DN617483

Overview
NameDN617483
Unique NameDN617483
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length824
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK_OSTLU (S-adenosylmethionine synthetase OS=Ostreococcus lucimarinus (strain CCE9901) GN=METK-1 PE=3 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.873e-67
Identity = 132/185 (71.35%), Postives = 144/185 (77.84%), Query Frame = -3 
Query:  274 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDD--PDFTWEIVKPL 822
            ++IA DL   VIK V+P+KYLD  TIFHL PSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKS+VA+GLARRC+ Q+SYAIGV EPLSV VD+YGTGKIPD EIL  VK  FDFRPGM+   LDLKRGGN R+  TAAYGHFGRD+    FTWE V PL
Sbjct:  203 EKIAEDLMNEVIKKVVPEKYLDADTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYISRQAAKSVVAAGLARRCLFQISYAIGVAEPLSVHVDTYGTGKIPDSEILAKVKAAFDFRPGMMGKALDLKRGGNKRYQTTAAYGHFGRDEDLDIFTWEKVVPL 387
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK_OSTTA (S-adenosylmethionine synthetase OS=Ostreococcus tauri GN=METK PE=3 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 1.092e-66
Identity = 130/184 (70.65%), Postives = 144/184 (78.26%), Query Frame = -3 
Query:  274 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPD-FTWEIVKPL 822
            ++IA DL   VIK V+P+KYLD  TIFHL PSGRFVIGGP GDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQ AKSIVA+GLARR + Q+SYAIGV +PLS+ VD+YG+GKIPD EIL+ VKE FDFR GMI  +LDLKRGGN R+  TAAYGHFGRDD D FTWE V PL
Sbjct:  203 EKIAEDLMNEVIKKVVPEKYLDADTIFHLNPSGRFVIGGPDGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQVAKSIVAAGLARRALFQISYAIGVAQPLSIHVDTYGSGKIPDSEILEKVKEKFDFRAGMIGKSLDLKRGGNKRYQTTAAYGHFGRDDTDVFTWEKVVPL 386
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK_DICDI (S-adenosylmethionine synthetase OS=Dictyostelium discoideum GN=metK PE=1 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 1.429e-58
Identity = 118/184 (64.13%), Postives = 137/184 (74.46%), Query Frame = -3 
Query:  271 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLK 822
            ++I ADLK+ VIK V+P +YLDD TI+HL PSGRFVIGGP GD+GLTGRKIIID+Y            GKD TKVDRSGAY  R  AKS+VA+GLA RC+VQVSY+IGV +PLSVFVD+YGTGK  D EIL I+  NFD RPG +  +L L R     F KTA+ GHFGR+DPDFTWE VK LK
Sbjct:  203 EQIRADLKELVIKAVVPAQYLDDNTIYHLNPSGRFVIGGPMGDSGLTGRKIIIDSYGGWGAHGGGAFSGKDATKVDRSGAYAARWIAKSLVAAGLADRCLVQVSYSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNNFDLRPGCLMRDLQLTR---PIFQKTASGGHFGRNDPDFTWETVKELK 383
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK_SYNWW (S-adenosylmethionine synthetase OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=metK PE=3 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 3.410e-52
Identity = 101/176 (57.39%), Postives = 132/176 (75.00%), Query Frame = -3 
Query:  289 IAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 816
            I  D+ +HVIK VIP + +D  TIF++ P+GRFVIGGP GD+GLTGRKII+DTY            GKDPTKVDRS +Y++R  AK+IVA+GLA RC +QV+YAIGV  PLS+ V+++GTGKI D +I++++K+NFD RP  I  +LDL+R     + KTAAYGHFGR+D +FTWE
Sbjct:  210 IEKDIIEHVIKAVIPPEMIDAHTIFYVNPTGRFVIGGPQGDSGLTGRKIIVDTYGGMARHGGGAFSGKDPTKVDRSASYMLRYVAKNIVAAGLAERCEIQVAYAIGVANPLSINVNTFGTGKISDDKIIELIKKNFDLRPAAIIRDLDLRR---PIYRKTAAYGHFGREDAEFTWE 382
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.597e-52
Identity = 108/185 (58.38%), Postives = 131/185 (70.81%), Query Frame = -3 
Query:  268 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 822
            DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTY            GKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK+NFD RPG+I  +LDLK+     + +TAAYGHFGRD   F WE+ K LK+
Sbjct:  216 DEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 204.142 bits (518), Expect = 7.597e-52
Identity = 108/185 (58.38%), Postives = 131/185 (70.81%), Query Frame = -3 
Query:  268 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 822
            DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTY            GKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK+NFD RPG+I  +LDLK+     + +TAAYGHFGRD   F WE+ K LK+
Sbjct:  216 DEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.597e-52
Identity = 108/185 (58.38%), Postives = 131/185 (70.81%), Query Frame = -3 
Query:  268 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 822
            DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTY            GKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK+NFD RPG+I  +LDLK+     + +TAAYGHFGRD   F WE+ K LK+
Sbjct:  216 DEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.597e-52
Identity = 108/185 (58.38%), Postives = 131/185 (70.81%), Query Frame = -3 
Query:  268 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 822
            DE+   LK+ VIK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTY            GKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK+NFD RPG+I  +LDLK+     + +TAAYGHFGRD   F WE+ K LK+
Sbjct:  216 DEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK2_RAT (S-adenosylmethionine synthetase isoform type-2 OS=Rattus norvegicus GN=Mat2a PE=2 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 2.210e-51
Identity = 107/185 (57.84%), Postives = 130/185 (70.27%), Query Frame = -3 
Query:  268 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 822
            DE+   LK+ +IK V+P KYLD+ TI+HL PSGRFVIGGP GDAGLTGRKII+DTY            GKD TKVDRS AY  R  AKS+V  GL RR +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK NFD RPG+I  +LDLK+     + +TAAYGHFGRD   F WE+ K LK+
Sbjct:  216 DEMRDALKEKLIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKNNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395
[Close]
BLAST of DN617483 vs. ExPASy Swiss-Prot
Match: METK_STACT (S-adenosylmethionine synthetase OS=Staphylococcus carnosus (strain TM300) GN=metK PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 8.399e-51
Identity = 99/180 (55.00%), Postives = 129/180 (71.67%), Query Frame = -3 
Query:  283 DEIAADLKQHVIKPVIPDKYLDDKTIFHLTPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIV 822
            D+I  D+++HVI P++P   LDD+T F + P+GRFVIGGP GDAGLTGRKII+DTY            GKDPTKVDRS AY  R  AK+IVA+GLA +C VQ++YAIGV EP+S+ +D++GTGK+P+ ++++ V++NFD RP  I   LDLKR     + +TAAYGHFGR D    WE V
Sbjct:  210 DQIQEDIRKHVIDPIVPASLLDDETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGFARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEVQLAYAIGVAEPVSISIDTFGTGKVPESKLVEAVRDNFDLRPAGIIKMLDLKR---PIYKQTAAYGHFGRTDVALPWERV 386
The following BLAST results are available for this feature:
BLAST of DN617483 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_OSTLU2.873e-6771.35S-adenosylmethionine synthetase OS=Ostreococcus lu... [more]
METK_OSTTA1.092e-6670.65S-adenosylmethionine synthetase OS=Ostreococcus ta... [more]
METK_DICDI1.429e-5864.13S-adenosylmethionine synthetase OS=Dictyostelium d... [more]
METK_SYNWW3.410e-5257.39S-adenosylmethionine synthetase OS=Syntrophomonas ... [more]
METK2_PONAB7.597e-5258.38S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MOUSE7.597e-5258.38S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_MACFA7.597e-5258.38S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_HUMAN7.597e-5258.38S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK2_RAT2.210e-5157.84S-adenosylmethionine synthetase isoform type-2 OS=... [more]
METK_STACT8.399e-5155.00S-adenosylmethionine synthetase OS=Staphylococcus ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_01E07_r Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0001E07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN617483 ID=DN617483; Name=DN617483; organism=Citrus sinensis; type=EST; length=824bp
TTTTTTTTCAACTCAAAGATCTGCTCATTCTTGTTTTATTCATCAATAAA
ATTTCAGAACAATTCATATTCGCCTCTCAATTCATTATGCCTTGGATTGA
GAATCCATTAAACATATTTATTAAAAAAAAAAAAGGAAAAGAAAAAGAAA
GAAAGAAAGAAAACATTCATGTAACTGAAACAGAAGTAAAAAAGAAAAGT
AGACATATATAAGCACCAATGAGAGGCCAGCAGCTCATCCTTGATTAGTT
TAAGCTTGAAGTTTCTCCCACTTGAGTGGCTTAACAATCTCCCAAGTGAA
ATCAGGATCATCTCTGCCAAAGTGCCCATAAGCGGCAGTCTTCAAGAACC
TGCCGTTGCCACCCCTCTTGAGATCAAGATTAATAGAAATCATCCCAGGC
CTAAAATCAAAATTCTCCTTCACAATCTTCAAAATTTCCTTGTCAGGGAT
CTTCCCAGTCCCATAACTATCAACAAAAACTGACAAAGGCTCGGGCACAC
CAATAGCGTAAGAAACCTGAACAATGCATCTCCTGGCAAGCCCGCTTGCC
ACAATACTCTTAGCAGCTTGCCTTACAATGTAAGCACCGCTTCTATCAAC
CTTGGTAGGATCTTTCCCTGAGAAAGCCCCACCACCGTGTGCCCCCCAGC
CACCATAAGTATCAATAATAATCTTCCGGCCGGTGAGCCCAGCATCACCG
TGAGGGCCACCAATCACAAAACGGCCAGATGGGGTAAGATGGAAAATAGT
TTTGTCATCCAAGTACTTGTCAGGAATCACAGGCTTGATCACATGTTGCT
TGAGATCTGCAGCAATCTCATCAT
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