CN186026

Overview
NameCN186026
Unique NameCN186026
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length775
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.309e-64
Identity = 80/101 (79.21%), Postives = 90/101 (89.11%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DLQ+DPP  CSAGPV +D+FHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF TK++HPNINSNGSICLDIL+ QWSPALT+SK
Sbjct:    1 MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSK 101

HSP 2 Score: 83.9593 bits (206), Expect = 7.309e-64
Identity = 38/47 (80.85%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIAH YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC1_COLGL (Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletotrichum gloeosporioides GN=UBC1 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 7.309e-64
Identity = 79/101 (78.22%), Postives = 90/101 (89.11%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP+SCSAGPV ED+FHWQATIMGP DSPY+GGVF + IHFP DYPFKPPKV F T+++HPNINSNGSICLDIL++QWSPALTISK
Sbjct:    1 MAFKRINKELTDLGRDPPSSCSAGPVGEDLFHWQATIMGPGDSPYSGGVFFLAIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISK 101

HSP 2 Score: 87.4261 bits (215), Expect = 7.309e-64
Identity = 37/47 (78.72%), Postives = 46/47 (97.87%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICS+LTDPNPD+PLVPEIAH+YK+D+A+YE+TAR WT+KYA+
Sbjct:  101 KVLLSICSMLTDPNPDEPLVPEIAHVYKTDRARYEATAREWTRKYAI 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.243e-63
Identity = 75/100 (75.00%), Postives = 94/100 (94.00%), Query Frame = -1 
Query:  467 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 766
            +SKRI +EL DL++DPPTSCSAGPV +D++HWQA+IMGP+DSPYAGGVF ++IHFP DYPFKPPK++F TK++HPNIN+NG+ICLDILK+QWSPALT+SK
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSK 102

HSP 2 Score: 85.8853 bits (211), Expect = 1.243e-63
Identity = 38/47 (80.85%), Postives = 44/47 (93.62%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLLTD NPDDPLVPEIAH+YK+D+ KYE+TAR WT+KYA+
Sbjct:  102 KVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV 148
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D2_XENLA (Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laevis GN=ube2d2 PE=1 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.039e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 81.6481 bits (200), Expect = 1.039e-62
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D2_RAT (Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norvegicus GN=Ube2d2 PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.039e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 81.6481 bits (200), Expect = 1.039e-62
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D2_MOUSE (Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus GN=Ube2d2 PE=1 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.039e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 81.6481 bits (200), Expect = 1.039e-62
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D2_HUMAN (Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens GN=UBE2D2 PE=1 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.039e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 81.6481 bits (200), Expect = 1.039e-62
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D2_BOVIN (Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.039e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 81.6481 bits (200), Expect = 1.039e-62
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 2.303e-62
Identity = 79/102 (77.45%), Postives = 89/102 (87.25%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MASKRI REL+D+Q+ PP +CSAGPVAE D+FHWQATIMGP DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SK
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSK 102

HSP 2 Score: 93.2041 bits (230), Expect = 2.303e-62
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = -3 
Query:  231 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 374
            KVLLSICSLLTDPNP+DPLVPEIAH+YK DK+KYESTA+ WTQKYAMG
Sbjct:  102 KVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 3.003e-62
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -1 
Query:  467 MASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 769
            MA KRI +EL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISK 101

HSP 2 Score: 80.1073 bits (196), Expect = 3.003e-62
Identity = 36/47 (76.60%), Postives = 40/47 (85.11%), Query Frame = -3 
Query:  234 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 374
            KVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:  101 KVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
The following BLAST results are available for this feature:
BLAST of CN186026 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 245
Match NameE-valueIdentityDescription
UB2D4_HUMAN7.309e-6479.21Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UBC1_COLGL7.309e-6478.22Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletot... [more]
UBC4_YEAST1.243e-6375.00Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UB2D2_XENLA1.039e-6279.21Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laev... [more]
UB2D2_RAT1.039e-6279.21Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norve... [more]
UB2D2_MOUSE1.039e-6279.21Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus... [more]
UB2D2_HUMAN1.039e-6279.21Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens... [more]
UB2D2_BOVIN1.039e-6279.21Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus G... [more]
UBC12_ARATH2.303e-6277.45Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UB2D3_RAT3.003e-6279.21Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002D24_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002D24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186026 ID=CN186026; Name=CN186026; organism=Citrus sinensis; type=EST; length=775bp
TTTTTTTTTTTTTTTTTACTTAAGACCATTAACTACGTTCCATCGAAGAA
GCTTCAAACACAAACATTCAACATTAACATAAAAACGTCCCGCCCTTGCA
ACTCCTCCCAACACCATTCTAAAACACCAACGTCTGCCCTCCCCAAAGCA
AGTTAAAGAAAATAGATAGACAACACACACAACATATTTCAATAAATGCT
CCTTTTTCTGCAATATCCTAACAACCTTCATCCCATGGCATACTTCTGTG
TCCAGCTCCTGGCCGTGGATTCATATTTGGCCTTGTCGGACTTGTACATG
TGGGCAATCTCCGGTACAAGAGGGTCGTCGGGATTTGGATCAGTAAGCAA
GGAGCAGATAGAGAGCAGGACTTTGCAAGATCCAAAGATGCACTTGGGAG
TACAACTTTATGATCCCAGGGTCAGTTTTACCTGGCTGTGCAAGACCAGA
GAGTTGTACGCCACTACTTTGAAATGGTAAGCGCTGGACTCCACTGTTCT
TTAAGGATATCAAGACAGATGCTTCCATTGCTGTTGATGTTCGGGTGGAA
GACCTTGGTCCTAAAAGCAACCTTTGGGGGCTTGAAAGGATAATCAGGAG
GAAAATGAATGGTAACTAAAAATACACCTCCTGCATATGGGCTATCAGAA
GGGCCCATAATTGTTGCTTGCCAGTGGAACATGTCTTCAGCCACAGGGCC
AGCGCTGCAAGACGTGGGAGGATCCTTCTGCAAATCCTTGAGTTCCCTTC
AAATACGCTTTGAAGCCATGACTCT
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