DR403392

Overview
NameDR403392
Unique NameDR403392
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length738
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig6088 contig Csv1_Contig6088:1..738. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_CAEEL (Probable 26S protease regulatory subunit S10B OS=Caenorhabditis elegans GN=rpt-4 PE=1 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 2.570e-29
Identity = 66/185 (35.68%), Postives = 117/185 (63.24%), Query Frame = 2 
Query:  191 EDDLYSRLKSLQRQL-EFIDIQEEY--VKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            ED+    L S +R+L E  DI+++   ++ ++  + ++  +++ ++K +QSV  ++G+ ++ + +   IV +T G  Y V    +IN+E LK    V+L   +  ++  LP E D  +  +S  +  +++Y+D+GG   Q +E+RE VELPL + EL+K++GI PP+G LL+GPPGTGKT+LA+A
Sbjct:   19 EDERTKALNSYRRKLAECRDIEQKLKDLRKKESEMTKQFDKSENDIKSLQSVGQIVGEVLKQLSEEKFIVKATNGPRYVVGCRRSINKEELKQGTRVSLDMTTLTIMRQLPREVDPLVYKMSHEDPGNISYSDVGGLAEQIRELREVVELPLINPELFKRVGITPPKGCLLFGPPGTGKTLLARA 203
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_SPETR (26S protease regulatory subunit S10B OS=Spermophilus tridecemlineatus GN=PSMC6 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.725e-29
Identity = 67/200 (33.50%), Postives = 116/200 (58.00%), Query Frame = 2 
Query:  137 VSEPPPPTRSDLTEQWSDEDDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++P      D  ++  +  ++  RLK L+ QL              K L ++  +++ ++K +QSV  ++G+ ++ + +   IV +T G  Y V     +++  LKP   VAL   +  ++  LP E D  +  +S  +  +V+Y++IGG   Q +E+RE +ELPLT+ EL++++GI PP+G LLYGPPGTGKT+LA+A
Sbjct:    1 MADPRDKALQDYRKKLLEHKEIDGRLKELREQL--------------KELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARA 186
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_MOUSE (26S protease regulatory subunit S10B OS=Mus musculus GN=Psmc6 PE=1 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.725e-29
Identity = 67/200 (33.50%), Postives = 116/200 (58.00%), Query Frame = 2 
Query:  137 VSEPPPPTRSDLTEQWSDEDDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++P      D  ++  +  ++  RLK L+ QL              K L ++  +++ ++K +QSV  ++G+ ++ + +   IV +T G  Y V     +++  LKP   VAL   +  ++  LP E D  +  +S  +  +V+Y++IGG   Q +E+RE +ELPLT+ EL++++GI PP+G LLYGPPGTGKT+LA+A
Sbjct:    1 MADPRDKALQDYRKKLLEHKEIDGRLKELREQL--------------KELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARA 186
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_HUMAN (26S protease regulatory subunit S10B OS=Homo sapiens GN=PSMC6 PE=1 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.725e-29
Identity = 67/200 (33.50%), Postives = 116/200 (58.00%), Query Frame = 2 
Query:  137 VSEPPPPTRSDLTEQWSDEDDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++P      D  ++  +  ++  RLK L+ QL              K L ++  +++ ++K +QSV  ++G+ ++ + +   IV +T G  Y V     +++  LKP   VAL   +  ++  LP E D  +  +S  +  +V+Y++IGG   Q +E+RE +ELPLT+ EL++++GI PP+G LLYGPPGTGKT+LA+A
Sbjct:    1 MADPRDKALQDYRKKLLEHKEIDGRLKELREQL--------------KELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARA 186
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_BOVIN (26S protease regulatory subunit S10B OS=Bos taurus GN=PSMC6 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.725e-29
Identity = 67/200 (33.50%), Postives = 116/200 (58.00%), Query Frame = 2 
Query:  137 VSEPPPPTRSDLTEQWSDEDDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++P      D  ++  +  ++  RLK L+ QL              K L ++  +++ ++K +QSV  ++G+ ++ + +   IV +T G  Y V     +++  LKP   VAL   +  ++  LP E D  +  +S  +  +V+Y++IGG   Q +E+RE +ELPLT+ EL++++GI PP+G LLYGPPGTGKT+LA+A
Sbjct:    1 MADPRDKALQDYRKKLLEHKEIDGRLKELREQL--------------KELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARA 186
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PS6AB_ARATH (26S protease regulatory subunit 6A homolog B OS=Arabidopsis thaliana GN=RPT5B PE=1 SV=3)

HSP 1 Score: 122.479 bits (306), Expect = 2.405e-27
Identity = 62/202 (30.69%), Postives = 115/202 (56.93%), Query Frame = 2 
Query:  194 DDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVD------------------QNNG---IVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            DD+    + L  ++  +  + +    + +++K ++   QE++K  + +P ++G  +E+++                  Q  G   ++ ++T    ++ ++  ++ + LKP   V +++ S  ++D LP E DS +  +   EKP   YNDIGG + Q QE+ EA+ LP+TH E ++++GI PP+GVLLYGPPGTGKT++A+A
Sbjct:   22 DDIGRASRLLANEIRILKEESQRTNLDLESVKEKIKENQEKIKLNKQLPYLVGNIVEILEMSPEDDAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDTLKPGDLVGVNKDSYLILDTLPSEYDSRVKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKEQFEKLGIRPPKGVLLYGPPGTGKTLMARA 223
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS6A_RAT (26S protease regulatory subunit 6A OS=Rattus norvegicus GN=Psmc3 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 5.358e-27
Identity = 61/201 (30.35%), Postives = 114/201 (56.72%), Query Frame = 2 
Query:  194 DDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVD-----------------QNNG---IVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++  R + L  +++ +  +   V  E + +K ++    E++K  +++P ++   +E++D                 Q  G   ++ ++T   Y++ ++  ++ E LKP   V +++ S  +++ LP E DS +  +   E+P   Y+DIGG D Q QE+ EA+ LP+ H E ++ +GI PP+GVL+YGPPGTGKT+LA+A
Sbjct:   39 EEIVQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARA 239
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS6A_HUMAN (26S protease regulatory subunit 6A OS=Homo sapiens GN=PSMC3 PE=1 SV=3)

HSP 1 Score: 121.324 bits (303), Expect = 5.358e-27
Identity = 61/201 (30.35%), Postives = 114/201 (56.72%), Query Frame = 2 
Query:  194 DDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVD-----------------QNNG---IVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +++  R + L  +++ +  +   V  E + +K ++    E++K  +++P ++   +E++D                 Q  G   ++ ++T   Y++ ++  ++ E LKP   V +++ S  +++ LP E DS +  +   E+P   Y+DIGG D Q QE+ EA+ LP+ H E ++ +GI PP+GVL+YGPPGTGKT+LA+A
Sbjct:   39 EEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARA 239
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS6A_BRACM (26S protease regulatory subunit 6A homolog OS=Brassica campestris GN=TBP1 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 5.358e-27
Identity = 60/202 (29.70%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:  194 DDLYSRLKSLQRQLEFIDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNG---------------------IVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            +D+    + L+ ++  +    +    E  + K ++   QE++K  + +P ++G  +E+++ N                       ++ ++T    ++ ++  ++ + LKP   V +++ S  ++D LP E DS +  +   EKP   YNDIGG + Q QE+ EA+ LP+TH E ++++G+ PP+GVLLYGPPGTGKT++A+A
Sbjct:   23 EDIVRATRLLENEIRILKEDAQRTNLECDSYKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDDAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDSLKPGDLVGVNKDSYLILDTLPSEYDSRVKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKERFEKLGVRPPKGVLLYGPPGTGKTLMARA 224
[Close]
BLAST of DR403392 vs. ExPASy Swiss-Prot
Match: PRS10_YEAST (26S protease subunit RPT4 OS=Saccharomyces cerevisiae GN=RPT4 PE=1 SV=4)

HSP 1 Score: 120.553 bits (301), Expect = 9.140e-27
Identity = 58/164 (35.37%), Postives = 106/164 (64.63%), Query Frame = 2 
Query:  245 DIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKA 736
            D Q +  +   ++L++   + + ++K +QS+  +IG+ M+ + +   IV +++G  Y V + ++++R  LK    V L   +  ++ +LP E D  +  ++  E+ ++T++ IGG   Q +E+RE +ELPL + E+++++GI PP+GVLLYGPPGTGKT+LAKA
Sbjct:   71 DDQLKQRRQNIRDLEKLYDKTENDIKALQSIGQLIGEVMKELSEEKYIVKASSGPRYIVGVRNSVDRSKLKKGVRVTLDITTLTIMRILPRETDPLVYNMTSFEQGEITFDGIGGLTEQIRELREVIELPLKNPEIFQRVGIKPPKGVLLYGPPGTGKTLLAKA 234
The following BLAST results are available for this feature:
BLAST of DR403392 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 160
Match NameE-valueIdentityDescription
PRS10_CAEEL2.570e-2935.68Probable 26S protease regulatory subunit S10B OS=C... [more]
PRS10_SPETR5.725e-2933.5026S protease regulatory subunit S10B OS=Spermophil... [more]
PRS10_MOUSE5.725e-2933.5026S protease regulatory subunit S10B OS=Mus muscul... [more]
PRS10_HUMAN5.725e-2933.5026S protease regulatory subunit S10B OS=Homo sapie... [more]
PRS10_BOVIN5.725e-2933.5026S protease regulatory subunit S10B OS=Bos taurus... [more]
PS6AB_ARATH2.405e-2730.6926S protease regulatory subunit 6A homolog B OS=Ar... [more]
PRS6A_RAT5.358e-2730.3526S protease regulatory subunit 6A OS=Rattus norve... [more]
PRS6A_HUMAN5.358e-2730.3526S protease regulatory subunit 6A OS=Homo sapiens... [more]
PRS6A_BRACM5.358e-2729.7026S protease regulatory subunit 6A homolog OS=Bras... [more]
PRS10_YEAST9.140e-2735.3726S protease subunit RPT4 OS=Saccharomyces cerevis... [more]

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Properties
Property NameValue
Genbank descriptionCSAF-PNP1244N18 Flavedo Mature Citrus sinensis cDNA clone CSAF-PNP1244N18 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403392 ID=DR403392; Name=DR403392; organism=Citrus sinensis; type=EST; length=738bp
GGGGTCCTTTATTAAGAAAAAAAAATTTTGACAATCATCAATCGACCAAT
CCTTCGTTATAATTTCTTGCTGGTCACTCCAAATTCCTCTCAACAAAACA
ATGGCAGCCTCAGCGATGGTTCTAGACCCGAAGCCAGTATCGGAGCCACC
ACCGCCAACAAGATCCGATCTGACGGAGCAATGGTCGGACGAAGACGACC
TCTACAGCCGACTTAAATCCCTGCAACGGCAGCTCGAGTTCATCGACATC
CAAGAAGAGTACGTCAAAGACGAGCAAAAGAACTTGAAGCGTGAACTGCT
ACGCGCCCAGGAGGAGGTGAAGCGGATCCAATCGGTGCCGTTGGTAATCG
GACAGTTCATGGAGATGGTGGACCAGAATAACGGCATCGTGGGGTCCACC
ACCGGCTCTAACTACTACGTCAGGATTCTTAGCACTATTAATCGGGAGTT
GTTGAAACCGTCCGCTTCCGTCGCTCTCCACCGTCACTCTAACGCCCTTG
TTGACGTTCTTCCGCCGGAAGCCGATTCCAGTATTTCTCTTCTAAGCCAG
TCCGAGAAGCCTGACGTTACTTATAATGATATTGGAGGATGTGATATTCA
AAAGCAGGAAATTCGTGAGGCTGTGGAGTTACCGCTGACTCACCATGAGC
TATACAAGCAAATTGGTATAGATCCTCCACGTGGTGTTTTACTTTATGGT
CCTCCTGGAACTGGTAAAACTATGCTTGCAAAGGCTGT
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