DN617427

Overview
NameDN617427
Unique NameDN617427
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length590
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5723 contig Csv1_Contig5723:1..592. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_STAA3 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain USA300) GN=pckA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.611e-22
Identity = 53/108 (49.07%), Postives = 65/108 (60.19%), Query Frame = -1 
Query:  264 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 587
            YA +L E +  H    +LVNTGW+GG YG G RI L +TR++++    G L  A YTK   FGL IP EIE VP  IL+P N WSD + YK     L   F+KNFE F
Sbjct:  408 YADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKF 515
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_STAA2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain JH1) GN=pckA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.611e-22
Identity = 53/108 (49.07%), Postives = 65/108 (60.19%), Query Frame = -1 
Query:  264 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 587
            YA +L E +  H    +LVNTGW+GG YG G RI L +TR++++    G L  A YTK   FGL IP EIE VP  IL+P N WSD + YK     L   F+KNFE F
Sbjct:  408 YADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKF 515
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_STAA1 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=pckA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.611e-22
Identity = 53/108 (49.07%), Postives = 65/108 (60.19%), Query Frame = -1 
Query:  264 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 587
            YA +L E +  H    +LVNTGW+GG YG G RI L +TR++++    G L  A YTK   FGL IP EIE VP  IL+P N WSD + YK     L   F+KNFE F
Sbjct:  408 YADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKF 515
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_LEPIN (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira interrogans GN=pckA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.611e-22
Identity = 52/109 (47.71%), Postives = 67/109 (61.47%), Query Frame = -1 
Query:  264 KYAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 590
            KYA +L EKM+KH    +L+NTG  GGSYG G R+ L  TR+IID I  G + ++ +   PVF +  P  I GV S ILDP   W+D  AY  T  KLG +F KNF+ +
Sbjct:  406 KYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVDSAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQY 514
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_LEPIC (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=pckA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.611e-22
Identity = 52/109 (47.71%), Postives = 67/109 (61.47%), Query Frame = -1 
Query:  264 KYAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 590
            KYA +L EKM+KH    +L+NTG  GGSYG G R+ L  TR+IID I  G + ++ +   PVF +  P  I GV S ILDP   W+D  AY  T  KLG +F KNF+ +
Sbjct:  406 KYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVDSAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQY 514
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJJ (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pckA PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 3.410e-22
Identity = 52/114 (45.61%), Postives = 72/114 (63.16%), Query Frame = -1 
Query:  246 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGFVNHKIG 587
            YA +L EK++KH    +LVNTGWSGGSYG G R+ +  TR  I+AI  G++ +  +    VF L IP  +EGV S +L+P NTW D  AY +T  KL  +F +NF+ + + K G
Sbjct:  401 YARLLGEKIEKHEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSIAKCEFENFEVFNLAIPKALEGVESTLLNPINTWLDKNAYAETRDKLAHMFVQNFKRYEDVKEG 514
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJ8 (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) GN=pckA PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 3.410e-22
Identity = 52/114 (45.61%), Postives = 72/114 (63.16%), Query Frame = -1 
Query:  246 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGFVNHKIG 587
            YA +L EK++KH    +LVNTGWSGGSYG G R+ +  TR  I+AI  G++ +  +    VF L IP  +EGV S +L+P NTW D  AY +T  KL  +F +NF+ + + K G
Sbjct:  401 YARLLGEKIEKHEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSIAKCEFENFEVFNLAIPKALEGVESTLLNPINTWLDKNAYTETRDKLAHMFVQNFKRYEDVKEG 514
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_METPB (Phosphoenolpyruvate carboxykinase [ATP] OS=Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) GN=pckA PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.453e-22
Identity = 43/111 (38.74%), Postives = 70/111 (63.06%), Query Frame = -1 
Query:  255 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGFVNH 587
            Y  +L   M +HG   WLVNTGW+GG  G+G R+ +  TRR++ A   G+L +  + + P FG  +P E+ GV +++L P  TW++  A+ +T  +L T+F++NF+ F +H
Sbjct:  412 YGNLLRALMAEHGVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQTEFRRDPYFGFSVPVEVAGVETQVLSPVETWTNKAAFAETATRLVTMFRENFKRFESH 522
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJD (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=pckA PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.453e-22
Identity = 52/114 (45.61%), Postives = 73/114 (64.04%), Query Frame = -1 
Query:  246 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGFVNHKIG 587
            YA +L EK++K+    +LVNTGWSGGSYG G R+ +  TR  I+AI  G++ +  +    VF L IP  +EGV S +L+P NTWSD  AY +T  KL  +F +NF+ + + K G
Sbjct:  401 YARLLGEKIEKYEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNPINTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEG 514
[Close]
BLAST of DN617427 vs. ExPASy Swiss-Prot
Match: PCKA_STAAW (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain MW2) GN=pckA PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 7.596e-22
Identity = 52/108 (48.15%), Postives = 65/108 (60.19%), Query Frame = -1 
Query:  264 YAAMLAEKMQKHGATGWLVNTGWSGGSYGSGNRIKLAFTRRIIDAIHCGTLLEASYTKTPVFGLEIPTEIEGVPSEILDPENTWSDMKAYKDTLLKLGTLFKKNFEGF 587
            YA +L E +  H    +LVNTGW+GG YG G RI L +TR++++    G L  A YTK   FGL IP +IE VP  IL+P N WSD + YK     L   F+KNFE F
Sbjct:  408 YADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKF 515
The following BLAST results are available for this feature:
BLAST of DN617427 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 281
Match NameE-valueIdentityDescription
PCKA_STAA32.611e-2249.07Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAA22.611e-2249.07Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAA12.611e-2249.07Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_LEPIN2.611e-2247.71Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]
PCKA_LEPIC2.611e-2247.71Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]
PCKA_CAMJJ3.410e-2245.61Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_CAMJ83.410e-2245.61Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_METPB4.453e-2238.74Phosphoenolpyruvate carboxykinase [ATP] OS=Methylo... [more]
PCKA_CAMJD4.453e-2245.61Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_STAAW7.596e-2248.15Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_01D03_f Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0001D03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN617427 ID=DN617427; Name=DN617427; organism=Citrus sinensis; type=EST; length=590bp
ATCTCTATAGCTAAAAATCCAAAAACTAAATGAATCCTTCTTGAGTTGTA
GTACGAGTGAAGTAAATAGTAGAAGCTCAACGTAGAGAAGCATATGGAGC
TTTATAAATTTAACAAACATATGACTACATTGCAAATTAAGTTATATCTT
ATTTCTTGGTGTCCCATAGGCACCAGATCGATCTTAAAGACTGATTAGAA
AAGAGGGCCGGCTGCAAGAATCTCGTCAGTAAGCTTGTTGTCCTCTCCAA
TCTTGTGATTCACAAAGCCCTCAAAATTCTTTTTGAAAAGGGTTCCTAGC
TTCAAGAGTGTATCCTTGTAGGCCTTCATGTCCGACCATGTATTTTCTGG
ATCCAGGATTTCTGAGGGCACTCCCTCTATCTCAGTTGGGATCTCAAGCC
CAAAAACTGGAGTCTTTGTGTAGCTTGCCTCCAAAAGAGTCCCACAATGT
ATTGCATCAATGATTCTTCTCGTAAAAGCTAATTTAATACGATTTCCTGA
CCCATAGCTTCCACCTGACCAGCCAGTGTTAACCAACCATCCAGTAGCCC
CATGCTTCTGCATTTTTTCAGCCAGCATGGCAGCATACTT
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