DR403235

Overview
NameDR403235
Unique NameDR403235
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length388
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DR403235_ssr11 microsatellite DR403235_ssr11:11..26. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2998 contig Csv1_Contig2998:1061..1449. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_PICST (Autophagy-related protein 8 OS=Pichia stipitis GN=ATG8 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.587e-30
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DL+VGQFVYV+RKRIKL +EKAIFIFV +ILPPTAA++S IYEE+K
Sbjct:   46 KRKYLVPVDLSVGQFVYVIRKRIKLPSEKAIFIFVNDILPPTAALISTIYEEHK 99

HSP 2 Score: 65.0846 bits (157), Expect = 1.587e-30
Identity = 28/46 (60.87%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+RQAE+ RI +++ DR+PVI EK E SDIP+IDK+
Sbjct:    2 RSQFKDEHPFEKRQAEATRIAQRFKDRVPVICEKVENSDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_PICAN (Autophagy-related protein 8 OS=Pichia angusta GN=ATG8 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.599e-30
Identity = 40/54 (74.07%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+DLTVGQFVYV+RKRI+L +EKAIFIFV +ILPPTA++MS IYE+ K
Sbjct:   46 KRKYLVPSDLTVGQFVYVIRKRIQLPSEKAIFIFVNDILPPTASLMSTIYEQYK 99

HSP 2 Score: 66.6254 bits (161), Expect = 1.599e-30
Identity = 31/46 (67.39%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP ERR+AE++RIR K+ DRIPVI EK E SDIP+IDK+
Sbjct:    2 RSQFKDEHPFERRKAEASRIRGKFLDRIPVICEKVEESDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_YEAST (Autophagy-related protein 8 OS=Saccharomyces cerevisiae GN=ATG8 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.608e-30
Identity = 42/54 (77.78%), Postives = 49/54 (90.74%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTAA+MSAIY+E+K
Sbjct:   46 KRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHK 99

HSP 2 Score: 65.0846 bits (157), Expect = 1.608e-30
Identity = 29/46 (63.04%), Postives = 40/46 (86.96%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            KS+FK E+P E+R+AES RI +++ +RIPVI EKAE+SDIP+IDK+
Sbjct:    2 KSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_YEAS7 (Autophagy-related protein 8 OS=Saccharomyces cerevisiae (strain YJM789) GN=ATG8 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.608e-30
Identity = 42/54 (77.78%), Postives = 49/54 (90.74%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTAA+MSAIY+E+K
Sbjct:   46 KRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHK 99

HSP 2 Score: 65.0846 bits (157), Expect = 1.608e-30
Identity = 29/46 (63.04%), Postives = 40/46 (86.96%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            KS+FK E+P E+R+AES RI +++ +RIPVI EKAE+SDIP+IDK+
Sbjct:    2 KSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_CANGA (Autophagy-related protein 8 OS=Candida glabrata GN=ATG8 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.094e-30
Identity = 39/54 (72.22%), Postives = 48/54 (88.89%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVPADLTVGQFVYV+RKRI L  EKAIFIFV + LPPTA++MS +Y+E+K
Sbjct:   46 KRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTASLMSQVYQEHK 99

HSP 2 Score: 68.1662 bits (165), Expect = 2.094e-30
Identity = 31/46 (67.39%), Postives = 40/46 (86.96%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            KSSFK E+P E+R+AES RI EK+ +RIPVI EKAE+SDIP++DK+
Sbjct:    2 KSSFKSEYPFEKRKAESERISEKFQNRIPVICEKAEKSDIPEVDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_DEBHA (Autophagy-related protein 8 OS=Debaryomyces hansenii GN=ATG8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 1.009e-29
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DL+VGQFVYV+RKRIKL +EKAIFIF+ +ILPPTAA+MS IYEE+K
Sbjct:   46 KRKYLVPGDLSVGQFVYVIRKRIKLPSEKAIFIFINDILPPTAALMSTIYEEHK 99

HSP 2 Score: 61.2326 bits (147), Expect = 1.009e-29
Identity = 27/46 (58.70%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            +S FK EHP E+R+AE+ RI +++ DR+PVI EKAE SDI +IDK+
Sbjct:    2 RSQFKDEHPFEKRKAEATRITQRFNDRLPVICEKAENSDIQEIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_KLULA (Autophagy-related protein 8 OS=Kluyveromyces lactis GN=ATG8 PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.801e-29
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP DLTVGQFVYV+RKRIKL AEKAIFIFV + LPPTAA+MS+IY+++K
Sbjct:   46 KRKYLVPGDLTVGQFVYVIRKRIKLPAEKAIFIFVNDTLPPTAALMSSIYQQHK 99

HSP 2 Score: 60.077 bits (144), Expect = 3.801e-29
Identity = 28/46 (60.87%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            KS+FK E P E+R+AES RI +K+ +RIPVI E+  +SDIPDIDK+
Sbjct:    2 KSAFKSEFPFEKRKAESERIVQKFHNRIPVICERGGKSDIPDIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_ASHGO (Autophagy-related protein 8 OS=Ashbya gossypii GN=ATG8 PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.461e-29
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVPADLTVGQFVYV+R+RIKL AEKAIFIFV + LPPTAA+M A+Y+E+K
Sbjct:   45 RRKYLVPADLTVGQFVYVIRRRIKLPAEKAIFIFVNDTLPPTAALMFAVYQEHK 98

HSP 2 Score: 59.3066 bits (142), Expect = 6.461e-29
Identity = 25/45 (55.56%), Postives = 38/45 (84.44%), Query Frame = 3 
Query:   99 SSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            SSFK E+P E+R+AES RI  ++ +R+PVI E+AE+SDIP++D++
Sbjct:    2 SSFKSEYPFEKRKAESERICSQFENRVPVICERAEKSDIPEVDRR 46
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8_DICDI (Autophagy-related protein 8 OS=Dictyostelium discoideum GN=atg8 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.600e-27
Identity = 36/55 (65.45%), Postives = 47/55 (85.45%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRI-KLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KKYLVPAD+TVG+FVY +RK + K+SAEKAI++FV N +PPTAA++S IYE  K
Sbjct:   48 KKKYLVPADITVGKFVYEIRKHMTKVSAEKAIYLFVNNTIPPTAALISQIYERYK 102

HSP 2 Score: 66.6254 bits (161), Expect = 2.600e-27
Identity = 32/45 (71.11%), Postives = 37/45 (82.22%), Query Frame = 3 
Query:   99 SSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            SSFK +HPL++R+  + RIR KY DRIPVIVEKA RSD PDIDKK
Sbjct:    5 SSFKNDHPLDKRREVAERIRSKYLDRIPVIVEKAPRSDAPDIDKK 49
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRAP_RAT (Gamma-aminobutyric acid receptor-associated protein OS=Rattus norvegicus GN=Gabarap PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.364e-25
Identity = 32/53 (60.38%), Postives = 44/53 (83.02%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEN 385
            +KKYLVP+DLTVGQF +++RKRI L AE A+F FV N++PPT+A M  +Y+E+
Sbjct:   46 KKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEH 98

HSP 2 Score: 61.6178 bits (148), Expect = 1.364e-25
Identity = 27/46 (58.70%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  +K EHP E+R++E  +IR+KYPDR+PVIVEKA ++ I D+DKK
Sbjct:    2 KFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKK 47
The following BLAST results are available for this feature:
BLAST of DR403235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 72
Match NameE-valueIdentityDescription
ATG8_PICST1.587e-3075.93Autophagy-related protein 8 OS=Pichia stipitis GN=... [more]
ATG8_PICAN1.599e-3074.07Autophagy-related protein 8 OS=Pichia angusta GN=A... [more]
ATG8_YEAST1.608e-3077.78Autophagy-related protein 8 OS=Saccharomyces cerev... [more]
ATG8_YEAS71.608e-3077.78Autophagy-related protein 8 OS=Saccharomyces cerev... [more]
ATG8_CANGA2.094e-3072.22Autophagy-related protein 8 OS=Candida glabrata GN... [more]
ATG8_DEBHA1.009e-2975.93Autophagy-related protein 8 OS=Debaryomyces hansen... [more]
ATG8_KLULA3.801e-2975.93Autophagy-related protein 8 OS=Kluyveromyces lacti... [more]
ATG8_ASHGO6.461e-2975.93Autophagy-related protein 8 OS=Ashbya gossypii GN=... [more]
ATG8_DICDI2.600e-2765.45Autophagy-related protein 8 OS=Dictyostelium disco... [more]
GBRAP_RAT1.364e-2560.38Gamma-aminobutyric acid receptor-associated protei... [more]

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Properties
Property NameValue
Genbank descriptionCSAF-PNP1244E11 Flavedo Mature Citrus sinensis cDNA clone CSAF-PNP1244E11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403235 ID=DR403235; Name=DR403235; organism=Citrus sinensis; type=EST; length=388bp
GGGGAGTCTTTTTATTTATTTATTTATTTTAATCCGTCGATTCATATTCA
TTTTCCCCTTGGAAGCAGTAGAAAGAACAAGGAGAAGCTATGGCCAAGAG
CTCCTTCAAGCTTGAACACCCACTCGAAAGGAGGCAGGCTGAATCTGCCC
GTATCAGGGAGAAGTATCCTGATAGAATACCAGTGATTGTGGAGAAGGCT
GAAAGGAGTGACATACCTGATATTGACAAAAAAAATACCTGGTTCCTGCT
GATCTGACTGTTGGACAATTTGTTTATGTGGTCCGGAAGAGGATAAAGCT
CAGTGCTGAGAAGGCCATATTCATATTTGTCAAGAACATTTTGCCACCCA
CTGCTGCAATGATGTCTGCAATTTATGAGGAAAACAAA
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