EG358208

Overview
NameEG358208
Unique NameEG358208
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length842
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEPK (Phosphoenolpyruvate carboxylase OS=Pseudomonas putida (strain KT2440) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.146e-33
Identity = 66/153 (43.14%), Postives = 92/153 (60.13%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++  ++ V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR +MD++A    + YRS+V + P FVEYFR +TPE E GR+ +GSRP+ R+
Sbjct:  548 AAAWAQYRAQENLVRICAEHQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPGIAEQNLNLYLAAVLEATLLPPPPPQPAWREVMDQLAADGVQAYRSVVRENPDFVEYFRQSTPEQELGRLPLGSRPAKRR 700

HSP 2 Score: 31.5722 bits (70), Expect = 6.146e-33
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            ++GGIES+RAIPWIF  T
Sbjct:  700 RAGGIESLRAIPWIFGWT 717
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEP1 (Phosphoenolpyruvate carboxylase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.146e-33
Identity = 66/153 (43.14%), Postives = 92/153 (60.13%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++  ++ V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR +MD++A    + YRS+V + P FVEYFR +TPE E GR+ +GSRP+ R+
Sbjct:  548 AAAWAQYRAQENLVRICAEHQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPGIAEQNLNLYLAAVLEATLLPPPPPQPAWREVMDQLAADGVQAYRSVVRENPDFVEYFRQSTPEQELGRLPLGSRPAKRR 700

HSP 2 Score: 31.5722 bits (70), Expect = 6.146e-33
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            ++GGIES+RAIPWIF  T
Sbjct:  700 RAGGIESLRAIPWIFGWT 717
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_KLEP3 (Phosphoenolpyruvate carboxylase OS=Klebsiella pneumoniae (strain 342) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 8.003e-33
Identity = 63/155 (40.65%), Postives = 95/155 (61.29%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  +++A LE  + PP  PK EWR +M E++ ++ K YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEVTISSLSLYTSAILEANLLPPPEPKAEWRDIMAELSDVSCKMYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 8.003e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSYIN (Phosphoenolpyruvate carboxylase OS=Psychromonas ingrahamii (strain 37) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 8.004e-33
Identity = 64/148 (43.24%), Postives = 92/148 (62.16%), Query Frame = 1 
Query:  226 YNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            Y +QE LVK++ +  + L +FH           P   A+LSQPP ++ G LRVT QGE+I   FG   +  ++L +++AA LE  + PP +PK EWR +M + +  + +EYR  V +EP FV YFR  TPE+E G++ +GSRPS RKP
Sbjct:  556 YTSQEALVKLSAENDINLVLFHGRGGTIGRGGAPARQALLSQPPGSLKGGLRVTEQGEMIRFKFGLPKVAVQSLNQYAAAVLEANLLPPPAPKQEWRDVMQQFSDQSCQEYRHFVREEPDFVPYFRSVTPEVELGKLALGSRPSKRKP 703

HSP 2 Score: 31.9574 bits (71), Expect = 8.004e-33
Identity = 13/14 (92.86%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIF 712
            SGGIES+RAIPWIF
Sbjct:  704 SGGIESLRAIPWIF 717
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEF5 (Phosphoenolpyruvate carboxylase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 8.004e-33
Identity = 67/153 (43.79%), Postives = 90/153 (58.82%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV + ++  V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR LMDE+A    + YR +V   P+FVEYFR +TPE E GR+ +GSRP+ R+
Sbjct:  549 AAAWAQYRAQERLVDICREQQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPPPQPAWRHLMDELASDGVRAYREVVRDNPQFVEYFRQSTPEQELGRLPLGSRPAKRR 701

HSP 2 Score: 31.5722 bits (70), Expect = 8.004e-33
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            ++GGIES+RAIPWIF  T
Sbjct:  701 RAGGIESLRAIPWIFGWT 718
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERPY (Phosphoenolpyruvate carboxylase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=ppc PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.357e-32
Identity = 64/155 (41.29%), Postives = 93/155 (60.00%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  ++ A LE  + PP  PK EW  +MD ++  +   YRS V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGITLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYAGAILEANLLPPPEPKKEWIEVMDLLSDASCDMYRSYVRENPEFVRYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.5722 bits (70), Expect = 1.357e-32
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  674 GGIESVRAIPWIFTCTHD 727
            GG+ES+RAIPWIF  T +
Sbjct:  706 GGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERPS (Phosphoenolpyruvate carboxylase OS=Yersinia pseudotuberculosis GN=ppc PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.357e-32
Identity = 64/155 (41.29%), Postives = 93/155 (60.00%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  ++ A LE  + PP  PK EW  +MD ++  +   YRS V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGITLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYAGAILEANLLPPPEPKKEWIEVMDLLSDASCDMYRSYVRENPEFVRYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.5722 bits (70), Expect = 1.357e-32
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  674 GGIESVRAIPWIFTCTHD 727
            GG+ES+RAIPWIF  T +
Sbjct:  706 GGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERPP (Phosphoenolpyruvate carboxylase OS=Yersinia pestis (strain Pestoides F) GN=ppc PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.357e-32
Identity = 64/155 (41.29%), Postives = 93/155 (60.00%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  ++ A LE  + PP  PK EW  +MD ++  +   YRS V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGITLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYAGAILEANLLPPPEPKKEWIEVMDLLSDASCDMYRSYVRENPEFVRYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.5722 bits (70), Expect = 1.357e-32
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  674 GGIESVRAIPWIFTCTHD 727
            GG+ES+RAIPWIF  T +
Sbjct:  706 GGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERPN (Phosphoenolpyruvate carboxylase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=ppc PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.357e-32
Identity = 64/155 (41.29%), Postives = 93/155 (60.00%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  ++ A LE  + PP  PK EW  +MD ++  +   YRS V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGITLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYAGAILEANLLPPPEPKKEWIEVMDLLSDASCDMYRSYVRENPEFVRYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.5722 bits (70), Expect = 1.357e-32
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  674 GGIESVRAIPWIFTCTHD 727
            GG+ES+RAIPWIF  T +
Sbjct:  706 GGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERPE (Phosphoenolpyruvate carboxylase OS=Yersinia pestis GN=ppc PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.357e-32
Identity = 64/155 (41.29%), Postives = 93/155 (60.00%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  ++ A LE  + PP  PK EW  +MD ++  +   YRS V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGITLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYAGAILEANLLPPPEPKKEWIEVMDLLSDASCDMYRSYVRENPEFVRYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.5722 bits (70), Expect = 1.357e-32
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  674 GGIESVRAIPWIFTCTHD 727
            GG+ES+RAIPWIF  T +
Sbjct:  706 GGVESLRAIPWIFAWTQN 723
The following BLAST results are available for this feature:
BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243
Match NameE-valueIdentityDescription
CAPP_PSEPK6.146e-3343.14Phosphoenolpyruvate carboxylase OS=Pseudomonas put... [more]
CAPP_PSEP16.146e-3343.14Phosphoenolpyruvate carboxylase OS=Pseudomonas put... [more]
CAPP_KLEP38.003e-3340.65Phosphoenolpyruvate carboxylase OS=Klebsiella pneu... [more]
CAPP_PSYIN8.004e-3343.24Phosphoenolpyruvate carboxylase OS=Psychromonas in... [more]
CAPP_PSEF58.004e-3343.79Phosphoenolpyruvate carboxylase OS=Pseudomonas flu... [more]
CAPP_YERPY1.357e-3241.29Phosphoenolpyruvate carboxylase OS=Yersinia pseudo... [more]
CAPP_YERPS1.357e-3241.29Phosphoenolpyruvate carboxylase OS=Yersinia pseudo... [more]
CAPP_YERPP1.357e-3241.29Phosphoenolpyruvate carboxylase OS=Yersinia pestis... [more]
CAPP_YERPN1.357e-3241.29Phosphoenolpyruvate carboxylase OS=Yersinia pestis... [more]
CAPP_YERPE1.357e-3241.29Phosphoenolpyruvate carboxylase OS=Yersinia pestis... [more]

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Properties
Property NameValue
Genbank description6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC
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