EY649967

Overview
NameEY649967
Unique NameEY649967
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length603
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEPK (Probable M18 family aminopeptidase 2 OS=Pseudomonas putida (strain KT2440) GN=apeB PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.678e-18
Identity = 40/93 (43.01%), Postives = 54/93 (58.06%), Query Frame = 3 
Query:   87 QKVGIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILATSIKEE 365
            Q+ G   +GV+ YGG L   WFDRDL++AGRV  R      G    +L+    P+  IP LAIHL+R  N +G+ +N Q+ L PILA    +E
Sbjct:   97 QRQGFLQLGVEVYGGALLAPWFDRDLSLAGRVTYRR----DGKVESQLIDFKLPVAIIPNLAIHLNRTAN-EGWAINPQNELPPILAQVAGDE 184

HSP 2 Score: 41.5874 bits (96), Expect = 1.678e-18
Identity = 16/29 (55.17%), Postives = 23/29 (79.31%), Query Frame = 2 
Query:   17 NGFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            NG  +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   74 NGIRMVGAHTDSPCLRVKPQPELQRQGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEAE (Probable M18 family aminopeptidase 2 OS=Pseudomonas aeruginosa GN=apeB PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.181e-18
Identity = 38/84 (45.24%), Postives = 52/84 (61.90%), Query Frame = 3 
Query:   96 GIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILA 347
            G   +GV+ YGG L+  WFDRDL++AGRV  R     +G    +LV   + I  IP LAIHL+R  N +G+ +N Q+ L PI+A
Sbjct:  100 GFLQLGVEVYGGALFAPWFDRDLSLAGRVTFR----ANGKLESRLVDFRKAIAVIPNLAIHLNRAAN-EGWPINAQNELPPIIA 178

HSP 2 Score: 43.5134 bits (101), Expect = 2.181e-18
Identity = 16/29 (55.17%), Postives = 24/29 (82.76%), Query Frame = 2 
Query:   17 NGFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            +GF +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   74 SGFRLVGAHTDSPCLRVKPNPEIARNGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEAB (Probable M18 family aminopeptidase 2 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=apeB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.181e-18
Identity = 38/84 (45.24%), Postives = 52/84 (61.90%), Query Frame = 3 
Query:   96 GIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILA 347
            G   +GV+ YGG L+  WFDRDL++AGRV  R     +G    +LV   + I  IP LAIHL+R  N +G+ +N Q+ L PI+A
Sbjct:  100 GFLQLGVEVYGGALFAPWFDRDLSLAGRVTFR----ANGKLESRLVDFRKAIAVIPNLAIHLNRAAN-EGWPINAQNELPPIIA 178

HSP 2 Score: 43.5134 bits (101), Expect = 2.181e-18
Identity = 16/29 (55.17%), Postives = 24/29 (82.76%), Query Frame = 2 
Query:   17 NGFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            +GF +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   74 SGFRLVGAHTDSPCLRVKPNPEIARNGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEA8 (Probable M18 family aminopeptidase 2 OS=Pseudomonas aeruginosa (strain LESB58) GN=apeB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.181e-18
Identity = 38/84 (45.24%), Postives = 52/84 (61.90%), Query Frame = 3 
Query:   96 GIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILA 347
            G   +GV+ YGG L+  WFDRDL++AGRV  R     +G    +LV   + I  IP LAIHL+R  N +G+ +N Q+ L PI+A
Sbjct:  100 GFLQLGVEVYGGALFAPWFDRDLSLAGRVTFR----ANGKLESRLVDFRKAIAVIPNLAIHLNRAAN-EGWPINAQNELPPIIA 178

HSP 2 Score: 43.5134 bits (101), Expect = 2.181e-18
Identity = 16/29 (55.17%), Postives = 24/29 (82.76%), Query Frame = 2 
Query:   17 NGFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            +GF +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   74 SGFRLVGAHTDSPCLRVKPNPEIARNGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEA7 (Probable M18 family aminopeptidase 2 OS=Pseudomonas aeruginosa (strain PA7) GN=apeB PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.181e-18
Identity = 38/84 (45.24%), Postives = 52/84 (61.90%), Query Frame = 3 
Query:   96 GIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILA 347
            G   +GV+ YGG L+  WFDRDL++AGRV  R     +G    +LV   + I  IP LAIHL+R  N +G+ +N Q+ L PI+A
Sbjct:  100 GFLQLGVEVYGGALFAPWFDRDLSLAGRVTFR----ANGKLESRLVDFRKAIAVIPNLAIHLNRAAN-EGWPINAQNELPPIIA 178

HSP 2 Score: 43.5134 bits (101), Expect = 2.181e-18
Identity = 16/29 (55.17%), Postives = 24/29 (82.76%), Query Frame = 2 
Query:   17 NGFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            +GF +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   74 SGFRLVGAHTDSPCLRVKPNPEIARNGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEPW (Probable M18 family aminopeptidase 2 OS=Pseudomonas putida (strain W619) GN=apeB PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.836e-18
Identity = 41/93 (44.09%), Postives = 55/93 (59.14%), Query Frame = 3 
Query:   87 QKVGIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILATSIKEE 365
            Q+ G   +GV+ YGG L   WFDRDL++AGRV  R      G    +L+    PI  IP LAIHL+R  N +G+++N Q+ L PILA    +E
Sbjct:   97 QRQGFLQLGVEVYGGALLAPWFDRDLSLAGRVTFRR----DGKVESQLIDFKLPIAIIPNLAIHLNRTAN-EGWQINPQNELPPILAQVAGDE 184

HSP 2 Score: 39.2762 bits (90), Expect = 2.836e-18
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:   20 GFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            G  +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   75 GIRMVGAHTDSPCLRVKPQPELQRQGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_PSEE4 (Probable M18 family aminopeptidase 2 OS=Pseudomonas entomophila (strain L48) GN=apeB PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.836e-18
Identity = 41/93 (44.09%), Postives = 55/93 (59.14%), Query Frame = 3 
Query:   87 QKVGIWXVGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILATSIKEE 365
            Q+ G   +GV+ YGG L   WFDRDL++AGRV  R      G    +L+    PI  IP LAIHL+R  N +G+++N Q+ L PILA    +E
Sbjct:   97 QRQGFLQLGVEVYGGALLAPWFDRDLSLAGRVTFRR----DGKVESQLIDFKLPIAVIPNLAIHLNRTAN-EGWQINPQTELPPILAQVAGDE 184

HSP 2 Score: 39.2762 bits (90), Expect = 2.836e-18
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:   20 GFHVVGAHTDSPCLKLKPVSKVTKGGYL 103
            G  +VGAHTDSPCL++KP  ++ + G+L
Sbjct:   75 GIRMVGAHTDSPCLRVKPQPELQRQGFL 102
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: DNPEP_CAEEL (Putative aspartyl aminopeptidase OS=Caenorhabditis elegans GN=F01F1.9 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.128e-18
Identity = 49/123 (39.84%), Postives = 70/123 (56.91%), Query Frame = 3 
Query:  108 VGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPILATSIKEELNXVVTDSPNERTD--TYSSKHHSHLLQMNCSQIGCR 470
            VGV TYGGG+W TWFDRDL+VAG VI++ G+       HKL+ + +P++ IP LAIHL+ +  +  FK NT++ L PIL T     +N           D    ++ HH   L +   + GC+
Sbjct:  114 VGVSTYGGGIWRTWFDRDLSVAGLVIVKNGE----KLQHKLIDVKKPVLFIPNLAIHLETDRTT--FKPNTETELRPILETFAAAGINAPQKPESTGFADPRNITNNHHPQFLGLIAKEAGCQ 230
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_CLOAB (Probable M18 family aminopeptidase 2 OS=Clostridium acetobutylicum GN=apeB PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.453e-16
Identity = 39/79 (49.37%), Postives = 54/79 (68.35%), Query Frame = 3 
Query:  108 VGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPIL 344
            +  + YGG +  TWFDR L++AGRV +R GK    P + KL+ I  PI+ IP LAIH++R+VNS GFK+N Q   +PI+
Sbjct:  107 LNTEVYGGPILSTWFDRPLSIAGRVTVR-GKSALFPET-KLLNIKRPILVIPNLAIHMNRDVNS-GFKINPQVDTLPII 182

HSP 2 Score: 34.2686 bits (77), Expect = 1.453e-16
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 2 
Query:    5 YVAGNGFHVVGAHTDSPCLKLKP 73
            +VA  GF ++G HTDSP  ++KP
Sbjct:   72 FVAKKGFKIIGGHTDSPTFRIKP 94
[Close]
BLAST of EY649967 vs. ExPASy Swiss-Prot
Match: APEB_CLONN (Probable M18 family aminopeptidase 2 OS=Clostridium novyi (strain NT) GN=apeB PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 9.618e-15
Identity = 37/79 (46.84%), Postives = 54/79 (68.35%), Query Frame = 3 
Query:  108 VGVQTYGGGLWHTWFDRDLTVAGRVIIREGKDGSGPYSHKLVRIDEPIMRIPTLAIHLDRNVNSDGFKVNTQSHLVPIL 344
            +  + YGG + +TW DR L VAGRV ++ G++   P + KLV I++PIM IP LAIH++RN+N  G ++N Q   +PIL
Sbjct:  107 LNTEVYGGPILNTWIDRPLAVAGRVTLK-GENILFPET-KLVNINKPIMIIPNLAIHMNRNIN-QGIELNRQVDTLPIL 182

HSP 2 Score: 34.2686 bits (77), Expect = 9.618e-15
Identity = 15/34 (44.12%), Postives = 22/34 (64.71%), Query Frame = 2 
Query:    5 YVAGNG------FHVVGAHTDSPCLKLKPVSKVT 88
            +V GNG      F ++GAHTDSP  ++KP  ++T
Sbjct:   66 FVVGNGEVHEDGFKIIGAHTDSPTFRIKPNPEMT 99
The following BLAST results are available for this feature:
BLAST of EY649967 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34
Match NameE-valueIdentityDescription
APEB_PSEPK1.678e-1843.01Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEAE2.181e-1845.24Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEAB2.181e-1845.24Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEA82.181e-1845.24Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEA72.181e-1845.24Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEPW2.836e-1844.09Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
APEB_PSEE42.836e-1844.09Probable M18 family aminopeptidase 2 OS=Pseudomona... [more]
DNPEP_CAEEL3.128e-1839.84Putative aspartyl aminopeptidase OS=Caenorhabditis... [more]
APEB_CLOAB1.453e-1649.37Probable M18 family aminopeptidase 2 OS=Clostridiu... [more]
APEB_CLONN9.618e-1546.84Probable M18 family aminopeptidase 2 OS=Clostridiu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-005-F04-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY649967 ID=EY649967; Name=EY649967; organism=Citrus sinensis; type=EST; length=603bp
AAAATATGTTGCTGGGAATGGATTCCATGTAGTCGGTGCTCATACTGACA
GTCCTTGCCTAAAGCTGAAGCCTGTTTCTAAGGTAACAAAAGGTGGGTAT
TTGGNAGGTTGGCGTCCAAACATATGGAGGTGGTTTGTGGCATACGTGGT
TCGACCGTGACTTGACAGTTGCAGGAAGGGTGATAATTAGAGAAGGGAAA
GATGGTTCTGGTCCCTATTCTCATAAACTAGTTAGAATTGACGAACCCAT
AATGCGAATTCCTACACTGGCAATTCACTTGGACAGGAATGTTAATTCTG
ATGGATTTAAGGTGAACACCCAGAGTCATCTTGTTCCCATCTTGGCAACA
TCAATAAAGGAGGAGCTTAATNAAGTGGTCACAGACAGCCCTAATGAAAG
AACAGACACTTACAGCTCAAAGCATCATTCGCATCTGCTACAGATGAATT
GCAGTCAGATCGGCTGCAGACAGATGATATATNNGTGATTTTGATTTGCA
GCATGTGATACTCAGCCAGTATAGTAGCTGGTATTAAGAAGGAATTNCAT
TTCTCAGGAAGCTCGACATCTCTGCATGTATTCTGCTCTTGAAGGCATNG
ATA
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