EG358208

Overview
NameEG358208
Unique NameEG358208
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length842
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEE4 (Phosphoenolpyruvate carboxylase OS=Pseudomonas entomophila (strain L48) GN=ppc PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.361e-34
Identity = 68/153 (44.44%), Postives = 92/153 (60.13%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++ +++ V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WRALMD++A    K YR +V   P FVEYFR +TPE E GR+ +GSRP+ R+
Sbjct:  548 AAAWAQYRAQENLVRICREHQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVGGRFRTTEQGEMIRFKFGLPGIAEQNLNLYLAAVLEATLLPPPPPEPAWRALMDQLAADGVKAYRGVVRDNPEFVEYFRQSTPEQELGRLPLGSRPAKRR 700

HSP 2 Score: 31.5722 bits (70), Expect = 3.361e-34
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            ++GGIES+RAIPWIF  T
Sbjct:  700 RAGGIESLRAIPWIFGWT 717
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_VIBVY (Phosphoenolpyruvate carboxylase OS=Vibrio vulnificus (strain YJ016) GN=ppc PE=3 SV=2)

HSP 1 Score: 135.576 bits (340), Expect = 4.378e-34
Identity = 66/148 (44.59%), Postives = 93/148 (62.84%), Query Frame = 1 
Query:  226 YNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            Y+A E LV VA+Q G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I    G   +   +   +++A LE  + PP  PK EWR LM+ ++ ++ + YRS+V  EP FV YFR ATPE+E G++ +GSRP+ R P
Sbjct:  556 YHAMEALVNVAEQEGIELTLFHGRGGTIGRGGAPAHAALLSQPPKSLKGGLRVTEQGEMIRFKLGLPDVAVNSFNMYASAILEANLLPPPEPKQEWRDLMEVLSQVSCEAYRSVVRGEPDFVPYFRQATPELELGKLPLGSRPAKRNP 703

HSP 2 Score: 30.8018 bits (68), Expect = 4.378e-34
Identity = 11/15 (73.33%), Postives = 15/15 (100.00%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFT 715
            +GG+ES+RAIPWIF+
Sbjct:  704 NGGVESLRAIPWIFS 718
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_VIBVU (Phosphoenolpyruvate carboxylase OS=Vibrio vulnificus GN=ppc PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 4.378e-34
Identity = 66/148 (44.59%), Postives = 93/148 (62.84%), Query Frame = 1 
Query:  226 YNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            Y+A E LV VA+Q G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I    G   +   +   +++A LE  + PP  PK EWR LM+ ++ ++ + YRS+V  EP FV YFR ATPE+E G++ +GSRP+ R P
Sbjct:  556 YHAMEALVNVAEQEGIELTLFHGRGGTIGRGGAPAHAALLSQPPKSLKGGLRVTEQGEMIRFKLGLPDVAVNSFNMYASAILEANLLPPPEPKQEWRDLMEVLSQVSCEAYRSVVRGEPDFVPYFRQATPELELGKLPLGSRPAKRNP 703

HSP 2 Score: 30.8018 bits (68), Expect = 4.378e-34
Identity = 11/15 (73.33%), Postives = 15/15 (100.00%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFT 715
            +GG+ES+RAIPWIF+
Sbjct:  704 NGGVESLRAIPWIFS 718
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_MARMS (Phosphoenolpyruvate carboxylase OS=Marinomonas sp. (strain MWYL1) GN=ppc PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 5.702e-34
Identity = 64/147 (43.54%), Postives = 93/147 (63.27%), Query Frame = 1 
Query:  226 YNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            Y AQE L ++ K++G+ LT+FH           P H+AILSQPP +++G++RVT QGE+I   FG   +  R+L+ + +A +E  + P   PK EWRA+MDEMA +   +YRSI+     FV YFR  TPE E  ++ +GSRP+ R+
Sbjct:  556 YRAQEALTRLCKKHGIHLTLFHGRGGTVGRGGGPAHVAILSQPPGSVNGAIRVTEQGEMIRFKFGIPDIAVRSLELYCSAVMEASLIPAEPPKEEWRAIMDEMAEVGMNQYRSIIRGHEDFVPYFRATTPEQELAKLPLGSRPARRR 702

HSP 2 Score: 30.0314 bits (66), Expect = 5.702e-34
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 2 
Query:  674 GGIESVRAIPWIF 712
            GG+ES+RAIPWIF
Sbjct:  705 GGVESLRAIPWIF 717
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_VIBPA (Phosphoenolpyruvate carboxylase OS=Vibrio parahaemolyticus GN=ppc PE=3 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 5.702e-34
Identity = 68/155 (43.87%), Postives = 95/155 (61.29%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            +SA    Y+A E LVKVA+  GV+LT+FH           P H A+LSQPP ++ G LRVT QGE+I    G   +   +   +++A LE  + PP  PK EWR LM+ ++ ++ + YR +V  EP FV YFR ATPE+E G++ +GSRP+ R P
Sbjct:  549 MSAGWAQYHAMESLVKVAEDEGVELTLFHGRGGTVGRGGAPAHAALLSQPPKSLKGGLRVTEQGEMIRFKLGLPDVAVNSFNLYASAILEANLLPPPEPKQEWRDLMEVLSEVSCEAYRGVVRGEPDFVPYFRQATPELELGKLPLGSRPAKRNP 703

HSP 2 Score: 30.8018 bits (68), Expect = 5.702e-34
Identity = 11/15 (73.33%), Postives = 15/15 (100.00%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFT 715
            +GG+ES+RAIPWIF+
Sbjct:  704 NGGVESLRAIPWIFS 718
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEMY (Phosphoenolpyruvate carboxylase OS=Pseudomonas mendocina (strain ymp) GN=ppc PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 7.426e-34
Identity = 67/153 (43.79%), Postives = 93/153 (60.78%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++  ++ V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP +P+P WRA+MD +A +  K YR +V + P+FV YFR ATPE E GR+ +GSRP+ R+
Sbjct:  551 AAAWAQYRAQEELVRLCGEHQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPAPEPSWRAMMDRLADVGVKAYRGVVREHPQFVAYFRQATPEQELGRLPLGSRPAKRR 703

HSP 2 Score: 31.5722 bits (70), Expect = 7.426e-34
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            + GG+ES+RAIPWIF  T
Sbjct:  703 REGGVESLRAIPWIFAWT 720
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PHOLL (Phosphoenolpyruvate carboxylase OS=Photorhabdus luminescens subsp. laumondii GN=ppc PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 7.426e-34
Identity = 65/155 (41.94%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++AA   Y AQ+ L+ V ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  +++A LE  + PP  PKPEW  +MD ++ ++ K YR  V ++P FV YFR ATPE E  ++ +GSRP+ R P
Sbjct:  550 MAAAWAQYRAQDALINVCEKEGITLTLFHGRGGTIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPQVTISSLALYASAILEANLLPPPEPKPEWHQVMDTLSDVSCKMYRDYVREQPDFVPYFRAATPEQELAKLPLGSRPAKRHP 704

HSP 2 Score: 31.9574 bits (71), Expect = 7.426e-34
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  705 AGGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ACIBT (Phosphoenolpyruvate carboxylase OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 9.669e-34
Identity = 68/154 (44.16%), Postives = 94/154 (61.04%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +SA    Y AQE L  +A+++GV+LT+FH           PT  A+ SQPP +I G++RVT QGE+I   FG E +  + L+ ++AATLE  + PP  PK EWR LM+ M   + K YR  V + P FV+Y R  TPE+E   + +GSRP+ RK
Sbjct:  561 MSANWAQYRAQEELTAIARKHGVQLTLFHGRGGSISRGGAPTQQALFSQPPGSISGAIRVTEQGEMIRFKFGLEGIAMQNLEIYTAATLEATLLPPPEPKAEWRELMNRMTDHSVKVYRQTVRENPHFVKYLRTVTPELELQMLPLGSRPAKRK 714

HSP 2 Score: 32.7278 bits (73), Expect = 9.669e-34
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCT 721
            SGGIES+RAIPW+F  T
Sbjct:  716 SGGIESLRAIPWVFAWT 732
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_SALPK (Phosphoenolpyruvate carboxylase OS=Salmonella paratyphi A (strain AKU_12601) GN=ppc PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.671e-34
Identity = 64/155 (41.29%), Postives = 96/155 (61.94%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  +++A LE  + PP  PK  WR +MDE++VI+ + YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLEGGLRVTEQGEMIRFKYGLPEVTVSSLSLYTSAILEANLLPPPEPKDSWRHIMDELSVISCETYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 9.671e-34
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_SALPA (Phosphoenolpyruvate carboxylase OS=Salmonella paratyphi A GN=ppc PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.671e-34
Identity = 64/155 (41.29%), Postives = 96/155 (61.94%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  +++A LE  + PP  PK  WR +MDE++VI+ + YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLEGGLRVTEQGEMIRFKYGLPEVTVSSLSLYTSAILEANLLPPPEPKDSWRHIMDELSVISCETYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 9.671e-34
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
The following BLAST results are available for this feature:
BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243
Match NameE-valueIdentityDescription
CAPP_PSEE43.361e-3444.44Phosphoenolpyruvate carboxylase OS=Pseudomonas ent... [more]
CAPP_VIBVY4.378e-3444.59Phosphoenolpyruvate carboxylase OS=Vibrio vulnific... [more]
CAPP_VIBVU4.378e-3444.59Phosphoenolpyruvate carboxylase OS=Vibrio vulnific... [more]
CAPP_MARMS5.702e-3443.54Phosphoenolpyruvate carboxylase OS=Marinomonas sp.... [more]
CAPP_VIBPA5.702e-3443.87Phosphoenolpyruvate carboxylase OS=Vibrio parahaem... [more]
CAPP_PSEMY7.426e-3443.79Phosphoenolpyruvate carboxylase OS=Pseudomonas men... [more]
CAPP_PHOLL7.426e-3441.94Phosphoenolpyruvate carboxylase OS=Photorhabdus lu... [more]
CAPP_ACIBT9.669e-3444.16Phosphoenolpyruvate carboxylase OS=Acinetobacter b... [more]
CAPP_SALPK9.671e-3441.29Phosphoenolpyruvate carboxylase OS=Salmonella para... [more]
CAPP_SALPA9.671e-3441.29Phosphoenolpyruvate carboxylase OS=Salmonella para... [more]

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Properties
Property NameValue
Genbank description6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC
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