DR406179 | Citrus Genome Database

Overview


Name	DR406179
Unique Name	DR406179
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	745

Libraries


Library Name	Type
Mature Juice Sac from Navel Orange in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
DR406179_ssr44	microsatellite	DR406179_ssr44:44..58.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_ASPFC (ATPase get3 OS=Aspergillus fumigatus (strain CEA10 / CBS 144.89 / FGSC A1163) GN=get3 PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 1.046e-47
Identity = 92/186 (49.46%), Postives = 127/186 (68.28%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEEETGSTE----------GMDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            ++ E ++++IL Q +L+W+F            S  L+I LA+VR SVL+ISTDPAHNLSDAF Q+F K   LV+G+SNL AME+DP  S+++   S +          GM ++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   11 DLMEPTLQSILSQKTLRWIFVGGKGGVGKTTTSCSLAIQLAKVRKSVLLISTDPAHNLSDAFGQKFGKEARLVDGYSNLSAMEIDPNGSIQDLLASGDSQGDDPLAGLGMGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 196

HSP 2 Score: 36.965 bits (84), Expect = 1.046e-47
Identity = 15/30 (50.00%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFK+PD+TTFV
Sbjct:  210 DELLQKMESLRETISEVNTQFKNPDMTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_ASPOR (ATPase get3 OS=Aspergillus oryzae GN=get3 PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.363e-47
Identity = 91/186 (48.92%), Postives = 127/186 (68.28%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEEETGSTEG----------MDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            ++ E S+++I+ QD+L+W+F            S  L+I LA+ R SVL+ISTDPAHNLSDAF Q+F K   LV+G++NL AME+DP  S+++   S EG          + ++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   10 DLMEPSLQSIVSQDTLRWIFVGGKGGVGKTTTSCSLAIQLAKARKSVLLISTDPAHNLSDAFGQKFGKEARLVDGYTNLSAMEIDPNGSIQDLLASGEGQGDDPMAGLGVGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 195

HSP 2 Score: 36.965 bits (84), Expect = 1.363e-47
Identity = 15/30 (50.00%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFK+PD+TTFV
Sbjct:  209 DELLQKMESLRETISEVNTQFKNPDMTTFV 238

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_ASPFN (ATPase get3 OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) GN=get3 PE=3 SV=2)

HSP 1 Score: 172.555 bits (436), Expect = 1.363e-47
Identity = 91/186 (48.92%), Postives = 127/186 (68.28%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEEETGSTEG----------MDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            ++ E S+++I+ QD+L+W+F            S  L+I LA+ R SVL+ISTDPAHNLSDAF Q+F K   LV+G++NL AME+DP  S+++   S EG          + ++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   10 DLMEPSLQSIVSQDTLRWIFVGGKGGVGKTTTSCSLAIQLAKARKSVLLISTDPAHNLSDAFGQKFGKEARLVDGYTNLSAMEIDPNGSIQDLLASGEGQGDDPMAGLGVGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 195

HSP 2 Score: 36.965 bits (84), Expect = 1.363e-47
Identity = 15/30 (50.00%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFK+PD+TTFV
Sbjct:  209 DELLQKMESLRETISEVNTQFKNPDMTTFV 238

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_ASPTN (ATPase get3 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=get3 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.776e-47
Identity = 91/186 (48.92%), Postives = 128/186 (68.82%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEEETGSTE----------GMDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            ++ E S+++I+ Q +L+W+F            S  L+I LA+VR SVL+ISTDPAHNLSDAF Q+F K   LV+GF+NL AME+DP  S+++   S +          G++++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   11 DLMEPSLQSIVNQKTLRWIFVGGKGGVGKTTTSCSLAIQLAKVRKSVLLISTDPAHNLSDAFGQKFGKEARLVDGFTNLSAMEIDPNGSIQDLLASGDAQQDDPMAGLGVNNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 196

HSP 2 Score: 36.965 bits (84), Expect = 1.776e-47
Identity = 16/30 (53.33%), Postives = 21/30 (70.00%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFKD DLTTFV
Sbjct:  210 DELLQKMESLRETISEVNSQFKDADLTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_PENMQ (ATPase get3 OS=Penicillium marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) GN=get3 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.315e-47
Identity = 91/186 (48.92%), Postives = 125/186 (67.20%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDPS------------VEEETGSTEGMDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            E  E ++++IL+Q +L+W+F            S  L+I L++VR SVL+ISTDPAHNLSDAF Q+F K   LV+GF+NL AME+DP+             +E+  +  GM  +  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   11 EALEPTLQSILDQKTLRWIFVGGKGGVGKTTTSCSLAIQLSKVRKSVLLISTDPAHNLSDAFGQKFGKEARLVDGFTNLSAMEIDPNGSIQDLLASGGEAQEDPLAGLGMGGMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 196

HSP 2 Score: 36.5798 bits (83), Expect = 2.315e-47
Identity = 14/30 (46.67%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D L+ ++  +++ I  VN QFK+PD+TTFV
Sbjct:  210 DDLMSKMESLRETISEVNTQFKNPDMTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_ASPCL (ATPase get3 OS=Aspergillus clavatus GN=get3 PE=3 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 2.315e-47
Identity = 91/186 (48.92%), Postives = 126/186 (67.74%), Query Frame = 2

Query:   95 EIPEGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEEETGSTE----------GMDSLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            ++ E ++++I+ Q +L+W+F            S  L+I LA+ R SVL+ISTDPAHNLSDAF Q+F K   LV+G+SNL AME+DP  S+++   S E          GM ++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L ++FG M+N +
Sbjct:   11 DLMEPTLQSIINQKTLRWIFVGGKGGVGKTTTSCSLAIQLAKARKSVLLISTDPAHNLSDAFGQKFGKEARLVDGYSNLSAMEIDPNGSIQDLLASGEAQGEDPMAGLGMGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSSQFGPMLNSI 196

HSP 2 Score: 36.965 bits (84), Expect = 2.315e-47
Identity = 15/30 (50.00%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFK+PD+TTFV
Sbjct:  210 DELLQKMESLRETISEVNTQFKNPDMTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_PENCW (ATPase get3 OS=Penicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) GN=get3 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.017e-47
Identity = 91/183 (49.73%), Postives = 126/183 (68.85%), Query Frame = 2

Query:  104 EGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEE--ETGSTEGMD--------SLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            E +++++L Q++L+W+F            S  L+I LA+VR SVL+ISTDPAHNLSDAF Q+F K   L++G+SNL AME+DP  S+++   TG  +G D        ++  +LA +IPG+DEAMSFAE+LK V+++ Y  IVFDTAPTGHTLR LQFP+ LEK L K+  L  +FG M+N +
Sbjct:   14 EPTLQSVLNQNTLRWIFVGGKGGVGKTTTSCSLAIQLAKVRKSVLLISTDPAHNLSDAFGQKFGKEARLIDGYSNLSAMEIDPNGSIQDLLATGDGQGEDPMAGLGMGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIVFDTAPTGHTLRFLQFPTVLEKALAKLSQLSTQFGPMLNSI 196

HSP 2 Score: 36.965 bits (84), Expect = 3.017e-47
Identity = 15/30 (50.00%), Postives = 22/30 (73.33%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D LL ++  +++ I  VN QFK+PD+TTFV
Sbjct:  210 DELLQKMESLRETISEVNTQFKNPDMTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_PARBP (ATPase GET3 OS=Paracoccidioides brasiliensis (strain Pb03) GN=GET3 PE=3 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.933e-47
Identity = 92/183 (50.27%), Postives = 125/183 (68.31%), Query Frame = 2

Query:  104 EGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEE--ETGSTEGMDSL--------FSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            E +++N+L+Q +L+WVF            S  L+I LA+VR SVL+ISTDPAHNLSDAF Q+F K   L++GF+NL AME+DP  S+++    G  +G DS+          +LA +IPG+DEAMSFAE+LK V+++ Y  I+FDTAPTGHTLR LQFP+ LEK L K+  L  +FG M+N +
Sbjct:   14 EPTLQNLLDQKTLRWVFVGGKGGVGKTTTSCSLAIQLAKVRKSVLLISTDPAHNLSDAFGQKFGKEARLIDGFTNLSAMEIDPNGSIQDLLAAGGGQGDDSMGGLGIGGMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIIFDTAPTGHTLRFLQFPTVLEKALAKLAQLSTQFGPMLNSI 196

HSP 2 Score: 36.1946 bits (82), Expect = 3.933e-47
Identity = 15/30 (50.00%), Postives = 21/30 (70.00%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D +L ++  +++ I  VN QFKD DLTTFV
Sbjct:  210 DEILSKMESLRETIAEVNAQFKDADLTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_PARBD (ATPase GET3 OS=Paracoccidioides brasiliensis (strain Pb18) GN=GET3 PE=3 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.933e-47
Identity = 92/183 (50.27%), Postives = 125/183 (68.31%), Query Frame = 2

Query:  104 EGSVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEE--ETGSTEGMDSL--------FSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMINQM 616
            E +++N+L+Q +L+WVF            S  L+I LA+VR SVL+ISTDPAHNLSDAF Q+F K   L++GF+NL AME+DP  S+++    G  +G DS+          +LA +IPG+DEAMSFAE+LK V+++ Y  I+FDTAPTGHTLR LQFP+ LEK L K+  L  +FG M+N +
Sbjct:   14 EPTLQNLLDQKTLRWVFVGGKGGVGKTTTSCSLAIQLAKVRKSVLLISTDPAHNLSDAFGQKFGKEARLIDGFTNLSAMEIDPNGSIQDLLAAGGGQGDDSMGGLGIGGMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYEVIIFDTAPTGHTLRFLQFPTVLEKALAKLAQLSTQFGPMLNSI 196

HSP 2 Score: 36.1946 bits (82), Expect = 3.933e-47
Identity = 15/30 (50.00%), Postives = 21/30 (70.00%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D +L ++  +++ I  VN QFKD DLTTFV
Sbjct:  210 DEILSKMESLRETIAEVNAQFKDADLTTFV 239

[Close]

BLAST of DR406179 vs. ExPASy Swiss-Prot
Match: GET3_PYRTR (ATPase get3 OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) GN=get3 PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 6.681e-47
Identity = 92/180 (51.11%), Postives = 123/180 (68.33%), Query Frame = 2

Query:  110 SVRNILEQDSLKWVFXXXXXXXXXXXCSSILSILLAEVRPSVLIISTDPAHNLSDAFQQRFTKTPTLVNGFSNLYAMEVDP--SVEE--ETGSTEGMD---------SLFSELANAIPGIDEAMSFAEMLKLVQTMDYSCIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKNKFGGMIN 610
            ++++IL+Q +L+W+F            S  L+I LA+ R SVL+ISTDPAHNLSDAF Q+F K   LVNGF NL AME+DP  S+++   +G+ EG D         S+  +LA +IPG+DEAMSFAE+LK V++M Y  I+FDTAPTGHTLR LQFP+ +EK L K+  L  +FG M+N
Sbjct:   14 TLQSILDQKTLRWIFVGGKGGVGKTTTSCSLAIQLAKHRKSVLLISTDPAHNLSDAFNQKFGKDARLVNGFDNLSAMEIDPNGSIQDLLASGAEEGQDPMAGLGGMGSMMQDLAFSIPGVDEAMSFAEVLKQVKSMSYEVIIFDTAPTGHTLRFLQFPTVMEKALSKVSQLSRQFGPMLN 193

HSP 2 Score: 34.6538 bits (78), Expect = 6.681e-47
Identity = 15/30 (50.00%), Postives = 21/30 (70.00%), Query Frame = 3

Query:  654 DALLGRLXXMKDVIERVNKQFKDPDLTTFV 743
            D L+ ++  +++ I  VN QFKD DLTTFV
Sbjct:  209 DELIEKMENLRETIGEVNGQFKDADLTTFV 238

The following BLAST results are available for this feature:

BLAST of DR406179 vs. ExPASy Swiss-Prot

BLAST of DR406179 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 111


Match Name	E-value	Identity	Description
GET3_ASPFC	1.046e-47	49.46	ATPase get3 OS=Aspergillus fumigatus (strain CEA10...	[more]
GET3_ASPOR	1.363e-47	48.92	ATPase get3 OS=Aspergillus oryzae GN=get3 PE=3 SV=...	[more]
GET3_ASPFN	1.363e-47	48.92	ATPase get3 OS=Aspergillus flavus (strain ATCC 200...	[more]
GET3_ASPTN	1.776e-47	48.92	ATPase get3 OS=Aspergillus terreus (strain NIH 262...	[more]
GET3_PENMQ	2.315e-47	48.92	ATPase get3 OS=Penicillium marneffei (strain ATCC ...	[more]
GET3_ASPCL	2.315e-47	48.92	ATPase get3 OS=Aspergillus clavatus GN=get3 PE=3 S...	[more]
GET3_PENCW	3.017e-47	49.73	ATPase get3 OS=Penicillium chrysogenum (strain ATC...	[more]
GET3_PARBP	3.933e-47	50.27	ATPase GET3 OS=Paracoccidioides brasiliensis (stra...	[more]
GET3_PARBD	3.933e-47	50.27	ATPase GET3 OS=Paracoccidioides brasiliensis (stra...	[more]
GET3_PYRTR	6.681e-47	51.11	ATPase get3 OS=Pyrenophora tritici-repentis (strai...	[more]

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Properties


Property Name	Value
Genbank description	CSAJ-PNP1248P22 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248P22 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR406179 ID=DR406179; Name=DR406179; organism=Citrus sinensis; type=EST; length=745bp
GGGGAGAGAACAAGAGGCAGTGAGTGAGTTGAAAAAGAGGGAAAAAAGAA
AAGAAAAGATGACGGAGGAGGATCAAGATCAAGATCAAGAATTAGAAATT
CCAGAGGGCAGTGTACGGAATATACTAGAACAAGATAGTCTCAAGTGGGT
CTTCGTCGGAGGCAAAGGCGGCGTTGGCAAAACGACATGTAGTTCAATTC
TCTCGATCCTTTTGGCCGAGGTCCGACCTTCTGTCTTGATCATATCCACT
GACCCAGCTCACAATCTCAGCGATGCTTTTCAGCAACGCTTCACCAAAAC
ACCTACTTTAGTCAATGGTTTCTCCAATCTTTATGCCATGGAGGTGGATC
CTAGTGTCGAAGAAGAAACGGGTTCTACCGAGGGAATGGATAGCTTGTTT
TCTGAGCTAGCAAATGCTATTCCCGGTATTGATGAGGCAATGAGCTTTGC
GGAGATGCTCAAATTGGTGCAAACAATGGATTATTCTTGTATAGTATTTG
ATACAGCTCCAACTGGCCATACACTCCGGCTATTACAATTTCCTTCAACC
TTAGAGAAGGGGCTAGACAAAATGATGTCCTTGAAAAATAAATTTGGCGG
CATGATTAATCAGATGACCCGTCTCTTTGGAATTGATGATGANATTTGGG
GAGGATGCTCTCTTAGGAAGGCTTGNNNGNATGAAAGATGTGATTGAACG
AGTTAATAAGCAGTTCAAGGACCCAGACTTGACAACCTTTGTCTG