CN185800

Overview
NameCN185800
Unique NameCN185800
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length623
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
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BLAST of CN185800 vs. ExPASy Swiss-Prot
Match: THI4_UROFA (Thiazole biosynthetic enzyme, mitochondrial OS=Uromyces fabae GN=THI2 PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 6.668e-35
Identity = 76/149 (51.01%), Postives = 102/149 (68.46%), Query Frame = 2 
Query:  188 LQPIKSSRPNNLSISASA---SPPYDLNTFKFDPIKESIVSREMTLRYMTDMITYADTDXXXXGAGSAGLSCAYEL-SKNPNIQIAITEQSVSPGGGAWLVGQLFSAMVVRKPAHIFLDELGINYDEQDNYVVIKHAALFTSTIMSKLL 622
            + P+KS + +N +++  A    P  +L   KF PIKE  V R M  RY  DM   A +D    GAGSAGLSCAY L +  P+++I I E +V+PGGG WL GQL SAMV RKPA  FLD++G+ Y+++ N+VV+KHAALFTST++SK+L
Sbjct:   24 MDPLKSKQQSNATVNKPAFKPEPAVNLTPIKFAPIKEHQVQRAMVRRYFQDMEERAISDVIIVGAGSAGLSCAYALGTARPDLKITILESNVAPGGGCWLGGQLMSAMVCRKPADEFLDQVGVPYEDEGNFVVVKHAALFTSTVLSKVL 172
[Close]
BLAST of CN185800 vs. ExPASy Swiss-Prot
Match: THI4_THEMA (Putative thiazole biosynthetic enzyme OS=Thermotoga maritima GN=TM_0787 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.353e-11
Identity = 33/99 (33.33%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  281 IKESIVSREMTLRYMTDMITYADTDXXXXGAGSAGLSCAYELSKNPNIQIAITEQSVSPGGGAWLVGQLFSAMVVRKPAHIFLDELGINYDEQDNYVVI 577
            +++ ++SR +  RY   +    + D    GAG +GL+ AYEL+KN   ++A+ E+  +PGGG W  G +F+ +V+ K    FL E+ I Y+ +++++V+
Sbjct:    3 MRDVLISRLIVERYFEKLRNSLELDVAIVGAGPSGLTAAYELAKN-GFRVAVFEERNTPGGGIWGGGMMFNEIVLEKELENFLKEVEIEYEVKEDHIVV 100
[Close]
BLAST of CN185800 vs. ExPASy Swiss-Prot
Match: THI4_PYRHO (Putative thiazole biosynthetic enzyme OS=Pyrococcus horikoshii GN=PH1357 PE=3 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 6.715e-11
Identity = 38/114 (33.33%), Postives = 62/114 (54.39%), Query Frame = 2 
Query:  281 IKESIVSREMTLRYMTDMITYADTDXXXXGAGSAGLSCAYELSKNPNIQIAITEQSVSPGGGAWLVGQLFSAMVVRKPAHIFLDELGINYDEQDNYVVIKHAALFTSTIMSKLL 622
            ++E  ++R +   Y  D++   + D    GAG +G+  AY L+K    ++AI E+ +S GGG W  G  F+ +VV+  A   LDE GI Y+E +    +  A    +TI SK++
Sbjct:    2 LREVTITRAIVESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIGGGIWGGGMGFNKVVVQDEAREILDEFGIRYEEFEKGYYVADAIEVATTIASKVV 114
The following BLAST results are available for this feature:
BLAST of CN185800 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
THI4_UROFA6.668e-3551.01Thiazole biosynthetic enzyme, mitochondrial OS=Uro... [more]
THI4_THEMA1.353e-1133.33Putative thiazole biosynthetic enzyme OS=Thermotog... [more]
THI4_PYRHO6.715e-1133.33Putative thiazole biosynthetic enzyme OS=Pyrococcu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001O05_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001O05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185800 ID=CN185800; Name=CN185800; organism=Citrus sinensis; type=EST; length=623bp
ATTACTGCTGCAAATCAAATTACTTCTCATTATCAAACTCCTTAGCAGCA
GGGGAAGGAGGGGTAAAAAAAAAAAACCATGGCAGCCATGGCTTCTACAG
CTTTCGCTCCTTCAGTTTCATCCACCACCAATAAGCTCTTTGACTCATCC
TTTCATGGGGCTCCCATGTCACCATTCTCTTCTACGTCTTCAACCCATCA
AATCTTCTCGTCCCAACAACTTGTCCATTTCTGCTTCTGCTTCTCCTCCT
TATGACTTGAACACTTTCAAGTTTGACCCCATCAAAGAATCCATTGTTTC
TCGTGAAATGACACTCAGGTACATGACGGACATGATCACTTATGCTGACA
CTGATGTGGTAGTGGTTGGTGCTGGCTCTGCTGGCCTCTCTTGTGCTTAT
GAGCTCAGCAAAAACCCCAACATTCAGATTGCTATTACCGAACAGTCTGT
TAGCCCCGGCGGCGGCGCGTGGCTCGTTGGACAGCTCTTCTCTGCCATGG
TTGTGCGCAAACCAGCCCACATCTTCCTTGACGAGCTTGGCATCAACTAC
GATGAGCAAGACAACTATGTTGTGATCAAGCATGCAGCGCTGTTCACCTC
CACTATAATGAGCAAGCTCCTGG
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