EY675147

Overview
NameEY675147
Unique NameEY675147
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1033
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_BACLD (Serine hydroxymethyltransferase OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=glyA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.948e-29
Identity = 67/127 (52.76%), Postives = 85/127 (66.93%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            L   D ++   I+ E++RQ   IELIASENF S AV+EA GS LTNKY+EG PG RYYGG E +D  E++ R RA Q F  +     VNVQP+SG+ AN A Y  +LE  D ++G++L  GGHLTHG
Sbjct:    4 LPAQDEQVFSAIQDERKRQQSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVAEDIARDRAKQIFGAE----HVNVQPHSGAQANMAVYFTILEHGDTVLGMNLAHGGHLTHG 126
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_AGRRK (Serine hydroxymethyltransferase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=glyA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.948e-29
Identity = 72/130 (55.38%), Postives = 84/130 (64.62%), Query Frame = 3 
Query:   90 NSSLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            N SL   DPEI   I KE  RQ   IELIASEN  S AV+EA GS +TNKY+EG PG RYYGG +++D  E L   RA + F ++      NVQP SGS  N A + A+L+P D  MGLDL SGGHLTHG
Sbjct:   11 NRSLADTDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVN----FANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHG 136
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA1_RALSO (Serine hydroxymethyltransferase 1 OS=Ralstonia solanacearum GN=glyA1 PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.948e-29
Identity = 67/128 (52.34%), Postives = 86/128 (67.19%), Query Frame = 3 
Query:   96 SLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            +++ +DPE+   I++E +RQ   IELIASEN+TS AV+ A GS LTNKY+EG PG RYYGG E++D +E L   R  Q F  +      NVQP SGS AN   + A+L+P D IMG+ L  GGHLTHG
Sbjct:    8 TIDQIDPEVFAAIQQENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDVVEQLAIDRVKQLFGAE----AANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHG 131
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA1_ERWCT (Serine hydroxymethyltransferase 1 OS=Erwinia carotovora subsp. atroseptica GN=glyA1 PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.948e-29
Identity = 68/123 (55.28%), Postives = 85/123 (69.11%), Query Frame = 3 
Query:  111 DPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            D ++   +E+E  RQ   IELIASEN+TS  V++A GS LTNKY+EG PG RYYGG E++D +E L   RA   F  D      NVQP+SGS ANFA YTA+L+P D I+G++L  GGHLTHG
Sbjct:   12 DADLWQAMEQEVVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGAD----YANVQPHSGSQANFAVYTALLQPGDTILGMNLAHGGHLTHG 130
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_STAES (Serine hydroxymethyltransferase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 69/129 (53.49%), Postives = 85/129 (65.89%), Query Frame = 3 
Query:   93 SSLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            S +E  D  ++D I+KE +RQ   IELIASENF S AV+EA GS LTNKY+EG PG RYYGG E +D  E++   RA   F  +     VNVQP+SGS AN A Y   LE  D ++G++L  GGHLTHG
Sbjct:    2 SYIEKKDKVVYDAIQKEFQRQNSNIELIASENFVSQAVMEAQGSVLTNKYAEGYPGRRYYGGCEHVDVTESIAIERAKALFGAE----HVNVQPHSGSQANMAVYLVALEMGDTVLGMNLSHGGHLTHG 126
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_STAEQ (Serine hydroxymethyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 69/129 (53.49%), Postives = 85/129 (65.89%), Query Frame = 3 
Query:   93 SSLETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            S +E  D  ++D I+KE +RQ   IELIASENF S AV+EA GS LTNKY+EG PG RYYGG E +D  E++   RA   F  +     VNVQP+SGS AN A Y   LE  D ++G++L  GGHLTHG
Sbjct:    2 SYIEKKDKVVYDAIQKEFQRQNSNIELIASENFVSQAVMEAQGSVLTNKYAEGYPGRRYYGGCEHVDVTESIAIERAKALFGAE----HVNVQPHSGSQANMAVYLVALEMGDTVLGMNLSHGGHLTHG 126
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_MYCLE (Serine hydroxymethyltransferase OS=Mycobacterium leprae GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 66/127 (51.97%), Postives = 87/127 (68.50%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            L  VDP+I +L+ KE  RQ   +E+IASENF   +V++A GS LTNKY+EG+PG RYY G E +D +EN+ R RA   F  D      NVQP+SG+ AN A   A++ P +R++GLDL +GGHLTHG
Sbjct:    5 LAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRRYYDGCEHVDVVENIARDRAKALFGAD----FANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHG 127
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_MYCLB (Serine hydroxymethyltransferase OS=Mycobacterium leprae (strain Br4923) GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 66/127 (51.97%), Postives = 87/127 (68.50%), Query Frame = 3 
Query:   99 LETVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHG 479
            L  VDP+I +L+ KE  RQ   +E+IASENF   +V++A GS LTNKY+EG+PG RYY G E +D +EN+ R RA   F  D      NVQP+SG+ AN A   A++ P +R++GLDL +GGHLTHG
Sbjct:    5 LAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRRYYDGCEHVDVVENIARDRAKALFGAD----FANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHG 127
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_MYCH7 (Serine hydroxymethyltransferase OS=Mycoplasma hyopneumoniae (strain 7448) GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 67/124 (54.03%), Postives = 86/124 (69.35%), Query Frame = 3 
Query:  111 DPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGY 482
            D +I +LI  E +RQ   IELIASEN+ S  VI A G++L+NKY EG PG RYYGG  FID+IE +   R  + F ++      NVQPYSGS AN A + A+L+P D+I+GLDL +GGHL+HGY
Sbjct:    9 DQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKRYYGGCTFIDQIEKIAIERVKKLFKIE----YANVQPYSGSSANAAVFAALLKPGDKILGLDLNAGGHLSHGY 128
[Close]
BLAST of EY675147 vs. ExPASy Swiss-Prot
Match: GLYA_MYCH2 (Serine hydroxymethyltransferase OS=Mycoplasma hyopneumoniae (strain 232) GN=glyA PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.544e-29
Identity = 67/124 (54.03%), Postives = 86/124 (69.35%), Query Frame = 3 
Query:  111 DPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQTFHLDPTQWGVNVQPYSGSPANFAAYTAVLEPHDRIMGLDLPSGGHLTHGY 482
            D +I +LI  E +RQ   IELIASEN+ S  VI A G++L+NKY EG PG RYYGG  FID+IE +   R  + F ++      NVQPYSGS AN A + A+L+P D+I+GLDL +GGHL+HGY
Sbjct:    9 DQQISELINLESKRQNSQIELIASENYASEDVILANGTSLSNKYGEGYPGKRYYGGCTFIDQIEKIAIERVKKLFKIE----YANVQPYSGSSANAAVFAALLKPGDKILGLDLNAGGHLSHGY 128
The following BLAST results are available for this feature:
BLAST of EY675147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_BACLD1.948e-2952.76Serine hydroxymethyltransferase OS=Bacillus lichen... [more]
GLYA_AGRRK1.948e-2955.38Serine hydroxymethyltransferase OS=Agrobacterium r... [more]
GLYA1_RALSO1.948e-2952.34Serine hydroxymethyltransferase 1 OS=Ralstonia sol... [more]
GLYA1_ERWCT1.948e-2955.28Serine hydroxymethyltransferase 1 OS=Erwinia carot... [more]
GLYA_STAES2.544e-2953.49Serine hydroxymethyltransferase OS=Staphylococcus ... [more]
GLYA_STAEQ2.544e-2953.49Serine hydroxymethyltransferase OS=Staphylococcus ... [more]
GLYA_MYCLE2.544e-2951.97Serine hydroxymethyltransferase OS=Mycobacterium l... [more]
GLYA_MYCLB2.544e-2951.97Serine hydroxymethyltransferase OS=Mycobacterium l... [more]
GLYA_MYCH72.544e-2954.03Serine hydroxymethyltransferase OS=Mycoplasma hyop... [more]
GLYA_MYCH22.544e-2954.03Serine hydroxymethyltransferase OS=Mycoplasma hyop... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-F12-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675147 ID=EY675147; Name=EY675147; organism=Citrus sinensis; type=EST; length=1033bp
CCACGCGTCCGCATTTTCCCTTTTTTTCGTTTTCAGTGAATCCAGAAAAC
GGAAAAAAAGAAAAAATGGATCCAGTGAACGAGTGGGGCAACTCATCTCT
TGAAACCGTGGACCCAGAAATTCACGACCTGATCGAGAAAGAGAAGCGCC
GACAATGCCGCGGCATCGAGCTCATCGCCTCCGAGAACTTCACCTCCTTC
GCCGTTATCGAAGCCCTCGGCAGCGCCCTCACCAACAAATACTCAGAGGG
AATGCCCGGCAACCGTTACTACGGAGGCAACGAATTCATCGACGAGATCG
AGAATCTCTGCCGGTCGAGAGCTTTACAAACTTTCCATTTGGACCCCACC
CAGTGGGGTGTCAACGTTCAGCCTTACTCCGGCTCGCCGGCCAACTTCGC
CGCTTACACGGCGGTGCTCGAGCCTCATGACCGTATCATGGGGCTGGACT
TGCCGTCCGGAGGGCATTTGACTCATGGGTATTACACTTCTGGCGGGAAA
AAAATATCGGGCGAACTTCGATTTACTTCGAGAGCTTGCCGTACAAGGTG
GAATTCCAAATACTGGGTATATTGATTACAATAAGTTGGGAGGAAAAAAG
GCGTTGGAAATTTTTAGGCCAAAAGTTGATTATTTTGCGGGGGGGAGGGG
GTTTCCCAAGGGATGGGGAATATGGCTAATTTCAAAGCCGTTGGCTAATA
AAAGGGGGGGGCCCCTTTTGCCCGAGGGAAAAGGGGGCAAAAAGACAAGG
GGCTTGGGTGGGGGCCCAAGGAAGACAGGAAAACCCCTTTTGAATAAAAG
GTACATTAAGATCACAACCCCCGATACTCTCAAAAAAACATGGGGGGGGG
GCCCATCAGGGGCGAGGGGAGGAGTATTTTTTCACCCAGGAAAGGGGGGC
CAAAAAACACAAAGGACCAAAAGAGGGGGAGAGAAACACAAAAAGAGGGG
GGGGGGGAGAATCATAAAAAGGTGTCTGTAAAACATAAAACAGACTAAGT
ACTAGCATGAGCGTTTGAATGATGAGAATACTA
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