CN182135 | Citrus Genome Database

Overview


Name	CN182135
Unique Name	CN182135
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	596

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A_SPOFR (Eukaryotic translation initiation factor 5A OS=Spodoptera frugiperda GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.006e-21
Identity = 40/63 (63.49%), Postives = 53/63 (84.13%), Query Frame = -1

Query:  408 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPN 596
            K H VGIDIFNGKK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ +NG+ ++DL++P+
Sbjct:   57 KVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPD 119

HSP 2 Score: 30.0314 bits (66), Expect = 1.006e-21
Identity = 14/35 (40.00%), Postives = 25/35 (71.43%), Query Frame = -2

Query:  302 DENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 406
            D +L +Q++  F+SGK+L+ +V  + GEE + A+K
Sbjct:  119 DGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVK 153

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A_SPOEX (Eukaryotic translation initiation factor 5A OS=Spodoptera exigua GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.006e-21
Identity = 40/63 (63.49%), Postives = 53/63 (84.13%), Query Frame = -1

Query:  408 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPN 596
            K H VGIDIFNGKK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ +NG+ ++DL++P+
Sbjct:   57 KVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPD 119

HSP 2 Score: 30.0314 bits (66), Expect = 1.006e-21
Identity = 14/35 (40.00%), Postives = 25/35 (71.43%), Query Frame = -2

Query:  302 DENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 406
            D +L +Q++  F+SGK+L+ +V  + GEE + A+K
Sbjct:  119 DGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVK 153

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A2_SCHPO (Eukaryotic translation initiation factor 5A-2 OS=Schizosaccharomyces pombe GN=tif51b PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.147e-19
Identity = 36/62 (58.06%), Postives = 50/62 (80.65%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H V +DIFNG+K ED+ PS+HN DVP V R +YQL++I +DG+++L+T +G TKDD+RLP
Sbjct:   57 KVHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLP 117

HSP 2 Score: 37.3502 bits (85), Expect = 1.147e-19
Identity = 16/31 (51.61%), Postives = 24/31 (77.42%), Query Frame = -2

Query:  299 SQIKDGFESGKDLVVSVQCAMGEEQINALKD 391
            ++I++GFE GKDL+++V  AMGEE   A +D
Sbjct:  123 NEIEEGFEEGKDLIITVVSAMGEEIALACRD 153

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A1_SCHPO (Eukaryotic translation initiation factor 5A-1 OS=Schizosaccharomyces pombe GN=tif51a PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.282e-19
Identity = 36/62 (58.06%), Postives = 50/62 (80.65%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H V +DIFNG+K ED+ PS+HN DVP V R +YQL++I +DG+++L+T +G TKDD+RLP
Sbjct:   57 KVHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLP 117

HSP 2 Score: 35.8094 bits (81), Expect = 3.282e-19
Identity = 14/31 (45.16%), Postives = 24/31 (77.42%), Query Frame = -2

Query:  299 SQIKDGFESGKDLVVSVQCAMGEEQINALKD 391
            ++I++GF+ G+DL+++V  AMGEE   A +D
Sbjct:  123 NEIEEGFDEGRDLIITVVSAMGEETALACRD 153

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A2_PONAB (Eukaryotic translation initiation factor 5A-2 OS=Pongo abelii GN=EIF5A2 PE=2 SV=3)

HSP 1 Score: 85.1149 bits (209), Expect = 7.237e-19
Identity = 40/62 (64.52%), Postives = 49/62 (79.03%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP
Sbjct:   55 KVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLP 115

HSP 2 Score: 30.0314 bits (66), Expect = 7.237e-19
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = -2

Query:  302 QIKDGFESGKDLVVSVQCAMGEEQINALK 388
            +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:  122 EIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A2_MOUSE (Eukaryotic translation initiation factor 5A-2 OS=Mus musculus GN=Eif5a2 PE=2 SV=3)

HSP 1 Score: 85.1149 bits (209), Expect = 7.237e-19
Identity = 40/62 (64.52%), Postives = 49/62 (79.03%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP
Sbjct:   55 KVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLP 115

HSP 2 Score: 30.0314 bits (66), Expect = 7.237e-19
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = -2

Query:  302 QIKDGFESGKDLVVSVQCAMGEEQINALK 388
            +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:  122 EIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A2_HUMAN (Eukaryotic translation initiation factor 5A-2 OS=Homo sapiens GN=EIF5A2 PE=1 SV=3)

HSP 1 Score: 85.1149 bits (209), Expect = 7.237e-19
Identity = 40/62 (64.52%), Postives = 49/62 (79.03%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP
Sbjct:   55 KVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLP 115

HSP 2 Score: 30.0314 bits (66), Expect = 7.237e-19
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = -2

Query:  302 QIKDGFESGKDLVVSVQCAMGEEQINALK 388
            +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:  122 EIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A_DROME (Eukaryotic translation initiation factor 5A OS=Drosophila melanogaster GN=eIF-5A PE=1 SV=2)

HSP 1 Score: 82.8037 bits (203), Expect = 5.907e-18
Identity = 37/62 (59.68%), Postives = 50/62 (80.65%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H VGIDIF+ KK EDI PS+HN DVP+V R D QLI IS+D F++L+TE+G+ ++DL++P
Sbjct:   56 KVHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVP 117

HSP 2 Score: 29.261 bits (64), Expect = 5.907e-18
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = -2

Query:  302 QIKDGFESGKDLVVSVQCAMGEEQINALK 388
            Q++  F+SGKDL+ +V  A GEE + A+K
Sbjct:  124 QLRLDFDSGKDLLCTVLKACGEECVIAIK 152

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A_CANAL (Eukaryotic translation initiation factor 5A OS=Candida albicans GN=ANB1 PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 5.907e-18
Identity = 36/62 (58.06%), Postives = 53/62 (85.48%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H V IDIF GKKLED+ PS+HN +VP+V+R ++QL+DI +DG++SL+T +G+TKDD+++P
Sbjct:   55 KVHLVAIDIFTGKKLEDLSPSTHNMEVPNVSRQEFQLLDI-DDGYLSLMTADGDTKDDVKVP 115

HSP 2 Score: 29.6462 bits (65), Expect = 5.907e-18
Identity = 12/30 (40.00%), Postives = 21/30 (70.00%), Query Frame = -2

Query:  299 QIKDGFESGKDLVVSVQCAMGEEQINALKD 388
            +++  F+ GKDL+V++  AMGEE   + K+
Sbjct:  122 KLQSEFDEGKDLIVTIISAMGEEAAISYKE 151

[Close]

BLAST of CN182135 vs. ExPASy Swiss-Prot
Match: IF5A2_CHICK (Eukaryotic translation initiation factor 5A-2 OS=Gallus gallus GN=EIF5A2 PE=1 SV=3)

HSP 1 Score: 88.5817 bits (218), Expect = 2.588e-17
Identity = 41/62 (66.13%), Postives = 51/62 (82.26%), Query Frame = -1

Query:  411 KCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLP 596
            K H VGIDIFNGKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE+G  ++DL+LP
Sbjct:   55 KVHLVGIDIFNGKKYEDICPSTHNMDVPNIKRNDYQLIGI-QDGYLSLLTESGEVREDLKLP 115

The following BLAST results are available for this feature:

BLAST of CN182135 vs. ExPASy Swiss-Prot

BLAST of CN182135 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 43


Match Name	E-value	Identity	Description
IF5A_SPOFR	1.006e-21	63.49	Eukaryotic translation initiation factor 5A OS=Spo...	[more]
IF5A_SPOEX	1.006e-21	63.49	Eukaryotic translation initiation factor 5A OS=Spo...	[more]
IF5A2_SCHPO	1.147e-19	58.06	Eukaryotic translation initiation factor 5A-2 OS=S...	[more]
IF5A1_SCHPO	3.282e-19	58.06	Eukaryotic translation initiation factor 5A-1 OS=S...	[more]
IF5A2_PONAB	7.237e-19	64.52	Eukaryotic translation initiation factor 5A-2 OS=P...	[more]
IF5A2_MOUSE	7.237e-19	64.52	Eukaryotic translation initiation factor 5A-2 OS=M...	[more]
IF5A2_HUMAN	7.237e-19	64.52	Eukaryotic translation initiation factor 5A-2 OS=H...	[more]
IF5A_DROME	5.907e-18	59.68	Eukaryotic translation initiation factor 5A OS=Dro...	[more]
IF5A_CANAL	5.907e-18	58.06	Eukaryotic translation initiation factor 5A OS=Can...	[more]
IF5A2_CHICK	2.588e-17	66.13	Eukaryotic translation initiation factor 5A-2 OS=G...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS04_0002A02_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0002A02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN182135 ID=CN182135; Name=CN182135; organism=Citrus sinensis; type=EST; length=596bp
GAGGGTAAATTGCATGTTTGAAACAACCAAAAGGTGCTTCGAAGTGTATA
TACAGTCACGTTGGCGGATACAACTTCTGCACACCGAGAACAACCATGTT
TCAACTTTCAAGCAATCAAACAGAATAAAAATACTGTCGATAGAAAACAA
TTAATAATATGGTAAGAGTCATTTTATATGAAATGATGGCATTCATCTTG
AAACCGGACACCAGTAAAGCTACACTACTATCCACCTTCCCACAGCTTTC
AGCAATCTGCATTCTCGATCAAGCAAAAGTCTTTTACTTGGGGCCAATGT
CCTTCAGGGCATTGATCTGCTCCTCTCCCATAGCACACTGAACTGAGACA
ACCAGATCTTTCCCACTCTCAAATCCATCCTTGATCTGTGAAAGCAGATT
TTCATCAGTTTGGAAGTCTCAGGTCATCCTTGGTATTGCCATTCTCAGTC
AGCAGGCTAACAAATCCATCCTCAGAGATATCAATCAGCTGGTAGTCAGT
GCGAGTGACATGAGGAACATCACAGTTGTGGGAAGAGGGGACAATATCCT
CAAGCTTCTTGCCATTGAAGATGTCAATTCCTACAAAGTGACACTT