DR908229 | Citrus Genome Database

Overview


Name	DR908229
Unique Name	DR908229
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	689

Libraries


Library Name	Type
Citrus sinensis phloem	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB5C_MOUSE (Ras-related protein Rab-5C OS=Mus musculus GN=Rab5c PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 4.289e-22
Identity = 41/94 (43.62%), Postives = 60/94 (63.83%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPS 283
            GK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ +  +F    NW +E   QASP+
Sbjct:   33 GKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPN 126

HSP 2 Score: 37.7354 bits (86), Expect = 4.289e-22
Identity = 27/84 (32.14%), Postives = 42/84 (50.00%), Query Frame = 1

Query:  313 GNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDT---IDVGNSSQPRSSGC 555
            GNK D+   + R V  ++A+A+ A   ++ + ETSAK  +NV E F  IAK   K+  +     P     +D+  S+    S C
Sbjct:  133 GNKADL--ASKRAVEFQEAQAY-ADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNAAGAPGRTRGVDLQESNPASRSQC 213

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8A_MACFA (Ras-related protein Rab-8A OS=Macaca fascicularis GN=RAB8A PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.294e-22
Identity = 37/83 (44.58%), Postives = 58/83 (69.88%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNW 102

HSP 2 Score: 39.2762 bits (90), Expect = 4.294e-22
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSSQPRSSG 552
            ++LGNK DV+  + R VS+++        G I + ETSAK  INVE AF  +A++ +K+  ++++        GNS Q  + G
Sbjct:  117 MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAKANINVENAFFTLARD-IKAKMDKKL-------EGNSPQGSNQG 188

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8A_HUMAN (Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8A PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.294e-22
Identity = 37/83 (44.58%), Postives = 58/83 (69.88%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNW 102

HSP 2 Score: 39.2762 bits (90), Expect = 4.294e-22
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSSQPRSSG 552
            ++LGNK DV+  + R VS+++        G I + ETSAK  INVE AF  +A++ +K+  ++++        GNS Q  + G
Sbjct:  117 MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAKANINVENAFFTLARD-IKAKMDKKL-------EGNSPQGSNQG 188

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8A_CANFA (Ras-related protein Rab-8A OS=Canis familiaris GN=RAB8A PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.294e-22
Identity = 37/83 (44.58%), Postives = 58/83 (69.88%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNW 102

HSP 2 Score: 39.2762 bits (90), Expect = 4.294e-22
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSSQPRSSG 552
            ++LGNK DV+  + R VS+++        G I + ETSAK  INVE AF  +A++ +K+  ++++        GNS Q  + G
Sbjct:  117 MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAKANINVENAFFTLARD-IKAKMDKKL-------EGNSPQGSNQG 188

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8A_DICDI (Ras-related protein Rab-8A OS=Dictyostelium discoideum GN=rab8A PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.294e-22
Identity = 38/83 (45.78%), Postives = 54/83 (65.06%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GK+ L+ ++    F+  +  TIG DF  + ++ E +   LQIWDTAGQERF+++  A+YRGA   +LVYDV   KSF N+ NW
Sbjct:   27 GKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELEGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEKSFGNIRNW 109

HSP 2 Score: 41.9726 bits (97), Expect = 4.294e-22
Identity = 28/89 (31.46%), Postives = 49/89 (55.06%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALK-----SGEEEEIYLPDTIDVGNSSQPRSSGC 555
            +++GNK D+     +VV   + ++  A +  I + ETSAK  INVEEAF  +AK+  K       E+ ++  P T ++G ++  + + C
Sbjct:  124 MLIGNKCDM--AEKKVVDSSRGKS-LADEYGIKFLETSAKNSINVEEAFISLAKDIKKRMIDTPNEQPQVVQPGT-NLGANNNKKKACC 208

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB10_CANFA (Ras-related protein Rab-10 OS=Canis familiaris GN=RAB10 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 4.298e-22
Identity = 36/83 (43.37%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT ++ ++ +  F+  + +TIG DF  K V+ + +   LQIWDTAGQERF ++  ++YRGA   +LVYD+ + KSF+N++ W
Sbjct:   21 GKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKW 103

HSP 2 Score: 43.1282 bits (100), Expect = 4.298e-22
Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGNSS 534
            ++LGNK D+D  + RVV + K        G I +FETSAK  IN+E+AF  +A++ L+    +E   P++ +V  SS
Sbjct:  118 MLLGNKCDMD--DKRVVPKGKGEQIAREHG-IRFFETSAKVNINIEKAFLTLAEDILRKTPVKE---PNSENVDISS 188

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RIC1_ORYSJ (Ras-related protein RIC1 OS=Oryza sativa subsp. japonica GN=RIC1 PE=2 SV=2)

HSP 1 Score: 80.8777 bits (198), Expect = 4.298e-22
Identity = 35/86 (40.70%), Postives = 56/86 (65.12%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREE 259
            GK+ L+ ++ +  +   Y +TIG DF  + V+ + +   LQIWDTAGQERF+++  ++YRGA   ++VYDV   +SF+N+  W  E
Sbjct:   20 GKSCLLLRFADDSYLESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNE 105

HSP 2 Score: 44.669 bits (104), Expect = 4.298e-22
Identity = 30/87 (34.48%), Postives = 46/87 (52.87%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIA---KNALKSGEEEEIYLPDTIDVGNSSQPRSSGC 555
            +++GNK D+    +RVVS +  +A  A +  IP+ ETSAK+  NVE+AF  +A   KN + S        P T+ +      + S C
Sbjct:  117 LLVGNKCDL--AENRVVSYEAGKA-LADEIGIPFLETSAKDATNVEKAFMTMAGEIKNRMASQPATNASKPATVQMRGQPVAQQSSC 200

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8B_RAT (Ras-related protein Rab-8B OS=Rattus norvegicus GN=Rab8b PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.587e-22
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT L+ ++    F+  + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNW 102

HSP 2 Score: 38.1206 bits (87), Expect = 5.587e-22
Identity = 22/57 (38.60%), Postives = 35/57 (61.40%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNAL 474
            ++LGNK D++  + R VS+++        G I + ETSAK   NVEEAF  +A++ +
Sbjct:  117 MILGNKCDMN--DKRQVSKERGEKLAIDYG-IKFLETSAKSSTNVEEAFFTLARDIM 170

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8B_PONAB (Ras-related protein Rab-8B OS=Pongo abelii GN=RAB8B PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.587e-22
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT L+ ++    F+  + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNW 102

HSP 2 Score: 38.1206 bits (87), Expect = 5.587e-22
Identity = 22/57 (38.60%), Postives = 35/57 (61.40%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNAL 474
            ++LGNK D++  + R VS+++        G I + ETSAK   NVEEAF  +A++ +
Sbjct:  117 MILGNKCDMN--DKRQVSKERGEKLAIDYG-IKFLETSAKSSTNVEEAFFTLARDIM 170

[Close]

BLAST of DR908229 vs. ExPASy Swiss-Prot
Match: RAB8B_MOUSE (Ras-related protein Rab-8B OS=Mus musculus GN=Rab8b PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.587e-22
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 GKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNW 250
            GKT L+ ++    F+  + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW
Sbjct:   20 GKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNW 102

HSP 2 Score: 38.1206 bits (87), Expect = 5.587e-22
Identity = 22/57 (38.60%), Postives = 35/57 (61.40%), Query Frame = 1

Query:  304 VVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFQCIAKNAL 474
            ++LGNK D++  + R VS+++        G I + ETSAK   NVEEAF  +A++ +
Sbjct:  117 MILGNKCDMN--DKRQVSKERGEKLAIDYG-IKFLETSAKSSTNVEEAFFTLARDIM 170

The following BLAST results are available for this feature:

BLAST of DR908229 vs. ExPASy Swiss-Prot

BLAST of DR908229 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
RAB5C_MOUSE	4.289e-22	43.62	Ras-related protein Rab-5C OS=Mus musculus GN=Rab5...	[more]
RAB8A_MACFA	4.294e-22	44.58	Ras-related protein Rab-8A OS=Macaca fascicularis ...	[more]
RAB8A_HUMAN	4.294e-22	44.58	Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8...	[more]
RAB8A_CANFA	4.294e-22	44.58	Ras-related protein Rab-8A OS=Canis familiaris GN=...	[more]
RAB8A_DICDI	4.294e-22	45.78	Ras-related protein Rab-8A OS=Dictyostelium discoi...	[more]
RAB10_CANFA	4.298e-22	43.37	Ras-related protein Rab-10 OS=Canis familiaris GN=...	[more]
RIC1_ORYSJ	4.298e-22	40.70	Ras-related protein RIC1 OS=Oryza sativa subsp. ja...	[more]
RAB8B_RAT	5.587e-22	45.78	Ras-related protein Rab-8B OS=Rattus norvegicus GN...	[more]
RAB8B_PONAB	5.587e-22	45.78	Ras-related protein Rab-8B OS=Pongo abelii GN=RAB8...	[more]
RAB8B_MOUSE	5.587e-22	45.78	Ras-related protein Rab-8B OS=Mus musculus GN=Rab8...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_16357 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-34_C05 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR908229 ID=DR908229; Name=DR908229; organism=Citrus sinensis; type=EST; length=689bp
GGGCAAGACCTCTTTGATGAATCAATATGTAAACAAGAAGTTTAGCAATC
AGTACAAGGCGACCATCGGAGCTGATTTCTTGACAAAGGAAGTGCAGTTT
GAAGATAGGCTCTTCACCTTACAGATCTGGGATACAGCTGGTCAGGAAAG
ATTCCAAAGCCTGGGTGTTGCTTTCTACCGAGGTGCAGATTGCTGTGTAC
TTGTGTATGATGTAAATTCTATGAAGTCCTTTGACAACCTCAATAACTGG
AGAGAAGAATTCCTCATTCAGGCTAGCCCTTCGGATCCAGACGGGGCCCC
TTTGTTGTTTTAGGAAATAAGATTGATGTAGATGGTGGAAACAGTAGGGT
GGTTTCGGAGAAGAAGGCTCGAGCATGGTGTGCTTCCAAAGGAAATATTC
CTTACTTTGAGACCTCTGCAAAAGAAGGCATTAATGTGGAAGAAGCTTTC
CAGTGCATAGCTAAAAATGCTTTGAAGAGTGGGGAGGAGGAAGAGATATA
CTTGCCGGACACCATTGATGTTGGTAACAGCAGCCAGCCAAGGTCAAGTG
GATGCGAGTGCTAAAGATATGGTCTAATTTTTTCAGGAGCAATAAGCAGT
ATAACAAATGATTTGGCCTTTTTAACCATTTTTATGGGTATGTATAAGGT
TACATTGTACATTAGTGTTTGTGTGAAACAAATTGGTTC