 PNTGYIDYNKLGGKKALEIFRPKV 627
            PNTG IDY+ L  +   ++FRPK+
Sbjct:  162 PNTGLIDYDML--EHDAQLFRPKI 183

HSP 3 Score: 26.5646 bits (57), Expect = 2.252e-47
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 2 
Query:  500 KNIGRTSIYFESLPYKVE 553
            K I   S YFES+PY+V+
Sbjct:  144 KKISAVSTYFESMPYRVD 161
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYC_SCHPO (Probable serine hydroxymethyltransferase, cytosolic OS=Schizosaccharomyces pombe GN=shm2 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.344e-47
Identity = 86/130 (66.15%), Postives = 104/130 (80.00%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGYYT 488
            L   DP ++ ++E EK RQ   I LIASENFTS AV++ALGS + NKYSEG PG RYYGGNEFID+ E LC++RAL+ FHLD  +WGVNVQP+SGSPAN  AY AV++PHDR+MGLDLP GGHL+HG+ T
Sbjct:   18 LAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPGARYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKPHDRLMGLDLPHGGHLSHGFST 147

HSP 2 Score: 22.3274 bits (46), Expect = 2.344e-47
Identity = 15/59 (25.42%), Postives = 27/59 (45.76%), Query Frame = 2 
Query:  497 EKNIGRTSIYFESLPYKVEFQILGILITISWEEKRRWKFLGQKLIILRGGGGFPRDGEY 673
            +K I   S YF ++PY V  +  GI   I ++   +     +  +I+ G   + R  +Y
Sbjct:  149 QKAISAVSTYFSTMPYNVNKE-TGI---IDYDSLEKAAIQFRPKVIVAGASAYARLVDY 203
The following BLAST results are available for this feature:
BLAST of EY675147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYM_ARATH1.551e-5268.57Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYC_RABIT2.023e-5262.03Serine hydroxymethyltransferase, cytosolic OS=Oryc... [more]
GLYC_CAEEL5.838e-5266.20Serine hydroxymethyltransferase OS=Caenorhabditis ... [more]
GLYN_FLAPR2.196e-5161.21Serine hydroxymethyltransferase 2, mitochondrial O... [more]
GLYC_HUMAN2.198e-5161.39Serine hydroxymethyltransferase, cytosolic OS=Homo... [more]
GLYC_SHEEP1.831e-5061.39Serine hydroxymethyltransferase, cytosolic OS=Ovis... [more]
GLYC_BOVIN1.526e-4960.76Serine hydroxymethyltransferase, cytosolic OS=Bos ... [more]
GLYC_CAEBR5.451e-4864.08Serine hydroxymethyltransferase OS=Caenorhabditis ... [more]
GLYD_SCHPO2.252e-4762.04Probable serine hydroxymethyltransferase, cytosoli... [more]
GLYC_SCHPO2.344e-4766.15Probable serine hydroxymethyltransferase, cytosoli... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-F12-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675147 ID=EY675147; Name=EY675147; organism=Citrus sinensis; type=EST; length=1033bp
CCACGCGTCCGCATTTTCCCTTTTTTTCGTTTTCAGTGAATCCAGAAAAC
GGAAAAAAAGAAAAAATGGATCCAGTGAACGAGTGGGGCAACTCATCTCT
TGAAACCGTGGACCCAGAAATTCACGACCTGATCGAGAAAGAGAAGCGCC
GACAATGCCGCGGCATCGAGCTCATCGCCTCCGAGAACTTCACCTCCTTC
GCCGTTATCGAAGCCCTCGGCAGCGCCCTCACCAACAAATACTCAGAGGG
AATGCCCGGCAACCGTTACTACGGAGGCAACGAATTCATCGACGAGATCG
AGAATCTCTGCCGGTCGAGAGCTTTACAAACTTTCCATTTGGACCCCACC
CAGTGGGGTGTCAACGTTCAGCCTTACTCCGGCTCGCCGGCCAACTTCGC
CGCTTACACGGCGGTGCTCGAGCCTCATGACCGTATCATGGGGCTGGACT
TGCCGTCCGGAGGGCATTTGACTCATGGGTATTACACTTCTGGCGGGAAA
AAAATATCGGGCGAACTTCGATTTACTTCGAGAGCTTGCCGTACAAGGTG
GAATTCCAAATACTGGGTATATTGATTACAATAAGTTGGGAGGAAAAAAG
GCGTTGGAAATTTTTAGGCCAAAAGTTGATTATTTTGCGGGGGGGAGGGG
GTTTCCCAAGGGATGGGGAATATGGCTAATTTCAAAGCCGTTGGCTAATA
AAAGGGGGGGGCCCCTTTTGCCCGAGGGAAAAGGGGGCAAAAAGACAAGG
GGCTTGGGTGGGGGCCCAAGGAAGACAGGAAAACCCCTTTTGAATAAAAG
GTACATTAAGATCACAACCCCCGATACTCTCAAAAAAACATGGGGGGGGG
GCCCATCAGGGGCGAGGGGAGGAGTATTTTTTCACCCAGGAAAGGGGGGC
CAAAAAACACAAAGGACCAAAAGAGGGGGAGAGAAACACAAAAAGAGGGG
GGGGGGGAGAATCATAAAAAGGTGTCTGTAAAACATAAAACAGACTAAGT
ACTAGCATGAGCGTTTGAATGATGAGAATACTA
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