EG358208

Overview
NameEG358208
Unique NameEG358208
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length842
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO55 (Phosphoenolpyruvate carboxylase OS=Escherichia coli (strain 55989 / EAEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ECO24 (Phosphoenolpyruvate carboxylase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEU5 (Phosphoenolpyruvate carboxylase OS=Pseudomonas stutzeri (strain A1501) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.782e-33
Identity = 67/153 (43.79%), Postives = 91/153 (59.48%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV+V +++GV+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR  MD +A      YR +V + P FV+YFR ATPE+E GR+ +GSRP+ R+
Sbjct:  552 AAAWAQYRAQEQLVEVCREHGVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPPPEPGWRETMDRLAAEGVAAYRGVVREHPLFVDYFRQATPELELGRLPLGSRPAKRR 704

HSP 2 Score: 31.5722 bits (70), Expect = 2.782e-33
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            + GG+ES+RAIPWIF  T
Sbjct:  704 REGGVESLRAIPWIFAWT 721
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PSEU2 (Phosphoenolpyruvate carboxylase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.782e-33
Identity = 67/153 (43.79%), Postives = 91/153 (59.48%), Query Frame = 1 
Query:  208 SAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            +AA   Y AQE LV++ ++  V+L +FH           P H AILSQPP ++ G  R T QGE+I   FG   +  + L  + AA LE  + PP  P+P WR +MD+MA      YR++V + P FVEYFR ATPE E GR+ +GSRP+ R+
Sbjct:  551 AAAWAQYRAQERLVEICREQQVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPPPQPAWRTMMDQMADDGVSAYRAVVRENPEFVEYFRQATPEQELGRLPLGSRPAKRR 703

HSP 2 Score: 31.5722 bits (70), Expect = 2.782e-33
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2 
Query:  668 QSGGIESVRAIPWIFTCT 721
            + GG+ES+RAIPWIF  T
Sbjct:  703 REGGVESLRAIPWIFAWT 720
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_PECCP (Phosphoenolpyruvate carboxylase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.782e-33
Identity = 63/155 (40.65%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK EW  +MDE++ ++   YR  V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYRAQDALIKTCEKAGIALTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEVTISSLALYTGAILEANLLPPPEPKQEWHEVMDELSRVSCDMYRGYVRENPDFVPYFRAATPELELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 2.782e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 NGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_YERE8 (Phosphoenolpyruvate carboxylase OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=ppc PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.624e-33
Identity = 63/155 (40.65%), Postives = 95/155 (61.29%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   FG   +   +L  +++A LE  + PP  PK EW  +MD ++  + + YR  V + P+FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  550 MAASWAQYRAQDALIKTCEKAGISLTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKFGLPEVTISSLALYASAVLEANLLPPPEPKKEWNEVMDILSDASCEMYRGYVRENPQFVPYFRAATPELELGKLPLGSRPAKRRP 704

HSP 2 Score: 31.9574 bits (71), Expect = 3.624e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  705 NGGVESLRAIPWIFAWTQN 723
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ERWCT (Phosphoenolpyruvate carboxylase OS=Erwinia carotovora subsp. atroseptica GN=ppc PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.624e-33
Identity = 63/155 (40.65%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G+ LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK EW  +MDE++ ++   YR  V + P FV YFR ATPE+E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYRAQDALIKTCEKAGIALTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPDVTISSLALYTGAILEANLLPPPEPKQEWHEVMDELSRVSCDMYRGYVRENPDFVPYFRAATPELELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 3.624e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 NGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_CITK8 (Phosphoenolpyruvate carboxylase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 4.719e-33
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            ++A+   Y AQ+ L+K  ++ G++LT+FH           P H A+LSQPP ++ G LRVT QGE+I   +G   +   +L  ++ A LE  + PP  PK  WR +MDE++VI+   YR  V +   FV YFR ATPE E G++ +GSRP+ R+P
Sbjct:  551 MAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEVTISSLSLYTGAILEANLLPPPEPKNSWRHIMDELSVISCDLYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRP 705

HSP 2 Score: 31.9574 bits (71), Expect = 4.719e-33
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCTHD 727
            +GG+ES+RAIPWIF  T +
Sbjct:  706 TGGVESLRAIPWIFAWTQN 724
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_ACTP2 (Phosphoenolpyruvate carboxylase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=ppc PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 4.719e-33
Identity = 68/155 (43.87%), Postives = 94/155 (60.65%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRKP 669
            L+A+   Y AQE LV +A++Y ++L +FH           P H A+LSQPP ++   LRVT QGE+I    G   +   TL  +++A LE  + PP  PK +WR +MD+ A I+ + YR +V  EP FV YFR ATPE E G++ +GSRPS R P
Sbjct:  550 LAASWAQYRAQEALVNLAEKYHIELVLFHGRGGTVGRGGAPAHAALLSQPPRSLKSGLRVTEQGEMIRFKLGLPAVAVETLNLYASAILEANLLPPPEPKQKWRDIMDKGAAISCEIYRGVVRGEPDFVPYFRSATPEQELGKLPLGSRPSKRNP 704

HSP 2 Score: 30.4166 bits (67), Expect = 4.719e-33
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIF 712
            +GG+ES+RAIPWIF
Sbjct:  705 NGGVESLRAIPWIF 718
[Close]
BLAST of EG358208 vs. ExPASy Swiss-Prot
Match: CAPP_MARAV (Phosphoenolpyruvate carboxylase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=ppc PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.145e-33
Identity = 67/154 (43.51%), Postives = 92/154 (59.74%), Query Frame = 1 
Query:  205 LSAA*KLYNAQE*LVKVAKQYGVKLTMFHXXXXXXXXXXXPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFSAATLEHGMHPPVSPKPEWRALMDEMAVIATKEYRSIVFQEPRFVEYFRLATPEMEYGRMNIGSRPSNRK 666
            L AA   Y AQE L +VA +YGV LT+FH           P + AILSQPP +++GS R+T QGE+I   FG   L  ++L  ++ A +E  + PP  PK EWR +MD +   + + YR +V + P FV YFR  TPE   G++ +GSRP+ RK
Sbjct:  549 LMAAWAQYQAQEKLTEVANRYGVHLTLFHGRGGTVGRGGGPANRAILSQPPGSVNGSFRITEQGEMIRFKFGLPRLAVQSLTLYTTAVIEATLAPPPVPKDEWREVMDWLTERSLRSYREVVRENPDFVPYFRQVTPETALGKLALGSRPARRK 702

HSP 2 Score: 31.5722 bits (70), Expect = 6.145e-33
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  671 SGGIESVRAIPWIFTCT 721
            +GG+ES+RAIPWIF  T
Sbjct:  704 TGGVESLRAIPWIFAWT 720
The following BLAST results are available for this feature:
BLAST of EG358208 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 243
Match NameE-valueIdentityDescription
CAPP_ECO552.782e-3341.29Phosphoenolpyruvate carboxylase OS=Escherichia col... [more]
CAPP_ECO242.782e-3341.29Phosphoenolpyruvate carboxylase OS=Escherichia col... [more]
CAPP_PSEU52.782e-3343.79Phosphoenolpyruvate carboxylase OS=Pseudomonas stu... [more]
CAPP_PSEU22.782e-3343.79Phosphoenolpyruvate carboxylase OS=Pseudomonas syr... [more]
CAPP_PECCP2.782e-3340.65Phosphoenolpyruvate carboxylase OS=Pectobacterium ... [more]
CAPP_YERE83.624e-3340.65Phosphoenolpyruvate carboxylase OS=Yersinia entero... [more]
CAPP_ERWCT3.624e-3340.65Phosphoenolpyruvate carboxylase OS=Erwinia carotov... [more]
CAPP_CITK84.719e-3341.29Phosphoenolpyruvate carboxylase OS=Citrobacter kos... [more]
CAPP_ACTP24.719e-3343.87Phosphoenolpyruvate carboxylase OS=Actinobacillus ... [more]
CAPP_MARAV6.145e-3343.51Phosphoenolpyruvate carboxylase OS=Marinobacter aq... [more]

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Properties
Property NameValue
Genbank description6b11 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl006b11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358208 ID=EG358208; Name=EG358208; organism=Citrus sinensis; type=EST; length=842bp
ACCTGAGGTGATGGTGCAAACACAGTGAACATTTCAACTGGTGCTTCAAT
TCTTGCTGCAGCTTGTGGTGCAAAAGTTGCCAAGCAAGGGAGTCGGTCTA
GTTCTTCTGCATGTGGAATTGCTGATGTGTTATAAGCTTTGGGTGTCGTT
ATTGACTTGGACCCTATGGGTGGGAGAAGGGGTGTGGGAGAGGATGCTGG
TGGTTTGTCGGCGGCTTGAAAGTTATATAATGCTCAGGAATAGCTTGTGA
AGGTCGCAAAGCAGTATGGTGTTAAACTCACAATGTTCCATGGCCGAGGA
GGGACAGTCGGAAGAGGTGGAGGACCCACCCATCTGGCTATATTGTCTCA
ACCACCTGATACAATTCATGGTTCACTTCGTGTGACGGTTCAAGGAGAAG
TTATTGAGCAGTCTTTTGGGGAGGAGCACTTGTGCTTCAGAACACTTCAG
CGTTTCTCAGCTGCTACACTTGAGCATGGAATGCATCCCCCAGTCTCACC
AAAACCAGAATGGCGTGCACTCATGGATGAGATGGCTGTCATTGCTACAA
AGGAATATCGTTCTATAGTATTCCAGGAACCTCGTTTTGTCGAATATTTT
CGCCTTGCAACACCAGAAATGGAGTATGGTCGGATGAACATTGGAAGTCG
TCCATCAAACCGAAAGCCAAAGTGGAGGTATTGAGTCAGTCCGTGCCATC
CCATGGATCTTCACATGCACTCACGACGATGTTCCATTTACCTGTGTGTC
TTGGTCTTTGTGGGCACATTTAAACATGTCATTCAGAAGGATATAAAGAA
TCTTGCACATGCCTACAGGAGATGAACTTGGCCGCTACACTC
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