DR908229 | Citrus Genome Database

Overview


Name	DR908229
Unique Name	DR908229
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	689

Libraries


Library Name	Type
Citrus sinensis phloem	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAS_LACBI (Ras-like protein OS=Laccaria bicolor PE=2 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 5.940e-12
Identity = 25/88 (28.41%), Postives = 48/88 (54.55%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFL 265
            GK+ L  Q +   F ++Y  TI   +  K+   +D +  L + DTAGQE + ++   + R  +  +LVY + S +SF+ +  ++++ L
Sbjct:   20 GKSCLTIQLIQSHFVDEYDPTIEESY-RKQCVIDDEVALLDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEIMTFQQQIL 106

HSP 2 Score: 40.817 bits (94), Expect = 5.940e-12
Identity = 24/64 (37.50%), Postives = 36/64 (56.25%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEE 489
            P +V+GNK D+D    RVVS+++  +     G   + ETSAK  INVE AF  + +   +  +E
Sbjct:  115 PIIVVGNKCDLD--KERVVSKQEGESLARQFG-CKFIETSAKSRINVENAFYDLVREIRRYNKE 175

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAS1_HYDMA (Ras-like protein RAS1 OS=Hydra magnipapillata GN=RAS1 PE=2 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 5.962e-12
Identity = 30/102 (29.41%), Postives = 53/102 (51.96%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDGAPLL 307
            GK++L  Q +   F   Y  TI   ++ K+V  +  +  L I DTAGQE + ++   + R  +  + ++ V S+KSF  ++N+R + L      D D  P++
Sbjct:   21 GKSALTIQLIQNHFVEDYDPTIEDSYI-KQVVVDGAICILDILDTAGQEEYSAMREHYMRTGEGFLCIFAVTSLKSFQEIDNFRTQAL---RVKDADKVPMV 118

HSP 2 Score: 40.0466 bits (92), Expect = 5.962e-12
Identity = 27/66 (40.91%), Postives = 34/66 (51.52%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEE 495
            P V++GNK+D+     R VS K       S G IPY ETSAK    VEEAF  + +  L     +E
Sbjct:  116 PMVLVGNKVDLP---KRDVSTKDGNDKALSFG-IPYVETSAKTKQGVEEAFFTLVREILADRRNQE 177

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RASK_HUMAN (GTPase KRas OS=Homo sapiens GN=KRAS PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 5.971e-12
Identity = 27/86 (31.40%), Postives = 50/86 (58.14%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GK++L  Q +   F ++Y  TI  D   K+V  +     L I DTAGQE + ++   + R  +  + V+ +N+ KSF++++++RE+
Sbjct:   15 GKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQ 99

HSP 2 Score: 38.891 bits (89), Expect = 5.971e-12
Identity = 24/56 (42.86%), Postives = 33/56 (58.93%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAK 465
            P V++GNK D+    SR V  K+A+    S G IP+ ETSAK    VE+AF  + +
Sbjct:  110 PMVLVGNKCDLP---SRTVDTKQAQDLARSYG-IPFIETSAKTRQRVEDAFYTLVR 161

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RASK_CYPCA (GTPase KRas OS=Cyprinus carpio GN=kras PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 5.971e-12
Identity = 27/86 (31.40%), Postives = 50/86 (58.14%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GK++L  Q +   F ++Y  TI  D   K+V  +     L I DTAGQE + ++   + R  +  + V+ +N+ KSF++++++RE+
Sbjct:   15 GKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQ 99

HSP 2 Score: 38.891 bits (89), Expect = 5.971e-12
Identity = 25/64 (39.06%), Postives = 36/64 (56.25%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEE 489
            P V++GNK D+    SR V  K+A+    S G IP+ ETSAK    V++AF  + +   K  E+
Sbjct:  110 PMVLVGNKCDLP---SRSVDTKQAQDLARSYG-IPFIETSAKTRQGVDDAFYTLVREIRKHKEK 169

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RASH_MOUSE (GTPase HRas OS=Mus musculus GN=Hras1 PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 5.971e-12
Identity = 29/102 (28.43%), Postives = 53/102 (51.96%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDGAPLL 307
            GK++L  Q +   F ++Y  TI  D   K+V  +     L   DTAGQE + ++   + R  +  + V+ +N+ KSF++++ +RE+        D D  P++
Sbjct:   15 GKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDYLDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQI---KRVKDSDDVPMV 112

HSP 2 Score: 40.4318 bits (93), Expect = 5.971e-12
Identity = 29/88 (32.95%), Postives = 45/88 (51.14%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSSQPRSSGCEC 561
            P V++GNK D+    +R V  ++A+    S G IPY ETSAK    VE+AF  + +  ++  +  ++  PD       S P    C+C
Sbjct:  110 PMVLVGNKCDLA---ARTVESRQAQDLARSYG-IPYIETSAKTRQGVEDAFYTLVRE-IRQHKLRKLNPPD------ESGPGCMSCKC 186

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RB33B_MOUSE (Ras-related protein Rab-33B OS=Mus musculus GN=Rab33b PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.385e-12
Identity = 36/87 (41.38%), Postives = 52/87 (59.77%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERF-QSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GKT L  ++   +F ++ +ATIG DF  + V  +     +Q+WDTAGQERF +S+   +YR     V VYD+ +M SF +L  W EE
Sbjct:   45 GKTCLTYRFCAGRFPDRTEATIGVDFRERAVDIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPAWIEE 131

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSJ (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. japonica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 7.694e-12
Identity = 24/86 (27.91%), Postives = 44/86 (51.16%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GKT+ + +++  +F  +Y+ TIG +    +            WDTAGQE+F  L   +Y    C ++++DV S  ++ N+  W  +
Sbjct:   25 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRD 110

HSP 2 Score: 34.2686 bits (77), Expect = 7.694e-12
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAK 465
            P V+ GNK+DV    +R V  K+       K N+ Y+E SAK   N E+ F  +A+
Sbjct:  119 PIVLCGNKVDV---KNRQVKAKQVTFH--RKKNLQYYEISAKSNYNFEKPFLYLAR 169

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSI (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. indica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 7.694e-12
Identity = 24/86 (27.91%), Postives = 44/86 (51.16%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GKT+ + +++  +F  +Y+ TIG +    +            WDTAGQE+F  L   +Y    C ++++DV S  ++ N+  W  +
Sbjct:   25 GKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRD 110

HSP 2 Score: 34.2686 bits (77), Expect = 7.694e-12
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAK 465
            P V+ GNK+DV    +R V  K+       K N+ Y+E SAK   N E+ F  +A+
Sbjct:  119 PIVLCGNKVDV---KNRQVKAKQVTFH--RKKNLQYYEISAKSNYNFEKPFLYLAR 169

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAS_BOTFU (Ras-like protein OS=Botryotinia fuckeliana GN=ras1 PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 7.708e-12
Identity = 25/88 (28.41%), Postives = 48/88 (54.55%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFL 265
            GK+ L  Q +   F ++Y  TI  D   K+   ++ +  L + DTAGQE + ++   + R  +  +LVY + S +SF+ +  ++++ L
Sbjct:   20 GKSCLTIQLIQSHFVDEYDPTI-EDSYRKQCVIDEEVALLDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEIMTFQQQIL 106

HSP 2 Score: 41.2022 bits (95), Expect = 7.708e-12
Identity = 30/98 (30.61%), Postives = 45/98 (45.92%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSSQP----------RSSGCEC 561
            P +V+GNK D++G   R VS+++ +      G   + ETSAK  INV+ AF  I +   +  +E   Y        N+  P          + SGC C
Sbjct:  115 PIIVVGNKCDLEG--ERQVSKQEGQQLADDFG-CKFIETSAKSRINVDNAFYDIVREIRRYNKEMAGYTAGGASGANNGGPQGKMEVSEGEKESGCCC 209

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RASK_RIVMA (GTPase KRas OS=Rivulus marmoratus GN=kras PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.754e-12
Identity = 27/86 (31.40%), Postives = 50/86 (58.14%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GK++L  Q +   F ++Y  TI  D   K+V  +     L I DTAGQE + ++   + R  +  + V+ +N+ KSF++++++RE+
Sbjct:   15 GKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQ 99

HSP 2 Score: 38.5058 bits (88), Expect = 7.754e-12
Identity = 24/64 (37.50%), Postives = 36/64 (56.25%), Query Frame = 1

Query:  298 PFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEE 489
            P V++GNK D+    +R V  K+A+    S G IP+ ETSAK    V++AF  + +   K  E+
Sbjct:  110 PMVLVGNKYDLP---TRTVDTKQAQDLARSYG-IPFIETSAKTRQGVDDAFYTLVREIRKHKEK 169

The following BLAST results are available for this feature:

BLAST of DR908229 vs. ExPASy Swiss-Prot

BLAST of DR908229 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
RAS_LACBI	5.940e-12	28.41	Ras-like protein OS=Laccaria bicolor PE=2 SV=1	[more]
RAS1_HYDMA	5.962e-12	29.41	Ras-like protein RAS1 OS=Hydra magnipapillata GN=R...	[more]
RASK_HUMAN	5.971e-12	31.40	GTPase KRas OS=Homo sapiens GN=KRAS PE=1 SV=1	[more]
RASK_CYPCA	5.971e-12	31.40	GTPase KRas OS=Cyprinus carpio GN=kras PE=2 SV=1	[more]
RASH_MOUSE	5.971e-12	28.43	GTPase HRas OS=Mus musculus GN=Hras1 PE=1 SV=1	[more]
RB33B_MOUSE	7.385e-12	41.38	Ras-related protein Rab-33B OS=Mus musculus GN=Rab...	[more]
RAN2_ORYSJ	7.694e-12	27.91	GTP-binding nuclear protein Ran-2 OS=Oryza sativa ...	[more]
RAN2_ORYSI	7.694e-12	27.91	GTP-binding nuclear protein Ran-2 OS=Oryza sativa ...	[more]
RAS_BOTFU	7.708e-12	28.41	Ras-like protein OS=Botryotinia fuckeliana GN=ras1...	[more]
RASK_RIVMA	7.754e-12	31.40	GTPase KRas OS=Rivulus marmoratus GN=kras PE=2 SV=...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_16357 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-34_C05 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR908229 ID=DR908229; Name=DR908229; organism=Citrus sinensis; type=EST; length=689bp
GGGCAAGACCTCTTTGATGAATCAATATGTAAACAAGAAGTTTAGCAATC
AGTACAAGGCGACCATCGGAGCTGATTTCTTGACAAAGGAAGTGCAGTTT
GAAGATAGGCTCTTCACCTTACAGATCTGGGATACAGCTGGTCAGGAAAG
ATTCCAAAGCCTGGGTGTTGCTTTCTACCGAGGTGCAGATTGCTGTGTAC
TTGTGTATGATGTAAATTCTATGAAGTCCTTTGACAACCTCAATAACTGG
AGAGAAGAATTCCTCATTCAGGCTAGCCCTTCGGATCCAGACGGGGCCCC
TTTGTTGTTTTAGGAAATAAGATTGATGTAGATGGTGGAAACAGTAGGGT
GGTTTCGGAGAAGAAGGCTCGAGCATGGTGTGCTTCCAAAGGAAATATTC
CTTACTTTGAGACCTCTGCAAAAGAAGGCATTAATGTGGAAGAAGCTTTC
CAGTGCATAGCTAAAAATGCTTTGAAGAGTGGGGAGGAGGAAGAGATATA
CTTGCCGGACACCATTGATGTTGGTAACAGCAGCCAGCCAAGGTCAAGTG
GATGCGAGTGCTAAAGATATGGTCTAATTTTTTCAGGAGCAATAAGCAGT
ATAACAAATGATTTGGCCTTTTTAACCATTTTTATGGGTATGTATAAGGT
TACATTGTACATTAGTGTTTGTGTGAAACAAATTGGTTC