EY675056

Overview
NameEY675056
Unique NameEY675056
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length992
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_GOSHI (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Gossypium hirsutum GN=RBCS PE=3 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 1.571e-20
Identity = 42/70 (60.00%), Postives = 58/70 (82.86%), Query Frame = 2 
Query:  431 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            L++G+V+R++ S P YYDGRYWTMWKLPM+GCTD+ QVL+E+   +KEYP++F+RIIGF+    V+ ISF
Sbjct:  105 LEEGFVHRKYSSLPTYYDGRYWTMWKLPMFGCTDSAQVLEELENCKKEYPNAFIRIIGFDNVRQVQCISF 174
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_AEGTA (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Aegilops tauschii GN=RBCS PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.571e-20
Identity = 42/67 (62.69%), Postives = 53/67 (79.10%), Query Frame = 2 
Query:  440 GWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            G+++REH+ SPGYYDGRYWTMWKLPM+GC   TQV+ EV EV+KEYP  + RIIGF+    V+ +SF
Sbjct:   95 GFIFREHNVSPGYYDGRYWTMWKLPMFGCPAPTQVIPEVEEVRKEYPDPYCRIIGFDNMRQVQSVSF 161

HSP 2 Score: 71.2478 bits (173), Expect = 1.021e-11
Identity = 29/42 (69.05%), Postives = 34/42 (80.95%), Query Frame = 3 
Query:   27 CYMVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPC 152
            C  VWP  G+KKFETLSYLPPLS E LLK++ YL+RS W+PC
Sbjct:   47 CMQVWPIEGIKKFETLSYLPPLSTEGLLKQVDYLIRSKWVPC 88
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS1_PEA (Ribulose bisphosphate carboxylase small chain, chloroplastic (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.501e-20
Identity = 45/70 (64.29%), Postives = 52/70 (74.29%), Query Frame = 2 
Query:  431 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            L KG+VY EH+ SP YYDGRYWTMWKLPM+G TD  QV+KEV EV   YP +FVR+IGF     V+ ISF
Sbjct:   59 LLKGFVYGEHNKSPRYYDGRYWTMWKLPMFGTTDPAQVVKEVDEVVAAYPEAFVRVIGFNNVRQVQCISF 128

HSP 2 Score: 73.9442 bits (180), Expect = 1.575e-12
Identity = 30/42 (71.43%), Postives = 34/42 (80.95%), Query Frame = 3 
Query:   27 CYMVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPC 152
            C  VWPP G KKFETLSYLPPL+ + LLKE+ YLLR GW+PC
Sbjct:   13 CMQVWPPIGKKKFETLSYLPPLTRDQLLKEVEYLLRKGWVPC 54
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_SILPR (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Silene pratensis GN=RBCS PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 7.798e-20
Identity = 43/65 (66.15%), Postives = 52/65 (80.00%), Query Frame = 2 
Query:  446 VYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            VYRE++ SPGYYDGRYWTMWKLPM+GCTDA+QV  EV E +  YP + VRIIGF+ +  V+ ISF
Sbjct:  107 VYRENNKSPGYYDGRYWTMWKLPMFGCTDASQVAAEVVECKNAYPDAHVRIIGFDNKRQVQCISF 171

HSP 2 Score: 73.1738 bits (178), Expect = 2.687e-12
Identity = 31/42 (73.81%), Postives = 35/42 (83.33%), Query Frame = 3 
Query:   27 CYMVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPC 152
            C  VWPP  LKKFETLSYLP LS+E+LLKEI+YLL  GW+PC
Sbjct:   56 CMQVWPPRNLKKFETLSYLPTLSEESLLKEINYLLIKGWVPC 97
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS1_SPIOL (Ribulose bisphosphate carboxylase small chain OS=Spinacia oleracea GN=RBCS PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 7.798e-20
Identity = 44/70 (62.86%), Postives = 53/70 (75.71%), Query Frame = 2 
Query:  431 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ G+VYREH  SPGYYDGRYWTMWKLPM+G TD  QV+ EV EV+K  P +FVR IGF  +  V+ ISF
Sbjct:   46 VKDGFVYREHDKSPGYYDGRYWTMWKLPMFGGTDPAQVVNEVEEVKKAPPDAFVRFIGFNDKREVQCISF 115
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_MAIZE (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Zea mays GN=RBCS PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.737e-19
Identity = 42/73 (57.53%), Postives = 53/73 (72.60%), Query Frame = 2 
Query:  440 GWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISFPCWPAP 658
            G+VYRE+ +SP YYDGRYWTMWKLPM+GC DATQV KE+ E  K YP +F R+IGF+     + +SF  +  P
Sbjct:   95 GFVYRENSTSPCYYDGRYWTMWKLPMFGCNDATQVYKELQEAIKSYPDAFHRVIGFDNIKQTQCVSFIAYKPP 167

HSP 2 Score: 70.8626 bits (172), Expect = 1.333e-11
Identity = 30/42 (71.43%), Postives = 34/42 (80.95%), Query Frame = 3 
Query:   27 CYMVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPC 152
            C  VWP  G KKFETLSYLPPLS + LLK++ YLLR+GWIPC
Sbjct:   47 CMQVWPAYGNKKFETLSYLPPLSTDDLLKQVDYLLRNGWIPC 88
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_PINTH (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Pinus thunbergii GN=RBCS PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 5.055e-19
Identity = 42/77 (54.55%), Postives = 57/77 (74.03%), Query Frame = 2 
Query:  419 WVA*LQ---KGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            WV  L+   +G + R+++ SPGYYDGRYW MWKLPM+GCT+A+QV+ EV E  K YP +F+R+IGF+    V+ ISF
Sbjct:   89 WVPCLEFDLEGSISRKYNRSPGYYDGRYWVMWKLPMFGCTEASQVINEVRECAKAYPKAFIRVIGFDNVRQVQCISF 165
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_LARLA (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Larix laricina GN=RBCS PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 5.055e-19
Identity = 40/69 (57.97%), Postives = 53/69 (76.81%), Query Frame = 2 
Query:  434 QKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            + G + R + +SPGYYDGRYW MWKLPM+GCT+A+QVL EV E  K YP++F+R+IGF+    V+ ISF
Sbjct:  113 EDGEIKRVYGNSPGYYDGRYWVMWKLPMFGCTEASQVLNEVNECAKAYPNAFIRVIGFDNVRQVQCISF 181
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_SACHY (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Saccharum hybrid GN=RBCS PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.179e-17
Identity = 41/77 (53.25%), Postives = 54/77 (70.13%), Query Frame = 2 
Query:  419 WVA*LQ---KGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            WV  L+   +G+VYRE+ +SP YYDGRYWTMWKLPM+GCTDA+QV KE+ E    YP     I+GF+     +W++F
Sbjct:   84 WVPCLEFSKEGFVYRENSTSPCYYDGRYWTMWKLPMFGCTDASQVYKELQEAIASYP-ELRAILGFDNIRQTQWLTF 159

HSP 2 Score: 68.9366 bits (167), Expect = 5.067e-11
Identity = 28/42 (66.67%), Postives = 33/42 (78.57%), Query Frame = 3 
Query:   27 CYMVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPC 152
            C  VWP  G KKFETLSYLPPL+ E LLK++ YLLR+ W+PC
Sbjct:   46 CMQVWPAYGNKKFETLSYLPPLTQEQLLKQVDYLLRNNWVPC 87
[Close]
BLAST of EY675056 vs. ExPASy Swiss-Prot
Match: RBS_EUGGR (Ribulose bisphosphate carboxylase small chains, chloroplastic OS=Euglena gracilis GN=RBCS1 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct:  197 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 255

HSP 2 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct:  341 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 399

HSP 3 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct:  628 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 686

HSP 4 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct:  771 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 829

HSP 5 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct: 1059 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 1117

HSP 6 Score: 82.4185 bits (202), Expect = 4.428e-15
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct: 1203 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 1261

HSP 7 Score: 80.4925 bits (197), Expect = 1.683e-14
Identity = 32/59 (54.24%), Postives = 44/59 (74.58%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD  YWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  F+    V+ ISF
Sbjct:  484 TAAGYYDNHYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISF 542

HSP 8 Score: 78.1814 bits (191), Expect = 8.351e-14
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 2 
Query:  464 SSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFEQQAPVEWISF 640
            ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR + F+    V+ ISF
Sbjct:  916 TAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVR-LAFDSVKQVQVISF 973
The following BLAST results are available for this feature:
BLAST of EY675056 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 95
Match NameE-valueIdentityDescription
RBS_GOSHI1.571e-2060.00Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_AEGTA1.571e-2062.69Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS1_PEA3.501e-2064.29Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_SILPR7.798e-2066.15Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS1_SPIOL7.798e-2062.86Ribulose bisphosphate carboxylase small chain OS=S... [more]
RBS_MAIZE1.737e-1957.53Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_PINTH5.055e-1954.55Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_LARLA5.055e-1957.97Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_SACHY6.179e-1753.25Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_EUGGR4.428e-1555.93Ribulose bisphosphate carboxylase small chains, ch... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-005-D08-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675056 ID=EY675056; Name=EY675056; organism=Citrus sinensis; type=EST; length=992bp
TTTATTAATTTTCCTGTGAGCTTGTATGTTACATGGTGTGGCCACCAACT
GGCTTGAAGAAGTTCGAGACTCTCTCATACCTCCCGCCACTGTCCGATGA
AGCTTTGCTCAAGGAAATCAGTTACCTTCTCCGCTCCGGCTGGATTCCCT
GCTTGGAATTCGAGTTGGAGGTCATTTCTCTCTCTCTCTCTCTCTCTCTC
TGTCTCTCTTAATATATACTATTATTTTGACGGAACCTTATCTATGTGCT
TTAGGAGAAAAATTACACATGTCATGAATCTATTGGTCAGAGATATCCAT
TCAATATCAAAACCTGCATTAACAATTGCTTACTTAAAATCTAAGCTTTT
GGGTTAAGCGGATTTTATCAGGCAAATGGGTGGTGATATTTGATTTTTTT
TTTTTTTGTTTTTTCTTTTGGGTGGCGTGACTGCAGAAAGGATGGGTGTA
CCGTGAGCACCACAGCTCACCAGGATACTACGATGGACGCTACTGGACAA
TGTGGAAACTGCCCATGTATGGGTGCACAGATGCAACCCAGGTTTTGAAG
GAGGTTGGGGAGGTCCAAAAGGAATACCCACATTCTTTCGTCCGAATTAT
CGGATTTGAACAACAAGCGCCAGTGGAGTGGATCAGTTTTCCTTGCTGGC
CAGCCCCCAGGCGTCCAAGGTTCCCAATTTAATTGTGGTAACCCCCCAGG
CCATATTGGTTGATTATTATTATGGGTAACCCCCCAAAAAGGATCTTTCA
AATCATGGGGGGTTCGGGTTTGGGTTTAATTTGTGAATTAAGGGCGGGGT
TGGCGGCGGGCCCAAAACTTTTTTGGGTTTTCCCCCCCCACCTTGGGGTT
TTTTTTTTTTTTTTTTTATTTTTTTAAAAAAATTTAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGCGGCCCC
CCCCCCTAAGGAGGGAATAACAAACCACGCTCTTTACAACGT
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