EY675140 | Citrus Genome Database

Overview


Name	EY675140
Unique Name	EY675140
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	974

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CEPT1_MOUSE (Choline/ethanolaminephosphotransferase 1 OS=Mus musculus GN=Cept1 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.569e-15
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFW---VISAVPFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWW 429
            Q+ DA+DGKQARRTNSSSPLGELFDHGCD+L+  F  +        G +  W +         F+ A W+ Y + TL   +++  TE  + I + H    I G  +W
Sbjct:  130 QSLDAIDGKQARRTNSSSPLGELFDHGCDSLSTVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTYVSGTLRFGIID-VTEVQIFIIIMHLLAVIGGPPFW 235

HSP 2 Score: 21.9422 bits (45), Expect = 2.569e-15
Identity = 9/25 (36.00%), Postives = 11/25 (44.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P      P W + A    LF+YQ
Sbjct:  106 YCPTATEQAPLWAYIACACGLFIYQ 130

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CEPT1_HUMAN (Choline/ethanolaminephosphotransferase 1 OS=Homo sapiens GN=CEPT1 PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.569e-15
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFW---VISAVPFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWW 429
            Q+ DA+DGKQARRTNSSSPLGELFDHGCD+L+  F  +        G +  W +         F+ A W+ Y + TL   +++  TE  + I + H    I G  +W
Sbjct:  130 QSLDAIDGKQARRTNSSSPLGELFDHGCDSLSTVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTYVSGTLRFGIID-VTEVQIFIIIMHLLAVIGGPPFW 235

HSP 2 Score: 21.9422 bits (45), Expect = 2.569e-15
Identity = 9/25 (36.00%), Postives = 11/25 (44.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P      P W + A    LF+YQ
Sbjct:  106 YCPTATEQAPLWAYIACACGLFIYQ 130

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CPT1_YEAST (Cholinephosphotransferase 1 OS=Saccharomyces cerevisiae GN=CPT1 PE=1 SV=3)

HSP 1 Score: 69.3218 bits (168), Expect = 7.336e-15
Identity = 46/141 (32.62%), Postives = 66/141 (46.81%), Query Frame = 1

Query:  112 LCQTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFWVISAV--PFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWWAQNFGNSMPFLSWVPFINAIPTNRAVLYLMIAF 528
            L QTFDA DG  ARRT    PLGELFDH  D++      +   S    G      +   A+   F+ +TWE Y T+ L L    GP EG++++ +      I G +     +   +   SW  F+  + T    ++LM AF
Sbjct:   91 LYQTFDACDGMHARRTGQQGPLGELFDHCIDSINTTLSMIPVCSMTGMGYTYMTIFSQFAILCSFYLSTWEEYHTHKLYLAEFCGPVEGIIVLCISFIAVGIYGPQ---TIWHTKVAQFSWQDFVFDVET----VHLMYAF 224

HSP 2 Score: 32.7278 bits (73), Expect = 7.336e-15
Identity = 12/25 (48.00%), Postives = 16/25 (64.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P  D   PRW +F++ + LFLYQ
Sbjct:   69 YDPYFDQESPRWTYFSYAIGLFLYQ 93

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CEPT1_CHICK (Choline/ethanolaminephosphotransferase 1 OS=Gallus gallus GN=CEPT1 PE=2 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 1.607e-14
Identity = 41/107 (38.32%), Postives = 58/107 (54.21%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFW---VISAVPFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWW 429
            Q+ D +DGKQARRTNSS+PLGELFDHGCD+L+  F  +        G +  W +         F+ A W+ Y + TL   +++  TE  + I + H    I G  +W
Sbjct:  130 QSLDTIDGKQARRTNSSTPLGELFDHGCDSLSTVFVVLGTCIAVQLGTNPDWMFFCCFAGTFMFYCAHWQTYVSGTLRFGIID-VTEVQIFIIIMHLLAVIGGPPFW 235

HSP 2 Score: 21.9422 bits (45), Expect = 1.607e-14
Identity = 9/25 (36.00%), Postives = 11/25 (44.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P      P W + A    LF+YQ
Sbjct:  106 YCPTATEQAPPWAYIACACGLFIYQ 130

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CHPT1_HUMAN (Cholinephosphotransferase 1 OS=Homo sapiens GN=CHPT1 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.089e-14
Identity = 45/107 (42.06%), Postives = 60/107 (56.07%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFWVISAV---PFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWW 429
            Q+ DA+DGKQARRTNS SPLGELFDHGCD+L+  F A+     A  G    WF+  S +    F+ A W+ Y +  L    V+  TE  + + +    +A  GA  W
Sbjct:  108 QSLDAIDGKQARRTNSCSPLGELFDHGCDSLSTVFMAVGASIAARLGTYPDWFFFCSFIGMFVFYCAHWQTYVSGMLRFGKVD-VTEIQIALVIVFVLSAFGGATMW 213

HSP 2 Score: 21.9422 bits (45), Expect = 2.089e-14
Identity = 9/25 (36.00%), Postives = 11/25 (44.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P      P W +    L LF+YQ
Sbjct:   84 YCPTATEEAPYWTYLLCALGLFIYQ 108

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CHPT1_XENLA (Cholinephosphotransferase 1 OS=Xenopus laevis GN=chpt1 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.582e-14
Identity = 42/107 (39.25%), Postives = 60/107 (56.07%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFW---VISAVPFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWW 429
            Q+ DA+DGKQARRTNSSSPLGE+FDHGCD+++  F  +   +    G    W +    +    F+ A W+ Y   TL   +++  TE  + + V    TA+ G E W
Sbjct:  108 QSLDAIDGKQARRTNSSSPLGEMFDHGCDSISIVFVNLGTIAAVRLGTLPGWMFYCCFVGMFMFYCAQWQTYVCGTLKFGIID-VTELQISVTVMFLMTAVCGPELW 213

HSP 2 Score: 20.7866 bits (42), Expect = 4.582e-14
Identity = 8/25 (32.00%), Postives = 11/25 (44.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P      P W +    + LF+YQ
Sbjct:   84 YCPTATEGAPFWTYLLCAIGLFVYQ 108

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CHPT1_RAT (Cholinephosphotransferase 1 OS=Rattus norvegicus GN=Chpt1 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.305e-13
Identity = 51/154 (33.12%), Postives = 70/154 (45.45%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFWVISAV---PFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWWAQNFGNSMPFLSWVPFINAIPTNRAVLYLMIAFGVIPTVYFHVSNVY 570
            Q+ DA+DGKQARRTNS SPLGELFDHGCD+L+  F A+        G    W +  S V    F+ A W+ Y +  L    V+  TE  + + +    +   GA  W                   IP     L ++   GV+  + F  SN +
Sbjct:  108 QSLDAIDGKQARRTNSCSPLGELFDHGCDSLSTVFMAIGASIAVRLGTHPDWLFFCSFVGMFMFYCAHWQTYVSGVLRFGRVD-VTEIQVALVIVFLLSTFGGAMMWDY----------------TIPILEIKLKILPVLGVVGGLIFSCSNYF 244

HSP 2 Score: 22.7126 bits (47), Expect = 1.305e-13
Identity = 9/25 (36.00%), Postives = 12/25 (48.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P +    P W +    L LF+YQ
Sbjct:   84 YCPTVTEEAPYWTYLLCALGLFIYQ 108

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CHPT1_MOUSE (Cholinephosphotransferase 1 OS=Mus musculus GN=Chpt1 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.305e-13
Identity = 51/154 (33.12%), Postives = 70/154 (45.45%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFWVISAV---PFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWWAQNFGNSMPFLSWVPFINAIPTNRAVLYLMIAFGVIPTVYFHVSNVY 570
            Q+ DA+DGKQARRTNS SPLGELFDHGCD+L+  F A+        G    W +  S V    F+ A W+ Y +  L    V+  TE  + + +    +   GA  W                   IP     L ++   GV+  + F  SN +
Sbjct:  108 QSLDAIDGKQARRTNSCSPLGELFDHGCDSLSTVFMAIGASIAVRLGTHPDWLFFCSFVGMFMFYCAHWQTYVSGVLRFGRVD-VTEIQVALVIVFMLSTFGGATMWDY----------------TIPILEIKLKIVPVLGVVGGLIFSCSNYF 244

HSP 2 Score: 22.7126 bits (47), Expect = 1.305e-13
Identity = 9/25 (36.00%), Postives = 12/25 (48.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P +    P W +    L LF+YQ
Sbjct:   84 YCPTVTEEAPYWTYLLCALGLFIYQ 108

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: CHPT1_BOVIN (Cholinephosphotransferase 1 OS=Bos taurus GN=CHPT1 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.694e-13
Identity = 50/154 (32.47%), Postives = 69/154 (44.81%), Query Frame = 1

Query:  118 QTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGSTAMCGRDTFWFWVISAV---PFFGATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWWAQNFGNSMPFLSWVPFINAIPTNRAVLYLMIAFGVIPTVYFHVSNVY 570
            Q+ DA+DGKQARRTNS SPLGELFDHGCD+L+  F A+        G    W +  S +    F+ A W+ Y +  L    V+  TE  + + +    +   GA  W                   IP     L ++   GV+    F  SN +
Sbjct:  108 QSLDAIDGKQARRTNSCSPLGELFDHGCDSLSTVFMAVGASIAVRLGTHPDWLFFCSFIGMFMFYCAHWQTYVSGVLRFGKVD-VTEIQIALVIVFVLSTFGGATMWDY----------------TIPILEIKLKILPVLGVVGGAIFSCSNYF 244

HSP 2 Score: 22.7126 bits (47), Expect = 1.694e-13
Identity = 9/25 (36.00%), Postives = 12/25 (48.00%), Query Frame = 3

Query:   12 YSPCLDTAPPRWVHFAHGLLLFLYQ 86
            Y P +    P W +    L LF+YQ
Sbjct:   84 YCPTVTEEAPYWTYLLCALGLFIYQ 108

[Close]

BLAST of EY675140 vs. ExPASy Swiss-Prot
Match: SELI_BOVIN (Ethanolaminephosphotransferase 1 OS=Bos taurus GN=SELI PE=2 SV=3)

HSP 1 Score: 73.1738 bits (178), Expect = 2.611e-12
Identity = 46/116 (39.66%), Postives = 60/116 (51.72%), Query Frame = 1

Query:  112 LCQTFDAVDGKQARRTNSSSPLGELFDHGCDALACAFEAMAFGS---TAMCGRDTFWFWVISAVPFFG---ATWEHYFTNTLILPVVNGPTEGLMLIYVGHFFTAIVGAEWWAQNF 441
            +  T D VDGKQARRTNSS+PLGELFDHG D+ +C +  +   S       G   F  +++  V  F    + WE Y T  L LP   G     + I   +  TA+VG E W + F
Sbjct:   99 MAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSSGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPW--GYDISQVTISFVYIVTAVVGVEAWYEPF 212

The following BLAST results are available for this feature:

BLAST of EY675140 vs. ExPASy Swiss-Prot

BLAST of EY675140 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24


Match Name	E-value	Identity	Description
CEPT1_MOUSE	2.569e-15	40.19	Choline/ethanolaminephosphotransferase 1 OS=Mus mu...	[more]
CEPT1_HUMAN	2.569e-15	40.19	Choline/ethanolaminephosphotransferase 1 OS=Homo s...	[more]
CPT1_YEAST	7.336e-15	32.62	Cholinephosphotransferase 1 OS=Saccharomyces cerev...	[more]
CEPT1_CHICK	1.607e-14	38.32	Choline/ethanolaminephosphotransferase 1 OS=Gallus...	[more]
CHPT1_HUMAN	2.089e-14	42.06	Cholinephosphotransferase 1 OS=Homo sapiens GN=CHP...	[more]
CHPT1_XENLA	4.582e-14	39.25	Cholinephosphotransferase 1 OS=Xenopus laevis GN=c...	[more]
CHPT1_RAT	1.305e-13	33.12	Cholinephosphotransferase 1 OS=Rattus norvegicus G...	[more]
CHPT1_MOUSE	1.305e-13	33.12	Cholinephosphotransferase 1 OS=Mus musculus GN=Chp...	[more]
CHPT1_BOVIN	1.694e-13	32.47	Cholinephosphotransferase 1 OS=Bos taurus GN=CHPT1...	[more]
SELI_BOVIN	2.611e-12	39.66	Ethanolaminephosphotransferase 1 OS=Bos taurus GN=...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-006-F05-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY675140 ID=EY675140; Name=EY675140; organism=Citrus sinensis; type=EST; length=974bp
TTGGCTATATATATTCCCCTTGTTTGGATACAGCTCCACCTAGATGGGTT
CATTTTGCACATGGCTTGCTTCTATTTTTATATCAGGTTCCGTTCTACAA
CCAAAATGCAACTTTGTCAGACTTTTGATGCTGTTGATGGGAAGCAAGCA
CGACGGACAAATTCTTCCAGTCCACTGGGTGAACTTTTTGACCATGGTTG
TGATGCGCTAGCCTGTGCGTTTGAAGCCATGGCTTTTGGAAGCACTGCTA
TGTGTGGAAGAGATACTTTCTGGTTCTGGGTAATTTCAGCTGTTCCATTT
TTTGGAGCAACATGGGAACACTATTTTACCAATACCCTTATTCTTCCGGT
GGTCAATGGACCTACTGAGGGTCTTATGCTGATCTATGTGGGCCATTTCT
TTACAGCTATAGTTGGTGCTGAGTGGTGGGCGCAAAATTTTGGGAATTCT
ATGCCCTTCTTGAGTTGGGTGCCATTTATTAATGCAATCCCCACAAACAG
AGCAGTGTTATATTTAATGATCGCCTTTGGTGTTATCCCTACAGTATACT
TTCATGTGTCCAATGTTTATAAAGGTTGTCCAGTCAAAAAACGGAAGCAT
TTTACGGGGCATTAGCAATGCTTTACCCCTTTGTGGGACTCCTTGGAAGA
AGTGCTTTGCTGGGGATTATCTTTCCCCGGGCTGAAATTATTCGGAAATA
TCCCCATTTTCGTTTTTTTTGGGGAAAAGGAATTGGATTTTGGGTTTCCT
CCCGGGGAAAAAAAAATTTCGGGGTCCCTTTTGGGGAAAGAGCCCCCAGG
GGAATGGAAAAAACAAAAAAATGGGGCCGGGGCCCCCGGTTGGAATCCTT
CCCCTTTTTCCCCGAAACCAAAAAGGGCACTTTATCGCGGGAAAAAAAAA
AAAAAAGAGAAGGGAAAAATAACCTTTTTTAATGTTTGATAAAAAAATAT
TCTTGGGGGGGGTGTTTCTGGCGC