CN181762 | Citrus Genome Database

Overview


Name	CN181762
Unique Name	CN181762
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	502

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: FRMA_ECO57 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O157:H7 GN=frmA PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.690e-18
Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = -2

Query:  292 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 501
            +PI++V+ ++   G+D + EC GN++ M +A E  H GWG +V++GV        T+P  ++  R  KG+
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGS 312

HSP 2 Score: 49.6766 bits (117), Expect = 6.690e-18
Identity = 22/54 (40.74%), Postives = 34/54 (62.96%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG  K R+ LP +V+  M   ++LE F+TH +   EIN AF+ M +G+ +R +I
Sbjct:  314 FGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: FRMA_ECO24 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.690e-18
Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = -2

Query:  292 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 501
            +PI++V+ ++   G+D + EC GN++ M +A E  H GWG +V++GV        T+P  ++  R  KG+
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGS 312

HSP 2 Score: 49.6766 bits (117), Expect = 6.690e-18
Identity = 22/54 (40.74%), Postives = 34/54 (62.96%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG  K R+ LP +V+  M   ++LE F+TH +   EIN AF+ M +G+ +R +I
Sbjct:  314 FGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADHP_RABIT (Alcohol dehydrogenase class-2 isozyme 1 OS=Oryctolagus cuniculus GN=ADH2-1 PE=2 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 1.470e-17
Identity = 31/70 (44.29%), Postives = 42/70 (60.00%), Query Frame = -2

Query:  292 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 501
            +P+Q+VI EMTNGGVD +++C G  + M +  +C   GWG    VGV   D      PI ++  RTLKGT
Sbjct:  254 KPVQDVIVEMTNGGVDFAIDCAGGSEVMKATVDCATVGWGSCTFVGVNLADKGLTISPIELILGRTLKGT 323

HSP 2 Score: 41.5874 bits (96), Expect = 1.470e-17
Identity = 17/54 (31.48%), Postives = 33/54 (61.11%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG +   T +P +V  Y N + +L+  +TH +PF +IN+A   + +G+ +R ++
Sbjct:  325 FGGWDAET-VPKLVSDYKNGKFDLDALVTHTLPFDKINEALNLLDQGKSIRTVL 377

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLI (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain K12) GN=frmA PE=1 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 1.470e-17
Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = -2

Query:  292 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 501
            +PI++V+ ++   G+D + EC GN++ M +A E  H GWG +V++GV        T+P  ++  R  KG+
Sbjct:  243 KPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAVAGQEISTRPFQLVTGRVWKGS 312

HSP 2 Score: 49.6766 bits (117), Expect = 1.470e-17
Identity = 22/54 (40.74%), Postives = 34/54 (62.96%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG  K R+ LP +V+  M   ++LE F+TH +   EIN AF+ M +G+ +R +I
Sbjct:  314 FGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVI 367

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: FADH_PICPA (S-(hydroxymethyl)glutathione dehydrogenase OS=Pichia pastoris GN=FLD1 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.910e-17
Identity = 22/67 (32.84%), Postives = 40/67 (59.70%), Query Frame = -2

Query:  295 IQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKG 495
            I + + ++T+GG D + +CTGN+  M +A E  H GWG ++++GV +      T+P  ++  R  +G
Sbjct:  254 IVDYLIDITDGGFDYTFDCTGNVQVMRNALESCHKGWGESIIIGVAAAGKEISTRPFQLVTGRVWRG 320

HSP 2 Score: 52.7582 bits (125), Expect = 1.910e-17
Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = -3

Query:  120 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCIISM 287
            FG  K RT +PS+V  Y++ ++++++FITHR     INKAF  M  G  +R +I+M
Sbjct:  323 FGGIKGRTQMPSLVQDYLDGKIKVDEFITHRHDLDNINKAFHDMHAGNCIRAVITM 378

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADHL7_ARATH (Alcohol dehydrogenase-like 7 OS=Arabidopsis thaliana GN=At5g42250 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.482e-17
Identity = 23/56 (41.07%), Postives = 41/56 (73.21%), Query Frame = -3

Query:  120 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCIISM 287
            FG  K +T +P ++  Y++ +LEL+KF+TH + F EIN AF+ +++G+ +RC++ M
Sbjct:  334 FGGLKAKTHIPILLKRYLSNELELDKFVTHEMKFEEINDAFQLLLEGKCIRCVLWM 389

HSP 2 Score: 47.7506 bits (112), Expect = 2.482e-17
Identity = 25/75 (33.33%), Postives = 38/75 (50.67%), Query Frame = -2

Query:  274 IQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNE-RTLKGTLIRELQ 495
            + EVI EMT+GG D   EC G+   +  A+ C   GWG  + +GV    +       +VL+  + L G+L   L+
Sbjct:  264 VSEVINEMTDGGADYCFECVGSSSLVQEAYACCRQGWGKTITLGVDKPGSQICLDSFDVLHHGKILMGSLFGGLK 338

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADHI_RHOS4 (Alcohol dehydrogenase class-3 OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) GN=adhI PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.484e-17
Identity = 22/54 (40.74%), Postives = 35/54 (64.81%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG  + RTD+P +VD YM  +++++  ITH +   EINK F+ M  GE +R ++
Sbjct:  321 FGGARGRTDVPKIVDWYMEGKIQIDPMITHILSLEEINKGFDLMHAGESIRSVV 374

HSP 2 Score: 53.1434 bits (126), Expect = 2.484e-17
Identity = 25/75 (33.33%), Postives = 39/75 (52.00%), Query Frame = -2

Query:  292 EVIAEMTN---------GGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 489
            +V+A + N         GG D + +CTGN+  M  A E  H GWG ++++GV    A   T+P  ++  R  KG+
Sbjct:  245 DVVAHLVNMTKTPFDQIGGADYTFDCTGNVKVMRQALEACHRGWGQSIVIGVAPAGAEIQTRPFQLVTGRVWKGS 319

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADH1_CHICK (Alcohol dehydrogenase 1 OS=Gallus gallus GN=ADH1 PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 3.230e-17
Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = -2

Query:  265 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGTLIRELQTAD 501
            +PI EV+ EMT  GVD S E  G I+ M +A    H+ +GV+V+VGVP         P+ + + RT KG++    ++ D
Sbjct:  250 KPIHEVLTEMTGQGVDYSFEVIGRIETMTAALASCHNNYGVSVIVGVPPAAQKISFDPMLIFSGRTWKGSVFGGWKSKD 328

HSP 2 Score: 48.1358 bits (113), Expect = 3.230e-17
Identity = 19/54 (35.19%), Postives = 35/54 (64.81%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG +K +  +P +V  YM K+  L+  ITH +PF++IN+ F+ +  G+ +R ++
Sbjct:  321 FGGWKSKDAVPKLVADYMKKKFVLDPLITHTLPFTKINEGFDLLRTGKSIRSVL 374

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADH1_RANPE (Alcohol dehydrogenase 1 OS=Rana perezi PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.230e-17
Identity = 30/78 (38.46%), Postives = 41/78 (52.56%), Query Frame = -2

Query:  265 PIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGTLIRELQTAD 498
            PI EV+A+MT+ GVD + E  GN   M SA    H G G  V+VG+    AV    P+ +L  R L G +    ++ D
Sbjct:  250 PIHEVLAKMTDDGVDYAFEVIGNTTVMTSALSSSHFGCGKTVIVGLAPSSAVMSFDPLLILTGRILTGAVFGGWKSKD 327

HSP 2 Score: 53.5286 bits (127), Expect = 3.230e-17
Identity = 21/55 (38.18%), Postives = 37/55 (67.27%), Query Frame = -3

Query:  123 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCIIS 287
            FG +K + D+P +V  Y+NK+ + +  ITH +PF +IN+ FE +  G+ +R I++
Sbjct:  320 FGGWKSKDDVPKLVRDYLNKKFDFDPLITHYMPFEKINEGFELLRNGKSIRTILT 374

[Close]

BLAST of CN181762 vs. ExPASy Swiss-Prot
Match: ADHQ_RABIT (Alcohol dehydrogenase class-2 isozyme 2 OS=Oryctolagus cuniculus GN=ADH2-2 PE=2 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 5.457e-17
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = -2

Query:  292 RPIQEVIAEMTNGGVDRSVECTGNIDNMISAFECVHDGWGVAVLVGVPSKDAVFMTKPINVLNERTLKGT 501
            +P+Q+VI EMTNGGVD +++C G  + M +  +C   GWG    VGV   D      P+ ++  RTL+G+
Sbjct:  254 KPVQDVIVEMTNGGVDFAIDCAGGSEVMKATVDCTTVGWGSCTFVGVNVNDKGLTISPVELILGRTLRGS 323

HSP 2 Score: 42.3578 bits (98), Expect = 5.457e-17
Identity = 19/54 (35.19%), Postives = 33/54 (61.11%), Query Frame = -3

Query:  126 FGNYKPRTDLPSVVDMYMNKQLELEKFITHRIPFSEINKAFEYMVKGEGLRCII 287
            FG +   T +P +V  Y N +  LE  +TH +PF +IN+A + + +G+ +R I+
Sbjct:  325 FGGWDVDT-VPKLVSDYKNGKFNLEALVTHTLPFEKINEALDLLKQGKSIRTIL 377

The following BLAST results are available for this feature:

BLAST of CN181762 vs. ExPASy Swiss-Prot

BLAST of CN181762 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 100


Match Name	E-value	Identity	Description
FRMA_ECO57	6.690e-18	34.29	S-(hydroxymethyl)glutathione dehydrogenase OS=Esch...	[more]
FRMA_ECO24	6.690e-18	34.29	S-(hydroxymethyl)glutathione dehydrogenase OS=Esch...	[more]
ADHP_RABIT	1.470e-17	44.29	Alcohol dehydrogenase class-2 isozyme 1 OS=Oryctol...	[more]
FRMA_ECOLI	1.470e-17	34.29	S-(hydroxymethyl)glutathione dehydrogenase OS=Esch...	[more]
FADH_PICPA	1.910e-17	32.84	S-(hydroxymethyl)glutathione dehydrogenase OS=Pich...	[more]
ADHL7_ARATH	2.482e-17	41.07	Alcohol dehydrogenase-like 7 OS=Arabidopsis thalia...	[more]
ADHI_RHOS4	2.484e-17	40.74	Alcohol dehydrogenase class-3 OS=Rhodobacter sphae...	[more]
ADH1_CHICK	3.230e-17	37.97	Alcohol dehydrogenase 1 OS=Gallus gallus GN=ADH1 P...	[more]
ADH1_RANPE	3.230e-17	38.46	Alcohol dehydrogenase 1 OS=Rana perezi PE=1 SV=1	[more]
ADHQ_RABIT	5.457e-17	40.00	Alcohol dehydrogenase class-2 isozyme 2 OS=Oryctol...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS04_0001H03_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001H03, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN181762 ID=CN181762; Name=CN181762; organism=Citrus sinensis; type=EST; length=502bp
AAGACATATACTTAGGGACTGATGCATCAAGTTACAAAGATGAAGTCAAT
AGCAGGTGCTTGCCCACCTACTGTTGTTATTTACTTGATTATTCTTTCCC
TTTTTCGCTCCTAGTCTTCCATGCTGATAATGCACCTCAAACCCTCCCCT
TTCACCATGTACTCGAAGGCTTTATTGATTTCCGAGAAAGGGATGCGATG
GGTTATAAACTTCTCCAGTTCCAGTTGCTTGTTCATGTACATATCAACGA
CAGAGGGCAGATCAGTCCGCGGTTTGTAGTTCCCGAATTAATGTTCCTTT
GAGGGTTCTTTCGTTAAGTACATTGATCGGCTTGGTCATGAACACTGCAT
CCTTACTGGGGACTCCAACGAGCACAGCTACACCCCATCCATCATGAACA
CACTCGAAGGCTGAGATCATGTTATCAATGTTGCCGGTGCACTCCACACT
TCTGTCAACTCCACCGTTCGTCATCTCTGCAATTACCTCTTGAATTGGTC
TG