CN185908

Overview
NameCN185908
Unique NameCN185908
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length771
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_CLOTH (Ketol-acid reductoisomerase OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.234e-13
Identity = 49/148 (33.11%), Postives = 78/148 (52.70%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAYKNTVECI--TGIISKIISTQGMLAVYNSFSGEDKKEF 560
            P  + VI + PKG G +VR  YV+GK     G+ S  AV+QD  G+A + AL ++  +G+       TT ++E  +D+FGE+ +L G V  ++++ F    E G   ++AY    ECI    +I  +I+  G    Y  +S  D  E+
Sbjct:  120 PPEVDVIMIAPKGPGHTVRSQYVEGK-----GVPSLIAVYQDASGKAKDYALAYAAGIGAGRAGILETTFKEETETDLFGEQAVLCGGVTELMKAGFETLVEAGYQPEIAY---FECIHEMKLIVDLINQGGF--SYMRYSISDTAEY 257
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_THEP1 (Ketol-acid reductoisomerase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 60/224 (26.79%), Postives = 107/224 (47.77%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALG--SPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAYKNTVECIT--GIISKIISTQGMLAVYNSFSGEDKKEFEKAYSASYYPCMEI---LYECYEDVAAGSEIRSVVL---AGRRFYEKEGLPAFPMGKIDQTRM-WKVGERVRSTRP 767
            PKN+ V  + PK  G  VRR YV+G+     G+ +  AV+QD  G+A ++AL ++  +G        TT ++E  +D+FGE+ +L G V  ++++ F    E G   ++AY    EC+    +I  +I   G+   +  +S  +  E+    S       E+   + +  +D+  G   +  +L   AGR ++       + M K +   +  KVG+ +R   P
Sbjct:  120 PKNVDVTMIAPKSPGHIVRREYVEGR-----GVPALVAVYQDYTGKAKDIALAYAKGIGVTRAGVIETTFKEETETDLFGEQAVLCGGVTALIKAGFETLVEAGYQPEIAY---FECLNELKLIVDLIYEGGL--SFMRYSVSNTAEYGDYISQEKIVTKEVRENMKQMLKDIQTGKFAKDWILENQAGRPYF-------YTMRKKESEHLIEKVGKELRKMMP 326
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAW (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain MW2) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAS (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain MSSA476) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAR (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain MRSA252) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAN (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain N315) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAM (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAAB (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAA9 (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain JH9) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
[Close]
BLAST of CN185908 vs. ExPASy Swiss-Prot
Match: ILVC_STAA2 (Ketol-acid reductoisomerase OS=Staphylococcus aureus (strain JH1) GN=ilvC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.142e-13
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  129 PKNIGVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATNVALGWSVALGSPFT--FATTLEQEYRSDIFGERGILLGAVHGIVESLFRRFTENGMNEDLAY 455
            P ++ V  V PKG G  VRR +V+G     + + S F + QD  G+A N+AL ++  +G+       TT ++E  +D+FGE+ +L G V  +++S F    E G   +LAY
Sbjct:  119 PADVDVFLVAPKGPGHLVRRTFVEG-----SAVPSLFGIQQDASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAY 224
The following BLAST results are available for this feature:
BLAST of CN185908 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 213
Match NameE-valueIdentityDescription
ILVC_CLOTH6.234e-1333.11Ketol-acid reductoisomerase OS=Clostridium thermoc... [more]
ILVC_THEP18.142e-1326.79Ketol-acid reductoisomerase OS=Thermotoga petrophi... [more]
ILVC_STAAW8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAAS8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAAR8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAAN8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAAM8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAAB8.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAA98.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]
ILVC_STAA28.142e-1335.14Ketol-acid reductoisomerase OS=Staphylococcus aure... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002A19_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002A19, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185908 ID=CN185908; Name=CN185908; organism=Citrus sinensis; type=EST; length=771bp
GCGGTATTTCTGATGCTGCTCAGGCGGATAATTATGAGAAGATATTCTCT
TGCATGAAACCAAACAGCATCCTTGGACTTTCCCATGGGTTCCTTCTTGG
GCATTTGCAGTCAATGGGACTTGATTTCCCAAAGAATATCGGTGTGATTG
CTGTGTGTCCCAAGGGAATGGGTCCATCTGTGAGGAGACTCTATGTTCAA
GGCAAAGAGATTAATGGTGCTGGAATTAATTCCAGTTTTGCTGTCCACCA
GGATGTTGATGGAAGAGCTACCAATGTCGCCCTAGGATGGTCAGTTGCCC
TTGGTTCTCCTTTCACATTTGCCACCACATTAGAGCAGGAGTACAGGAGT
GACATCTTTGGGGAGCGTGGCATTCTACTTGGTGCTGTTCATGGAATTGT
GGAATCCTTGTTTAGAAGGTTCACTGAAAATGGAATGAATGAGGATCTGG
CTTACAAGAATACCGTGGAGTGCATAACAGGAATTATATCAAAGATCATC
TCAACTCAGGGTATGTTGGCTGTCTACAATTCCTTCTCTGGAGAAGACAA
AAAGGAATTCGAGAAAGCTTATAGTGCCTCATATTATCCGTGCATGGAAA
TCTTATATGAGTGCTATGAAGATGTGGCCGCTGGCAGTGAGATCCGCAGT
GTTGTCTTGGCTGGACGTCGGTTTTATGAAAAGGAAGGTCTACCAGCTTT
CCCTATGGGAAAAATTGATCAGACTCGCATGTGGAAAGTTGGTGAGCGAG
TCCGATCAACACGTCCAGCAG
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