DC900317

Overview
NameDC900317
Unique NameDC900317
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length817
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1B_BOVIN (GTP-binding protein SAR1b OS=Bos taurus GN=SAR1B PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 5.576e-39
Identity = 82/156 (52.56%), Postives = 98/156 (62.82%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH  ARRVWK                  ER  ESK+            NVP L+LGNKID P A SEE LR   GL   TTGKG V+L + N RPLEVFMCS++++ GYG+GF+W++QYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETVANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGNVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1B_HUMAN (GTP-binding protein SAR1b OS=Homo sapiens GN=SAR1B PE=1 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 7.282e-39
Identity = 81/156 (51.92%), Postives = 98/156 (62.82%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH  ARRVWK                  ER  ESK+            NVP L+LGNKID P A SEE LR   GL   TTGKG ++L + N RPLEVFMCS++++ GYG+GF+W++QYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSISLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1B_CRIGR (GTP-binding protein SAR1b OS=Cricetulus griseus GN=SAR1B PE=1 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 7.282e-39
Identity = 82/156 (52.56%), Postives = 98/156 (62.82%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH  ARRVWK                  ER  ESK+            NVP L+LGNKID P A SEE LR   GL   TTGKG V+L + N RPLEVFMCS++++ GYG+GF+W++QYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHIQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1_CRYNE (Small COPII coat GTPase SAR1 OS=Cryptococcus neoformans GN=SAR1 PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.720e-38
Identity = 83/156 (53.21%), Postives = 101/156 (64.74%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLK++RL   QPT +PTSEEL+IG +KF  +DLGGH  ARR+W+                  ERFAESK              VPFL+LGNKID   A SEE+LR+ LGL   TTGKGK+ L D  +RP+EVFMCS+V + GYG+GF+WLSQYI
Sbjct:   37 LHMLKNDRLATLQPTLHPTSEELAIGNVKFTTYDLGGHIQARRLWRDYFPEVDGIVFLVDSADAERFAESKAELDSLLSIESLAQVPFLILGNKIDAYGAVSEEQLRHELGLYQ-TTGKGKIPLRD--IRPIEVFMCSVVMRQGYGEGFRWLSQYI 189
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1A_PONAB (GTP-binding protein SAR1a OS=Pongo abelii GN=SAR1A PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 8.052e-38
Identity = 81/156 (51.92%), Postives = 96/156 (61.54%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH+ ARRVWK                   R  ESK             NVP L+LGNKID   A SEE+LR   GL   TTGKG V L + N RP+EVFMCS++++ GYG+GF+WLSQYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1A_HUMAN (GTP-binding protein SAR1a OS=Homo sapiens GN=SAR1A PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 8.052e-38
Identity = 81/156 (51.92%), Postives = 96/156 (61.54%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH+ ARRVWK                   R  ESK             NVP L+LGNKID   A SEE+LR   GL   TTGKG V L + N RP+EVFMCS++++ GYG+GF+WLSQYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1_YARLI (Small COPII coat GTPase SAR1 OS=Yarrowia lipolytica GN=SAR1 PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.052e-37
Identity = 84/156 (53.85%), Postives = 98/156 (62.82%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLK++R+    PT +PTSEELSIG  KF  FDLGGH  ARRVWK                   RFAESK              VPFL+LGNKID+P A SE EL+  LGL   TTGKGKV L + N+RP+E+FMCSIV + GYGDG +WLSQY+
Sbjct:   37 LHMLKNDRMAVSNPTVHPTSEELSIGNCKFTTFDLGGHIQARRVWKDYFPEVNGIVFLVDAADPTRFAESKAELDSLLAIEQLKTVPFLILGNKIDMPQAVSELELKDALGLYQ-TTGKGKVPL-EGNIRPIEIFMCSIVMRQGYGDGIRWLSQYV 190
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1A_PIG (GTP-binding protein SAR1a OS=Sus scrofa GN=SAR1A PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.373e-37
Identity = 81/156 (51.92%), Postives = 96/156 (61.54%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH+ ARRVWK                   R  ESK             NVP L+LGNKID   A SEE+LR   GL   TTGKG V L + N RP+EVFMCS++++ GYG+GF+WLSQYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHPRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1A_BOVIN (GTP-binding protein SAR1a OS=Bos taurus GN=SAR1A PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.373e-37
Identity = 81/156 (51.92%), Postives = 96/156 (61.54%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLKD+RL QH PT +PTSEEL+I  + F  FDLGGH+ ARRVWK                   R  ESK             NVP L+LGNKID   A SEE+LR   GL   TTGKG V L + N RP+EVFMCS++++ GYG+GF+WLSQYI
Sbjct:   42 LHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHPRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197
[Close]
BLAST of DC900317 vs. ExPASy Swiss-Prot
Match: SAR1_NEOFI (Small COPII coat GTPase sar1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=sar1 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.794e-37
Identity = 82/156 (52.56%), Postives = 99/156 (63.46%), Query Frame = 2 
Query:    2 LHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKXXXXXXXXXXXXXXXXXKERFAESKKXXXXXXXXXXXXNVPFLVLGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNLADSNVRPLEVFMCSIVRKMGYGDGFKWLSQYI 469
            LHMLK++R+   QPT +PTSEEL+IG  +F  FDLGGHQ ARR+WK                  ERF ESK              VPFL+LGNKID P A SE+ELR+ LGL   TTGKGKV L    +RP+EVFMCS+V + GYG+G +WLSQY+
Sbjct:   37 LHMLKNDRVATLQPTAHPTSEELAIGNNRFTTFDLGGHQQARRLWKDYFPEVSGIVFLVDAKDHERFPESKAELDALLAMEELAKVPFLILGNKIDHPDAVSEDELRHQLGLYQ-TTGKGKVPL--EGIRPIEVFMCSVVMRQGYGEGIRWLSQYV 189
The following BLAST results are available for this feature:
BLAST of DC900317 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
SAR1B_BOVIN5.576e-3952.56GTP-binding protein SAR1b OS=Bos taurus GN=SAR1B P... [more]
SAR1B_HUMAN7.282e-3951.92GTP-binding protein SAR1b OS=Homo sapiens GN=SAR1B... [more]
SAR1B_CRIGR7.282e-3952.56GTP-binding protein SAR1b OS=Cricetulus griseus GN... [more]
SAR1_CRYNE4.720e-3853.21Small COPII coat GTPase SAR1 OS=Cryptococcus neofo... [more]
SAR1A_PONAB8.052e-3851.92GTP-binding protein SAR1a OS=Pongo abelii GN=SAR1A... [more]
SAR1A_HUMAN8.052e-3851.92GTP-binding protein SAR1a OS=Homo sapiens GN=SAR1A... [more]
SAR1_YARLI1.052e-3753.85Small COPII coat GTPase SAR1 OS=Yarrowia lipolytic... [more]
SAR1A_PIG1.373e-3751.92GTP-binding protein SAR1a OS=Sus scrofa GN=SAR1A P... [more]
SAR1A_BOVIN1.373e-3751.92GTP-binding protein SAR1a OS=Bos taurus GN=SAR1A P... [more]
SAR1_NEOFI1.794e-3752.56Small COPII coat GTPase sar1 OS=Neosartorya fische... [more]

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Properties
Property NameValue
Genbank descriptionDC900317 VSS Citrus sinensis cDNA clone VS28666 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900317 ID=DC900317; Name=DC900317; organism=Citrus sinensis; type=EST; length=817bp
GCTTCACATGTTGAAAGATGAGAGATTGGTTCAACATCAGCCAACACAGT
ATCCCACATCGGAAGAGCTTAGTATTGGGAAAATTAAGTTCAAGGCGTTT
GATTTGGGAGGCCATCAGATTGCTAGGAGAGTCTGGAAGGATTACTATGC
GATNGTTGATGCTGTGGTATACCTAGTGGATGCCTATGACAAAGAGAGGT
TTGCAGAATCAAAGAAAGAACTGGATGCACTCCTTTCTGATGAGGCATTG
GCCAATGTTCCCTTTCTTGTGTTAGGAAACAAGATTGACATCCCTTATGC
TGCTTCTGAGGAAGAGTTGCGTTACCACCTTGGCCTATCAAATTTCACCA
CTGGGAAGGGGAAGGTTAATCTAGCGGACTCAAATGTCCGTCCACTGGAG
GTGTTTATGTGTAGCATAGTTCGCAAAATGGGATATGGTGATGGTTTCAA
ATGGCTCTCTCAATACATTAAGTAGGGTAATGCTCAGGAAGTGTTATAGA
AGTAATGCAGATTTCAAAATTTCACCCATGCCTACTACACCCTGTTTCAC
CAAAAAAAAATTGTGTTTATCTACTTGTTTGATCTTTTAGTCTGTTAGTG
GGACTTGAACCAGAGGTACTGTGTTTTGAGTCTGTTTGGAAGGTGGTAGT
TATGTTACTGCTCTGTAAAATTTACTTGTGGATAGCAGGTCATTATCTGG
TATTGTAGTTTTGTCAAATTACTTGCACCTATTGTCATGTTCTGCTGTGG
AGCAGATATTAGAATTATTATTTGTTATTATTTTGTTAAGGGTTAATTTC
AAGTTCAAAACATCCCT
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