CX300700

Overview
NameCX300700
Unique NameCX300700
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length401
Libraries
Library NameType
PhyRootSw1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_METM5 (Proteasome-activating nucleotidase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=pan PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.146e-33
Identity = 45/78 (57.69%), Postives = 59/78 (75.64%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNV 385
            S   GDREVQRT+++LL ++DGF S   +K+IAATNR DILDPA++R GR DR IE+  P E+ R  IL+IH+ KMN+
Sbjct:  260 SLTGGDREVQRTLMQLLAEMDGFDSRGDVKIIAATNRPDILDPAILRPGRFDRIIEISMPDEDGRLDILKIHTEKMNL 337

HSP 2 Score: 66.6254 bits (161), Expect = 3.146e-33
Identity = 31/40 (77.50%), Postives = 36/40 (90.00%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFDRLT 143
            IG+GAKLVRD F+LAKEKSPCIIFIDEIDA+ +KR + LT
Sbjct:  223 IGEGAKLVRDVFKLAKEKSPCIIFIDEIDAVASKRTESLT 262
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_DROME (26S protease regulatory subunit 8 OS=Drosophila melanogaster GN=Pros45 PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 4.098e-33
Identity = 57/79 (72.15%), Postives = 64/79 (81.01%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            GD EVQRTMLELLNQLDGF +   IKVI ATNR DILDPAL+R GR+DRKIE P P+EEAR  IL+IHSRKMN+   +N
Sbjct:  264 GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGIN 342

HSP 2 Score: 47.3654 bits (111), Expect = 4.098e-33
Identity = 19/37 (51.35%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+G+++VR+ F +A+E +P IIF+DEID+IG+ R +
Sbjct:  223 IGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRIE 259
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_MANSE (26S protease regulatory subunit 8 OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 4.098e-33
Identity = 57/79 (72.15%), Postives = 64/79 (81.01%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            GD EVQRTMLELLNQLDGF +   IKVI ATNR DILDPAL+R GR+DRKIE P P+EEAR  IL+IHSRKMN+   +N
Sbjct:  261 GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGIN 339

HSP 2 Score: 47.3654 bits (111), Expect = 4.098e-33
Identity = 19/37 (51.35%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+G+++VR+ F +A+E +P IIF+DEID+IG+ R +
Sbjct:  220 IGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRIE 256
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4_ORYSJ (26S protease regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica GN=TBP2 PE=2 SV=2)

HSP 1 Score: 103.219 bits (256), Expect = 5.330e-33
Identity = 51/79 (64.56%), Postives = 60/79 (75.95%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRKIE P P  + R RI QIH+ KM +  DVN
Sbjct:  309 GEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLADDVN 387

HSP 2 Score: 57.3806 bits (137), Expect = 5.330e-33
Identity = 25/37 (67.57%), Postives = 32/37 (86.49%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A E SP I+FIDEIDA+GTKR+D
Sbjct:  268 LGDGPKLVRELFRVADELSPSIVFIDEIDAVGTKRYD 304
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_METVS (Proteasome-activating nucleotidase OS=Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) GN=pan PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.336e-33
Identity = 44/78 (56.41%), Postives = 58/78 (74.36%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNV 385
            S   GDREVQRT+++LL ++DGF S   +K+IAATNR DILDPA++R GR DR IE+  P E+ R  I +IH+ KMN+
Sbjct:  260 SLTGGDREVQRTLMQLLAEMDGFDSRGDVKIIAATNRPDILDPAILRPGRFDRIIEIAAPDEDGRLEIFKIHTDKMNI 337

HSP 2 Score: 66.6254 bits (161), Expect = 5.336e-33
Identity = 31/40 (77.50%), Postives = 36/40 (90.00%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFDRLT 143
            IG+GAKLVRD F+LAKEKSPCIIFIDEIDA+ +KR + LT
Sbjct:  223 IGEGAKLVRDVFKLAKEKSPCIIFIDEIDAVASKRTESLT 262
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4_RAT (26S protease regulatory subunit 4 OS=Rattus norvegicus GN=Psmc1 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 6.943e-33
Identity = 50/82 (60.98%), Postives = 61/82 (74.39%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDV 397
            S   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRKIE P P E+ + RI QIH+ +M +  DV
Sbjct:  297 SNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDV 378

HSP 2 Score: 57.7658 bits (138), Expect = 6.943e-33
Identity = 25/37 (67.57%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A+E +P I+FIDEIDAIGTKR+D
Sbjct:  260 LGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYD 296
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4_MOUSE (26S protease regulatory subunit 4 OS=Mus musculus GN=Psmc1 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 6.943e-33
Identity = 50/82 (60.98%), Postives = 61/82 (74.39%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDV 397
            S   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRKIE P P E+ + RI QIH+ +M +  DV
Sbjct:  297 SNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDV 378

HSP 2 Score: 57.7658 bits (138), Expect = 6.943e-33
Identity = 25/37 (67.57%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A+E +P I+FIDEIDAIGTKR+D
Sbjct:  260 LGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYD 296
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4_HUMAN (26S protease regulatory subunit 4 OS=Homo sapiens GN=PSMC1 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 6.943e-33
Identity = 50/82 (60.98%), Postives = 61/82 (74.39%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDV 397
            S   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRKIE P P E+ + RI QIH+ +M +  DV
Sbjct:  297 SNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDV 378

HSP 2 Score: 57.7658 bits (138), Expect = 6.943e-33
Identity = 25/37 (67.57%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A+E +P I+FIDEIDAIGTKR+D
Sbjct:  260 LGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYD 296
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_ENCCU (26S protease regulatory subunit 8 homolog OS=Encephalitozoon cuniculi GN=RPT6 PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 9.038e-33
Identity = 56/78 (71.79%), Postives = 63/78 (80.77%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNV 385
            S    D EVQRTMLELLNQLDGF S + IKVI ATNR DILDPAL+R+GR+DRKIE P P+E AR  IL+IHSRKMN+
Sbjct:  308 SNKGSDSEVQRTMLELLNQLDGFESHNNIKVIMATNRIDILDPALLRTGRIDRKIEFPPPNESARLEILKIHSRKMNL 385

HSP 2 Score: 47.7506 bits (112), Expect = 9.038e-33
Identity = 21/37 (56.76%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+G++LVR+ F +A+E +P IIF+DEID+IG+ R D
Sbjct:  271 IGEGSRLVRELFIMAREHAPSIIFMDEIDSIGSTRGD 307
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4A_ARATH (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana GN=RPT2A PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 9.041e-33
Identity = 51/79 (64.56%), Postives = 60/79 (75.95%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRKIE P P  + R RI QIH+ KM +  DVN
Sbjct:  304 GEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLSEDVN 382

HSP 2 Score: 56.225 bits (134), Expect = 9.041e-33
Identity = 24/37 (64.86%), Postives = 32/37 (86.49%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A + SP I+FIDEIDA+GTKR+D
Sbjct:  263 LGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYD 299
The following BLAST results are available for this feature:
BLAST of CX300700 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 270
Match NameE-valueIdentityDescription
PSMR_METM53.146e-3357.69Proteasome-activating nucleotidase OS=Methanococcu... [more]
PRS8_DROME4.098e-3372.1526S protease regulatory subunit 8 OS=Drosophila me... [more]
PRS8_MANSE4.098e-3372.1526S protease regulatory subunit 8 OS=Manduca sexta... [more]
PRS4_ORYSJ5.330e-3364.5626S protease regulatory subunit 4 homolog OS=Oryza... [more]
PSMR_METVS5.336e-3356.41Proteasome-activating nucleotidase OS=Methanococcu... [more]
PRS4_RAT6.943e-3360.9826S protease regulatory subunit 4 OS=Rattus norveg... [more]
PRS4_MOUSE6.943e-3360.9826S protease regulatory subunit 4 OS=Mus musculus ... [more]
PRS4_HUMAN6.943e-3360.9826S protease regulatory subunit 4 OS=Homo sapiens ... [more]
PRS8_ENCCU9.038e-3371.7926S protease regulatory subunit 8 homolog OS=Encep... [more]
PRS4A_ARATH9.041e-3364.5626S proteasome regulatory subunit 4 homolog A OS=A... [more]

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Properties
Property NameValue
Genbank descriptionC08001C09SK PhyRootSw1 Citrus sinensis cDNA clone C08001C09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX300700 ID=CX300700; Name=CX300700; organism=Citrus sinensis; type=EST; length=401bp
CTCGTGCCGAATTCGGCACGAGGATTGGAGATGGAGCCAAACTAGTTCGT
GATGCCTTTCAACTTGCAAAGGAGAAGTCCCCCTGCATTATTTTCATCGA
TGAAATTGACGCAATTGGTACAAAGCGGTTTGACAGGCTCACTGTGACTG
CAGTGAAGTGAGTGGAGACAGGGAGGTGCAGCGAACTATGTTAGAGTTGC
TCAATCAGCTAGATGGATTTAGTAGCGATGATCGGATTAAGGTGATAGCA
GCAACAAATCGTGCTGATATCCTGGACCCTGCTCTTATGCGATCTGGTCG
TTTGGATCGCAAGATCGAGTTACCACATCCGAGTGAGGAAGCTAGAGCTC
GCATTCTGCAAATCCATTCGAGGAAGATGAATGTTCACCCAGATGTCAAC
T
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