DN135157

Overview
NameDN135157
Unique NameDN135157
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length546
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13665 contig Csv1_Contig13665:1..527. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_SOLTU (ADP-ribosylation factor 1 OS=Solanum tuberosum PE=2 SV=2)

HSP 1 Score: 274.633 bits (701), Expect = 2.087e-73
Identity = 138/147 (93.88%), Postives = 141/147 (95.92%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MGLT +KLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDR+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGLTISKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF_CRYNE (ADP-ribosylation factor OS=Cryptococcus neoformans GN=ARF PE=1 SV=3)

HSP 1 Score: 265.003 bits (676), Expect = 1.654e-70
Identity = 131/147 (89.12%), Postives = 139/147 (94.56%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MGL+ +KL + LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQG+IFVVDSNDR+RI EAR+EL RML+EDELRDA+LLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGLSVSKLLNGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERITEAREELQRMLSEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_SCHPO (ADP-ribosylation factor 1 OS=Schizosaccharomyces pombe GN=arf1 PE=2 SV=2)

HSP 1 Score: 264.618 bits (675), Expect = 2.160e-70
Identity = 130/147 (88.44%), Postives = 138/147 (93.88%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MGL+ +KLF  LF K+EMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEY+NISFTVWDVGGQDKIRPLWRHYFQNTQG+IFVVDSNDR+RI EA +EL RMLNEDELRDA+LLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGLSISKLFQSLFGKREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF2_RAT (ADP-ribosylation factor 2 OS=Rattus norvegicus GN=Arf2 PE=2 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 6.283e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F KLF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVF NKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNVFEKLFKSLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELTRMLAEDELRDAVLLVFVNKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF2_MOUSE (ADP-ribosylation factor 2 OS=Mus musculus GN=Arf2 PE=1 SV=2)

HSP 1 Score: 263.077 bits (671), Expect = 6.283e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F KLF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVF NKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNVFEKLFKSLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELTRMLAEDELRDAVLLVFVNKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF2_BOVIN (ADP-ribosylation factor 2 OS=Bos taurus GN=ARF2 PE=2 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 6.283e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F KLF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVF NKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNVFEKLFKSLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELTRMLAEDELRDAVLLVFVNKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF_DUGJA (ADP-ribosylation factor OS=Dugesia japonica PE=2 SV=3)

HSP 1 Score: 262.692 bits (670), Expect = 8.206e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG   T L  RLF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RMLNEDELRDAVLLVFANKQDLPNAMN AEITD  GLHS
Sbjct:    1 MGNLVTHLLDRLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLNEDELRDAVLLVFANKQDLPNAMNPAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_LOCMI (ADP-ribosylation factor 1 OS=Locusta migratoria GN=ARF1 PE=2 SV=2)

HSP 1 Score: 262.692 bits (670), Expect = 8.206e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EAR+EL RML EDELRDAVLL+FANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNVFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIGEAREELMRMLAEDELRDAVLLIFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_DROME (ADP-ribosylation factor 1 OS=Drosophila melanogaster GN=Arf79F PE=1 SV=2)

HSP 1 Score: 262.692 bits (670), Expect = 8.206e-70
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EAR+EL RML EDELRDAVLL+FANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNVFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIGEAREELMRMLAEDELRDAVLLIFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF_AJECA (ADP-ribosylation factor OS=Ajellomyces capsulata GN=ARF PE=1 SV=3)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 128/147 (87.07%), Postives = 137/147 (93.20%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG+ F+KLF R++ KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQG+IFVVDSNDRDR+VEAR+EL RMLNEDELRDA+LLVFANKQDLPNAM+ AEIT   GL S
Sbjct:    1 MGMAFSKLFDRIWGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNIQFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNEDELRDALLLVFANKQDLPNAMSPAEITQQLGLQS 147
The following BLAST results are available for this feature:
BLAST of DN135157 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF1_SOLTU2.087e-7393.88ADP-ribosylation factor 1 OS=Solanum tuberosum PE=... [more]
ARF_CRYNE1.654e-7089.12ADP-ribosylation factor OS=Cryptococcus neoformans... [more]
ARF1_SCHPO2.160e-7088.44ADP-ribosylation factor 1 OS=Schizosaccharomyces p... [more]
ARF2_RAT6.283e-7089.80ADP-ribosylation factor 2 OS=Rattus norvegicus GN=... [more]
ARF2_MOUSE6.283e-7089.80ADP-ribosylation factor 2 OS=Mus musculus GN=Arf2 ... [more]
ARF2_BOVIN6.283e-7089.80ADP-ribosylation factor 2 OS=Bos taurus GN=ARF2 PE... [more]
ARF_DUGJA8.206e-7089.80ADP-ribosylation factor OS=Dugesia japonica PE=2 S... [more]
ARF1_LOCMI8.206e-7089.80ADP-ribosylation factor 1 OS=Locusta migratoria GN... [more]
ARF1_DROME8.206e-7089.80ADP-ribosylation factor 1 OS=Drosophila melanogast... [more]
ARF_AJECA1.072e-6987.07ADP-ribosylation factor OS=Ajellomyces capsulata G... [more]

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Properties
Property NameValue
Genbank descriptionwca07a08 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone wca07a08 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN135157 ID=DN135157; Name=DN135157; organism=Citrus sinensis; type=EST; length=546bp
GGGGATAACATATTTCNNNNTTGTTGTTAAGCTAATAAGCNNNGTCCTCC
ACCCCCCCCCTCCGCTCTCTCTCGCGTTCTCTGAAGTGATCCCCAGCCGG
AAGAATGGGGCTGACTTTCACAAAGCTGTTCAGCCGGCTCTTTGCCAAGA
AAGAGATGCGAATTCTAATGGTTGGTCTTGATGCTGCTGGTAAGACCACT
ATCTTGTACAAGCTCAAGCTTGGAGAGATTGTCACCACAATTCCTACCAT
TGGGTTTAATGTGGAGACCGTTGAATATAAGAACATCAGTTTCACTGTGT
GGGATGTTGGTGGCCAGGACAAGATTCGTCCCTTGTGGAGGCATTATTTC
CAGAACACTCAAGGTCTCATTTTTGTTGTGGACAGCAATGACAGAGACCG
TATTGTAGAAGCAAGGGATGAATTGCATAGGATGTTGAATGAGGATGAGC
TGCGAGATGCAGTGTTGCTTGTATTTGCTAACAAACAGGATCTTCCAAAT
GCAATGAATGCTGCTGAGATTACTGATANNNNNGGCCTCCACTCCC
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