EY674782 | Citrus Genome Database

Overview


Name	EY674782
Unique Name	EY674782
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1038

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPCB_HUMAN (cAMP-dependent protein kinase catalytic subunit beta OS=Homo sapiens GN=PRKACB PE=1 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 1.028e-22
Identity = 54/120 (45.00%), Postives = 74/120 (61.67%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS 396
            + P+ L++D   ++Q+ DF F K + G RT+T+CG P+YLAPEI+  KG+  A DWWALGVLIY M  G  PF  + +  I I  KI  G++  P + S +  DLL  LL VD   R G+
Sbjct:  168 LKPENLLIDHQGYIQVTDFGFAKRVKG-RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPF--FADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGN 284

HSP 2 Score: 23.8682 bits (50), Expect = 1.028e-22
Identity = 8/14 (57.14%), Postives = 8/14 (57.14%), Query Frame = 2

Query:  416 KTHPWFKDVDWKGI 457
            KTH WF   DW  I
Sbjct:  293 KTHKWFATTDWIAI 306

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPCB_CRIGR (cAMP-dependent protein kinase catalytic subunit beta OS=Cricetulus griseus GN=PRKACB PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 1.028e-22
Identity = 54/120 (45.00%), Postives = 74/120 (61.67%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS 396
            + P+ L++D   ++Q+ DF F K + G RT+T+CG P+YLAPEI+  KG+  A DWWALGVLIY M  G  PF  + +  I I  KI  G++  P + S +  DLL  LL VD   R G+
Sbjct:  168 LKPENLLIDHQGYIQVTDFGFAKRVKG-RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPF--FADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGN 284

HSP 2 Score: 23.8682 bits (50), Expect = 1.028e-22
Identity = 8/14 (57.14%), Postives = 8/14 (57.14%), Query Frame = 2

Query:  416 KTHPWFKDVDWKGI 457
            KTH WF   DW  I
Sbjct:  293 KTHKWFATTDWIAI 306

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPCB_BOVIN (cAMP-dependent protein kinase catalytic subunit beta OS=Bos taurus GN=PRKACB PE=1 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 1.028e-22
Identity = 54/120 (45.00%), Postives = 74/120 (61.67%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS 396
            + P+ L++D   ++Q+ DF F K + G RT+T+CG P+YLAPEI+  KG+  A DWWALGVLIY M  G  PF  + +  I I  KI  G++  P + S +  DLL  LL VD   R G+
Sbjct:  168 LKPENLLIDHQGYIQVTDFGFAKRVKG-RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPF--FADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGN 284

HSP 2 Score: 23.8682 bits (50), Expect = 1.028e-22
Identity = 8/14 (57.14%), Postives = 8/14 (57.14%), Query Frame = 2

Query:  416 KTHPWFKDVDWKGI 457
            KTH WF   DW  I
Sbjct:  293 KTHKWFATTDWIAI 306

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPC_ASCSU (cAMP-dependent protein kinase catalytic subunit OS=Ascaris suum GN=CAPK PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.338e-22
Identity = 61/131 (46.56%), Postives = 81/131 (61.83%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEID-IVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQ--GPTSVKN 420
            + P+ LML+K  HL++ DF F K +  +RT+T+CG P+YLAPE++  KGH  A DWW+LGVLIY M+ G  PF   R   +D I  KI  G+L   ++    A DL+ KLL VD   RLG+Q  G   V N
Sbjct:  153 LKPENLMLNKEGHLKMTDFGFAKEVI-DRTWTMCGTPEYLAPEVIGNKGHDTAVDWWSLGVLIYEMMIGIPPF---RGKTLDEIYEKIILGKLRFTRSFDLFAKDLVKKLLQVDRTQRLGNQKDGAADVMN 279

HSP 2 Score: 23.8682 bits (50), Expect = 1.338e-22
Identity = 6/12 (50.00%), Postives = 8/12 (66.67%), Query Frame = 2

Query:  422 HPWFKDVDWKGI 457
            H WF D+DW  +
Sbjct:  280 HKWFTDIDWDDV 291

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPB_SCHPO (cAMP-dependent protein kinase catalytic subunit OS=Schizosaccharomyces pombe GN=pka1 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.922e-22
Identity = 51/120 (42.50%), Postives = 77/120 (64.17%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS 396
            + P+ L+LD+  HL++VDF F K +S +   T+CG PDYLAPEI+  K +  AADWW+LG+LI+ ML G  PF  + E+ + +   I +G+++ P   S  ++DLL+ LL  D   R G+
Sbjct:  325 LKPENLLLDRFGHLKIVDFGFAKRVSTSNCCTLCGTPDYLAPEIISLKPYNKAADWWSLGILIFEMLAGYPPF--YSENPMKLYENILEGKVNYPSYFSPASIDLLSHLLQRDITCRYGN 442

HSP 2 Score: 25.7942 bits (55), Expect = 2.922e-22
Identity = 7/12 (58.33%), Postives = 9/12 (75.00%), Query Frame = 2

Query:  422 HPWFKDVDWKGI 457
            HPWF+D+ W  I
Sbjct:  453 HPWFRDISWDKI 464

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPK19_ARATH (Serine/threonine-protein kinase AtPK19 OS=Arabidopsis thaliana GN=ATPK19 PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 4.954e-22
Identity = 57/138 (41.30%), Postives = 86/138 (62.32%), Query Frame = 1

Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGLSGN-RTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGPTSVK 417
            + ++G++   + P+ +++D   H+ L DF   K    N R+ ++CG  +Y+APEIV+GKGH  AADWW++G+L+Y ML G+ PF     S+  I  KI K ++ LPQ LS+EA  LL  LL  +   RLGS GP+  +
Sbjct:  254 LHEKGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRGKGHDKAADWWSVGILLYEMLTGKPPF---LGSKGKIQQKIVKDKIKLPQFLSNEAHALLKGLLQKEPERRLGS-GPSGAE 387

HSP 2 Score: 24.2534 bits (51), Expect = 4.954e-22
Identity = 7/12 (58.33%), Postives = 9/12 (75.00%), Query Frame = 2

Query:  416 KTHPWFKDVDWK 451
            K H WFK ++WK
Sbjct:  390 KKHKWFKAINWK 401

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPCG_HUMAN (cAMP-dependent protein kinase catalytic subunit gamma OS=Homo sapiens GN=PRKACG PE=1 SV=3)

HSP 1 Score: 105.531 bits (262), Expect = 4.984e-22
Identity = 58/130 (44.62%), Postives = 78/130 (60.00%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS--QGPTSVKN 420
            + P+ L++D+  +LQ+ DF F K + G RT+T+CG P+YLAPEI+  KG+  A DWWALGVLIY M  G  PF  + +  I I  KI  G++  P  LS +   LL  LL VD   R G+   G   +KN
Sbjct:  168 LKPENLLIDQQGYLQVTDFGFAKRVKG-RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAVGFPPF--YADQPIQIYEKIVSGRVRFPSKLSSDLKHLLRSLLQVDLTKRFGNLRNGVGDIKN 294

HSP 2 Score: 20.7866 bits (42), Expect = 4.984e-22
Identity = 7/16 (43.75%), Postives = 7/16 (43.75%), Query Frame = 2

Query:  416 KTHPWFKDVDWKGIAE 463
            K H WF    W  I E
Sbjct:  293 KNHKWFATTSWIAIYE 308

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPK1_ARATH (Serine/threonine-protein kinase AtPK1/AtPK6 OS=Arabidopsis thaliana GN=ATPK1 PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.091e-21
Identity = 54/131 (41.22%), Postives = 82/131 (62.60%), Query Frame = 1

Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGLSGN-RTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS 396
            + ++G++   + P+ +++D   H+ L DF   K    N R+ ++CG  +Y+APEIV+GKGH  AADWW++G+L+Y ML G+ PF     S+  I  KI K ++ LPQ LS+EA  +L  LL  +   RLGS
Sbjct:  248 LHEKGIMHRDLKPENILMDTDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRGKGHDKAADWWSVGILLYEMLTGKPPF---LGSKGKIQQKIVKDKIKLPQFLSNEAHAILKGLLQKEPERRLGS 375

HSP 2 Score: 24.6386 bits (52), Expect = 1.091e-21
Identity = 7/12 (58.33%), Postives = 9/12 (75.00%), Query Frame = 2

Query:  416 KTHPWFKDVDWK 451
            K H WFK ++WK
Sbjct:  384 KQHKWFKGINWK 395

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: GAD8_SCHPO (Serine/threonine-protein kinase gad8 OS=Schizosaccharomyces pombe GN=gad8 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.839e-21
Identity = 54/132 (40.91%), Postives = 75/132 (56.82%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGK--GLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGPTSVKNSP 426
            + P+ ++LD T H+ L DF   K      +RT T CG P+YLAPE++ G G+    DWW LGVL+Y M+ G  PF  + E+  ++  KI +  L  P N+  +A DLL+ LL      RLGS G   +KN P
Sbjct:  354 LKPENILLDYTGHIALCDFGLCKLNMAKTDRTNTFCGTPEYLAPELLLGHGYTKVVDWWTLGVLLYEMITGLPPF--YDENINEMYRKILQDPLRFPDNIDEKAKDLLSGLLTRAPEKRLGSGGAQEIKNHP 483

HSP 2 Score: 26.9498 bits (58), Expect = 1.839e-21
Identity = 13/46 (28.26%), Postives = 22/46 (47.83%), Query Frame = 2

Query:  416 KTHPWFKDVDWKGI-AESTSPCSHMKKMSPHKSNTWNRSFLEGFPL 550
            K HP+F D+DWK + A+   P       S   ++ ++  F    P+
Sbjct:  480 KNHPFFDDIDWKKLCAKKIQPPFKPSVESAIDTSNFDSEFTSEIPM 525

[Close]

BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KAPC_DROME (cAMP-dependent protein kinase catalytic subunit OS=Drosophila melanogaster GN=Pka-C1 PE=1 SV=3)

HSP 1 Score: 103.605 bits (257), Expect = 1.978e-21
Identity = 55/130 (42.31%), Postives = 77/130 (59.23%), Query Frame = 1

Query:   37 VSPDVLMLDKTLHLQLVDFRFGKGLSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGS--QGPTSVKN 420
            + P+ L++D   +L++ DF F K + G RT+T+CG P+YLAPEI+  KG+  A DWWALGVL+Y M  G  PF  + +  I I  KI  G++  P +   +  DLL  LL VD   R G+   G   +KN
Sbjct:  170 LKPENLLIDSQGYLKVTDFGFAKRVKG-RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPPF--FADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKN 296

The following BLAST results are available for this feature:

BLAST of EY674782 vs. ExPASy Swiss-Prot

BLAST of EY674782 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 375


Match Name	E-value	Identity	Description
KAPCB_HUMAN	1.028e-22	45.00	cAMP-dependent protein kinase catalytic subunit be...	[more]
KAPCB_CRIGR	1.028e-22	45.00	cAMP-dependent protein kinase catalytic subunit be...	[more]
KAPCB_BOVIN	1.028e-22	45.00	cAMP-dependent protein kinase catalytic subunit be...	[more]
KAPC_ASCSU	1.338e-22	46.56	cAMP-dependent protein kinase catalytic subunit OS...	[more]
KAPB_SCHPO	2.922e-22	42.50	cAMP-dependent protein kinase catalytic subunit OS...	[more]
KPK19_ARATH	4.954e-22	41.30	Serine/threonine-protein kinase AtPK19 OS=Arabidop...	[more]
KAPCG_HUMAN	4.984e-22	44.62	cAMP-dependent protein kinase catalytic subunit ga...	[more]
KPK1_ARATH	1.091e-21	41.22	Serine/threonine-protein kinase AtPK1/AtPK6 OS=Ara...	[more]
GAD8_SCHPO	1.839e-21	40.91	Serine/threonine-protein kinase gad8 OS=Schizosacc...	[more]
KAPC_DROME	1.978e-21	42.31	cAMP-dependent protein kinase catalytic subunit OS...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-401-001-D03-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY674782 ID=EY674782; Name=EY674782; organism=Citrus sinensis; type=EST; length=1038bp
CCACGCGTCCGCAAGAGAGGTGTTCTCCCCTGAGGTGTGTCTCCTGATGT
TCTAATGTTGGACAAAACCCTACATTTACAGTTAGTAGACTTTCGATTCG
GGAAAGGCTTATCTGGCAACAGAACATTCACAATTTGTGGAATCCCAGAC
TATTTAGCTCCAGAGATAGTTCAGGGAAAAGGCCATGGTCTCGCTGCTGA
CTGGTGGGCATTGGGAGTCCTAATTTATTTCATGTTACAAGGTGAAATGC
CATTTGGATCTTGGAGAGAGAGTGAAATAGATATTGTTGCAAAGATTGCA
AAAGGGCAGCTAAGTCTTCCACAGAATTTAAGCCATGAAGCTGTTGATCT
CCTTACAAAGTTGCTCGTAGTTGATGAAAATACAAGACTTGGTAGCCAAG
GTCCCACTTCTGTCAAAAACTCACCCATGGTTCAAAGATGTTGATTGGAA
AGGGATTGCTGAATCTACTTCTCCTTGTTCCCACATGAAAAAAATGTCTC
CGCATAAGTCAAACACCTGGAATAGGTCATTTTTGGAGGGATTTCCCCTG
GTTTTTTCAAGCTTCCTCCGCCCTTCGAAATGTTGAAAGAACTCAAATGG
TTCCTGAAAGGGGTTTGATTAACTGGGAAGGAGTTTAAAATTGGGTATCC
TTTATTTTTTGGGTTTCTGGTTTTCCCCTATTTAATTCCTTGGCCAAAAA
ATTTTCTTTTCCCTTGGGTTTTCCTTCCCCCTGCCTGGGTCCCTTGGGCC
TTAATTTTGGAAAGTTTTTGTAAAAAAAACCCCCACCTTTTTTCCAAAAC
TTTCAAAATATGGGGGTAAAACACAAAATTATAAATAAAAAACAAAAAAA
TTATAAAATTTTGGCGGGGTTGTCGCCCCCCCCACGGGGCCCCCATTAAA
TTTTTTGGCCCCACAAAAAAAACCCCCAATTATAAATTTATTTTTGGTTT
TTTTTGTGGTGGGCCCCCCACAATATGTTATAAGAGAATAGAGGAAGACG
CGGAAAAGGAGGGGGGGGGGTGTGGTGTGGGGTGGTGT