DN621537

Overview
NameDN621537
Unique NameDN621537
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length749
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_XANOM (3-isopropylmalate dehydrogenase OS=Xanthomonas oryzae pv. oryzae (strain MAFF 311018) GN=leuB PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 6.703e-49
Identity = 96/152 (63.16%), Postives = 119/152 (78.29%), Query Frame = -2 
Query:  293 ITAIASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 748
            +  +A +YPD+ LSHMYVDNAAMQL+R PKQFD IVT+N+FGDILSD+ASM+TGSIGMLPSASL  +  G++EP HGSAPDIAG+  ANPLATILS AM+L+Y   +  AA  IE+AV   LD+GLRT DI+S GT  VG   MG+ V+ ++
Sbjct:  206 VEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVIVTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL 357
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_XANC5 (3-isopropylmalate dehydrogenase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=leuB PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 6.703e-49
Identity = 96/152 (63.16%), Postives = 119/152 (78.29%), Query Frame = -2 
Query:  293 ITAIASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 748
            +  +A +YPD+ LSHMYVDNAAMQL+R PKQFD IVT+N+FGDILSD+ASM+TGSIGMLPSASL  +  G++EP HGSAPDIAG+  ANPLATILS AM+L+Y   +  AA  IE+AV   LD+GLRT DI+S GT  VG   MG+ V+ ++
Sbjct:  206 VEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVIVTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL 357
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_METCA (3-isopropylmalate dehydrogenase OS=Methylococcus capsulatus GN=leuB PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 6.703e-49
Identity = 95/152 (62.50%), Postives = 120/152 (78.95%), Query Frame = -2 
Query:  293 ITAIASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 748
            +  +  +YPDV LSHMYVDNAAMQL+RNPKQFD ++T+N+FGDILSD A+M+TGSIGMLPSASL ESG G++EPIHGSAPDIAG+  ANP+ATILS AM+L+Y   +  +A+RI KAV   LD+G RT DI S GT  VG   MG+ ++ ++
Sbjct:  206 VIEVGKDYPDVALSHMYVDNAAMQLVRNPKQFDVMLTDNMFGDILSDCAAMLTGSIGMLPSASLAESGKGMYEPIHGSAPDIAGRGIANPIATILSLAMMLRYSFDDAVSAERIGKAVQTALDQGFRTADIASEGTVEVGTAAMGDAIVAAL 357
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_HELHP (3-isopropylmalate dehydrogenase OS=Helicobacter hepaticus GN=leuB PE=3 SV=2)

HSP 1 Score: 193.741 bits (491), Expect = 8.754e-49
Identity = 94/152 (61.84%), Postives = 120/152 (78.95%), Query Frame = -2 
Query:  293 ITAIASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 748
            +  +A  Y DV LS+MYVDNAAMQ+ R P QFD I+T N+FGDILSDEAS+ITG+IG++PSASL     G++EPIHGSAPDIAGQDKANP+ TILSAAM+L+   G    ++ I+KAV + LDKG RTGD+ S G +LVGC++MG+ +L+SV
Sbjct:  205 VDKVAQNYKDVHLSYMYVDNAAMQICRAPSQFDVILTENMFGDILSDEASIITGTIGVIPSASLSNGTLGMYEPIHGSAPDIAGQDKANPIGTILSAAMMLELSFGLTKESEVIQKAVQNALDKGYRTGDMMSEGMQLVGCEQMGDVILESV 356
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_BURPS (3-isopropylmalate dehydrogenase OS=Burkholderia pseudomallei GN=leuB PE=3 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 8.754e-49
Identity = 99/149 (66.44%), Postives = 116/149 (77.85%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            ++ EY DVELSHMYVDNAAMQL + PKQFD IVT N+FGDILSDEASM+TGSIGMLPSASL +   GL+EP HGSAPDIAG+  ANPLATILSAAMLL+Y L     A RIE+AV   L++G RTGDI + G K VG   MG+ V+ ++
Sbjct:  207 VSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERAVKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL 355
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_BURP1 (3-isopropylmalate dehydrogenase OS=Burkholderia pseudomallei (strain 1710b) GN=leuB PE=3 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 8.754e-49
Identity = 99/149 (66.44%), Postives = 116/149 (77.85%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            ++ EY DVELSHMYVDNAAMQL + PKQFD IVT N+FGDILSDEASM+TGSIGMLPSASL +   GL+EP HGSAPDIAG+  ANPLATILSAAMLL+Y L     A RIE+AV   L++G RTGDI + G K VG   MG+ V+ ++
Sbjct:  207 VSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERAVKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL 355
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_BURMA (3-isopropylmalate dehydrogenase OS=Burkholderia mallei GN=leuB PE=3 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 8.754e-49
Identity = 99/149 (66.44%), Postives = 116/149 (77.85%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            ++ EY DVELSHMYVDNAAMQL + PKQFD IVT N+FGDILSDEASM+TGSIGMLPSASL +   GL+EP HGSAPDIAG+  ANPLATILSAAMLL+Y L     A RIE+AV   L++G RTGDI + G K VG   MG+ V+ ++
Sbjct:  207 VSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERAVKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL 355
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_BURTA (3-isopropylmalate dehydrogenase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=leuB PE=3 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 1.143e-48
Identity = 98/149 (65.77%), Postives = 117/149 (78.52%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            ++ EY DVELSHMYVDNAAMQL + PKQFD IVT N+FGDILSDEASM+TGSIGMLPSASL ++  GL+EP HGSAPDIAG+  ANPLATILSAAMLL+Y L     A RIE+AV   L++G RTGDI + G + VG   MG+ V+ ++
Sbjct:  207 VSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERAVKTVLEQGYRTGDIATPGCRQVGTAAMGDAVVAAL 355
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_XYLFT (3-isopropylmalate dehydrogenase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=leuB PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.493e-48
Identity = 99/149 (66.44%), Postives = 115/149 (77.18%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            +A +YPDV LSHMYVDNAAMQL+R PKQFD IVT N+FGDILSD+ASM+TGSIGMLPSASL  +  G++EP HGSAPDIAGQ  ANPLATILS AMLL+YG+G    A  IE AV   LD GLRT DI+S G   VG   MG+ V+ ++
Sbjct:  209 MAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVIVTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVAMLLRYGMGYIKTADAIEYAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL 357
[Close]
BLAST of DN621537 vs. ExPASy Swiss-Prot
Match: LEU3_XYLFA (3-isopropylmalate dehydrogenase OS=Xylella fastidiosa GN=leuB PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.493e-48
Identity = 98/149 (65.77%), Postives = 115/149 (77.18%), Query Frame = -2 
Query:  293 IASEYPDVELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEHAAKRIEKAVLDTLDKGLRTGDIYSAGTKLVGCQEMGEEVLKSV 739
            +A +YPDV LSHMYVDNAAMQL+R+PKQFD IVT N+FGDILSD+ASM+TGSIGMLPSASL  +  G++EP HGSAPDIAGQ  ANPLATILS AMLL+Y +G    A  IE AV   LD GLRT DI+S G   VG   MG+ V+ ++
Sbjct:  209 MAKDYPDVTLSHMYVDNAAMQLVRHPKQFDVIVTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVAMLLRYSMGHIKTADAIEHAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL 357
The following BLAST results are available for this feature:
BLAST of DN621537 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 372
Match NameE-valueIdentityDescription
LEU3_XANOM6.703e-4963.163-isopropylmalate dehydrogenase OS=Xanthomonas ory... [more]
LEU3_XANC56.703e-4963.163-isopropylmalate dehydrogenase OS=Xanthomonas cam... [more]
LEU3_METCA6.703e-4962.503-isopropylmalate dehydrogenase OS=Methylococcus c... [more]
LEU3_HELHP8.754e-4961.843-isopropylmalate dehydrogenase OS=Helicobacter he... [more]
LEU3_BURPS8.754e-4966.443-isopropylmalate dehydrogenase OS=Burkholderia ps... [more]
LEU3_BURP18.754e-4966.443-isopropylmalate dehydrogenase OS=Burkholderia ps... [more]
LEU3_BURMA8.754e-4966.443-isopropylmalate dehydrogenase OS=Burkholderia ma... [more]
LEU3_BURTA1.143e-4865.773-isopropylmalate dehydrogenase OS=Burkholderia th... [more]
LEU3_XYLFT1.493e-4866.443-isopropylmalate dehydrogenase OS=Xylella fastidi... [more]
LEU3_XYLFA1.493e-4865.773-isopropylmalate dehydrogenase OS=Xylella fastidi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_06N15_f Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0006N15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN621537 ID=DN621537; Name=DN621537; organism=Citrus sinensis; type=EST; length=749bp
TCAATTTTAATTTGATTTTCTAATTTGCATCAACATTGAGACACCAGAGC
AAACTGTAAACAAAGTGCAAATTAAAAGACTTGCTTTTATTTTCCAAAAG
TTGTCACAATGATATTCCATGTTACAACAGCTCCAGATGAAGGCGGTTTC
AGAAAGACGGAAAAGATACAACACCAACTCAATATCTTATCAACCAAAAA
TCTACCACCCAAAAGATTACAAAGCCGTGATCCTATAGCATCCTTAGTGC
TGGTCCGATTTTAATTTAAACAGCAGCAGGAACTTGAGTCTCTACTGATT
TCAAAACCTCTTCGCCCATCTCCTGGCATCCTACTAATTTAGTCCCAGCA
GAGTATATGTCACCCGTTCGAAGCCCCTTATCTAGAGTATCTAAAACTGC
TTTCTCAATTCTCTTTGCAGCATGTTCCTCCCCTAAGCCATACTTCAAAA
GCATAGCAGCACTGAGTATAGTAGCCAATGGGTTTGCTTTATCCTGTCCA
GCAATATCGGGAGCAGAACCATGTATTGGTTCAAAGAGTCCAGGTCCCGA
TTCACCAAGACTGGCAGATGGGAGCATTCCTATACTTCCAGTAATCATTG
AAGCCTCATCAGATAAAATGTCACCAAATATGTTGTTTGTCACAATTGTA
TCAAACTGTTTTGGATTGCGAATAAGTTGCATTGCAGCGTTGTCGACATA
CATATGTGAGAGTTCAACATCAGGATACTCTGAGGCTATTGCCGTGATT
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