DN135157

Overview
NameDN135157
Unique NameDN135157
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length546
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13665 contig Csv1_Contig13665:1..527. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_XENLA (ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf1 PE=2 SV=2)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNMFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_RAT (ADP-ribosylation factor 1 OS=Rattus norvegicus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_MOUSE (ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_MACFA (ADP-ribosylation factor 1 OS=Macaca fascicularis GN=ARF1 PE=2 SV=3)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_HUMAN (ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 PE=1 SV=2)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_BOVIN (ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE=1 SV=2)

HSP 1 Score: 262.307 bits (669), Expect = 1.072e-69
Identity = 132/147 (89.80%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITD  GLHS
Sbjct:    1 MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF_NEUCR (ADP-ribosylation factor OS=Neurospora crassa GN=NCU08340 PE=3 SV=3)

HSP 1 Score: 261.536 bits (667), Expect = 1.828e-69
Identity = 129/143 (90.21%), Postives = 134/143 (93.71%), Query Frame = 3 
Query:  117 FTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            F KLF  LF KKEMRILMVGLDAAGKTTILYKLKLGE+VTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQG+IFVVDSNDRDR+VEAR+EL RMLNEDELRDA+LLVFANKQDLPNAMNAAEITD  GL S
Sbjct:    8 FGKLFDGLFGKKEMRILMVGLDAAGKTTILYKLKLGEVVTTIPTIGFNVETVEYKNIQFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLSS 150
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF12_CAEBR (ADP-ribosylation factor 1-like 2 OS=Caenorhabditis briggsae GN=arf-1.2 PE=3 SV=3)

HSP 1 Score: 259.225 bits (661), Expect = 9.073e-69
Identity = 130/147 (88.44%), Postives = 134/147 (91.16%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MG  F  LF  LF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLP AMNAAE+TD  GLHS
Sbjct:    1 MGNVFGSLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVGEAREELMRMLAEDELRDAVLLVFANKQDLPQAMNAAEVTDKLGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF_CANAL (ADP-ribosylation factor OS=Candida albicans GN=ARF1 PE=3 SV=4)

HSP 1 Score: 258.84 bits (660), Expect = 1.185e-68
Identity = 128/147 (87.07%), Postives = 137/147 (93.20%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MGLT +KLF+ L  ++EMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWR+YFQNTQG+IFVVDSNDRDRI EAR+EL  MLNEDEL+DAVLLV ANKQDLPNAMNAAEIT+  GLHS
Sbjct:    1 MGLTISKLFASLLGRREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRYYFQNTQGIIFVVDSNDRDRINEAREELQSMLNEDELKDAVLLVLANKQDLPNAMNAAEITEKMGLHS 147
[Close]
BLAST of DN135157 vs. ExPASy Swiss-Prot
Match: ARF1_DICDI (ADP-ribosylation factor 1 OS=Dictyostelium discoideum GN=arfA PE=1 SV=3)

HSP 1 Score: 258.84 bits (660), Expect = 1.185e-68
Identity = 128/147 (87.07%), Postives = 135/147 (91.84%), Query Frame = 3 
Query:  105 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDXXGLHS 545
            MGL F KLFSR F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVE+KNI+FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EA DEL +MLNEDELRDAVLLVF NKQDLPNAM+ AE+TD   LHS
Sbjct:    1 MGLAFGKLFSRFFGKKDMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEFKNINFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEACDELTKMLNEDELRDAVLLVFCNKQDLPNAMSVAEVTDKLNLHS 147
The following BLAST results are available for this feature:
BLAST of DN135157 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF1_XENLA1.072e-6989.80ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf... [more]
ARF1_RAT1.072e-6989.80ADP-ribosylation factor 1 OS=Rattus norvegicus GN=... [more]
ARF1_MOUSE1.072e-6989.80ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 ... [more]
ARF1_MACFA1.072e-6989.80ADP-ribosylation factor 1 OS=Macaca fascicularis G... [more]
ARF1_HUMAN1.072e-6989.80ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 ... [more]
ARF1_BOVIN1.072e-6989.80ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE... [more]
ARF_NEUCR1.828e-6990.21ADP-ribosylation factor OS=Neurospora crassa GN=NC... [more]
ARF12_CAEBR9.073e-6988.44ADP-ribosylation factor 1-like 2 OS=Caenorhabditis... [more]
ARF_CANAL1.185e-6887.07ADP-ribosylation factor OS=Candida albicans GN=ARF... [more]
ARF1_DICDI1.185e-6887.07ADP-ribosylation factor 1 OS=Dictyostelium discoid... [more]

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Properties
Property NameValue
Genbank descriptionwca07a08 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone wca07a08 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN135157 ID=DN135157; Name=DN135157; organism=Citrus sinensis; type=EST; length=546bp
GGGGATAACATATTTCNNNNTTGTTGTTAAGCTAATAAGCNNNGTCCTCC
ACCCCCCCCCTCCGCTCTCTCTCGCGTTCTCTGAAGTGATCCCCAGCCGG
AAGAATGGGGCTGACTTTCACAAAGCTGTTCAGCCGGCTCTTTGCCAAGA
AAGAGATGCGAATTCTAATGGTTGGTCTTGATGCTGCTGGTAAGACCACT
ATCTTGTACAAGCTCAAGCTTGGAGAGATTGTCACCACAATTCCTACCAT
TGGGTTTAATGTGGAGACCGTTGAATATAAGAACATCAGTTTCACTGTGT
GGGATGTTGGTGGCCAGGACAAGATTCGTCCCTTGTGGAGGCATTATTTC
CAGAACACTCAAGGTCTCATTTTTGTTGTGGACAGCAATGACAGAGACCG
TATTGTAGAAGCAAGGGATGAATTGCATAGGATGTTGAATGAGGATGAGC
TGCGAGATGCAGTGTTGCTTGTATTTGCTAACAAACAGGATCTTCCAAAT
GCAATGAATGCTGCTGAGATTACTGATANNNNNGGCCTCCACTCCC
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