EY674161 | Citrus Genome Database

Overview


Name	EY674161
Unique Name	EY674161
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1029

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_RICB8 (Lipid-A-disaccharide synthase OS=Rickettsia bellii (strain OSU 85-389) GN=lpxB PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.387e-12
Identity = 21/60 (35.00%), Postives = 44/60 (73.33%), Query Frame = 1

Query:  121 KSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQLR 300
            +SLFP+ +I +MG +E++PHI++ +  + +T+E  +  +P  ++T+DS GF++R+  ++R
Sbjct:   47 ESLFPISEINLMGFFEVIPHIFRIKKLINKTVEDIIDNKPDILITIDSPGFTYRVAAKVR 106

HSP 2 Score: 27.335 bits (59), Expect = 1.387e-12
Identity = 13/24 (54.17%), Postives = 14/24 (58.33%), Query Frame = 2

Query:  341 ERLNGPAHFHYVAPSFWAWKGGEA 412
            ERL      H VAPS WA+K G A
Sbjct:  107 ERLPELKMIHIVAPSVWAYKEGRA 130

HSP 3 Score: 26.1794 bits (56), Expect = 1.387e-12
Identity = 22/89 (24.72%), Postives = 38/89 (42.70%), Query Frame = 1

Query:  607 KNKYSVPSGAMIISLLPGSRLQEVARMLPIFAKTMELLKDSFSRS**QSFMWDLIGIVKILHLD-------LLEMARAFYIESWKMLQS 852
            + +  +     ++ +  GSR  E+ R LPIF   +E + D   +         L+ I  + + D        LE  R  YI S++ L+S
Sbjct:  178 RQELEIDEDTKVLCVTLGSRKGEILRHLPIFIPAIEKVYDDHKKK--------LMVIFPLANPDHERIIKPFLEKVRFNYIFSYERLKS 258

HSP 4 Score: 23.483 bits (49), Expect = 1.387e-12
Identity = 9/28 (32.14%), Postives = 15/28 (53.57%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVE 530
            +  +LP E       GL   ++GHP++E
Sbjct:  140 LFALLPFEPPYFTKVGLDCRYIGHPIME 167

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_DECAR (Lipid-A-disaccharide synthase OS=Dechloromonas aromatica (strain RCB) GN=lpxB PE=3 SV=1)

HSP 1 Score: 37.3502 bits (85), Expect = 2.956e-12
Identity = 21/64 (32.81%), Postives = 35/64 (54.69%), Query Frame = 1

Query:  112 QGMKSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALL-FRPHAVVTVDSKGFSFRLLKQLR 300
            QG  S +PME ++VMG W+ L H Y+    ++  ++  LL  +P   + VD+  F+  L   L+
Sbjct:   47 QGFDSWWPMEKLSVMGYWDALKH-YREIAGIRRQLKKRLLDLKPDIFIGVDAPDFNLGLETNLK 109

HSP 2 Score: 34.6538 bits (78), Expect = 2.956e-12
Identity = 15/30 (50.00%), Postives = 20/30 (66.67%), Query Frame = 2

Query:  353 GPAHFHYVAPSFWAWKGGEARLKNLAAFVD 442
            G    HYV+PS WAW+GG  R+K +A  V+
Sbjct:  112 GVRTIHYVSPSIWAWRGG--RVKKIAKAVN 139

HSP 3 Score: 32.3426 bits (72), Expect = 2.956e-12
Identity = 14/40 (35.00%), Postives = 25/40 (62.50%), Query Frame = 1

Query:  607 KNKYSVPSGAMIISLLPGSRLQEVARMLPIFAKTMELLKD 726
            + K S+P    I ++LPGSR  E+A M   F +T +++++
Sbjct:  180 REKLSLPRDYPIFAMLPGSRQGELAMMAETFVETAKIIRE 219

HSP 4 Score: 27.335 bits (59), Expect = 2.956e-12
Identity = 10/29 (34.48%), Postives = 17/29 (58.62%), Query Frame = 3

Query:  444 RILCILPNEEAICRLNGLAATFVGHPVVE 530
            R+L + P E A+     +  T+VGHP+ +
Sbjct:  140 RVLALFPMEPALYEKERVPVTYVGHPLAD 168

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_BURTA (Lipid-A-disaccharide synthase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=lpxB PE=3 SV=1)

HSP 1 Score: 35.4242 bits (80), Expect = 6.293e-12
Identity = 22/59 (37.29%), Postives = 35/59 (59.32%), Query Frame = 1

Query:  130 FPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRP-HAVVTVDSKGFSFRLLKQLRG 303
            +PME +AV G  E L HI +  +R++  ++  LL  P  A + +D+  F+F L + LRG
Sbjct:   57 WPMEKLAVRGYVEALKHIPEI-LRIRGELKRQLLAEPPDAFIGIDAPDFNFGLEQALRG 114

HSP 2 Score: 33.8834 bits (76), Expect = 6.293e-12
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVED 533
            +LC+ P E  +    G+AATFVGHP+ ++
Sbjct:  145 MLCLFPFEPELLEKAGVAATFVGHPLADE 173

HSP 3 Score: 33.113 bits (74), Expect = 6.293e-12
Identity = 13/25 (52.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  368 HYVAPSFWAWKGGEARLKNLAAFVD 442
            H+V PS WAW+GG  R+K +   VD
Sbjct:  121 HFVCPSIWAWRGG--RIKKIVKAVD 143

HSP 4 Score: 28.1054 bits (61), Expect = 6.293e-12
Identity = 12/34 (35.29%), Postives = 20/34 (58.82%), Query Frame = 1

Query:  622 VPSGAMIISLLPGSRLQEVARMLPIFAKTMELLK 723
            +P    +I++LPGSR  E+  + P F   M L++
Sbjct:  189 LPDSGPVIAVLPGSRRSEIELIGPTFFDAMALMQ 222

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_RICCK (Lipid-A-disaccharide synthase OS=Rickettsia canadensis (strain McKiel) GN=lpxB PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.297e-12
Identity = 24/60 (40.00%), Postives = 41/60 (68.33%), Query Frame = 1

Query:  121 KSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQLR 300
            KSLFP+  I ++G  E+LPHI K +  + +T+E  +  +   ++T+DS GF++R+ KQ+R
Sbjct:   47 KSLFPISAINLIGFVEILPHILKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKQVR 106

HSP 2 Score: 25.7942 bits (55), Expect = 6.297e-12
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 2

Query:  368 HYVAPSFWAWKGGEA 412
            H VAPS WA+K G A
Sbjct:  116 HIVAPSVWAYKEGRA 130

HSP 3 Score: 25.7942 bits (55), Expect = 6.297e-12
Identity = 10/41 (24.39%), Postives = 23/41 (56.10%), Query Frame = 1

Query:  607 KNKYSVPSGAMIISLLPGSRLQEVARMLPIFAKTMELLKDS 729
            + ++ +     ++ +  GSR  E+ R LP+F  ++E + +S
Sbjct:  178 RKEFKIDENEKVLCVTLGSRRGEILRHLPVFIASIEEIFES 218

HSP 4 Score: 24.2534 bits (51), Expect = 6.297e-12
Identity = 9/28 (32.14%), Postives = 15/28 (53.57%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVE 530
            +  +LP E       GL   ++GHP++E
Sbjct:  140 LFAVLPFESPYFTKVGLDCRYIGHPIME 167

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_ALCBS (Lipid-A-disaccharide synthase OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=lpxB PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 6.300e-12
Identity = 24/64 (37.50%), Postives = 38/64 (59.38%), Query Frame = 1

Query:  106 SEQGMKSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQL 297
            ++ G+ SLFPME ++VMGI E+L H+ +     K  +   L  RP  VV +DS  F+  + ++L
Sbjct:   48 AQAGLTSLFPMEKLSVMGITEVLSHLPELLRLRKSLVRFLLEQRPDVVVGIDSPDFTLPIARRL 111

HSP 2 Score: 33.113 bits (74), Expect = 6.300e-12
Identity = 17/39 (43.59%), Postives = 23/39 (58.97%), Query Frame = 2

Query:  326 ARYSHERLNGPAHFHYVAPSFWAWKGGEARLKNLAAFVD 442
            AR  H+R  G    HYV+PS WAW+ G  R+K +   +D
Sbjct:  108 ARRLHDR--GLKTVHYVSPSVWAWRQG--RIKGIKKSID 142

HSP 3 Score: 28.1054 bits (61), Expect = 6.300e-12
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  634 AMIISLLPGSRLQEVARMLPIFAKTMELL 720
            A I+++LPGSR  EV +++P F   M  L
Sbjct:  192 ARILAVLPGSRGGEVGQLMPAFLDAMVAL 220

HSP 4 Score: 22.3274 bits (46), Expect = 6.300e-12
Identity = 9/28 (32.14%), Postives = 15/28 (53.57%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVE 530
            +L +LP E      + +   FVGHP+ +
Sbjct:  144 MLTLLPFEARFYEEHDVPVAFVGHPLAD 171

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_RICAH (Lipid-A-disaccharide synthase OS=Rickettsia akari (strain Hartford) GN=lpxB PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.216e-11
Identity = 24/60 (40.00%), Postives = 41/60 (68.33%), Query Frame = 1

Query:  121 KSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQLR 300
            KSLFP+  I +MG  E+LPHI+  +  + +T++  +  +   ++T+DS GF++R+ KQLR
Sbjct:   47 KSLFPITCINLMGFVEILPHIFNLKKLIDKTVQDIINSQADLLITIDSPGFTYRVAKQLR 106

HSP 2 Score: 26.1794 bits (56), Expect = 2.216e-11
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 2

Query:  368 HYVAPSFWAWKGGEA 412
            H VAPS WA+K G A
Sbjct:  116 HIVAPSVWAYKDGRA 130

HSP 3 Score: 24.2534 bits (51), Expect = 2.216e-11
Identity = 8/36 (22.22%), Postives = 20/36 (55.56%), Query Frame = 1

Query:  607 KNKYSVPSGAMIISLLPGSRLQEVARMLPIFAKTME 714
            + ++ +     ++ +  GSR  E+ + LP+F  ++E
Sbjct:  178 REEFKIDKNERVLCVTLGSRQGEIRKHLPVFISSIE 213

HSP 4 Score: 23.483 bits (49), Expect = 2.216e-11
Identity = 9/28 (32.14%), Postives = 15/28 (53.57%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVE 530
            +  +LP E       GL   ++GHP++E
Sbjct:  140 LFALLPFEPPYFTKVGLDCRYIGHPIME 167

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_CHRSD (Lipid-A-disaccharide synthase OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=lpxB PE=3 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 6.072e-11
Identity = 27/72 (37.50%), Postives = 46/72 (63.89%), Query Frame = 1

Query:   94 PRVRSEQGMKSLFPMEDIAVMGIWELLPH---IYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQLR 300
            PR+ +E GM+SL+P+E ++VMG+ E+L H   + K R  L+     AL ++P  ++ +D+  F+  L +QLR
Sbjct:   38 PRMLAE-GMQSLYPLETLSVMGLVEVLKHLPGLIKVRRHLRRD---ALAWQPDVMIGIDAPDFNLGLERQLR 105

HSP 2 Score: 34.6538 bits (78), Expect = 6.072e-11
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 2

Query:  344 RLNGPAHFHYVAPSFWAWKGGEARLKNLAAFVDPHL 451
            R  G    HYV+PS WAW+ G  R+K +A  VD  L
Sbjct:  105 RATGMRTVHYVSPSVWAWRQG--RVKTIARSVDAML 138

HSP 3 Score: 23.8682 bits (50), Expect = 6.072e-11
Identity = 10/29 (34.48%), Postives = 16/29 (55.17%), Query Frame = 3

Query:  447 ILCILPNEEAICRLNGLAATFVGHPVVED 533
            +L  LP E A    + +   FVGHP+ ++
Sbjct:  137 MLTFLPFEAAFYARHQVPVAFVGHPLADE 165

HSP 4 Score: 23.0978 bits (48), Expect = 6.072e-11
Identity = 11/33 (33.33%), Postives = 20/33 (60.61%), Query Frame = 1

Query:  628 SGAMIISLLPGSRLQEVARMLPIFAKTMELLKD 726
            S A ++++LPGSR  E+  + P F  +   L++
Sbjct:  183 STAPLLAVLPGSRGNEIRFLGPTFLDSAVWLRE 215

[Close]

BLAST of EY674161 vs. ExPASy Swiss-Prot
Match: LPXB_NITHX (Lipid-A-disaccharide synthase OS=Nitrobacter hamburgensis (strain X14 / DSM 10229) GN=lpxB PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 9.594e-11
Identity = 21/70 (30.00%), Postives = 43/70 (61.43%), Query Frame = 1

Query:   97 RVRSEQGMKSLFPMEDIAVMGIWELLPHIYKFRVRLKETIEAALLFRPHAVVTVDSKGFSFRLLKQLRGQ 306
            R  + +G+ SLFP+ED+++MG   ++  +     R++ET +A +   P  +V +DS  F+ R+ +++R +
Sbjct:   49 RSMAREGLVSLFPIEDLSIMGFAAVVKQLPMILRRIRETADAVIAAEPDMLVIIDSPDFTHRVARRVRAR 118

HSP 2 Score: 29.6462 bits (65), Expect = 9.594e-11
Identity = 20/57 (35.09%), Postives = 27/57 (47.37%), Query Frame = 3

Query:  447 ILCILPNE-EAICRLNGLAATFVGHPVVE-------DCLELNLGKGPEPCELKMEGN 593
            +L +LP E E   RL G   T+VGHP++E       D  E      P P  L + G+
Sbjct:  150 VLALLPFEPEEYRRLAGPPCTYVGHPLIEQVGMLRPDAQERQRRDAPPPALLVLPGS 206

HSP 3 Score: 28.8758 bits (63), Expect = 9.594e-11
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  371 YVAPSFWAWKGGEAR 415
            YV+PS WAW+ G AR
Sbjct:  127 YVSPSVWAWRPGRAR 141

The following BLAST results are available for this feature:

BLAST of EY674161 vs. ExPASy Swiss-Prot

BLAST of EY674161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28


Match Name	E-value	Identity	Description
LPXB_RICB8	1.387e-12	35.00	Lipid-A-disaccharide synthase OS=Rickettsia bellii...	[more]
LPXB_DECAR	2.956e-12	32.81	Lipid-A-disaccharide synthase OS=Dechloromonas aro...	[more]
LPXB_BURTA	6.293e-12	37.29	Lipid-A-disaccharide synthase OS=Burkholderia thai...	[more]
LPXB_RICCK	6.297e-12	40.00	Lipid-A-disaccharide synthase OS=Rickettsia canade...	[more]
LPXB_ALCBS	6.300e-12	37.50	Lipid-A-disaccharide synthase OS=Alcanivorax borku...	[more]
LPXB_RICAH	2.216e-11	40.00	Lipid-A-disaccharide synthase OS=Rickettsia akari ...	[more]
LPXB_CHRSD	6.072e-11	37.50	Lipid-A-disaccharide synthase OS=Chromohalobacter ...	[more]
LPXB_NITHX	9.594e-11	30.00	Lipid-A-disaccharide synthase OS=Nitrobacter hambu...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-052-A03-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY674161 ID=EY674161; Name=EY674161; organism=Citrus sinensis; type=EST; length=1029bp
AAAAAAAAAAAAAAAAAAAAAAAAAGAANNTTGGCGCTTGGGGAGCANNN
AAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTCGACCCACGCG
TCCGGTCGGAGCAAGGAATGAAGTCCTTGTTCCCTATGGAAGACATAGCA
GTAATGGGAATTTGGGAGTTGTTACCACATATTTATAAGTTTAGGGTCAG
GTTGAAGGAAACTATAGAAGCTGCTCTTCTGTTTAGGCCTCATGCTGTTG
TGACAGTGGATTCCAAGGGGTTCTCGTTCCGTCTCCTAAAGCAATTACGA
GGACAATCGGGGAATGATTTTTGCAGCTAGATACAGTCACGAAAGATTGA
ATGGACCAGCGCACTTCCATTATGTAGCACCATCATTCTGGGCATGGAAA
GGGGGTGAAGCAAGGCTAAAAAATTTAGCTGCATTTGTGGATCCGCATCT
TGTGCATACTTCCAAATGAGGAAGCCATTTGTAGATTGAATGGACTGGCT
GCAACTTTTGTGGGCCACCCTGTTGTGGAAGATTGTTTGGAGTTGAATTT
GGGGAAGGGACCTGAACCATGTGAATTGAAGATGGAAGGAAACTCTGAAG
AATTTCAAAAATAAATATTCAGTACCTTCAGGAGCCATGATCATTAGCTT
GCTCCCCGGAAGCAGATTACAGGAGGTCGCTCGAATGCTTCCCATCTTTG
CAAAGACTATGGAACTACTAAAAGACTCCTTTTCCCGGAGTTGATAACAG
TCATTCATGTGGGACCTAATCGGGATTGTGAAAATACTTCACCTGGACTT
ATTGGAAATGGCCCGTGCCTTCTATATTGAGTCCTGGAAGATGCTCCAAT
CCAAAATATGATGGATTTCAGGCGAACAGGGGTGGCTTTTTGAACTTCAG
AAACATTGCTTGGGAGCTTCACTTTGCCCAATACCTTGAGGTGTGGGCTT
TCAAACAACTTTCCTACGTGAGGGGTATTCGTATAAAGCAAAAGTCCCAA
ACCTCTCCGCGCAAATTCCCTTATGTCAC