CX300700

Overview
NameCX300700
Unique NameCX300700
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length401
Libraries
Library NameType
PhyRootSw1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_BOVIN (26S protease regulatory subunit 8 OS=Bos taurus GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.535e-32
Identity = 56/79 (70.89%), Postives = 63/79 (79.75%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            GD EVQRTMLELLNQLDGF +   IKVI ATNR DILD AL+R GR+DRKIE P P+EEAR  IL+IHSRKMN+   +N
Sbjct:  265 GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGIN 343

HSP 2 Score: 48.521 bits (114), Expect = 1.535e-32
Identity = 20/37 (54.05%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+GA++VR+ F +A+E +P IIF+DEID+IG+ R +
Sbjct:  224 IGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLE 260
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS4_DICDI (26S protease regulatory subunit 4 homolog OS=Dictyostelium discoideum GN=psmC1 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.997e-32
Identity = 49/83 (59.04%), Postives = 63/83 (75.90%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            S+  G+RE+QRTMLELLNQLDGF +   +KVI ATNR + LDPAL+R GR+DRKIE P P  + + +I +IH+ KMN+  DVN
Sbjct:  296 SQSGGEREIQRTMLELLNQLDGFDARTDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDIKTKRKIFEIHTAKMNLSEDVN 378

HSP 2 Score: 55.8398 bits (133), Expect = 1.997e-32
Identity = 24/37 (64.86%), Postives = 32/37 (86.49%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            +GDG KLVR+ F++A E +P I+FIDEIDA+GTKR+D
Sbjct:  259 LGDGPKLVRELFRVADECAPSIVFIDEIDAVGTKRYD 295
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8B_ARATH (26S protease regulatory subunit 8 homolog B OS=Arabidopsis thaliana GN=RPT6B PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 4.414e-32
Identity = 54/83 (65.06%), Postives = 67/83 (80.72%), Query Frame = 2 
Query:  137 AHCDCSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNV 385
            A  +    +GD EVQRTMLELLNQLDGF + ++IKV+ ATNR DILD AL+R GR+DRKIE P+P+EE+R  IL+IHSRKMN+
Sbjct:  269 ARMESGSGNGDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNL 351

HSP 2 Score: 46.9802 bits (110), Expect = 4.414e-32
Identity = 19/37 (51.35%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+G+++VR+ F +A+E +P IIF+DEID+IG+ R +
Sbjct:  236 IGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSARME 272
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8A_ARATH (26S protease regulatory subunit 8 homolog A OS=Arabidopsis thaliana GN=RPT6A PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 4.414e-32
Identity = 54/83 (65.06%), Postives = 67/83 (80.72%), Query Frame = 2 
Query:  137 AHCDCSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNV 385
            A  +    +GD EVQRTMLELLNQLDGF + ++IKV+ ATNR DILD AL+R GR+DRKIE P+P+EE+R  IL+IHSRKMN+
Sbjct:  269 ARMESGSGNGDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNL 351

HSP 2 Score: 46.9802 bits (110), Expect = 4.414e-32
Identity = 19/37 (51.35%), Postives = 31/37 (83.78%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+G+++VR+ F +A+E +P IIF+DEID+IG+ R +
Sbjct:  236 IGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSARME 272
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_XENLA (26S protease regulatory subunit 8 OS=Xenopus laevis GN=psmc5 PE=2 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 5.740e-32
Identity = 56/79 (70.89%), Postives = 63/79 (79.75%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            GD EVQRTMLELLNQLDGF +   IKVI ATNR DILD AL+R GR+DRKIE P P+EEAR  IL+IHSRKMN+   +N
Sbjct:  259 GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGIN 337

HSP 2 Score: 46.595 bits (109), Expect = 5.740e-32
Identity = 19/34 (55.88%), Postives = 30/34 (88.24%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTK 125
            IG+GA++VR+ F +A+E +P IIF+DEID+IG++
Sbjct:  219 IGEGARMVRELFVMAREHAPSIIFMDEIDSIGSR 252
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_METKA (Proteasome-activating nucleotidase OS=Methanopyrus kandleri GN=pan PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 7.482e-32
Identity = 50/80 (62.50%), Postives = 61/80 (76.25%), Query Frame = 2 
Query:  161 SGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            SGDREVQRT+ +LL ++DGF   D IKVIAATNR DILDPAL+R GR DR I++P P EE R  I +IH+R MN+  DV+
Sbjct:  294 SGDREVQRTLTQLLAEMDGFDPLDDIKVIAATNRKDILDPALLRPGRFDRHIKIPLPDEEGRYEIFKIHTRDMNLAEDVD 373

HSP 2 Score: 55.8398 bits (133), Expect = 7.482e-32
Identity = 25/35 (71.43%), Postives = 32/35 (91.43%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKR 128
            IG+GA+LVR+ F+LA+EK+P IIFIDEIDAIG +R
Sbjct:  254 IGEGARLVRELFELAREKAPSIIFIDEIDAIGARR 288
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_AERPE (Proteasome-activating nucleotidase OS=Aeropyrum pernix GN=pan PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 7.488e-32
Identity = 50/80 (62.50%), Postives = 61/80 (76.25%), Query Frame = 2 
Query:  161 SGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            SGDREVQRTML+LL +LDGF   D +KVIAATNR D++DPA++R GR DR IE+P PS   R  IL IH+RK  + PDV+
Sbjct:  254 SGDREVQRTMLQLLAELDGFDPLDNVKVIAATNRLDLIDPAVLRPGRFDRIIEVPLPSLRGRLEILGIHTRKAKMAPDVD 333

HSP 2 Score: 55.8398 bits (133), Expect = 7.488e-32
Identity = 25/37 (67.57%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFD 134
            IG+GA+LVR+ F+LA+EK+P I+FIDEIDAI +KR D
Sbjct:  214 IGEGARLVREIFRLAREKAPSILFIDEIDAIASKRVD 250
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_METMJ (Proteasome-activating nucleotidase OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=pan PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.270e-31
Identity = 48/88 (54.55%), Postives = 65/88 (73.86%), Query Frame = 2 
Query:  137 AHCDCSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            AH + S  SGDREVQRT+++LL ++DGF +   +K++AATNR DILD AL+R GR DR IE+P P  + R  IL+IH++ MN+  DVN
Sbjct:  260 AHRNDSTTSGDREVQRTLMQLLAEMDGFDNRGDVKIVAATNRIDILDRALLRPGRFDRMIEIPLPDHQGRLAILKIHTQYMNIGEDVN 347

HSP 2 Score: 55.0694 bits (131), Expect = 1.270e-31
Identity = 26/40 (65.00%), Postives = 32/40 (80.00%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFDRLT 143
            IG+GA+LVR+ F LAK+++P IIFIDEIDAIG  R D  T
Sbjct:  228 IGEGARLVRELFDLAKQRAPSIIFIDEIDAIGAHRNDSTT 267
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PSMR_ARCFU (Proteasome-activating nucleotidase OS=Archaeoglobus fulgidus GN=pan PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.654e-31
Identity = 49/83 (59.04%), Postives = 63/83 (75.90%), Query Frame = 2 
Query:  152 SEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            S+ SGDREVQRTM++LL +LDGF     +KVI ATNR DILDPA++R GR DR IE+P P+ E R +I +IH+RKM +  DV+
Sbjct:  253 SDTSGDREVQRTMMQLLAELDGFDPRGDVKVIGATNRIDILDPAILRPGRFDRIIEVPLPTFEGRIQIFKIHTRKMKLAEDVD 335

HSP 2 Score: 55.4546 bits (132), Expect = 1.654e-31
Identity = 25/35 (71.43%), Postives = 31/35 (88.57%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKR 128
            IG+GA+LVR+ FQLAKEK+P IIFIDE+DAI  +R
Sbjct:  216 IGEGARLVREVFQLAKEKAPSIIFIDELDAIAARR 250
[Close]
BLAST of CX300700 vs. ExPASy Swiss-Prot
Match: PRS8_DICDI (26S protease regulatory subunit 8 OS=Dictyostelium discoideum GN=psmC5 PE=1 SV=2)

HSP 1 Score: 108.227 bits (269), Expect = 3.652e-31
Identity = 53/79 (67.09%), Postives = 61/79 (77.22%), Query Frame = 2 
Query:  164 GDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIELPHPSEEARARILQIHSRKMNVHPDVN 400
            GD EVQRTMLELLNQLDGF S   IKV+  TNR DILDPAL+R GR+DRKIE P+P +  R  IL+IHSRKMN+   +N
Sbjct:  262 GDSEVQRTMLELLNQLDGFESTKNIKVLMCTNRIDILDPALLRPGRIDRKIEFPNPGDAGRLDILKIHSRKMNLTRGIN 340

HSP 2 Score: 46.2098 bits (108), Expect = 3.652e-31
Identity = 19/35 (54.29%), Postives = 30/35 (85.71%), Query Frame = 3 
Query:   24 IGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKR 128
            IG+G+++VR+ F +A+E +P IIF+DEID+IG+ R
Sbjct:  220 IGEGSRMVRELFIMAREHAPSIIFMDEIDSIGSSR 254
The following BLAST results are available for this feature:
BLAST of CX300700 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 270
Match NameE-valueIdentityDescription
PRS8_BOVIN1.535e-3270.8926S protease regulatory subunit 8 OS=Bos taurus GN... [more]
PRS4_DICDI1.997e-3259.0426S protease regulatory subunit 4 homolog OS=Dicty... [more]
PRS8B_ARATH4.414e-3265.0626S protease regulatory subunit 8 homolog B OS=Ara... [more]
PRS8A_ARATH4.414e-3265.0626S protease regulatory subunit 8 homolog A OS=Ara... [more]
PRS8_XENLA5.740e-3270.8926S protease regulatory subunit 8 OS=Xenopus laevi... [more]
PSMR_METKA7.482e-3262.50Proteasome-activating nucleotidase OS=Methanopyrus... [more]
PSMR_AERPE7.488e-3262.50Proteasome-activating nucleotidase OS=Aeropyrum pe... [more]
PSMR_METMJ1.270e-3154.55Proteasome-activating nucleotidase OS=Methanoculle... [more]
PSMR_ARCFU1.654e-3159.04Proteasome-activating nucleotidase OS=Archaeoglobu... [more]
PRS8_DICDI3.652e-3167.0926S protease regulatory subunit 8 OS=Dictyostelium... [more]

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Properties
Property NameValue
Genbank descriptionC08001C09SK PhyRootSw1 Citrus sinensis cDNA clone C08001C09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX300700 ID=CX300700; Name=CX300700; organism=Citrus sinensis; type=EST; length=401bp
CTCGTGCCGAATTCGGCACGAGGATTGGAGATGGAGCCAAACTAGTTCGT
GATGCCTTTCAACTTGCAAAGGAGAAGTCCCCCTGCATTATTTTCATCGA
TGAAATTGACGCAATTGGTACAAAGCGGTTTGACAGGCTCACTGTGACTG
CAGTGAAGTGAGTGGAGACAGGGAGGTGCAGCGAACTATGTTAGAGTTGC
TCAATCAGCTAGATGGATTTAGTAGCGATGATCGGATTAAGGTGATAGCA
GCAACAAATCGTGCTGATATCCTGGACCCTGCTCTTATGCGATCTGGTCG
TTTGGATCGCAAGATCGAGTTACCACATCCGAGTGAGGAAGCTAGAGCTC
GCATTCTGCAAATCCATTCGAGGAAGATGAATGTTCACCCAGATGTCAAC
T
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