EY674899 | Citrus Genome Database

Overview


Name	EY674899
Unique Name	EY674899
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1000

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHIC_SECCE (Basic endochitinase C OS=Secale cereale GN=rscc PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 3.404e-13
Identity = 27/51 (52.94%), Postives = 31/51 (60.78%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S  +PC   K YYG+G IQLS N+NYGPAG  IG D L  P  VA +
Sbjct:  119 YCTPSAQWPCAPGKRYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATD 169

HSP 2 Score: 31.187 bits (69), Expect = 3.404e-13
Identity = 21/64 (32.81%), Postives = 25/64 (39.06%), Query Frame = 1

Query:  397 IAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTV 525
            ++FKTALWFW        S  +V+                 GFG     ING LEC  G    V
Sbjct:  172 VSFKTALWFWMTAQAPKPSSHAVITGKWSPSGADRAAGRAPGFGVITNIINGGLECGHGQDSRV 235

HSP 3 Score: 28.1054 bits (61), Expect = 3.404e-13
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  525 SSRIRYFSECCNQLGVSTGDNLTC 596
            + RI ++   C+ LGV  GDNL C
Sbjct:  236 ADRIGFYKRYCDILGVGYGDNLDC 259

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 4.339e-13
Identity = 27/51 (52.94%), Postives = 34/51 (66.67%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S  +PC   K Y+G+G IQLS+N+NYGPAG  IG D LN P  VA++
Sbjct:  180 YCVPSAEWPCAPDKKYFGRGPIQLSYNYNYGPAGEAIGEDLLNNPELVASD 230

HSP 2 Score: 31.187 bits (69), Expect = 4.339e-13
Identity = 18/60 (30.00%), Postives = 24/60 (40.00%), Query Frame = 1

Query:  394 VIAFKTALWFW---------------------TNSVQSVMNQGFGASIQAINGELECDGG 510
            V++FKTALWFW                     +  + +    G+G     ING LEC  G
Sbjct:  232 VVSFKTALWFWMTPQSPKPSCHDVITGQWTPSSGDIAAGRVPGYGVITNIINGGLECGFG 291

HSP 3 Score: 26.1794 bits (56), Expect = 4.339e-13
Identity = 9/24 (37.50%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  525 SSRIRYFSECCNQLGVSTGDNLTC 596
            ++RI ++   C+ LG+  G NL C
Sbjct:  297 ANRIGFYQRYCDVLGIGYGSNLDC 320

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 4.346e-13
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S  +PC   + Y+G+G IQ+S N+NYGP G  IG D LN P  VA +
Sbjct:  169 YCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATD 219

HSP 2 Score: 33.113 bits (74), Expect = 4.346e-13
Identity = 23/70 (32.86%), Postives = 28/70 (40.00%), Query Frame = 1

Query:  394 VIAFKTALWFW-------------------TNSVQSVMNQ--GFGASIQAINGELECDGGNPCTVHLVLG 540
            VI+FK+ALWFW                    +S     N+  GFG     ING LEC  G    V   +G
Sbjct:  221 VISFKSALWFWMTPQSPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIG 290

HSP 3 Score: 29.261 bits (64), Expect = 4.346e-13
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C+ LGVS GDNL C
Sbjct:  288 RIGFYRRYCSILGVSPGDNLDC 309

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI3_SOLTU (Endochitinase 3 (Fragment) OS=Solanum tuberosum GN=CHTB3 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.351e-13
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANEQL 397
            Y   S+ +PC   + Y+G+G IQ+S N+NYGP G  IG D LN P  VA + +
Sbjct:  163 YCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDSV 215

HSP 2 Score: 31.9574 bits (71), Expect = 4.351e-13
Identity = 22/71 (30.99%), Postives = 29/71 (40.85%), Query Frame = 1

Query:  391 AVIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTVHLVLG 540
            +VI+FK+A+WFW        S   V+                 GFG     ING LEC  G+   V   +G
Sbjct:  214 SVISFKSAIWFWMTPQSPKPSCHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIG 284

HSP 3 Score: 28.8758 bits (63), Expect = 4.351e-13
Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C  LGVS GDNL C
Sbjct:  282 RIGFYRRYCGILGVSPGDNLDC 303

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI1_ORYSJ (Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht1 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 5.622e-13
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S+ +PC + K YYG+G IQ+S+N+NYGPAG  IG + L+ P  VA++
Sbjct:  175 YCVQSSQWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGSNLLSNPDLVASD 225

HSP 2 Score: 30.4166 bits (67), Expect = 5.622e-13
Identity = 19/61 (31.15%), Postives = 24/61 (39.34%), Query Frame = 1

Query:  391 AVIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGG 510
            A ++FKTA WFW        S  +VM                 G+G     ING +EC  G
Sbjct:  226 ATVSFKTAFWFWMTPQSPKPSCHAVMTGQWTPNGNDQAAGRVPGYGVVTNIINGGVECGHG 286

HSP 3 Score: 27.7202 bits (60), Expect = 5.622e-13
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  525 SSRIRYFSECCNQLGVSTGDNLTC 596
            + RI ++   C+ LGVS G NL C
Sbjct:  292 ADRIGFYKRYCDMLGVSYGANLDC 315

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI2_SOLTU (Endochitinase 2 (Fragment) OS=Solanum tuberosum GN=CHTB2 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.628e-13
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANEQL 397
            Y   S  +PC   + Y+G+G IQ+S N+NYGP G  IG D LN P  VA + +
Sbjct:  161 YCTPSNQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDSI 213

HSP 2 Score: 31.5722 bits (70), Expect = 5.628e-13
Identity = 21/71 (29.58%), Postives = 29/71 (40.85%), Query Frame = 1

Query:  391 AVIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTVHLVLG 540
            ++I+FK+A+WFW        S   V+                 GFG     ING LEC  G+   V   +G
Sbjct:  212 SIISFKSAIWFWMTPQSPKPSCHDVITGRWQPSGTDQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIG 282

HSP 3 Score: 28.8758 bits (63), Expect = 5.628e-13
Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C  LGVS GDNL C
Sbjct:  280 RIGFYRRYCGILGVSPGDNLDC 301

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI3_TOBAC (Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.212e-12
Identity = 24/49 (48.98%), Postives = 32/49 (65.31%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVA 385
            Y   S+ +PC   + YYG+G IQ+S+N+NYGP G  IG + LN P  VA
Sbjct:  179 YCVQSSQWPCAPGQKYYGRGPIQISYNYNYGPCGRAIGQNLLNNPDLVA 227

HSP 2 Score: 31.9574 bits (71), Expect = 1.212e-12
Identity = 21/71 (29.58%), Postives = 29/71 (40.85%), Query Frame = 1

Query:  391 AVIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTVHLVLG 540
            AV++FK+A+WFW        S   V+                 G+G     ING LEC  G+   V   +G
Sbjct:  230 AVVSFKSAIWFWMTAQSPKPSCHDVITGRWTPSAADRAANRLPGYGVITNIINGGLECGHGSDARVQDRIG 300

HSP 3 Score: 28.4906 bits (62), Expect = 1.212e-12
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C+ LGVS GDN+ C
Sbjct:  298 RIGFYRRYCSILGVSPGDNIDC 319

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 2.027e-12
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S  +PC   + Y+G+G IQ+S N+NYGP G  IG D LN P  VA +
Sbjct:  174 YCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATD 224

HSP 2 Score: 30.8018 bits (68), Expect = 2.027e-12
Identity = 23/70 (32.86%), Postives = 27/70 (38.57%), Query Frame = 1

Query:  394 VIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTVHLVLG 540
            VI+FK+ALWFW        S   V+                 GFG     ING LEC  G    V   +G
Sbjct:  226 VISFKSALWFWMTPQSPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIG 295

HSP 3 Score: 29.261 bits (64), Expect = 2.027e-12
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C+ LGVS GDNL C
Sbjct:  293 RIGFYRRYCSILGVSPGDNLDC 314

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHIC_SOLLC (Basic 30 kDa endochitinase OS=Solanum lycopersicum GN=CHI9 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.030e-12
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S+ +PC   + Y+G+G IQ+S N+NYGP G  IG D LN P  VA +
Sbjct:  167 YCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATD 217

HSP 2 Score: 31.5722 bits (70), Expect = 2.030e-12
Identity = 22/70 (31.43%), Postives = 28/70 (40.00%), Query Frame = 1

Query:  394 VIAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTVHLVLG 540
            VI+FK+A+WFW        S   V+                 GFG     ING LEC  G+   V   +G
Sbjct:  219 VISFKSAIWFWMTPQSPKPSCHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIG 288

HSP 3 Score: 27.335 bits (59), Expect = 2.030e-12
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 3

Query:  531 RIRYFSECCNQLGVSTGDNLTC 596
            RI ++   C  LGVS G+NL C
Sbjct:  286 RIGFYRRYCGILGVSPGENLDC 307

[Close]

BLAST of EY674899 vs. ExPASy Swiss-Prot
Match: CHI1_HORVU (26 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=4)

HSP 1 Score: 57.3806 bits (137), Expect = 2.625e-12
Identity = 27/51 (52.94%), Postives = 31/51 (60.78%), Query Frame = 2

Query:  239 YDESSTLYPCNSSKGYYGQGQIQLSWNFNYGPAGNRIGFDELNAPATVANE 391
            Y   S  +PC   K YYG+G IQLS N+NYGPAG  IG D L  P  VA +
Sbjct:  171 YCTPSAQWPCAPGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATD 221

HSP 2 Score: 29.261 bits (64), Expect = 2.625e-12
Identity = 18/64 (28.12%), Postives = 25/64 (39.06%), Query Frame = 1

Query:  397 IAFKTALWFWTN------SVQSVMN---------------QGFGASIQAINGELECDGGNPCTV 525
            ++FKTA+WFW        S  +V+                 GFG     +NG +EC  G    V
Sbjct:  224 VSFKTAMWFWMTAQAPKPSSHAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRV 287

HSP 3 Score: 26.1794 bits (56), Expect = 2.625e-12
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  525 SSRIRYFSECCNQLGVSTGDNLTC 596
            + RI ++   C+ LGV  G+NL C
Sbjct:  288 ADRIGFYKRYCDILGVGYGNNLDC 311

The following BLAST results are available for this feature:

BLAST of EY674899 vs. ExPASy Swiss-Prot

BLAST of EY674899 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 39


Match Name	E-value	Identity	Description
CHIC_SECCE	3.404e-13	52.94	Basic endochitinase C OS=Secale cereale GN=rscc PE...	[more]
CHI9_ORYSJ	4.339e-13	52.94	Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht...	[more]
CHI2_TOBAC	4.346e-13	47.06	Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1...	[more]
CHI3_SOLTU	4.351e-13	45.28	Endochitinase 3 (Fragment) OS=Solanum tuberosum GN...	[more]
CHI1_ORYSJ	5.622e-13	49.02	Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht...	[more]
CHI2_SOLTU	5.628e-13	45.28	Endochitinase 2 (Fragment) OS=Solanum tuberosum GN...	[more]
CHI3_TOBAC	1.212e-12	48.98	Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3...	[more]
CHI1_TOBAC	2.027e-12	47.06	Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1...	[more]
CHIC_SOLLC	2.030e-12	47.06	Basic 30 kDa endochitinase OS=Solanum lycopersicum...	[more]
CHI1_HORVU	2.625e-12	52.94	26 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-401-002-H03-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY674899 ID=EY674899; Name=EY674899; organism=Citrus sinensis; type=EST; length=1000bp
CCCACGCGTCCGGTAAAACACAATAAATACACAAGCTGTCAGAACAATTA
CAAAAAGAAAGTCAACAAAAAATAAATGAAGGAGATATATAAATAGATTT
TGTGGGTAGTAGTTGCCTGGTGGATTGGTAGTTGTTATAATTATGTACAT
TTCATGATTGCATTCCAAAAGTCATCATTCATCTTTGCATGTGTTCCACA
TTTATCATGCTTGTCTACCATGTCCGCAATAGGACTATTATGATGAATCA
AGCACTCTATATCCATGCAATTCAAGCAAAGGTTACTACGGCCAGGGACA
AATTCAACTATCTTGGAATTTCAACTACGGACCTGCCGGAAACAGAATTG
GCTTTGACGAATTAAACGCGCCGGCAACTGTTGCCAATGAGCAGTTATAG
CATTCAAGACTGCTTTATGGTTCTGGACAAACAGTGTTCAATCTGTAATG
AACCAAGGATTTGGGGCAAGCATTCAGGCCATTAATGGTGAACTTGAATG
TGATGGTGGAAACCCTTGCACTGTTCATCTCGTATTAGGTACTTCTCTGA
GTGCTGCAACCAATTGGGAGTTTCTACTGGAGATAATCTCACTTGTTAAT
ATCATCATAAAAACTGTAATGACCCAAAAAAAAAAAAAAAAATTACCAGG
CTCTCAATAAATAACCAATTTATATAAATAAAAAAATTCTTTTACCTGGC
AAAAAAAAAAAAAAAAGGGGCGCCCGCTCTAAAAAGGATTCCAGCTTTAA
TTTCCCCGGGGCATGGCAAACCCCAAAACCTCTTTTTAAAGGGGGTCCCC
AAAATTTTCATTTTCCTGGGGCCCCCCTTTTTTTAAACCCCTCGGGGGGG
GGGGGAAAAACCCCTGGGGCGTTTTCCCCCAATTTTATATCCCCCCTTGT
GCCACCACCACTCCCCCCCCTTTTTCCCCCCCCCCGGGGGGGGGAAAAAA
AAAAAAAAAAAAAAAAAAAGCGCCCCCCCCCACCCCCCCATTTTCTCTCC