EY702100

Overview
NameEY702100
Unique NameEY702100
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length890
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY702100_ssr121 microsatellite EY702100_ssr121:121..144. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_CUCSA (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Cucumis sativus GN=RBCS PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 4.503e-24
Identity = 45/67 (67.16%), Postives = 57/67 (85.07%), Query Frame = 2 
Query:  383 GWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            G+VYRE+H SPG+YDGRYWTMWKLPM+GCTD++QV++E+ E  KEYP +F+R+IGFDN RQV    F
Sbjct:  108 GFVYRENHRSPGFYDGRYWTMWKLPMFGCTDSSQVIQEIEEAKKEYPDAFIRVIGFDNVRQVQCISF 174

HSP 2 Score: 22.3274 bits (46), Expect = 4.503e-24
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  564 KLQCISFLAAKP 599
            ++QCISF+A KP
Sbjct:  168 QVQCISFIAYKP 179
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_HEVBR (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Hevea brasiliensis GN=RBCS PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.508e-24
Identity = 48/70 (68.57%), Postives = 55/70 (78.57%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+ G VYRE+H SPGYYDGRYWTMWKLPM+GCTDA QVL+E+ E+ K YP  + RIIGFDN RQV    F
Sbjct:  105 LEHGTVYREYHRSPGYYDGRYWTMWKLPMFGCTDAVQVLQELDEMIKAYPDCYGRIIGFDNVRQVQCISF 174

HSP 2 Score: 25.0238 bits (53), Expect = 4.508e-24
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  564 KLQCISFLAAKPPG 605
            ++QCISFLA KP G
Sbjct:  168 QVQCISFLAYKPKG 181
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS6_FLAPR (Ribulose bisphosphate carboxylase small chain 6, chloroplastic OS=Flaveria pringlei GN=RBCS6 PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 5.878e-24
Identity = 47/70 (67.14%), Postives = 57/70 (81.43%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+ G+VYRE+  SPGYYDGRYWTMWKLPM+GCTD+ QV+KE+ E  KEYP +++RIIGFDN RQV    F
Sbjct:   96 LEHGFVYRENARSPGYYDGRYWTMWKLPMFGCTDSVQVMKELQECKKEYPQAWIRIIGFDNVRQVQCISF 165

HSP 2 Score: 23.483 bits (49), Expect = 5.878e-24
Identity = 8/12 (66.67%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  564 KLQCISFLAAKP 599
            ++QCISF+A+KP
Sbjct:  159 QVQCISFIASKP 170
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_FLATR (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Flaveria trinervia GN=RBCS PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 7.555e-24
Identity = 49/76 (64.47%), Postives = 60/76 (78.95%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQFPCCQAT 601
            L+ G+VYRE+ SSPGYYDGRYWTMWKLPM+GCTD+ QV+KE+ E  KEYP +++RIIGFDN RQV    F   + T
Sbjct:   96 LEHGFVYRENASSPGYYDGRYWTMWKLPMFGCTDSAQVMKELQECKKEYPQAWIRIIGFDNVRQVQCVSFIASKPT 171
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_FAGCR (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Fagus crenata GN=RBCS1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.635e-24
Identity = 48/70 (68.57%), Postives = 55/70 (78.57%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+  +VYRE++ SPGYYDGRYW MWKLPM+GCTDATQVL E+ E  K YP S +RIIGFDNKRQV    F
Sbjct:  105 LEHPFVYRENNRSPGYYDGRYWVMWKLPMFGCTDATQVLAELQEASKTYPTSHIRIIGFDNKRQVQCISF 174

HSP 2 Score: 25.0238 bits (53), Expect = 7.635e-24
Identity = 9/13 (69.23%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  564 KLQCISFLAAKPP 602
            ++QCISF+A KPP
Sbjct:  168 QVQCISFIAYKPP 180
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_STELP (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Stellaria longipes GN=RBCS PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 7.640e-24
Identity = 49/69 (71.01%), Postives = 54/69 (78.26%), Query Frame = 2 
Query:  377 QKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            + G+VYREH SSPGYYDGRYWTMWKLPM+GCTDATQV  EV E  K YP + +RIIGFDN RQV    F
Sbjct:  104 EHGFVYREHTSSPGYYDGRYWTMWKLPMFGCTDATQVAAEVQEAKKAYPDAHIRIIGFDNVRQVQCISF 172

HSP 2 Score: 22.3274 bits (46), Expect = 7.640e-24
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  564 KLQCISFLAAKP 599
            ++QCISF+A KP
Sbjct:  166 QVQCISFIAYKP 177
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_HELAN (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Helianthus annuus GN=RBCS PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 7.645e-24
Identity = 47/70 (67.14%), Postives = 57/70 (81.43%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+ G+VYRE+  SPGYYDGRYWTMWKLPM+GCTD+ QV+KE+ E  KEYP +++RIIGFDN RQV    F
Sbjct:  101 LEHGFVYRENARSPGYYDGRYWTMWKLPMFGCTDSAQVMKELAECKKEYPQAWIRIIGFDNVRQVQCIMF 170

HSP 2 Score: 22.3274 bits (46), Expect = 7.645e-24
Identity = 7/14 (50.00%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  564 KLQCISFLAAKPPG 605
            ++QCI F+A++P G
Sbjct:  164 QVQCIMFIASRPDG 177
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_MEDSA (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.289e-23
Identity = 47/70 (67.14%), Postives = 58/70 (82.86%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+KG+VYRE+H SPGYYDGRYWTMW+LP++G TD++QVLKE+ +   EYP SF+RIIGFDN RQV    F
Sbjct:  103 LEKGFVYRENHRSPGYYDGRYWTMWRLPLFGATDSSQVLKELADCKAEYPDSFIRIIGFDNVRQVQCISF 172
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS2_AMAHP (Ribulose bisphosphate carboxylase small chain 2, chloroplastic OS=Amaranthus hypochondriacus GN=RBCS2 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.293e-23
Identity = 49/70 (70.00%), Postives = 54/70 (77.14%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+  +VYRE+H SPGY DGRYWTMWKLPMYGCTD  QVL EV E  K YP +F+RIIGFDNKRQV    F
Sbjct:  106 LEHPFVYRENHRSPGYQDGRYWTMWKLPMYGCTDPAQVLNEVEEAKKAYPTAFIRIIGFDNKRQVQCVSF 175

HSP 2 Score: 21.9422 bits (45), Expect = 1.293e-23
Identity = 7/12 (58.33%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  564 KLQCISFLAAKP 599
            ++QC+SF+A KP
Sbjct:  169 QVQCVSFIAYKP 180
[Close]
BLAST of EY702100 vs. ExPASy Swiss-Prot
Match: RBS_TRIRP (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Trifolium repens GN=RBCS PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.853e-23
Identity = 47/70 (67.14%), Postives = 58/70 (82.86%), Query Frame = 2 
Query:  374 LQKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVHKEYPHSFVRIIGFDNKRQVAVHQF 583
            L+KG+V+R+++SSPGYYDGRYWTMW+LP++G TDA QVLKEV E   EYP +F+RIIGFDN RQV    F
Sbjct:  101 LEKGFVHRQYNSSPGYYDGRYWTMWRLPLFGTTDAAQVLKEVAECKAEYPEAFIRIIGFDNVRQVQCISF 170

HSP 2 Score: 21.1718 bits (43), Expect = 2.853e-23
Identity = 7/12 (58.33%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  564 KLQCISFLAAKP 599
            ++QCISF+A+ P
Sbjct:  164 QVQCISFIASTP 175
The following BLAST results are available for this feature:
BLAST of EY702100 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 108
Match NameE-valueIdentityDescription
RBS_CUCSA4.503e-2467.16Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_HEVBR4.508e-2468.57Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS6_FLAPR5.878e-2467.14Ribulose bisphosphate carboxylase small chain 6, c... [more]
RBS_FLATR7.555e-2464.47Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_FAGCR7.635e-2468.57Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_STELP7.640e-2471.01Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_HELAN7.645e-2467.14Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS_MEDSA1.289e-2367.14Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS2_AMAHP1.293e-2370.00Ribulose bisphosphate carboxylase small chain 2, c... [more]
RBS_TRIRP2.853e-2367.14Ribulose bisphosphate carboxylase small chain, chl... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-001-A12-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702100 ID=EY702100; Name=EY702100; organism=Citrus sinensis; type=EST; length=890bp
GGGGGGGGGGGGGGGGGGGGNTTTTTTTTATNTAATGGTNNNNNNNNNNN
NNNNNNNNNNNNNNNTTCGTCGACCCACGCGTCCGGGATTCCCTGCTCGG
AATTCGAGTTGGAGGTCATTTCTCTCTCTCTCTCTCTCTCTCTCTGTCTC
TCTTAATATATACTATTATTTTGACGGAACCTTATCTATGTGCTTTAGGA
GAAAAATTACACATGTCATGAATCTATTGGTCAGAGATATCCATTCAATA
TCAAAACCTGCATTAACAATTGCTTACTTAAAATCTAAGCTTTTGGGTTA
AGCGGATTTTATCAGGCAAATGGGTGGTGATATTTGATTTTTTTTTTTTT
GTTTTTTCTTTTGGGTGGCGTGACTGCAGAAAGGATGGGTGTACCGTGAG
CACCACAGCTCACCAGGATACTACGATGGACGCTACTGGACAATGTGGAA
GCTGCCCATGTATGGGTGCACAGATGCAACCCAGGTTTTGAAGGAGGTTG
GGGAGGTCCATAAGGAATACCCACATTCATTCGTCCGAATTATCGGATTT
GACAACAAGCGTCAAGTTGCAGTGCATCAGTTTCCTTGCTGCCAAGCCAC
CAGGCGTCTAGGCTCCCCATTAAATCTGCGTAACCACCCATGCCATATTG
TTGATTATAATTAAGCGGAACCCCCATAATGATCATTCGATCAATGGGGG
TTCCGTTTAGCGTCAATTTGCACTTAAGGCTGCTGTCGTGGCTGGTCAAA
TTTTTTTGGTTTTCTCCCCGAACTGGGCCTTTTTTTCTTTTAAAAACATT
TTAATAAATGGAATCGCTAAAAAAGAAGACAAAAAAGAAACTATTTCAAA
TGAACATTTCCTTCCTTTATCTTTTACATCTAATCCAGCT
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