EG358208 | Citrus Genome Database

Overview


Name	EG358208
Unique Name	EG358208
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	842

Libraries


Library Name	Type
Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_NOCFA (Phosphoenolpyruvate carboxylase OS=Nocardia farcinica GN=ppc PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 3.416e-23
Identity = 65/161 (40.37%), Postives = 90/161 (55.90%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLE------HGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            L+A   LY A+  LV+VA++ G++L +FH            ++ AIL+QP   + G+LR+T QGEVI   + E     R L+   A TLE       G+     P  E   LMDE+A +A   Y  +V + P FVEYFR +TP  E G +NIGSRP++RKP
Sbjct:  590 LAANWALYRAELDLVEVARKTGIRLRLFHGRGGTVGRGGGRSYDAILAQPAGAVRGALRLTEQGEVIAAKYAEPGAAHRNLESLIAGTLESTLLDVEGLGADAEPAYE---LMDELAALARAAYARLVHETPGFVEYFRQSTPVAEVGDLNIGSRPASRKP 747

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURPS (Phosphoenolpyruvate carboxylase OS=Burkholderia pseudomallei GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURP6 (Phosphoenolpyruvate carboxylase OS=Burkholderia pseudomallei (strain 668) GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURMS (Phosphoenolpyruvate carboxylase OS=Burkholderia mallei (strain SAVP1) GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURMA (Phosphoenolpyruvate carboxylase OS=Burkholderia mallei GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURM9 (Phosphoenolpyruvate carboxylase OS=Burkholderia mallei (strain NCTC 10229) GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_BURM7 (Phosphoenolpyruvate carboxylase OS=Burkholderia mallei (strain NCTC 10247) GN=ppc PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.341e-23
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHP-PVSPK--PEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +LY A+  LV +  + G+KL +FH           PT+ AILSQPP T++G +R+T QGEVI   F    +  R L+   AATLE  + P   +PK  P + A M  ++  A   YR++V++ P F +YF  +TP  E   +NIGSRP++RK
Sbjct:  656 ELYRAELALVDLFDERGIKLRLFHGRGGTVGRGGGPTYQAILSQPPGTVNGQIRLTEQGEVIASKFANPEIGRRNLETVVAATLEATLAPHSNAPKQLPAFEAAMQTLSDAAMASYRALVYETPGFTDYFFSSTPITEIAELNIGSRPASRK 807

HSP 2 Score: 21.557 bits (44), Expect = 4.341e-23
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW F+
Sbjct:  816 IEDLRAIPWGFS 827

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_CORCT (Phosphoenolpyruvate carboxylase OS=Corynebacterium crenatum GN=ppc PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 7.358e-23
Identity = 58/155 (37.42%), Postives = 89/155 (57.42%), Query Frame = 1

Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRA--LMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            SA   LY+A+  LV++ +  GVKL +FH           P++ AIL+QP   + GS+R+T QGE+I   +G      R L+   +ATLE  +          RA  +M E++ ++ K+Y S+V ++  F++YF  +TP  E G +NIGSRPS+RK
Sbjct:  585 SANWALYDAELQLVELCRSAGVKLRLFHGRGGTVGRGGGPSYDAILAQPKGAVQGSVRITEQGEIISAKYGNPETARRNLEALVSATLEASLLDVSELTDHQRAYDIMSEISELSLKKYTSLVHEDQGFIDYFTQSTPLQEIGSLNIGSRPSSRK 739

HSP 2 Score: 24.6386 bits (52), Expect = 7.358e-23
Identity = 8/25 (32.00%), Postives = 15/25 (60.00%), Query Frame = 2

Query:  659 TESQSGGIESVRAIPWIFTCTHDDV 733
            +  Q+  +E +RAIPW+ + +   V
Sbjct:  737 SRKQTSSVEDLRAIPWVLSWSQSRV 761

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_CORGL (Phosphoenolpyruvate carboxylase OS=Corynebacterium glutamicum GN=ppc PE=1 SV=4)

HSP 1 Score: 103.605 bits (257), Expect = 9.573e-23
Identity = 58/155 (37.42%), Postives = 89/155 (57.42%), Query Frame = 1

Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRA--LMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            SA   LY+A+  LV++ +  GVKL +FH           P++ AIL+QP   + GS+R+T QGE+I   +G      R L+   +ATLE  +          RA  +M E++ ++ K+Y S+V ++  F++YF  +TP  E G +NIGSRPS+RK
Sbjct:  585 SANWALYDAELQLVELCRSAGVKLRLFHGRGGTVGRGGGPSYDAILAQPRGAVQGSVRITEQGEIISAKYGNPETARRNLEALVSATLEASLLDVSELTDHQRAYDIMSEISELSLKKYASLVHEDQGFIDYFTQSTPLQEIGSLNIGSRPSSRK 739

HSP 2 Score: 24.6386 bits (52), Expect = 9.573e-23
Identity = 8/25 (32.00%), Postives = 15/25 (60.00%), Query Frame = 2

Query:  659 TESQSGGIESVRAIPWIFTCTHDDV 733
            +  Q+  +E +RAIPW+ + +   V
Sbjct:  737 SRKQTSSVEDLRAIPWVLSWSQSRV 761

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ROSCS (Phosphoenolpyruvate carboxylase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) GN=ppc PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.107e-22
Identity = 50/148 (33.78%), Postives = 83/148 (56.08%), Query Frame = 1

Query:  223 LYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +Y AQ  L    +++G+++ +FH           P + AIL+QPP +I   +++T QGEVI   +G   L  R +++   A L  G+     P  EW   ++ +A ++ + YR++V++   FV YFR  TP  E  R+NIGSRP++R+
Sbjct:  602 IYQAQRKLRDFGRRHGIQMRLFHGRGGAIGRGGGPANHAILAQPPGSIGNQIKITEQGEVIADRYGLPLLAHRHIEQVINAVLRAGLLQRDDPPDEWTQALERLAALSQRHYRALVYERSDFVPYFRNVTPIAEISRLNIGSRPASRR 749

HSP 2 Score: 24.2534 bits (51), Expect = 2.107e-22
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2

Query:  680 IESVRAIPWIFT 715
            IE +RAIPW+F+
Sbjct:  754 IEDLRAIPWVFS 765

The following BLAST results are available for this feature:

BLAST of EG358208 vs. ExPASy Swiss-Prot

BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243


Match Name	E-value	Identity	Description
CAPP_NOCFA	3.416e-23	40.37	Phosphoenolpyruvate carboxylase OS=Nocardia farcin...	[more]
CAPP_BURPS	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ps...	[more]
CAPP_BURP6	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ps...	[more]
CAPP_BURMS	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ma...	[more]
CAPP_BURMA	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ma...	[more]
CAPP_BURM9	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ma...	[more]
CAPP_BURM7	4.341e-23	40.13	Phosphoenolpyruvate carboxylase OS=Burkholderia ma...	[more]
CAPP_CORCT	7.358e-23	37.42	Phosphoenolpyruvate carboxylase OS=Corynebacterium...	[more]
CAPP_CORGL	9.573e-23	37.42	Phosphoenolpyruvate carboxylase OS=Corynebacterium...	[more]
CAPP_ROSCS	2.107e-22	33.78	Phosphoenolpyruvate carboxylase OS=Roseiflexus cas...	[more]

Pages

Properties


Property Name	Value
Genbank description	6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC