EG358208 | Citrus Genome Database

Overview


Name	EG358208
Unique Name	EG358208
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	842

Libraries


Library Name	Type
Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOSE (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain SE11) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOLU (Phosphoenolpyruvate carboxylase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOLC (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOL6 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O6 GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOL5 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECOHS (Phosphoenolpyruvate carboxylase OS=Escherichia coli O9:H4 (strain HS) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO8A (Phosphoenolpyruvate carboxylase OS=Escherichia coli O8 (strain IAI1) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO7I (Phosphoenolpyruvate carboxylase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO5E (Phosphoenolpyruvate carboxylase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO57 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O157:H7 GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1

Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2

Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724

The following BLAST results are available for this feature:

BLAST of EG358208 vs. ExPASy Swiss-Prot

BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243


Match Name	E-value	Identity	Description
CAPP_ECOSE	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOLU	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOLC	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOL6	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOL5	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECOHS	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO8A	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO7I	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO5E	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]
CAPP_ECO57	2.782e-33	41.29	Phosphoenolpyruvate carboxylase OS=Escherichia col...	[more]

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Properties


Property Name	Value
Genbank description	6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC