EG358208 | Citrus Genome Database

Overview


Name	EG358208
Unique Name	EG358208
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	842

Libraries


Library Name	Type
Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOK1 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O1:K1 / APEC GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.136e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.136e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECODH (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain K12 / DH10B) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.136e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.136e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOBW (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain K12 / BW2952) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.136e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.136e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO45 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.136e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.136e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO27 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.136e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.136e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSESM (Phosphoenolpyruvate carboxylase OS=Pseudomonas syringae pv. tomato GN=ppc PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.136e-33
Identity = 67/153 (43.79%), Postives = 91/153 (59.48%), Query Frame = 1

Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++ ++  V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR +MD+MA      YR++V + P FVEYFR ATPE E GR+ +GSRP+ R+
Sbjct:  551 AAAWAQYRAQEKLVEICREQQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPPPQPAWRTMMDQMASDGVSAYRAVVRENPEFVEYFRQATPEQELGRLPLGSRPAKRR 703

HSP 2 Score: 31.5722 bits (70), Expect = 2.136e-33
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  668 QSGGIESVRAIPWIFTCT 721
            + GG+ES+RAIPWIF  T
Sbjct:  703 REGGVESLRAIPWIFAWT 720

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_SHISS (Phosphoenolpyruvate carboxylase OS=Shigella sonnei (strain Ss046) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_SHIFL (Phosphoenolpyruvate carboxylase OS=Shigella flexneri GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_SHIF8 (Phosphoenolpyruvate carboxylase OS=Shigella flexneri serotype 5b (strain 8401) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOSM (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

The following BLAST results are available for this feature:

BLAST of EG358208 vs. ExPASy Swiss-Prot

BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243


Match Name	E-value	Identity	Description
CAPP_ECOK1	2.136e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECODH	2.136e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOBW	2.136e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO45	2.136e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO27	2.136e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_PSESM	2.136e-33	43.79	Phosphoenolpyruvate carboxylase OS=Pseudomonas syr...	[more]
CAPP_SHISS	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Shigella sonnei...	[more]
CAPP_SHIFL	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Shigella flexne...	[more]
CAPP_SHIF8	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Shigella flexne...	[more]
CAPP_ECOSM	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]

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Properties


Property Name	Value
Genbank description	6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC