EG358208

Overview
NameEG358208
Unique NameEG358208
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length842
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PROM5 (Phosphoenolpyruvate carboxylase OS=Prochlorococcus marinus (strain MIT 9515) GN=ppc PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.951e-16
Identity = 44/155 (28.39%), Postives = 79/155 (50.97%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGM-HPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            LS+  +++ AQ  L  ++ +  + L +FH           P + AIL+QP  T+ G +++T QGEV+   +    L    L+  + A +++ +    +   PEW  LM  +A  +  +YR +V + P  + +F+  TP  E  ++ I SRP+ RK
Sbjct:  635 LSSNWEIHRAQIALQNLSSKNNILLRLFHGRGGSVGRGGGPAYQAILAQPSGTLKGRIKITEQGEVLASKYSLPELALYNLETVTTAVIQNSLVINRLDATPEWNDLMTRLAETSRIQYRKLVHENPNLLTFFQEVTPIEEISKLQISSRPARRK 789

HSP 2 Score: 23.8682 bits (50), Expect = 3.951e-16
Identity = 11/25 (44.00%), Postives = 14/25 (56.00%), Query Frame = 2 
Query:  680 IESVRAIPWIFTCTHDDVPFTCVSW 754
            + S+RAIPW+F  T     F   SW
Sbjct:  795 LSSLRAIPWVFGWTQS--RFLLPSW 817
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_STRCO (Phosphoenolpyruvate carboxylase OS=Streptomyces coelicolor GN=ppc PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.958e-16
Identity = 55/153 (35.95%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLE-HGMHPPVSPKPE----WRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNR 663
            +++ AQ  L  VA +YGV+L +FH           PTH AIL+QP  T+ G ++VT QGEVI   +    L    L+   AATL+   +H       E    W A MD ++  A   YR +V ++P    YF  +TP  +   +++GSRPS R
Sbjct:  579 EIHRAQRRLRDVAHRYGVRLRLFHGRGGTVGRGGGPTHDAILAQPWGTLEGEIKVTEQGEVISDKYLIPALARENLELTVAATLQASALHTAPRQSDEALARWDAAMDVVSDAAHTAYRHLV-EDPDLPTYFLASTPVDQLADLHLGSRPSRR 730

HSP 2 Score: 22.3274 bits (46), Expect = 3.958e-16
Identity = 7/14 (50.00%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  680 IESVRAIPWIFTCT 721
            ++ +RAIPW+F  T
Sbjct:  737 LDGLRAIPWVFGWT 750
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_STRAW (Phosphoenolpyruvate carboxylase OS=Streptomyces avermitilis GN=ppc PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 5.144e-16
Identity = 54/153 (35.29%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLE-HGMHPPVSPKPE----WRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNR 663
            +++ AQ  L  VA +YGV+L +FH           P+H AIL+QP  T+ G ++VT QGEVI   +    L    L+   AATL+   +H       E    W A MD ++  A   YR +V ++P    YF  +TP  +   +++GSRPS R
Sbjct:  578 EIHRAQRRLRDVAHRYGVRLRLFHGRGGTVGRGGGPSHDAILAQPWGTLEGEIKVTEQGEVISDKYLVPSLARENLELTVAATLQASALHTAPRQSDEALARWDAAMDVVSDAAHSAYRRLV-EDPDLPTYFLASTPVDQLADLHLGSRPSRR 729

HSP 2 Score: 22.3274 bits (46), Expect = 5.144e-16
Identity = 7/14 (50.00%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  680 IESVRAIPWIFTCT 721
            ++ +RAIPW+F  T
Sbjct:  736 LDGLRAIPWVFGWT 749
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_CAUCR (Phosphoenolpyruvate carboxylase OS=Caulobacter crescentus GN=ppc PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.144e-16
Identity = 46/140 (32.86%), Postives = 71/140 (50.71%), Query Frame = 1 
Query:  244 LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNR 663
            L   A + GV L +FH           P   A+L+QP  T+ G +R+T QGE+I + FG++    R L   +AA L     P     P+    M  +A  + + YR++V+ +  F ++F   TP  E   +NIGSRP++R
Sbjct:  596 LAHEADRMGVGLQLFHGRGGSVGRGGGPAAEAVLAQPAGTVQGRIRMTEQGEMIARRFGDQPTARRNLDGLAAAVLMASERPIPKRDPKVEGAMTALAQSSFEGYRALVYDDAAFEDFFWSVTPISEIVGLNIGSRPASR 735

HSP 2 Score: 24.6386 bits (52), Expect = 5.144e-16
Identity = 9/19 (47.37%), Postives = 14/19 (73.68%), Query Frame = 2 
Query:  659 TESQSGGIESVRAIPWIFT 715
            + + S  IE +RAIPW+F+
Sbjct:  734 SRTASRKIEDLRAIPWVFS 752
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_THET8 (Phosphoenolpyruvate carboxylase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=ppc PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 9.677e-14
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1 
Query:  223 LYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKPKWR 678
            LY AQE L +V ++ G+ +  FH           P   AI S PP ++   +R+T QGE +   +    L  R L++         + P   P+  WR  + + A  +T+ YR+++ QE  F ++F   TP  E G + I SRP  R+ + R
Sbjct:  542 LYEAQEALSRVGEEVGLPVYFFHGRGTSTARGGGPAGRAIASLPPRSVGRRIRLTEQGEALADRYSHPDLAVRHLEQMLYHFALAALGPGQEPEARWREALAQAAEESTRRYRALL-QEEGFFDFFEAFTPIREIGELPIASRPVYRRGRVR 692

HSP 2 Score: 20.7866 bits (42), Expect = 9.677e-14
Identity = 7/14 (50.00%), Postives = 9/14 (64.29%), Query Frame = 2 
Query:  680 IESVRAIPWIFTCT 721
            I  +RAIPW+   T
Sbjct:  694 IRDLRAIPWVMAWT 707
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_THET2 (Phosphoenolpyruvate carboxylase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=ppc PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 9.677e-14
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1 
Query:  223 LYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKPKWR 678
            LY AQE L +V ++ G+ +  FH           P   AI S PP ++   +R+T QGE +   +    L  R L++         + P   P+  WR  + + A  +T+ YR+++ QE  F ++F   TP  E G + I SRP  R+ + R
Sbjct:  542 LYEAQEALSRVGEEVGLPVYFFHGRGTSTARGGGPAGRAIASLPPRSVGRRIRLTEQGEALADRYSHPDLAVRHLEQMLYHFALAALGPGQEPEARWREALAQAAEESTRRYRALL-QEEGFFDFFEAFTPIREIGELPIASRPVYRRGRVR 692

HSP 2 Score: 20.7866 bits (42), Expect = 9.677e-14
Identity = 7/14 (50.00%), Postives = 9/14 (64.29%), Query Frame = 2 
Query:  680 IESVRAIPWIFTCT 721
            I  +RAIPW+   T
Sbjct:  694 IRDLRAIPWVMAWT 707
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ARTAT (Phosphoenolpyruvate carboxylase OS=Arthrobacter aurescens (strain TC1) GN=ppc PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 2.116e-13
Identity = 46/153 (30.07%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLE-HGMH--PPVS--PKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNR 663
            +++  Q  L  VA ++GV++ +FH           PT+ AI++QP   + G ++ T QGEVI   +    L    L+   AA ++   +H  P  S   +  +  +M+ ++  A   YR+++  +P+   YF  +TP  + G +NIGSRPS R
Sbjct:  600 EIHKTQRKLRDVAAKHGVRVRLFHGRGGSVGRGGGPTYDAIMAQPNGVLEGEIKFTEQGEVISDKYSLPELARENLELSLAAVMQGSALHRTPRTSDDERERYANVMETISDAAFARYRTLI-DDPQLPAYFLASTPVEQLGSLNIGSRPSKR 751

HSP 2 Score: 23.0978 bits (48), Expect = 2.116e-13
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2 
Query:  677 GIESVRAIPWIFTCT 721
            G+  +RAIPW+F  T
Sbjct:  757 GLGGLRAIPWVFGWT 771
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: TRPD2_STRCO (Anthranilate phosphoribosyltransferase 2 OS=Streptomyces coelicolor GN=trpD2 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.591e-12
Identity = 38/53 (71.70%), Postives = 43/53 (81.13%), Query Frame = 1 
Query:    1 T*GDGANTVNISTGASILAAACGAKVAKQGSRSSSSACGIADVL*ALGVVIDL 159
            T GDG NT NIST A+I+AAACG +VAK G+RS+SSACG ADVL  LGV IDL
Sbjct:   71 TGGDGLNTFNISTAAAIVAAACGVRVAKHGNRSASSACGSADVLEELGVRIDL 123
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_METEA (Phosphoenolpyruvate carboxylase OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=ppc PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 2.223e-12
Identity = 43/151 (28.48%), Postives = 72/151 (47.68%), Query Frame = 1 
Query:  220 KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVS---PKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNR 663
            +LY AQ  L  +    GV +  FH           PTH  I +QPP +I G  R+T QGEV+   +         ++  +A+  EH +    +    + E+   ++ ++  +   Y +++ Q    V+YF+ A+P  E   +NIGSRP+ R
Sbjct:  591 ELYKAQVRLTTLGNHLGVPIAFFHGRGGSVSRGGVPTHRGIAAQPPGSIQGRFRITEQGEVVSFKYANRGTAAYQMELLAASVFEHALLSEGNGNGSRAEFDDALEALSGASRAAYVNLL-QAEGLVDYFQAASPLDEISLLNIGSRPARR 740

HSP 2 Score: 21.1718 bits (43), Expect = 2.223e-12
Identity = 6/11 (54.55%), Postives = 9/11 (81.82%), Query Frame = 2 
Query:  680 IESVRAIPWIF 712
            +  +RAIPW+F
Sbjct:  746 LSDLRAIPWVF 756
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: TRPD_THEFY (Anthranilate phosphoribosyltransferase OS=Thermobifida fusca (strain YX) GN=trpD PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.713e-12
Identity = 36/55 (65.45%), Postives = 46/55 (83.64%), Query Frame = 1 
Query:    1 T*GDGANTVNISTGASILAAACGAKVAKQGSRSSSSACGIADVL*ALGVVIDLDP 165
            T GD A+TVN+ST ASI+A+A GA+V K G+R++SS+CG ADVL  LGV+IDLDP
Sbjct:   84 TGGDRAHTVNVSTMASIVASAAGARVVKHGNRAASSSCGTADVLERLGVIIDLDP 138
The following BLAST results are available for this feature:
BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243
Match NameE-valueIdentityDescription
CAPP_PROM53.951e-1628.39Phosphoenolpyruvate carboxylase OS=Prochlorococcus... [more]
CAPP_STRCO3.958e-1635.95Phosphoenolpyruvate carboxylase OS=Streptomyces co... [more]
CAPP_STRAW5.144e-1635.29Phosphoenolpyruvate carboxylase OS=Streptomyces av... [more]
CAPP_CAUCR5.144e-1632.86Phosphoenolpyruvate carboxylase OS=Caulobacter cre... [more]
CAPP_THET89.677e-1430.26Phosphoenolpyruvate carboxylase OS=Thermus thermop... [more]
CAPP_THET29.677e-1430.26Phosphoenolpyruvate carboxylase OS=Thermus thermop... [more]
CAPP_ARTAT2.116e-1330.07Phosphoenolpyruvate carboxylase OS=Arthrobacter au... [more]
TRPD2_STRCO1.591e-1271.70Anthranilate phosphoribosyltransferase 2 OS=Strept... [more]
CAPP_METEA2.223e-1228.48Phosphoenolpyruvate carboxylase OS=Methylobacteriu... [more]
TRPD_THEFY2.713e-1265.45Anthranilate phosphoribosyltransferase OS=Thermobi... [more]

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Properties
Property NameValue
Genbank description6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC
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