DN621283

Overview
NameDN621283
Unique NameDN621283
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length752
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_NEOFI (ATP-dependent RNA helicase eIF4A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=tif1 PE=3 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 1.705e-76
Identity = 140/181 (77.35%), Postives = 158/181 (87.29%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKEEWKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDM+Q  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD RM+ +I++FY+  IEE+P NVADL+
Sbjct:  218 KFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_EMENI (ATP-dependent RNA helicase eIF4A OS=Emericella nidulans GN=tif1 PE=3 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 1.705e-76
Identity = 140/181 (77.35%), Postives = 158/181 (87.29%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKEEWKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDM+Q  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD RM+ +I++FY+  IEE+P NVADL+
Sbjct:  241 KFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 421
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_COCIM (ATP-dependent RNA helicase eIF4A OS=Coccidioides immitis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 1.705e-76
Identity = 140/181 (77.35%), Postives = 159/181 (87.85%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKEEWKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HG+M+QN RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD RM+ +I++FY+  IEE+P NVADL+
Sbjct:  218 KFMRDPVRILVKKAELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLIARDFTVSAMHGEMEQNQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_BOTFB (ATP-dependent RNA helicase eIF4A OS=Botryotinia fuckeliana (strain B05.10) GN=tif1 PE=3 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 1.705e-76
Identity = 140/181 (77.35%), Postives = 158/181 (87.29%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKE+WKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDMDQ  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD RM+ +I++FY+  IEE+P NVADL+
Sbjct:  218 KFMRDPVRILVKKAELTLEGIKQFYIAVEKEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQGQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_CRYNE (ATP-dependent RNA helicase eIF4A OS=Cryptococcus neoformans GN=TIF1 PE=3 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 3.799e-76
Identity = 135/182 (74.18%), Postives = 159/182 (87.36%), Query Frame = -3 
Query:  205 RKFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 750
            +KFM  P+RILVK+DELTLEGI+QFY+NVEKEEWKLETLCDLYET+ ITQ+VIF +TRRKVDWLT ++  R  TVSA HGDM Q  R++IM+EFRSGSSRVLITTDLLARGIDVQQVSLVINYDLP+  ENY+HRIGR GRFGRKGVAINFV+ +D+ ML +I+ +YN  +EE+P NVADL+
Sbjct:  220 KKFMRDPIRILVKKDELTLEGIRQFYINVEKEEWKLETLCDLYETVTITQAVIFCSTRRKVDWLTQQLHDRQFTVSAMHGDMKQEEREVIMKEFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSSKENYIHRIGRGGRFGRKGVAINFVSNEDKNMLEEIETYYNTQVEEMPLNVADLI 401
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_SCLS1 (ATP-dependent RNA helicase eIF4A OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) GN=tif1 PE=3 SV=1)

HSP 1 Score: 284.263 bits (726), Expect = 4.962e-76
Identity = 139/181 (76.80%), Postives = 158/181 (87.29%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKE+WKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDMDQ  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT +D RM+ +I++FY+  IEE+P NVADL+
Sbjct:  218 KFMRDPVRILVKKAELTLEGIKQFYIAVEKEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQGQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTAEDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: IF4A_ASPFU (ATP-dependent RNA helicase eIF4A OS=Aspergillus fumigatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 4.200e-75
Identity = 138/178 (77.53%), Postives = 155/178 (87.08%), Query Frame = -3 
Query:  214 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVA 747
            KFM  PVRILVK+ ELTLEGIKQFY+ VEKEEWKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDM+Q  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD RM+ +I++FY+  IEE+P NVA
Sbjct:  218 KFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVA 395
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: FAL1_NEOFI (ATP-dependent RNA helicase fal1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=fal1 PE=3 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.035e-73
Identity = 134/181 (74.03%), Postives = 156/181 (86.19%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVR+LVKRDELTLEGIKQ+++ VEKEEWK +TLCDLY+TL ITQ+VIF NTRRKVDWLTDKMR  + TVS+ HG+M Q  RD IM++FR G+SRVLI+TD+ ARGIDVQQVSLVINYDLPT  ENY+HRIGRSGRFGRKGVAINFVT DD R+L DI+ +Y+  I+E+P NVADLL
Sbjct:  218 KFMTDPVRVLVKRDELTLEGIKQYFIAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPTNRENYIHRIGRSGRFGRKGVAINFVTSDDVRILRDIELYYSTQIDEMPMNVADLL 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: FAL1_EMENI (ATP-dependent RNA helicase fal1 OS=Emericella nidulans GN=fal1 PE=3 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.035e-73
Identity = 134/181 (74.03%), Postives = 156/181 (86.19%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVR+LVKRDELTLEGIKQ+++ VEKEEWK +TLCDLY+TL ITQ+VIF NTRRKVDWLTDKMR  + TVS+ HG+M Q  RD IM++FR G+SRVLI+TD+ ARGIDVQQVSLVINYDLPT  ENY+HRIGRSGRFGRKGVAINFVT DD R+L DI+ +Y+  I+E+P NVADLL
Sbjct:  218 KFMTDPVRVLVKRDELTLEGIKQYFIAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPTNRENYIHRIGRSGRFGRKGVAINFVTSDDVRILRDIELYYSTQIDEMPMNVADLL 398
[Close]
BLAST of DN621283 vs. ExPASy Swiss-Prot
Match: FAL1_ASPTN (ATP-dependent RNA helicase fal1 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=fal1 PE=3 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.035e-73
Identity = 134/181 (74.03%), Postives = 156/181 (86.19%), Query Frame = -3 
Query:  205 KFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 747
            KFM  PVR+LVKRDELTLEGIKQ+++ VEKEEWK +TLCDLY+TL ITQ+VIF NTRRKVDWLTDKMR  + TVS+ HG+M Q  RD IM++FR G+SRVLI+TD+ ARGIDVQQVSLVINYDLPT  ENY+HRIGRSGRFGRKGVAINFVT DD R+L DI+ +Y+  I+E+P NVADLL
Sbjct:  218 KFMTDPVRVLVKRDELTLEGIKQYFIAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPTNRENYIHRIGRSGRFGRKGVAINFVTSDDVRILRDIELYYSTQIDEMPMNVADLL 398
The following BLAST results are available for this feature:
BLAST of DN621283 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_NEOFI1.705e-7677.35ATP-dependent RNA helicase eIF4A OS=Neosartorya fi... [more]
IF4A_EMENI1.705e-7677.35ATP-dependent RNA helicase eIF4A OS=Emericella nid... [more]
IF4A_COCIM1.705e-7677.35ATP-dependent RNA helicase eIF4A OS=Coccidioides i... [more]
IF4A_BOTFB1.705e-7677.35ATP-dependent RNA helicase eIF4A OS=Botryotinia fu... [more]
IF4A_CRYNE3.799e-7674.18ATP-dependent RNA helicase eIF4A OS=Cryptococcus n... [more]
IF4A_SCLS14.962e-7676.80ATP-dependent RNA helicase eIF4A OS=Sclerotinia sc... [more]
IF4A_ASPFU4.200e-7577.53ATP-dependent RNA helicase eIF4A OS=Aspergillus fu... [more]
FAL1_NEOFI1.035e-7374.03ATP-dependent RNA helicase fal1 OS=Neosartorya fis... [more]
FAL1_EMENI1.035e-7374.03ATP-dependent RNA helicase fal1 OS=Emericella nidu... [more]
FAL1_ASPTN1.035e-7374.03ATP-dependent RNA helicase fal1 OS=Aspergillus ter... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_06I02_f Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0006I02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN621283 ID=DN621283; Name=DN621283; organism=Citrus sinensis; type=EST; length=752bp
TTTTTTTTGCAATGTGCATGACCCATATGATTAAATGGTCGGATATGTTT
GAGAACAACATTAATTTAAGACACTAGACGGAAAGGAAGAAGAAACCAAA
AAAAAAAAAAAAAGGGGAGAAAAGAACCACCACATTAAACAGTACTTGCA
AAATCAGGAAACCCCTTCTTGCCTCGACAAACTTACAAAAACAGAACCTC
CTCAAAGAAGGTCAGCAACGTTTGAAGGCAGCTCCTCAATGACCACGTTA
TAGAACTTTTGAATATCAAAAAGCATTCTCTCATCATCCCTTGTCACAAA
ATTAATGGCAACACCCTTTCTTCCAAATCGACCACTTCGTCCAATACGGT
GAAGGTAGTTTTCTGGTTGAGTCGGCAGATCATAATTTATGACAAGTGAA
ACTTGCTGCACATCAATACCACGAGCCAACAGATCAGTGGTGATGAGAAC
ACGAGAAGAACCAGAGCGAAATTCACGCATAATGATATCTCGGGTGTTCT
GATCCATGTCCCCATGAGTGGCAGAGACTGTATGGTCACGGCTTCGCATC
TTGTCCGTAAGCCAGTCGACCTTTCGCCGAGTGTTCACAAAGATGACACT
CTGGGTTATGGCCAGAGTCTCATAAAGATCACAGAGTGTTTCAAGCTTCC
ATTCTTCCTTCTCAACATTGACATAAAATTGCTTGATACCCTCAAGGGTG
AGTTCATCACGTTTAACCAAGATTCTCACAGGCTTGTTCATAAACTTCCT
TG
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