DR403235

Overview
NameDR403235
Unique NameDR403235
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length388
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DR403235_ssr11 microsatellite DR403235_ssr11:11..26. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2998 contig Csv1_Contig2998:1061..1449. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL1_CAVPO (Gamma-aminobutyric acid receptor-associated protein-like 1 OS=Cavia porcellus GN=GABARAPL1 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.463e-24
Identity = 31/53 (58.49%), Postives = 42/53 (79.25%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEN 385
            ++KYLVP+DLTVGQF +++RKRI L  E A+F FV N +PPT+A M  +YE+N
Sbjct:   46 KRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDN 98

HSP 2 Score: 60.8474 bits (146), Expect = 1.463e-24
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  +K +HP E R+ E  +IR+KYPDR+PVIVEKA ++ +PD+DK+
Sbjct:    2 KFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL1_BOVIN (Gamma-aminobutyric acid receptor-associated protein-like 1 OS=Bos taurus GN=GABARAPL1 PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 1.463e-24
Identity = 31/53 (58.49%), Postives = 42/53 (79.25%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEN 385
            ++KYLVP+DLTVGQF +++RKRI L  E A+F FV N +PPT+A M  +YE+N
Sbjct:   46 KRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDN 98

HSP 2 Score: 60.8474 bits (146), Expect = 1.463e-24
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  +K +HP E R+ E  +IR+KYPDR+PVIVEKA ++ +PD+DK+
Sbjct:    2 KFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: LGG1_CAEEL (Protein lgg-1 OS=Caenorhabditis elegans GN=lgg-1 PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.674e-22
Identity = 28/53 (52.83%), Postives = 41/53 (77.36%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEN 385
            +KKYLVP+DLTVGQF +++RKRI+L  E A+F FV N++P T   M  +Y+++
Sbjct:   46 KKKYLVPSDLTVGQFYFLIRKRIQLRPEDALFFFVNNVIPQTMTTMGQLYQDH 98

HSP 2 Score: 57.7658 bits (138), Expect = 3.674e-22
Identity = 27/46 (58.70%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K ++K E+  E+R+AE  +IR KYPDRIPVIVEKA +S + D+DKK
Sbjct:    2 KWAYKEENNFEKRRAEGDKIRRKYPDRIPVIVEKAPKSKLHDLDKK 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL2_RAT (Gamma-aminobutyric acid receptor-associated protein-like 2 OS=Rattus norvegicus GN=Gabarapl2 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.265e-22
Identity = 27/54 (50.00%), Postives = 43/54 (79.63%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+D+TV QF++++RKRI+L +EKAIF+FV   +P ++  M  +YE+ K
Sbjct:   46 KRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEK 99

HSP 2 Score: 58.9214 bits (141), Expect = 6.265e-22
Identity = 29/46 (63.04%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  FK +H LE R  ESA+IR KYPDR+PVIVEK   S I DIDK+
Sbjct:    2 KWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL2_MOUSE (Gamma-aminobutyric acid receptor-associated protein-like 2 OS=Mus musculus GN=Gabarapl2 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.265e-22
Identity = 27/54 (50.00%), Postives = 43/54 (79.63%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+D+TV QF++++RKRI+L +EKAIF+FV   +P ++  M  +YE+ K
Sbjct:   46 KRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEK 99

HSP 2 Score: 58.9214 bits (141), Expect = 6.265e-22
Identity = 29/46 (63.04%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  FK +H LE R  ESA+IR KYPDR+PVIVEK   S I DIDK+
Sbjct:    2 KWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL2_HUMAN (Gamma-aminobutyric acid receptor-associated protein-like 2 OS=Homo sapiens GN=GABARAPL2 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.265e-22
Identity = 27/54 (50.00%), Postives = 43/54 (79.63%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+D+TV QF++++RKRI+L +EKAIF+FV   +P ++  M  +YE+ K
Sbjct:   46 KRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEK 99

HSP 2 Score: 58.9214 bits (141), Expect = 6.265e-22
Identity = 29/46 (63.04%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  FK +H LE R  ESA+IR KYPDR+PVIVEK   S I DIDK+
Sbjct:    2 KWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL2_BOVIN (Gamma-aminobutyric acid receptor-associated protein-like 2 OS=Bos taurus GN=GABARAPL2 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.265e-22
Identity = 27/54 (50.00%), Postives = 43/54 (79.63%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP+D+TV QF++++RKRI+L +EKAIF+FV   +P ++  M  +YE+ K
Sbjct:   46 KRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEK 99

HSP 2 Score: 58.9214 bits (141), Expect = 6.265e-22
Identity = 29/46 (63.04%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  FK +H LE R  ESA+IR KYPDR+PVIVEK   S I DIDK+
Sbjct:    2 KWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: GBRL3_HUMAN (Gamma-aminobutyric acid receptor-associated protein-like 3 OS=Homo sapiens GN=GABARAPL3 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.380e-21
Identity = 29/53 (54.72%), Postives = 40/53 (75.47%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEN 385
            ++KYLVP+DLT GQF  ++RKRI L  E A+F FV N +PPT+A M  +YE++
Sbjct:   46 RRKYLVPSDLTDGQFYLLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDS 98

HSP 2 Score: 56.9954 bits (136), Expect = 1.380e-21
Identity = 23/46 (50.00%), Postives = 35/46 (76.09%), Query Frame = 3 
Query:   96 KSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            K  +K  HP E R+ E  +IR+KYPDR+P+IVEKA ++ +PD+D++
Sbjct:    2 KFQYKEVHPFEYRKKEGEKIRKKYPDRVPLIVEKAPKARVPDLDRR 47
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8I_ARATH (Autophagy-related protein 8i OS=Arabidopsis thaliana GN=ATG8I PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.356e-20
Identity = 29/54 (53.70%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            +KK+LVP D++VGQF+Y++  R+ LS  KA+F+FV N LP TAA+M ++YE  K
Sbjct:   45 KKKFLVPRDMSVGQFIYILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYK 98

HSP 2 Score: 48.521 bits (114), Expect = 9.356e-20
Identity = 24/44 (54.55%), Postives = 31/44 (70.45%), Query Frame = 3 
Query:  102 SFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            SFK ++ L+ R AES  I  KYP RIPVI EK  ++D+P I+KK
Sbjct:    3 SFKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKK 46
[Close]
BLAST of DR403235 vs. ExPASy Swiss-Prot
Match: ATG8D_ORYSJ (Autophagy-related protein 8D OS=Oryza sativa subsp. japonica GN=ATG8D PE=3 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 5.861e-19
Identity = 26/54 (48.15%), Postives = 40/54 (74.07%), Query Frame = 2 
Query:  227 QKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEENK 388
            ++KYLVP ++ VGQF++++R R+ LS   A+F+FV N LP TA +M ++YE  K
Sbjct:   47 KRKYLVPCNMPVGQFIFILRSRLHLSPGTALFVFVNNTLPQTAQLMGSVYESYK 100

HSP 2 Score: 50.8322 bits (120), Expect = 5.861e-19
Identity = 25/48 (52.08%), Postives = 32/48 (66.67%), Query Frame = 3 
Query:   90 MAKSSFKLEHPLERRQAESARIREKYPDRIPVIVEKAERSDIPDIDKK 233
            M    FK E  LE R  ESA +   YPDRIPVIVEK  RS++P+++K+
Sbjct:    1 MKPRPFKEEFTLEERAKESAAMIASYPDRIPVIVEKFSRSNLPEMEKR 48
The following BLAST results are available for this feature:
BLAST of DR403235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 72
Match NameE-valueIdentityDescription
GBRL1_CAVPO1.463e-2458.49Gamma-aminobutyric acid receptor-associated protei... [more]
GBRL1_BOVIN1.463e-2458.49Gamma-aminobutyric acid receptor-associated protei... [more]
LGG1_CAEEL3.674e-2252.83Protein lgg-1 OS=Caenorhabditis elegans GN=lgg-1 P... [more]
GBRL2_RAT6.265e-2250.00Gamma-aminobutyric acid receptor-associated protei... [more]
GBRL2_MOUSE6.265e-2250.00Gamma-aminobutyric acid receptor-associated protei... [more]
GBRL2_HUMAN6.265e-2250.00Gamma-aminobutyric acid receptor-associated protei... [more]
GBRL2_BOVIN6.265e-2250.00Gamma-aminobutyric acid receptor-associated protei... [more]
GBRL3_HUMAN1.380e-2154.72Gamma-aminobutyric acid receptor-associated protei... [more]
ATG8I_ARATH9.356e-2053.70Autophagy-related protein 8i OS=Arabidopsis thalia... [more]
ATG8D_ORYSJ5.861e-1948.15Autophagy-related protein 8D OS=Oryza sativa subsp... [more]

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Properties
Property NameValue
Genbank descriptionCSAF-PNP1244E11 Flavedo Mature Citrus sinensis cDNA clone CSAF-PNP1244E11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403235 ID=DR403235; Name=DR403235; organism=Citrus sinensis; type=EST; length=388bp
GGGGAGTCTTTTTATTTATTTATTTATTTTAATCCGTCGATTCATATTCA
TTTTCCCCTTGGAAGCAGTAGAAAGAACAAGGAGAAGCTATGGCCAAGAG
CTCCTTCAAGCTTGAACACCCACTCGAAAGGAGGCAGGCTGAATCTGCCC
GTATCAGGGAGAAGTATCCTGATAGAATACCAGTGATTGTGGAGAAGGCT
GAAAGGAGTGACATACCTGATATTGACAAAAAAAATACCTGGTTCCTGCT
GATCTGACTGTTGGACAATTTGTTTATGTGGTCCGGAAGAGGATAAAGCT
CAGTGCTGAGAAGGCCATATTCATATTTGTCAAGAACATTTTGCCACCCA
CTGCTGCAATGATGTCTGCAATTTATGAGGAAAACAAA
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