EY649900

Overview
NameEY649900
Unique NameEY649900
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length915
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_RABIT (Fructose-bisphosphate aldolase B OS=Oryctolagus cuniculus GN=ALDOB PE=1 SV=3)

HSP 1 Score: 72.7886 bits (177), Expect = 3.127e-12
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  535 KSTQVWCPLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            K  Q   PL     ++  QGLDGLS R   Y + G  F  WR V  I +   S+LA++E    LARYA+I Q N   PIVEP ++ DGDH ++    V ++V A ++
Sbjct:  108 KLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANTLARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVY 214

HSP 2 Score: 45.0542 bits (105), Expect = 7.927e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  438 GAILCDETLYQSTTDGKKMVDVLVEQNIDPGVKVDTGLVPLAGSND*LMVPG 593
            G IL  ETLYQ  + GK   ++L E+ I  G+K+D G  PLAG+N    + G
Sbjct:   76 GVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQG 127

HSP 3 Score: 43.1282 bits (100), Expect = 7.927e-11
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 2 
Query:  257 ELGKTAKTVASPRRGISAMDESNATCGKRLASIGLKNTEANRQAYRTLLVT 409
            EL   A+ + +  +GI A DES  T G RL  I ++NTE NR+ +R +L T
Sbjct:   15 ELSDIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFT 65
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_HUMAN (Fructose-bisphosphate aldolase B OS=Homo sapiens GN=ALDOB PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 4.083e-12
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  535 KSTQVWCPLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            K  Q   PL     ++  QGLDGLS R   Y + G  F  WR V  I +   S+LA++E    LARYA+I Q N   PIVEP ++ DGDH ++    V ++V A ++
Sbjct:  108 KLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVY 214

HSP 2 Score: 45.0542 bits (105), Expect = 7.927e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  438 GAILCDETLYQSTTDGKKMVDVLVEQNIDPGVKVDTGLVPLAGSND*LMVPG 593
            G IL  ETLYQ  + GK   ++L E+ I  G+K+D G  PLAG+N    + G
Sbjct:   76 GVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQG 127

HSP 3 Score: 43.1282 bits (100), Expect = 7.927e-11
Identity = 21/49 (42.86%), Postives = 32/49 (65.31%), Query Frame = 2 
Query:  257 ELGKTAKTVASPRRGISAMDESNATCGKRLASIGLKNTEANRQAYRTLL 403
            EL + A+++ +  +GI A DES  T G RL  I ++NTE NR+ +R +L
Sbjct:   15 ELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREIL 63
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_RAT (Fructose-bisphosphate aldolase B OS=Rattus norvegicus GN=Aldob PE=1 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 6.965e-12
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  535 KSTQVWCPLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            K  Q   PL     ++  QGLDGLS R   Y + G  F  WR V  I +   S+LA++E    LARYA+I Q N   PIVEP ++ DGDH ++    V ++V A ++
Sbjct:  108 KLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRISDQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVLAAVY 214

HSP 2 Score: 45.0542 bits (105), Expect = 7.927e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  438 GAILCDETLYQSTTDGKKMVDVLVEQNIDPGVKVDTGLVPLAGSND*LMVPG 593
            G IL  ETLYQ  + GK   ++L E+ I  G+K+D G  PLAG+N    + G
Sbjct:   76 GVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQG 127

HSP 3 Score: 43.1282 bits (100), Expect = 7.927e-11
Identity = 22/49 (44.90%), Postives = 31/49 (63.27%), Query Frame = 2 
Query:  257 ELGKTAKTVASPRRGISAMDESNATCGKRLASIGLKNTEANRQAYRTLL 403
            EL + A+ + +  +GI A DES  T G RL  I ++NTE NR+ +R LL
Sbjct:   15 ELSEIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFRELL 63
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_PONAB (Fructose-bisphosphate aldolase B OS=Pongo abelii GN=ALDOB PE=2 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 6.965e-12
Identity = 43/107 (40.19%), Postives = 58/107 (54.21%), Query Frame = 1 
Query:  535 KSTQVWCPLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            K  Q   PL     ++  QGLDGLS R   Y + G  F  WR V  I +   S+LA++E    LARYA+I Q N   PIVEP ++ DGDH ++    V ++V A  +
Sbjct:  108 KLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAAY 214

HSP 2 Score: 45.0542 bits (105), Expect = 7.927e-11
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 3 
Query:  438 GAILCDETLYQSTTDGKKMVDVLVEQNIDPGVKVDTGLVPLAGSND*LMVPG 593
            G IL  ETLYQ  + GK   ++L E+ I  G+K+D G  PLAG+N    + G
Sbjct:   76 GVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQG 127

HSP 3 Score: 43.1282 bits (100), Expect = 7.927e-11
Identity = 21/49 (42.86%), Postives = 32/49 (65.31%), Query Frame = 2 
Query:  257 ELGKTAKTVASPRRGISAMDESNATCGKRLASIGLKNTEANRQAYRTLL 403
            EL + A+++ +  +GI A DES  T G RL  I ++NTE NR+ +R +L
Sbjct:   15 ELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREIL 63
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_MOUSE (Fructose-bisphosphate aldolase B OS=Mus musculus GN=Aldob PE=1 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 6.965e-12
Identity = 43/107 (40.19%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  535 KSTQVWCPLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            K  Q   PL     ++  QGLDGLS R   Y + G  F  WR V  I +   S+LA++E    LARYA+I Q N   PIVEP ++ DGDH ++    V ++V A ++
Sbjct:  108 KLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVLAAVY 214
[Close]
BLAST of EY649900 vs. ExPASy Swiss-Prot
Match: ALDOB_CHICK (Fructose-bisphosphate aldolase B OS=Gallus gallus GN=ALDOB PE=3 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 4.515e-11
Identity = 41/100 (41.00%), Postives = 56/100 (56.00%), Query Frame = 1 
Query:  556 PLLVPMTDSWCQGLDGLSSRTGDYYQQGARFA*WRTVWSIPNGI-SALALKEACCDLARYAAIAQDNRSAPIVEP*IVLDGDHGIDRTIAVPKRVRAELF 852
            PL     ++  QGLD L+ R   Y + GA F  WR V  I +   S LA++E    LARYA+I Q N   PIVEP ++ DGDH + R   V ++V A ++
Sbjct:  115 PLAGTNGETTIQGLDKLAERCAQYKKDGADFGKWRAVLKISSTTPSQLAIQENANTLARYASICQQNGLVPIVEPEVLPDGDHDLQRCQYVTEKVLAAVY 214
The following BLAST results are available for this feature:
BLAST of EY649900 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 56
Match NameE-valueIdentityDescription
ALDOB_RABIT3.127e-1240.19Fructose-bisphosphate aldolase B OS=Oryctolagus cu... [more]
ALDOB_HUMAN4.083e-1240.19Fructose-bisphosphate aldolase B OS=Homo sapiens G... [more]
ALDOB_RAT6.965e-1240.19Fructose-bisphosphate aldolase B OS=Rattus norvegi... [more]
ALDOB_PONAB6.965e-1240.19Fructose-bisphosphate aldolase B OS=Pongo abelii G... [more]
ALDOB_MOUSE6.965e-1240.19Fructose-bisphosphate aldolase B OS=Mus musculus G... [more]
ALDOB_CHICK4.515e-1141.00Fructose-bisphosphate aldolase B OS=Gallus gallus ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-004-G09-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY649900 ID=EY649900; Name=EY649900; organism=Citrus sinensis; type=EST; length=915bp
CCACGCGTCCGGAAAGATATTGAGGATAAAATTATAAGTGCTATACGCTA
AGGCCTCTCGAGCTCAGAACCCACAGCAAGAAGAAGCAAAGAGGAAAATG
GCCTCAGCCTCGGCATCTTTACTCAAGTCATCATCCCCCGTTCTTGACAA
GTCTGAGTGGGTTAAGGGCCAGGCCATCCGCCAGTCTACTGGCTCAGTCC
GCAGCCTCCCCAGTGGCCCCTCTTCCCTCACCATCCGTGCTGGTTCCTAT
GCCGATGAGCTTGGCAAGACCGCGAAAACTGTGGCATCACCACGACGTGG
AATTTCGGCCATGGATGAATCAAATGCGACTTGTGGAAAACGCCTTGCCT
CAATTGGGTTAAAGAACACTGAGGCTAACCGCCAGGCATACCGTACCCTC
CTTGTGACAGCCCCTGGTCTTGGACAGTACATCATGTGGTGCCATCCTTT
GTGATGAGACTCTCTACCAATCCACCACCGATGGCAAGAAGATGGTCGAT
GTTCTTGTTGAGCAAAACATTGACCCCGGTGTCAAAGTCGACACAGGTTT
GGTGCCCCTTGCTGGTTCCAATGACTGACTCATGGTGCCAGGGTCTTGAT
GGCCTGTCTTCTCGCACTGGTGATTACTACCAACAGGGAGCTCGTTTCGC
CTAATGGCGTACTGTATGGAGCATTCCCAATGGGATATCTGCCTTGGCAC
TCAAAGAAGCCTGCTGCGATCTTGCCCGCTATGCTGCCATTGCTCAAGAC
AACAGATCGGCTCCAATCGTGGAGCCATAAATCGTGCTCGATGGTGATCA
TGGCATTGACAGGACTATTGCAGTCCCTAAGAGAGTTCGGGCTGAACTCT
TCTTCTACCGAGCTGAGACAATGTCATGCTCGAAGTATCCTCCTCAGCCT
AGCTGGCGCTACTGC
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